Pseudomonas Genome Database - Annotation for PA0232

Add this annotation to your clipboard

PA0232

Key to diagram

View in GBrowse:

15 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Operons/Regulons
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name	pcaC	Alternate Gene Names
Locus tag	PA0232 (View 6 updates) Submit an Update	GenBank Identifier (GI)	15595429 (Precomputed BLAST results at BLINK )
Genomic location	261981 - 262382 (+)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG000231
Product Information
Product Name	gamma-carboxymuconolactone decarboxylase (Class 2 )
Alternate Product Names
Subcellular localization	Unknown (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA00233
Systomonas	PA0232
RefSeq	NP_248923.1
GI	15595429
KEGG	PA0232
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Operons/Regulons

Type

operon

Genes

Gene	Product
pcaT	dicarboxylic acid transporter PcaT
pcaB	3-carboxy-cis,cis-muconate cycloisomerase
pcaD	beta-ketoadipate enol-lactone hydrolase
pcaC	gamma-carboxymuconolactone decarboxylase

Evidence

Computationally Predicted

PubMed

18988623

Cross-Reference

DOOR-13151

Genomic Context

Genomic context

- Cluster of genes in protocatechuate degradative pathway. Another cluster is about 100 kb away.

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Carbon compound catabolism

GO Accession

GO Term

Evidence

Molecular Function

GO:0016831

carboxy-lyase activity

RCA

Biological Process

GO:0044248	cellular catabolic process	ISS
GO:0015978	carbon utilization by utilization of organic compounds	ISS
GO:0019619	protocatechuate catabolic process	RCA

Cellular Component

COG Prediction

Accession	Description	Category
COG0599	COG0599, Uncharacterized homolog of gamma-carboxymuconolactone decarboxylase subunit [Function unknown].	Function unknown

Pathways

Name	Database	Xref
Aromatic compound catabolism	PseudoCAP
protocatechuate degradation, ortho-cleavage	PseudoCyc	PROTOCATECHUATE-ORTHO-CLEAVAGE-PWY
Benzoate degradation via hydroxylation	KEGG	pae00362	Computationally Generated
Metabolic pathways	KEGG	pae01100	Computationally Generated

Reactions

EC Number

4.1.1.44

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

78% similar to gamma-carboxymuconolactone decarboxylase [Acinetobacter sp. ADP1]

Structure Features

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF02627	CMD, Carboxymuconolactone decarboxylase family.	36	118	1e-18	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

CCAGTGGCTGGGCATCCACGCCGGCGAGCGGCTCGGCCGGCTGGTGCTGTGCAACACCGCGGCGAAGATTGCCAGCGACGAGGTCTGGAACACCCGCATC GATACCGTCCTGAAAGGCGGCGAGCAGGCCATGCGTGACCTGCGCGACGCCTCGGTGGCGCGCTGGTTCACCGCGGGCTTCGCCGAGCGCGAACCGGCGC AGGTCGAGCGCATCGTCGCGATGCTCGCGGCCACCTCGCCGCAGGGCTACGCGGCCAACTGCGCTGCGGTACGCGACGCCGATTTCCGCGAGCAGCTCGG GCTGGTCCAGGCGCCGACGCTGATCGTCGCCGGCAGCCACGATGCCGTGACCACTCCGGACAACGCCCGCTTCATGCAGGCGCGCATCGCCGACGCGCAG TTGGTCGAGTTCGCCGCCGCGCACCTGTCCAACGTCGAAGCGGGCGACGCCTTCAGCCGCCGCCTGGTCGATTTCCTGCTGTCCGCCTGAGGAGCCGCCG

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGGACGAGAAACAACGCTACGAAGCCGGCATGCAAGTGCGCCGCGCGGTGCTCGGCGCCGCCCATGTCGAGCGCAGCCTGGCCAACCTGACGCCGTTCA ACGAAGAGTTCCAGGAGATGATCACCCGCCACGCCTGGGGCGACATCTGGACCCGTCCGGGGCTGCCGCGACACACCCGCAGCCTGGTCACCATCGCCAT GCTGATCGGCATGAACCGCAACGAGGAACTCAAGCTGCATCTGCGCGCGGCGAAGAACAACGGGGTGAGCCGCGACGAGATCAAGGAAGTGATCATGCAG AGCGCCATCTACTGCGGTATTCCCGCGGCCAACGCGACCTTCCACCTGGCCGAATCGGTGTGGGACGAGCTTGGCGTGGAGTCGCAGCAGGCGTCCCGGT AG

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MDEKQRYEAGMQVRRAVLGAAHVERSLANLTPFNEEFQEMITRHAWGDIWTRPGLPRHTRSLVTIAMLIGMNRNEELKLHLRAAKNNGVSRDEIKEVIMQ SAIYCGIPAANATFHLAESVWDELGVESQQASR

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0232 gene of Pseudomonas aeruginosa PAO1, Chromosome (261981 to 262382 bp)

Displaying the first five SNPs out of a total of 35 found in all isolate strains ( View all 35 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa C3719	Transversion	g.262039C>A	p.Ala20Asp	Missense GCC(Ala) -> GAC(Asp)	SNP-9885	View
Pseudomonas aeruginosa 2192	Transversion	g.262039C>A	p.Ala20Asp	Missense GCC(Ala) -> GAC(Asp)	SNP-54040	View
Pseudomonas aeruginosa LESB58	Transversion	g.262039C>A	p.Ala20Asp	Missense GCC(Ala) -> GAC(Asp)	SNP-72629	View
Pseudomonas aeruginosa PACS2	Transversion	g.262039C>A	p.Ala20Asp	Missense GCC(Ala) -> GAC(Asp)	SNP-126590	View
Pseudomonas aeruginosa PA14	Transversion	g.262039C>A	p.Ala20Asp	Missense GCC(Ala) -> GAC(Asp)	SNP-163378	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Relationships among enzymes of the beta-ketoadipate pathway. 3. Properties of crystalline gamma-carboxymuconolactone decarboxylase from Pseudomonas putida.
Parke D, Meagher RB, Ornston LN.
Biochemistry. 1973 Aug 28;12(18):3537-42.
PMID: 4199896
Cloning and genetic organization of the pca gene cluster from Acinetobacter calcoaceticus.
Doten RC, Ngai KL, Mitchell DJ, Ornston LN.
J Bacteriol. 1987 Jul;169(7):3168-74.
PMID: 3036773