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               PA0231               
Key to diagram


View in GBrowse: 16 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name pcaD  Alternate Gene Names
Locus tag PA0231 (View 6 updates) Submit an Update GenBank Identifier (GI) 15595428 (Precomputed BLAST results at BLINK )
Genomic location 261179 - 261970 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name beta-ketoadipate enol-lactone hydrolase (Class 2  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Unknown (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA00232
Systomonas PA0231
RefSeq NP_248922.1
GI 15595428
KEGG PA0231
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
pcaT dicarboxylic acid transporter PcaT
pcaB 3-carboxy-cis,cis-muconate cycloisomerase
pcaD beta-ketoadipate enol-lactone hydrolase
pcaC gamma-carboxymuconolactone decarboxylase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13151
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Carbon compound catabolism
GO Accession GO Term Evidence
Molecular Function
GO:0016788 hydrolase activity, acting on ester bonds RCA
GO:0047570 3-oxoadipate enol-lactonase activity IEA
Biological Process
GO:0044248 cellular catabolic process ISS
GO:0015978 carbon utilization by utilization of organic compounds ISS
GO:0019614 catechol catabolic process RCA
GO:0042952 beta-ketoadipate pathway IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0596 MhpC, Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) [General function prediction only]. General function prediction only
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Aromatic compound catabolism PseudoCAP
protocatechuate degradation, ortho-cleavage PseudoCyc PROTOCATECHUATE-ORTHO-CLEAVAGE-PWY
catechol degradation, ortho-cleavage PseudoCyc CATECHOL-ORTHO-CLEAVAGE-PWY
Benzoate degradation via hydroxylation KEGG pae00362 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 3.1.1.24
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 63% similar to B-ketoadipate enol-lactone hydrolase [Bradyrhizobium japonicum]
Structure Features
Structure Features Explanation of Structure Features Field PR00412: Epoxide hydrolase signature
PR00111: Alpha/beta hydrolase fold signature
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00561 Abhydrolase_1, alpha/beta hydrolase fold. 48 258 2e-17 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region CCACCCGTGCGCCGGGGCTGGTCTCGACCCTGTTCGCGGCGATGCCGCAGGAACACGAGCGCAGCCTCGGTCTCTGGCACGCCGAGTGGGAAACCCTGCC GGAACTCTGTTGCCTGGTTGCCGGCGCGCTGCAACAGGCGATCGGCCTGCTCGAAGGCCTGGAGGTGGACGCCCAGCGGATGCGCCGCAACCTTGGCCTG ACCCACGGCCTGGTGCTCGCCGAGGCGGTGAGCATCGCCCTGGCCCGCCGTATCGGCCGCGAAGCCGCGCACCATCTGGTGGAACAGTGCTGCCGCCGCG CCGTCGAGCAGCGCCGCGAGTTGCGCGCGGTGCTCGGCGAAGAGGCGCGGGTGAGCGCCGAGTTGTCCGGCGACGAACTGGACCGCCTGCTCGATCCCGC CCATTACCTCGGCCAGGCCCGCGCCTGGGTCGAGCGCGCGCTCGCCGAACACCATGCCCTGGGCTTCGAACCCCATCCCGCCTGACTGGAGATCCCCTTC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence GTGCCAACCGTACGCCTCGCCGACGGCGACCTGAACTACTCCCTGGAAGGACCGGCCGGCGCGCCGGTCCTGCTGTTGTCAAACTCGCTGGGCACCGACC TGGGCATGTGGGACACGCAGATCCCCGCACTCACCGCCCATTTCCGCGTGCTGCGCTACGACACCCGCGGCCACGGCGCCTCGCTGGTCACCCCCGGACC GTACGCCATCGGCCAGCTCGGCGCCGACGTGCTTGCCCTGCTCGATGCCCTCGAGCTGCCGCGCGTGCATTTCTGCGGGCTGTCCATGGGCGGCCTGATC GGCCAGTGGCTGGGCATCCACGCCGGCGAGCGGCTCGGCCGGCTGGTGCTGTGCAACACCGCGGCGAAGATTGCCAGCGACGAGGTCTGGAACACCCGCA TCGATACCGTCCTGAAAGGCGGCGAGCAGGCCATGCGTGACCTGCGCGACGCCTCGGTGGCGCGCTGGTTCACCGCGGGCTTCGCCGAGCGCGAACCGGC GCAGGTCGAGCGCATCGTCGCGATGCTCGCGGCCACCTCGCCGCAGGGCTACGCGGCCAACTGCGCTGCGGTACGCGACGCCGATTTCCGCGAGCAGCTC GGGCTGGTCCAGGCGCCGACGCTGATCGTCGCCGGCAGCCACGATGCCGTGACCACTCCGGACAACGCCCGCTTCATGCAGGCGCGCATCGCCGACGCGC AGTTGGTCGAGTTCGCCGCCGCGCACCTGTCCAACGTCGAAGCGGGCGACGCCTTCAGCCGCCGCCTGGTCGATTTCCTGCTGTCCGCCTGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MPTVRLADGDLNYSLEGPAGAPVLLLSNSLGTDLGMWDTQIPALTAHFRVLRYDTRGHGASLVTPGPYAIGQLGADVLALLDALELPRVHFCGLSMGGLI GQWLGIHAGERLGRLVLCNTAAKIASDEVWNTRIDTVLKGGEQAMRDLRDASVARWFTAGFAEREPAQVERIVAMLAATSPQGYAANCAAVRDADFREQL GLVQAPTLIVAGSHDAVTTPDNARFMQARIADAQLVEFAAAHLSNVEAGDAFSRRLVDFLLSA

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0231 gene of Pseudomonas aeruginosa PAO1, Chromosome (261179 to 261970 bp)


Displaying the first five SNPs out of a total of 46 found in all isolate strains ( View all 46 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA7 Transversion g.261247C>G
Silent
GTC(Val) -> GTG(Val) 		SNP-222554 View
Pseudomonas aeruginosa PA14 Transition g.261319T>C
Silent
CAT(His) -> CAC(His) 		SNP-163369 View
Pseudomonas aeruginosa PA7 Transition g.261319T>C
Silent
CAT(His) -> CAC(His) 		SNP-222555 View
Pseudomonas aeruginosa PA7 Transition g.261346C>T
Silent
CGC(Arg) -> CGT(Arg) 		SNP-222556 View
Pseudomonas aeruginosa PA7 Transition g.261383G>A p.Ala69Thr
 Missense
GCC(Ala) -> ACC(Thr) 		SNP-222557 View
Select to view SNPs in a specific strain
SNP Map
PubMed Characterization of Bradyrhizobium japonicum pcaBDC genes involved in 4-hydroxybenzoate degradation.
Lorite MJ, Sanjuan J, Velasco L, Olivares J, Bedmar EJ.
Biochim Biophys Acta. 1998 May 11;1397(3):257-61.
PMID: 9582432
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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