Pseudomonas Genome Database - Annotation for PA0154

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PA0154

Key to diagram

View in GBrowse:

15 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Operons/Regulons
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name	pcaG	Alternate Gene Names
Locus tag	PA0154 (View 7 updates) Submit an Update	GenBank Identifier (GI)	15595352 (Precomputed BLAST results at BLINK )
Genomic location	175503 - 176108 (+)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG000152
Product Information
Product Name	protocatechuate 3,4-dioxygenase, alpha subunit (Class 2 )
Alternate Product Names
Subcellular localization	Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA00155
Systomonas	PA0154
RefSeq	NP_248844.1
GI	15595352
KEGG	PA0154
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Operons/Regulons

Type

operon

Genes

Gene	Product
pcaH	protocatechuate 3,4-dioxygenase, beta subunit
pcaG	protocatechuate 3,4-dioxygenase, alpha subunit

Evidence

Computationally Predicted

PubMed

18988623

Cross-Reference

DOOR-13135

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Carbon compound catabolism

GO Accession

GO Term

Evidence

Molecular Function

GO:0018578

protocatechuate 3,4-dioxygenase activity

IEA

Biological Process

GO:0044248	cellular catabolic process	ISS
GO:0015978	carbon utilization by utilization of organic compounds	ISS
GO:0046278	protocatechuate metabolic process	IEA

Cellular Component

COG Prediction

Accession	Description	Category
COG3485	PcaH, Protocatechuate 3,4-dioxygenase beta subunit [Secondary metabolites biosynthesis, transport, and catabolism].	Secondary metabolites biosynthesis, transport, and catabolism

Pathways

Name	Database	Xref
Aromatic compound catabolism	PseudoCAP
protocatechuate degradation, ortho-cleavage	PseudoCyc	PROTOCATECHUATE-ORTHO-CLEAVAGE-PWY
gallate degradation I	PseudoCyc	GALLATE-DEGRADATION-I-PWY
Benzoate degradation via hydroxylation	KEGG	pae00362	Computationally Generated
2,4-Dichlorobenzoate degradation	KEGG	pae00623	Computationally Generated
Metabolic pathways	KEGG	pae01100	Computationally Generated

Reactions

EC Number

1.13.11.3

Enzyme Assays

PDF file(s) provided by Sigma-Aldrich:
Protocatechuate 3,4-Dioxygenase (1.13.11.3)

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

87% similar to protocatechuate 3,4-dioxygenase, alpha subunit [Pseudomonas putida]

Structure Features

PS00083: Intradiol ring-cleavage dioxygenases proteins

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF00775	Dioxygenase_C, Dioxygenase.	42	147	2e-10	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

GCGCATCATCATGTTCGGCCGGGTCTTCGACCAGTACGGCAAGCCGGTCCCGCACACCCTGGTGGAGATGTGGCAGGCCAACGCCGGCGGGCGCTACCGG CACAAGAACGACCGCTACCTGGCGCCGCTCGACCCGAACTTCGGCGGCGTCGGCCGGACCCTCACCGACAGCGAGGGCCACTATTACTTCCGCACCATCA AGCCCGGGCCCTATCCCTGGCGCAACGGCCCCAACGACTGGCGCCCGGCGCACATCCACGTGTCTGTCAGCGGCCCGGCGATCGCCGCGCGGCTGGTCAC CCAGATGTACTTCGAGGGCGACCCGCTGATCCCCAAGTGCCCGATCATCCGCACCCTTGCCGACCCGGAGGCGGCGCAGAGCCTGATCGGCCGGCTCGAC ATGAGCATGGCCAACCCGATGGATTGCCTGGCCTACCGCTTCGACATCGTCCTGCGCGGGCAGCGTAAGACCTTCTTCGAGAACGCCTGAGGAGCCGACC

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGAGCCACGAACTGCTGCGGGAAACCCCTTCCCAGACCGCCGGTCCCTATGTCCACATCGGCCTGGCGCTGGCCGCCGCCGGCAACCCGACCCGTGACC TGGAGATCTGGAACCGCATGGCCCGCGAAGGCGCGCGGGGCCAGCACATCCTCCTGCTCGGCCACGTCTACGACGGCAACGGCCACCTGGTGCGCGACTC GTTCCTGGAGTTCTGGCAGGCCGACAGCGAAGGCCGCTACCAGGAGCGCTACGATCCGGAGCGTCCGTTCAACAGCTTCGGCCGCACCGCCACCACCTTC GATGCCGGCGAGTGGACCCTGCACACGGTCAAGCCGGGGGTGGTGCGTAACGCCCAGGGCGTGCCGATGGCGCCGCACATCAACGTCTCGCTGTTCGCCC GCGGCATCAACATCCACTTGCAGACCCGGCTGTACTTCGATGACGAGACCGAGGCCAACGCCGCCTGTCCGGTGCTCAACCTGATCGAGCAGGCGCCGCG TCGCGACACCCTGCTCGCGCAGCGCTGCGAAGTGGACGGACAGCTCGCCTACCGTTTCGACATCCGCATCCAGGGCGACAACGAGACGGTGTTCTTCGAC TTCTAG

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MSHELLRETPSQTAGPYVHIGLALAAAGNPTRDLEIWNRMAREGARGQHILLLGHVYDGNGHLVRDSFLEFWQADSEGRYQERYDPERPFNSFGRTATTF DAGEWTLHTVKPGVVRNAQGVPMAPHINVSLFARGINIHLQTRLYFDDETEANAACPVLNLIEQAPRRDTLLAQRCEVDGQLAYRFDIRIQGDNETVFFD F

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0154 gene of Pseudomonas aeruginosa PAO1, Chromosome (175503 to 176108 bp)

Displaying the first five SNPs out of a total of 66 found in all isolate strains ( View all 66 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa PA7	Transition	g.175547T>C		Silent GGT(Gly) -> GGC(Gly)	SNP-242304	View
Pseudomonas aeruginosa PA14	Transition	g.175553T>C		Silent TAT(Tyr) -> TAC(Tyr)	SNP-133020	View
Pseudomonas aeruginosa PA7	Transition	g.175553T>C		Silent TAT(Tyr) -> TAC(Tyr)	SNP-242305	View
Pseudomonas aeruginosa PA14	Transition	g.175598T>C		Silent CGT(Arg) -> CGC(Arg)	SNP-133021	View
Pseudomonas aeruginosa PA7	Transition	g.175598T>C		Silent CGT(Arg) -> CGC(Arg)	SNP-242306	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Crystal structures of substrate and substrate analog complexes of protocatechuate 3,4-dioxygenase: endogenous Fe3+ ligand displacement in response to substrate binding.
Orville AM, Lipscomb JD, Ohlendorf DH.
Biochemistry. 1997 Aug 19;36(33):10052-66.
PMID: 9254600