Pseudomonas Genome Database - Annotation for PA0130

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PA0130

Key to diagram

View in GBrowse:

18 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Operons/Regulons
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name		Alternate Gene Names
Locus tag	PA0130 (View 6 updates) Submit an Update	GenBank Identifier (GI)	15595328 (Precomputed BLAST results at BLINK )
Genomic location	149054 - 147561 (-)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG000128 Orthology uncertain because more than one top BLASTp hit was identified.
Product Information
Product Name	probable aldehyde dehydrogenase (Class 3 )
Alternate Product Names
Subcellular localization	Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA00131
Systomonas	PA0130
RefSeq	NP_248820.1
GI	15595328
KEGG	PA0130
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Operons/Regulons

Type

operon

Genes

Gene	Product
PA0130	probable aldehyde dehydrogenase
PA0131	hypothetical protein
PA0132	beta-alanine--pyruvate transaminase

Evidence

Computationally Predicted

PubMed

18988623

Cross-Reference

DOOR-13131

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Putative enzymes

GO Accession

GO Term

Evidence

Molecular Function

GO:0004491

methylmalonate-semialdehyde dehydrogenase (acylating) activity

IEA

Biological Process

GO:0006574

valine catabolic process

IEA

Cellular Component

COG Prediction

Accession	Description	Category
COG1012	PutA, NAD-dependent aldehyde dehydrogenases [Energy production and conversion].	Energy production and conversion

Pathways

Name	Database	Xref
Valine, leucine and isoleucine degradation	KEGG	pae00280	Computationally Generated
Inositol phosphate metabolism	KEGG	pae00562	Computationally Generated
Propanoate metabolism	KEGG	pae00640	Computationally Generated
Metabolic pathways	KEGG	pae01100	Computationally Generated

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

68% similar to methylmalonate semialdehyde dehydrogenase MmsA [Pseudomonas aeruginosa]

Most Similar Human Homolog

ENSP00000342564, Percent Identity: 47.51%, E-Value: 5e-133, Ensembl Release: 56.
Description: Methylmalonate-semialdehyde dehydrogenase

Structure Features

PS00687: Aldehyde dehydrogenases glutamic acid active site

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF00171	Aldedh, Aldehyde dehydrogenase family.	13	477	1e-137	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

CTGGCAACAGGGTTTCTACGTGCGCTTCGGCGGCGATACCCTGCAATTCGGCCCGACCTTCAACGCCAGGCCGGAAGAGCTGGACCGCCTGTTCGACGCG GTCGGCGAAGCGCTCAACGGCATCGCCTGATGAGCGGCCGCCCGCAAGCCGTACCCACCGTGCAGGTGGACAACGCCGAGGTGATCGTGACCGAATGGCG CTTCGCCCCCGGCGCGGAAACCGGGCGCCACCGCCACGGCCACGACTATGTGGTGGTACCGCTGACCGACGGCACGCTGCTGCTGGAAACCCCCGAGGGC GACCGCCACGCGCCGCTGGTCGCCGGACAGGCCTACTTCCGCAAGGCCGGCGTCGAGCACAACGTGATCAACGCCAGCGCACACGAAGTGGTCTTCGTCG AGACCGAGATCAAGTAATCCGCACCCGCTCCCCAGGGAGCGGGTAAAGGGAAAGGGCCACGGCCCTTTCGTCACCCCTAGCGAAGAGAGAACAAGACAAG

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGGGCACCCTCCACCACCTGATCAACGGCGAAATGGTCGCCGACAACGGCCGCAGCGCCGACGTCTTCAACCCCTCCACCGGCGAAGCCATCCACAAGG TCCCGCTGGCCGACGGCAAGACCCTGCAGAAAGCCATCGATGCCGCCAGGGCCGCTTTCCCGGCCTGGCGCAACACCCCGCCGGCCAAGCGTGCACAGGT GCTGTACCGCTTCAAGCAACTGCTGGAGCAGAACGAGGCGCGGATTTCCAAGCTGATCAGCGAAGAGCACGGCAAGACCCTGGAAGACGCCGCCGGCGAA CTCAAGCGCGGCATCGAGAACGTCGAGTACGCCTGCGCCGCCCCGGAAATCCTCAAGGGCGAGTACAGCCGCAACGTCGGCCCGAACATCGACGCCTGGA GCGACTTCCAGCCGATCGGCGTGGTCGCCGGGATCACCCCGTTCAACTTCCCGGCGATGGTGCCGCTGTGGATGTACCCGCTGGCCATCGCCTGCGGCAA CACCTTCATCCTCAAGCCCTCCGAGCGCGACCCCAGCTCCACCCTGCTGATCGCCGAGCTGTTCCACGAAGCCGGCCTGCCGAAGGGCGTGCTGAACGTG GTGCACGGCGACAAGGAAGCCGTGGACGGCTTGCTCCAGGCGCCGGAAGTGAAAGCCATCAGCTTCGTCGGCTCGACCCCCATCGCCGAATACATCTATG CCGAGGGCACCAAGCGCGGCAAGCGCGTGCAGGCCCTGGGCGGCGCGAAGAACCATGCGGTGCTGATGCCCGACGCCGACCTCGACAACGCCGTCAGCGC GTTGATGGGTGCCGCCTACGGCTCCTGCGGCGAGCGCTGCATGGCGATCTCGGTGGCGGTATGCGTCGGCGACCAGGTGGCCGACGCGCTGATCGCCAAG CTGGTTCCGCAGATCAAGGCGCTGAAGATCGGCGCCGGCACTTCCTGCGGCCTCGACATGGGCCCGCTGGTCACTGCCGCCGCGCAGGCCAAGGTCACCG GCTACATCGACAGCGGCGTGGCCCAGGGCGCGGAGCTGGTGGTGGACGGCCGCGGCTACCAGGTCGCCGGCCACGAGAACGGCTTCTTCCTCGGCGGCAG CCTGTTCGACCGGGTGACCCCGGAAATGACCATCTACAAGGAAGAGATCTTCGGCCCGGTGCTGTGCGTGGTTCGCGTCAACAGCCTGGAAGAGGCCATG CAGTTGATCAACGACCACGAATACGGCAACGGCACCTGCATCTTCACCCGCGACGGCGAGGCCGCACGCCTGTTCTGCGACGAGATCGAGGTCGGCATGG TCGGCGTCAACGTCCCGCTGCCGGTGCCGGTGGCCTACCACAGCTTCGGCGGCTGGAAGCGCTCGCTGTTCGGCGACCTCCACGCCTACGGCCCGGACGG CGTGCGCTTCTACACCCGGCGCAAGGCCATCACCCAGCGCTGGCCGCAGCGGGCCAGCCACGAGGCCTCGCAATTCGCCTTCCCCAGCCTCTGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MGTLHHLINGEMVADNGRSADVFNPSTGEAIHKVPLADGKTLQKAIDAARAAFPAWRNTPPAKRAQVLYRFKQLLEQNEARISKLISEEHGKTLEDAAGE LKRGIENVEYACAAPEILKGEYSRNVGPNIDAWSDFQPIGVVAGITPFNFPAMVPLWMYPLAIACGNTFILKPSERDPSSTLLIAELFHEAGLPKGVLNV VHGDKEAVDGLLQAPEVKAISFVGSTPIAEYIYAEGTKRGKRVQALGGAKNHAVLMPDADLDNAVSALMGAAYGSCGERCMAISVAVCVGDQVADALIAK LVPQIKALKIGAGTSCGLDMGPLVTAAAQAKVTGYIDSGVAQGAELVVDGRGYQVAGHENGFFLGGSLFDRVTPEMTIYKEEIFGPVLCVVRVNSLEEAM QLINDHEYGNGTCIFTRDGEAARLFCDEIEVGMVGVNVPLPVPVAYHSFGGWKRSLFGDLHAYGPDGVRFYTRRKAITQRWPQRASHEASQFAFPSL

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0130 gene of Pseudomonas aeruginosa PAO1, Chromosome (147561 to 149054 bp)

Displaying the first five SNPs out of a total of 50 found in all isolate strains ( View all 50 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa PA7	Transition	g.147657G>A		Silent GAC(Asp) -> GAT(Asp)	SNP-409570	View
Pseudomonas aeruginosa PA14	Transition	g.147723C>T		Silent GTG(Val) -> GTA(Val)	SNP-135867	View
Pseudomonas aeruginosa PA14	Transversion	g.147789T>G		Silent GCA(Ala) -> GCC(Ala)	SNP-135868	View
Pseudomonas aeruginosa PA7	Transversion	g.147789T>G		Silent GCA(Ala) -> GCC(Ala)	SNP-409571	View
Pseudomonas aeruginosa PA7	Transition	g.147798G>A		Silent GGC(Gly) -> GGT(Gly)	SNP-409572	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Characterization of the mmsAB operon of Pseudomonas aeruginosa PAO encoding methylmalonate-semialdehyde dehydrogenase and 3-hydroxyisobutyrate dehydrogenase.
Steele MI, Lorenz D, Hatter K, Park A, Sokatch JR.
J Biol Chem. 1992 Jul 5;267(19):13585-92.
PMID: 1339433