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               PA0036               
Key to diagram


View in GBrowse: 16 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name trpB  Alternate Gene Names
Locus tag PA0036 (View 7 updates) Submit an Update GenBank Identifier (GI) 15595234 (Precomputed BLAST results at BLINK )
Genomic location 39098 - 37890 (-) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name tryptophan synthase beta chain (Class 1  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Cytoplasmic (Class 1Explanation of Localization Confidence)
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA00037
Systomonas PA0036
RefSeq NP_248726.1
GI 15595234
KEGG PA0036
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Amino acid biosynthesis and metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0016833 oxo-acid-lyase activity ISS
GO:0004834 tryptophan synthase activity IEA
Biological Process
GO:0006520 amino acid metabolic process IDA
GO:0009094 L-phenylalanine biosynthetic process IDA
GO:0006571 tyrosine biosynthetic process IDA
GO:0000162 tryptophan biosynthetic process IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0133 TrpB, Tryptophan synthase beta chain [Amino acid transport and metabolism]. Amino acid transport and metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Phenylalanine, tyrosine and tryptophan biosynthesis PseudoCAP
phenylalanine, tyrosine and tryptophan biosynthesis, complete PseudoCyc COMPLETE-ARO-PWY
tryptophan biosynthesis PseudoCyc TRPSYN-PWY
pathways of chorismate PseudoCyc ALL-CHORISMATE-PWY
Glycine, serine and threonine metabolism KEGG pae00260 Computationally Generated
Phenylalanine, tyrosine and tryptophan biosynthesis KEGG pae00400 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Two-component system KEGG pae02020 Computationally Generated
Reactions
EC Number 4.2.1.20
Homology
BLINK Precomputed BLAST results at BLINK
Structure Features
Structure Features Explanation of Structure Features Field BL00168: Tryptophan synthase beta chain pyridoxal-phosphate attachment site
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00291 PALP, Pyridoxal-phosphate dependent enzyme. 57 383 8e-76 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region CGCCCTCGCTGGTGGACAGTTGCAGGCGCAGGTCGGGGAGGGCACGGTTGAGCTGGTCCAGCCGCGGGATGAACCAGCGCGCCAGCAGGCTGCCGGGTAC GCCGAGGACGAACGGGGCCTCGGCGGTCTGCCGGCGCAGCTCGGCACAGACGCCACGCAGTCGCTCGAACGCATCGCCGCAGGCGTCACGCAGGCGAACG CCGGAATCGGTGAGTTTTACGCCGCGCCCATCCTTGCCGAACAGGGCCACCCCGAGATCGTCCTCGAGCAACCGCACCTGCCGGCTCACGGCGCCATGGG TAACGTGCAGTTCCTCGGCCGCCAGGCTGATGCTGTGCAACCGGGCAGCGGCTTCGAAAGCGCGCAGGGCATTCAGGGAGGGCAGGTCGCGGCTCATGGC TATTCGTGAGTTTTCCTGACAGGTTGCCGCAATCTTATCGGTTTCTTTTACCCGGCGGGGGGATTAGATTCTGCCCATACGTCATTTCCTGGAGCCCTCC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGACTTCCTATCGCAACGGCCCCGACGCCAAGGGCCTGTTCGGCCGCTTCGGCGGCCAGTACGTCGCCGAGACCCTGATGCCGCTGATCCTCGACCTCG CCCGCGAGTACGAGAAGGCCAAGGACGACCCGGCGTTCCAGGAGGAACTGGCCTACTTCCAGCGCGACTACGTCGGTCGACCGAGCCCGCTGTACTTCGC CGAGCGCCTGACCGAGCACTGCGGCGGGGCGAAGATCTACCTCAAGCGCGAGGAGCTGAACCATACCGGCGCGCACAAGATCAACAACTGCATCGGCCAG ATCCTCCTGGCCCGGCGCATGGGCAAGAAACGCATCATCGCCGAGACCGGCGCCGGCATGCACGGCGTGGCCACTGCCACCGTCGCCGCGCGCTTCGGCC TGCAGTGCGTGATCTACATGGGCACCACCGACATCGACCGGCAGCAGGCCAACGTCTTCCGCATGAAGCTGCTGGGCGCCGAGGTGATCCCGGTGACCGC CGGCACCGGTACCCTGAAGGACGCCATGAACGAGGCGCTGCGCGACTGGGTGACCAACGTCGACAGCACCTTCTACCTGATCGGCACGGTCGCCGGCCCG CATCCGTACCCGGCGATGGTCCGCGACTTCCAGGCGGTGATCGGCAAGGAAACCCGCGAGCAACTGGCCGAGAAGGAAGGGCGCCTGCCCGATTCGCTGG TCGCCTGCATCGGCGGCGGCTCCAACGCCATGGGCCTGTTCCACCCGTTCCTCGACGACGCCGGGGTGCAGATCGTCGGCGTGGAAGCCGCCGGCCACGG CATCGACACCGGCAAGCACGCGGCCAGCCTGAACGGCGGGGTTCCCGGCGTGCTGCACGGCAACCGCACCTTCCTGCTGCAGGACGCGGATGGCCAGATC ATCGACGCACACTCCATCTCCGCCGGCCTCGACTATCCCGGCATCGGCCCGGAACACGCCTGGCTGCACGACACCGGCCGCGTCGAGTACACCTCGATCA CCGACGACGAAGCCCTGGAGGCCTTCCACACCTGCTGCCGCCTCGAAGGCATCATCCCGGCACTGGAAAGCTCCCATGCCCTGGCCGAGGTCTTCAAGCG TGCGCCCAGCCTGCCCAAGGAGCACATCATGGTGGTGAACCTGTCCGGTCGCGGCGACAAGGACATGCAGACCGTCATGCACCACATGCAACAGGAGTCG AAAGCATGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MTSYRNGPDAKGLFGRFGGQYVAETLMPLILDLAREYEKAKDDPAFQEELAYFQRDYVGRPSPLYFAERLTEHCGGAKIYLKREELNHTGAHKINNCIGQ ILLARRMGKKRIIAETGAGMHGVATATVAARFGLQCVIYMGTTDIDRQQANVFRMKLLGAEVIPVTAGTGTLKDAMNEALRDWVTNVDSTFYLIGTVAGP HPYPAMVRDFQAVIGKETREQLAEKEGRLPDSLVACIGGGSNAMGLFHPFLDDAGVQIVGVEAAGHGIDTGKHAASLNGGVPGVLHGNRTFLLQDADGQI IDAHSISAGLDYPGIGPEHAWLHDTGRVEYTSITDDEALEAFHTCCRLEGIIPALESSHALAEVFKRAPSLPKEHIMVVNLSGRGDKDMQTVMHHMQQES KA

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0036 gene of Pseudomonas aeruginosa PAO1, Chromosome (37890 to 39098 bp)


Displaying the first five SNPs out of a total of 84 found in all isolate strains ( View all 84 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA7 Transversion g.37899C>G p.Ser400His
 Missense
TCG(Ser) -> CAC(His) 		SNP-506743 View
Pseudomonas aeruginosa PA7 Transversion g.37900G>T p.Ser400His
 Missense
TCG(Ser) -> CAC(His) 		SNP-506744 View
Pseudomonas aeruginosa PA7 Transition g.37901A>G p.Ser400His
 Missense
TCG(Ser) -> CAC(His) 		SNP-506745 View
Pseudomonas aeruginosa PA7 Transversion g.37977C>G p.Glu374Asp
 Missense
GAG(Glu) -> GAC(Asp) 		SNP-506746 View
Pseudomonas aeruginosa PA7 Transition g.37981T>C p.Lys373Gly
 Missense
AAG(Lys) -> GGG(Gly) 		SNP-506747 View
Select to view SNPs in a specific strain
SNP Map
Operons/Regulons
Type operon
Genes
GeneProduct
trpA tryptophan synthase alpha chain
trpB tryptophan synthase beta chain
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13113
Genomic Context
Genomic context Explanation of Genomic Context Field - trpABI (Pseudomonas aeruginosa)
PubMed Nucleotide sequence of the genes for tryptophan synthase in Pseudomonas aeruginosa.
Hadero A, Crawford IP.
Mol Biol Evol. 1986 May;3(3):191-204.
PMID: 3127651
PSORTb 3.0: Improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes.
Yu NY, Wagner JR, Laird MR, Melli G, Rey S, Lo R, Dao P, Sahinalp SC, Ester M, Foster LJ, Brinkman FS.
Bioinformatics. 2010 May 13; [Epub ahead of print]
PMID: 20472543
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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