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               PA0035               
Key to diagram


View in GBrowse: 16 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name trpA  Alternate Gene Names
Locus tag PA0035 (View 6 updates) Submit an Update GenBank Identifier (GI) 15595233 (Precomputed BLAST results at BLINK )
Genomic location 37893 - 37087 (-) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name tryptophan synthase alpha chain (Class 1  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA00036
Systomonas PA0035
RefSeq NP_248725.1
GI 15595233
KEGG PA0035
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
trpA tryptophan synthase alpha chain
trpB tryptophan synthase beta chain
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13113
Genomic Context
Genomic context Explanation of Genomic Context Field - trpABI (Pseudomonas aeruginosa)
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Amino acid biosynthesis and metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0003849 3-deoxy-7-phosphoheptulonate synthase activity ISS
GO:0004834 tryptophan synthase activity IEA
Biological Process
GO:0006520 amino acid metabolic process IDA
GO:0009094 L-phenylalanine biosynthetic process IDA
GO:0006571 tyrosine biosynthetic process IDA
GO:0000162 tryptophan biosynthetic process IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0159 TrpA, Tryptophan synthase alpha chain [Amino acid transport and metabolism]. Amino acid transport and metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Phenylalanine, tyrosine and tryptophan biosynthesis PseudoCAP
phenylalanine, tyrosine and tryptophan biosynthesis, complete PseudoCyc COMPLETE-ARO-PWY
tryptophan biosynthesis PseudoCyc TRPSYN-PWY
pathways of chorismate PseudoCyc ALL-CHORISMATE-PWY
Glycine, serine and threonine metabolism KEGG pae00260 Computationally Generated
Phenylalanine, tyrosine and tryptophan biosynthesis KEGG pae00400 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Two-component system KEGG pae02020 Computationally Generated
Reactions
EC Number 4.2.1.20
Homology
BLINK Precomputed BLAST results at BLINK
Structure Features
Structure Features Explanation of Structure Features Field BL00167: Tryptophan synthase alpha chain signature
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00290 Trp_syntA, Tryptophan synthase alpha chain. 9 265 1e-102 23.0
PF01884 PcrB, PcrB family. 189 251 1e-06 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region TGCATCGGCGGCGGCTCCAACGCCATGGGCCTGTTCCACCCGTTCCTCGACGACGCCGGGGTGCAGATCGTCGGCGTGGAAGCCGCCGGCCACGGCATCG ACACCGGCAAGCACGCGGCCAGCCTGAACGGCGGGGTTCCCGGCGTGCTGCACGGCAACCGCACCTTCCTGCTGCAGGACGCGGATGGCCAGATCATCGA CGCACACTCCATCTCCGCCGGCCTCGACTATCCCGGCATCGGCCCGGAACACGCCTGGCTGCACGACACCGGCCGCGTCGAGTACACCTCGATCACCGAC GACGAAGCCCTGGAGGCCTTCCACACCTGCTGCCGCCTCGAAGGCATCATCCCGGCACTGGAAAGCTCCCATGCCCTGGCCGAGGTCTTCAAGCGTGCGC CCAGCCTGCCCAAGGAGCACATCATGGTGGTGAACCTGTCCGGTCGCGGCGACAAGGACATGCAGACCGTCATGCACCACATGCAACAGGAGTCGAAAGC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGAGCCGCCTGCAGACCCGCTTCGCCCAGCTCAAGCAGGAAAACCGCGCCGCCCTGGTGACCTTCGTCACCGCCGGCGACCCGGACTACGCGTCTTCCC TGGAAATCCTCAAAGGCCTGCCGGCGGCCGGAGCCGACGTGATCGAACTGGGCATGCCGTTCACCGATCCGATGGCGGACGGCCCGGCCATCCAGTTGGC CAACATCCGCGCCCTGGACGGCGGCCAGACCCTGGCCAGGACGTTGCAGATGGTCCGCGAATTCCGCAGCGGCGACAGCGAGACGCCCCTGGTGCTGATG GGCTACTTCAACCCGATCCACCACTACGGCGTCGAACGCTTCATCGCCGAGGCGAAGGAGGTGGGAGTGGACGGCCTGATCGTGGTCGACCTGCCGCCGG AGCACAACGAAGACCTCTGCCACCCGGCCCAGGCCGCCGGCATCGACTTCATCCGCCTGACCACCCCGACCACCGGCGACCAGCGCCTGCCGACGGTGCT AGAAGGCAGTTCCGGGTTCGTCTACTACGTGTCGGTGGCCGGCGTCACCGGCGCCAACGCGGCGACCCTGGAACACGTCGAGGAAGCGGTGGCGCGCCTG CGCCGGCATACCGACCTGCCGATCGGTATCGGCTTCGGCATCCGCAGCGCCGAACACGCCGCCGCGGTCGCGCGGTTGGCCGACGGCGTGGTGGTCGGCT CGGCGCTGATCGACCGGATCGCCAAGGCCCGCGACAATGCCCAGGCGGTCAAGGATGTCCTCGCCCTGTGCGGCGAGCTGGCCGAAGGCGTGCGCAACGC ACGCTGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MSRLQTRFAQLKQENRAALVTFVTAGDPDYASSLEILKGLPAAGADVIELGMPFTDPMADGPAIQLANIRALDGGQTLARTLQMVREFRSGDSETPLVLM GYFNPIHHYGVERFIAEAKEVGVDGLIVVDLPPEHNEDLCHPAQAAGIDFIRLTTPTTGDQRLPTVLEGSSGFVYYVSVAGVTGANAATLEHVEEAVARL RRHTDLPIGIGFGIRSAEHAAAVARLADGVVVGSALIDRIAKARDNAQAVKDVLALCGELAEGVRNAR

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0035 gene of Pseudomonas aeruginosa PAO1, Chromosome (37087 to 37893 bp)


Displaying the first five SNPs out of a total of 70 found in all isolate strains ( View all 70 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA7 Transition g.37090G>A
Silent
CGC(Arg) -> CGT(Arg) 		SNP-506691 View
Pseudomonas aeruginosa PA7 Transition g.37093T>C
Silent
GCA(Ala) -> GCG(Ala) 		SNP-506692 View
Pseudomonas aeruginosa PA7 Transversion g.37111G>C
Silent
GCC(Ala) -> GCG(Ala) 		SNP-506693 View
Pseudomonas aeruginosa PA7 Transition g.37117C>T
Silent
GAG(Glu) -> GAA(Glu) 		SNP-506694 View
Pseudomonas aeruginosa PA7 Transversion g.37126C>G
Silent
CTG(Leu) -> CTC(Leu) 		SNP-506695 View
Select to view SNPs in a specific strain
SNP Map
PubMed Nucleotide sequence of the genes for tryptophan synthase in Pseudomonas aeruginosa.
Hadero A, Crawford IP.
Mol Biol Evol. 1986 May;3(3):191-204.
PMID: 3127651
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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