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               PA0230               
Key to diagram


View in GBrowse: 15 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name pcaB  Alternate Gene Names
Locus tag PA0230 (View 6 updates) Submit an Update GenBank Identifier (GI) 15595427 (Precomputed BLAST results at BLINK )
Genomic location 259784 - 261163 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name 3-carboxy-cis,cis-muconate cycloisomerase (Class 2  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA00231
Systomonas PA0230
RefSeq NP_248921.1
GI 15595427
KEGG PA0230
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
pcaT dicarboxylic acid transporter PcaT
pcaB 3-carboxy-cis,cis-muconate cycloisomerase
pcaD beta-ketoadipate enol-lactone hydrolase
pcaC gamma-carboxymuconolactone decarboxylase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13151
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Carbon compound catabolism
GO Accession GO Term Evidence
Molecular Function
GO:0018842 3-carboxymuconate cycloisomerase type II activity RCA
GO:0047472 3-carboxy-cis,cis-muconate cycloisomerase activity IEA
Biological Process
GO:0044248 cellular catabolic process ISS
GO:0015978 carbon utilization by utilization of organic compounds ISS
GO:0009712 catechol metabolic process RCA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0015 PurB, Adenylosuccinate lyase [Nucleotide transport and metabolism]. Nucleotide transport and metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Aromatic compound catabolism PseudoCAP
protocatechuate degradation, ortho-cleavage PseudoCyc PROTOCATECHUATE-ORTHO-CLEAVAGE-PWY
Benzoate degradation via hydroxylation KEGG pae00362 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 5.5.1.2
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 87% similar to 3-carboxy-cis,cis-muconate cycloisomerase [Pseudomonas putida]
Most Similar Human Homolog
ENSP00000216194, Percent Identity: 26.89%, E-Value: 5e-08, Ensembl Release: 56.
Description: Adenylosuccinate lyase
Structure Features
Structure Features Explanation of Structure Features Field BL00163: Fumarate lyases proteins
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00206 Lyase_1, Lyase. 16 304 4e-61 23.0
PF10397 ADSL_C, Adenylosuccinate lyase C-terminus. 367 446 1e-20 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region CAAGACCGCCAGCGCGGTGATGACCTTCGCCCTGCTGGCGTACATGCTGATGCAACCGCTGTTCGGCATGCTTTCCGATCGCATCGGGCGCAAGACCTCG ATGCTCTGGTTCGGCACCCTGGGCACGCTCTGCACCCTGCCGATCCTGCTCGCGCTGAAGACCGTGAGCAGTCCCTACATGGCCTTCTTCCTGATCATCC TGGCGCTGGCCATCGTCAGCCTGTACACCTCCATCAGCGGCCTGATCAAGGCCGAGATGTTCCCGCCGGAGGTGCGCGCCCTCGGCGTCGGAGTGTCCTA CGCGGTGGGCAACGCGATCTTCGGCGGCTCGGCCGAGTACGTCGCCCTGGGGCTGAAGTCGGTAGGGATGGAGAACGTCTTCTACTGGTATGTTTCAGCC ATGTGCCTGCTGGCGCTGCTGGTCTGCGTGAGCCTGCCGGACCTGCGCAAGGTCAGCTACCTGAACGACGAGCCCTGACGGGCCGGGAAAAGGAAAACCG

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGAGCGACGTACCCGCCAACCAGTTGTTCGATGCCTATTTCATGGCGGCGCCGATGCGCGCGGTGTTCTCCGACCGCGGCCGCCTGCAAGGCATGCTCG ACTTCGAGGCCGCCCTGGCCCGCGCCGAGGCGCGCACCGGGGTGGTTCCCGCGACGGCGGTGGCGCCGATCGAGGCGGCCTGCCGCGCCGAACTGTACGA CCCGCTGGCGCTGGCCGAGGCGGTCGCCACTGCCGGCAACTCGGCGATCCCGCTGGTCAAGGCGCTGGGCCGCCAGGTGGCGGCTGGCGACGCCGAGGCC GAGCGCTACGTGCATCTCGGCGCCACCAGCCAGGACGCCATGGACAGCGGCCTGGTGCTGCAACTGCGCCGTGCGCTGGCCTTGCTCGAGCAGGATCTGC AACGTCTTGCCGAGGTCCTGGCGGACCAGGCCGAACGGCATGCCGACACGCCGCTGGCCGGGCGCACCTGGTTGCAGCACGCCACCCCGGTGACCCTGGG CATGAAGCTCGCCGGCCTGCTCGGTGCCTTGACCCGGCACCGCCAGCGCCTGCGCGAGTTGCGCCCGCGCCTGCTGGTGCTGCAATTCGGCGGCGCCTCG GGGACGCTCGCCGCGCTCGGCGAACAGGCCCTGCCGGTGGCCGCGGCGCTGGCCGAGGAGCTGGGCCTGGCTTTGCCCGAACAACCCTGGCACACCCAGC GCGACCGCCTGGTGGAATTCGCCAGCGTGCTCGGCCTGGTGGCCGGTAGCCTCGGCAAGTTCGGTCGCGATGTCAGCCTGCTGATGCAGACCGAGGCCGG CGAGGTCTTCGAGCCGGCCGGCGCCGGGCGCGGCGGTTCCTCGACCATGCCGCACAAGCGCAACCCGGTGAGCAGCGCGGTGCTGATCGCCGCCGCCACC CGTGCGCCGGGGCTGGTCTCGACCCTGTTCGCGGCGATGCCGCAGGAACACGAGCGCAGCCTCGGTCTCTGGCACGCCGAGTGGGAAACCCTGCCGGAAC TCTGTTGCCTGGTTGCCGGCGCGCTGCAACAGGCGATCGGCCTGCTCGAAGGCCTGGAGGTGGACGCCCAGCGGATGCGCCGCAACCTTGGCCTGACCCA CGGCCTGGTGCTCGCCGAGGCGGTGAGCATCGCCCTGGCCCGCCGTATCGGCCGCGAAGCCGCGCACCATCTGGTGGAACAGTGCTGCCGCCGCGCCGTC GAGCAGCGCCGCGAGTTGCGCGCGGTGCTCGGCGAAGAGGCGCGGGTGAGCGCCGAGTTGTCCGGCGACGAACTGGACCGCCTGCTCGATCCCGCCCATT ACCTCGGCCAGGCCCGCGCCTGGGTCGAGCGCGCGCTCGCCGAACACCATGCCCTGGGCTTCGAACCCCATCCCGCCTGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MSDVPANQLFDAYFMAAPMRAVFSDRGRLQGMLDFEAALARAEARTGVVPATAVAPIEAACRAELYDPLALAEAVATAGNSAIPLVKALGRQVAAGDAEA ERYVHLGATSQDAMDSGLVLQLRRALALLEQDLQRLAEVLADQAERHADTPLAGRTWLQHATPVTLGMKLAGLLGALTRHRQRLRELRPRLLVLQFGGAS GTLAALGEQALPVAAALAEELGLALPEQPWHTQRDRLVEFASVLGLVAGSLGKFGRDVSLLMQTEAGEVFEPAGAGRGGSSTMPHKRNPVSSAVLIAAAT RAPGLVSTLFAAMPQEHERSLGLWHAEWETLPELCCLVAGALQQAIGLLEGLEVDAQRMRRNLGLTHGLVLAEAVSIALARRIGREAAHHLVEQCCRRAV EQRRELRAVLGEEARVSAELSGDELDRLLDPAHYLGQARAWVERALAEHHALGFEPHPA

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0230 gene of Pseudomonas aeruginosa PAO1, Chromosome (259784 to 261163 bp)


Displaying the first five SNPs out of a total of 121 found in all isolate strains ( View all 121 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA7 Transition g.259816T>C
Silent
GAT(Asp) -> GAC(Asp) 		SNP-222493 View
Pseudomonas aeruginosa PA7 Transition g.259822T>C
Silent
TAT(Tyr) -> TAC(Tyr) 		SNP-222494 View
Pseudomonas aeruginosa C3719 Transition g.259827T>C
Not determined
ATG(Met) -> ACG() 		SNP-12387 View
Pseudomonas aeruginosa PACS2 Transition g.259827T>C p.Met15Thr
 Missense
ATG(Met) -> ACG(Thr) 		SNP-126586 View
Pseudomonas aeruginosa PA14 Transition g.259827T>C p.Met15Thr
 Missense
ATG(Met) -> ACG(Thr) 		SNP-163358 View
Select to view SNPs in a specific strain
SNP Map
PubMed 3-Carboxy-cis,cis-muconate lactonizing enzyme from Pseudomonas putida is homologous to the class II fumarase family: a new reaction in the evolution of a mechanistic motif.
Williams SE, Woolridge EM, Ransom SC, Landro JA, Babbitt PC, Kozarich JW.
Biochemistry. 1992 Oct 13;31(40):9768-76.
PMID: 1390752
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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