Pseudomonas Genome Database - Annotation for PA0244

Add this annotation to your clipboard

PA0244

Key to diagram

View in GBrowse:

14 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Operons/Regulons
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name		Alternate Gene Names
Locus tag	PA0244 (View 6 updates) Submit an Update	GenBank Identifier (GI)	15595441 (Precomputed BLAST results at BLINK )
Genomic location	277334 - 276480 (-)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG000242
Product Information
Product Name	hypothetical protein (Class 4 )
Alternate Product Names
Subcellular localization	Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA00245
Systomonas	PA0244
RefSeq	NP_248935.1
GI	15595441
KEGG	PA0244
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Operons/Regulons

Type

operon

Genes

Gene	Product
PA0244	hypothetical protein
aroQ2	3-dehydroquinate dehydratase

Evidence

Computationally Predicted

PubMed

18988623

Cross-Reference

DOOR-13154

Genomic Context

Genomic context

- Cluster of genes probably involved in carbon compound degradation

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Putative enzymes

GO Accession

GO Term

Evidence

Molecular Function

GO:0004764

shikimate 5-dehydrogenase activity

IEA

Biological Process

GO:0009423

chorismate biosynthetic process

IEA

Cellular Component

COG Prediction

Accession	Description	Category
COG0169	AroE, Shikimate 5-dehydrogenase [Amino acid transport and metabolism].	Amino acid transport and metabolism

Pathways

Name	Database	Xref
Phenylalanine, tyrosine and tryptophan biosynthesis	KEGG	pae00400	Computationally Generated
Metabolic pathways	KEGG	pae01100	Computationally Generated

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

49% similar to C terminal half of bifunctional dehydroquinate dehydratase/shikimate dehydrogenase [Nicotiana tabacum]
47% similar to shikimate 5-dehydrogenase [Escherichia coli]

Structure Features

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF08501	Shikimate_dh_N, Shikimate dehydrogenase substrate binding domain.	11	98	5e-21	23.0
PF01488	Shikimate_DH, Shikimate / quinate 5-dehydrogenase.	129	209	3e-07	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

TAACCGTTCGGTACATTTTCCCGGCATCGATGACATCATTGCTGTGGAGGCTGCGCCATGACGCGCACGGTACTGGTCCTGAACGGCCCCAACCTGAATC TGCTCGGCACCCGCGAGCCACAGACCTACGGCCGGCGGACCCTGGGCGAAATCGCCGACGAATGCGCGGCCTTCGCCGAGACGCGCGGCTTCGCCGTGGA CTTCCGCCAGACCAACCAGGAAGGCCAACTGCTCGACTGGATCCACCAGGCCCGCGGCCGGGTCGCCGGCATCGTCATCAACCCCGCCGCCTGGACCCAC ACCTCGGTGGCGCTGCGCGACGCCCTGGCCGCGGTGGAACTGCCGGTGGTCGAAGTGCACCTGTCCAACGTCCACCAGCGCGAAGCGTTCCGCCACCACT CCTACGTCTCGCCGGTGGCGCTGGGAGTGATCTGCGGATTCGGCAGCCTCGGCTACCGCCTGGCCCTGGAACATTTCGCCGAGCGTTTCGAGGCGGCCGC

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGAGCGCGCCGCGATCGGTTCTCGCCGGCCTGATCGGTGCCGGCATCCAGGCGTCGCGGACCCCTGCCCTGCACGAGCGCGAGGGCGACGCCCAGGGCA TCCGCTATCTGTACCGGCTGATCGATCTCGACCCGCTGGGCAAGTCGGCGGACGACCTGGAGTTCCTGCTCGCCGCCGCCAGCGACCTCGGCTTCACCGG GCTGAACGTCACCTTCCCCTGCAAGCAGGCGATCATCCCCCTGCTCGACGACCTCTCCGCCGAGGCACGCGGCATCGGCGCGGTGAACACCGTGGTGCTG CGCGATGGCCGGCGCATCGGCCACAACACCGACTGCCTGGGCTTCGCCGAGGGCTTTCGGCGCGGCCTGGCCGACGCGCCGCGCCAGCGCGTGGTGCAGA TGGGCGCGGGCGGCGCCGGCGCGGCAGTGGCCCATGCGCTGCTCGCCGAGGGCGTCGAGCGGCTGGTCCTGTTCGACGTCGACCCGGCGCGGGCCCAGGC CCTCGCGGACAACCTCAACCAGCACTTCGGCGCGCCACGAGCGCTCGCCGGCAGCGACCTGGCCGGCGCCCTGGGCGACGCCCAGGGATTGGTCAACACC ACCCCGGTGGGCATGGCCAAGCTACCCGGCATGCCGGTGCCGGCGGAACTGTTGCGCGCCGACCTGTGGGTCGCCGAGGTGATCTACTTTCCGCTGGAAA CCGAGCTGCTGCGCCAGGCCCGCGCCCTCGGCTGCCGCACACTGGACGGCGGCACCATGGCGGTGTTCCAGGCGGTGAAGGCATTCGAACTGTTCAGCGG GGTGACGGCCGACGCCGCGCGCATGCAGGCGCACTTCGCCAGCCTGGGCGACTGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MSAPRSVLAGLIGAGIQASRTPALHEREGDAQGIRYLYRLIDLDPLGKSADDLEFLLAAASDLGFTGLNVTFPCKQAIIPLLDDLSAEARGIGAVNTVVL RDGRRIGHNTDCLGFAEGFRRGLADAPRQRVVQMGAGGAGAAVAHALLAEGVERLVLFDVDPARAQALADNLNQHFGAPRALAGSDLAGALGDAQGLVNT TPVGMAKLPGMPVPAELLRADLWVAEVIYFPLETELLRQARALGCRTLDGGTMAVFQAVKAFELFSGVTADAARMQAHFASLGD

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0244 gene of Pseudomonas aeruginosa PAO1, Chromosome (276480 to 277334 bp)

Displaying the first five SNPs out of a total of 91 found in all isolate strains ( View all 91 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa PA7	Transversion	g.276489C>G		Silent CTG(Leu) -> CTC(Leu)	SNP-408415	View
Pseudomonas aeruginosa PA7	Transition	g.276492G>A		Silent AGC(Ser) -> AGT(Ser)	SNP-408416	View
Pseudomonas aeruginosa PA7	Transition	g.276501G>A		Silent CAC(His) -> CAT(His)	SNP-408417	View
Pseudomonas aeruginosa PA7	Transversion	g.276513G>C		Silent CGC(Arg) -> CGG(Arg)	SNP-408418	View
Pseudomonas aeruginosa PA7	Transversion	g.276519G>C		Silent GCC(Ala) -> GCG(Ala)	SNP-408419	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Cloning of cDNA encoding the bifunctional dehydroquinase.shikimate dehydrogenase of aromatic-amino-acid biosynthesis in Nicotiana tabacum.
Bonner CA, Jensen RA.
Biochem J. 1994 Aug 15;302 ( Pt 1):11-4.
PMID: 8067995