Pseudomonas Genome Database - Annotation for PA0245

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PA0245

Key to diagram

View in GBrowse:

11 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Operons/Regulons
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name	aroQ2	Alternate Gene Names	aroD2
Locus tag	PA0245 (View 6 updates) Submit an Update	GenBank Identifier (GI)	15595442 (Precomputed BLAST results at BLINK )
Genomic location	277777 - 277331 (-)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG000243
Product Information
Product Name	3-dehydroquinate dehydratase (Class 2 )
Alternate Product Names	dehydroquinase
Subcellular localization	Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA00246
Systomonas	PA0245
RefSeq	NP_248936.1
GI	15595442
KEGG	PA0245
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Operons/Regulons

Type

operon

Genes

Gene	Product
PA0244	hypothetical protein
aroQ2	3-dehydroquinate dehydratase

Evidence

Computationally Predicted

PubMed

18988623

Cross-Reference

DOOR-13154

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Amino acid biosynthesis and metabolism

GO Accession

GO Term

Evidence

Molecular Function

GO:0004334	fumarylacetoacetase activity	ISS
GO:0003855	3-dehydroquinate dehydratase activity	IEA

Biological Process

GO:0006520	amino acid metabolic process	ISS
GO:0009094	L-phenylalanine biosynthetic process	ISS
GO:0006571	tyrosine biosynthetic process	ISS
GO:0000162	tryptophan biosynthetic process	ISS
GO:0009423	chorismate biosynthetic process	IEA

Cellular Component

COG Prediction

Accession	Description	Category
COG0757	AroQ, 3-dehydroquinate dehydratase II [Amino acid transport and metabolism].	Amino acid transport and metabolism

Pathways

Name	Database	Xref
Phenylalanine, tyrosine and tryptophan biosynthesis	PseudoCAP
phenylalanine, tyrosine and tryptophan biosynthesis, complete	PseudoCyc	COMPLETE-ARO-PWY
chorismate biosynthesis	PseudoCyc	ARO-PWY
pathways of chorismate	PseudoCyc	ALL-CHORISMATE-PWY
Phenylalanine, tyrosine and tryptophan biosynthesis	KEGG	pae00400	Computationally Generated
Metabolic pathways	KEGG	pae01100	Computationally Generated

Reactions

EC Number

4.2.1.10

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

72% similar to putative catabolic dehydroquinase [Pseudomonas aeruginosa]
70% similar to 3-dehydroquinase AroQ [Actinobacillus pleuropneumoniae]

Structure Features

PS01029: Dehydroquinase class II signature

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF01220	DHquinase_II, Dehydroquinase class II.	5	144	4e-53	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

CAGTTCCCGACCAGCCTGGTGGCCGCGCAGAACGCCGTGGAGCAACGCCACATCGGCGTCGCCACCAGCAACGCGACGTTGTTCCGCTCCCTCGGCGGGG CAGTCGGGGTGGCGCTGATGTCGGCGATCCTGCTGGCCCTGCTCAAGCACCTGCGGATCGGCGGCGGGCTCGACCAGGGCTTTTCCGAGAGCGCCCTGCT GAGCAGCCTGGACGCCGCCAGCGGGCCGGCCCGGGTGATCCTCCAGCATGTCCTCGCGGATGCCTTCAAGGTGCTCTTCCTGGTCAACGCGGCGGTGGCG CTGCTCGGCCTGGCGGTGGCCTCGGCACTGCCCGACATGACCCTGCGCGGCCGCGGCGCGACGCAACCGACGGCAGGCGAGTGAGCGGACGCCGATAATC GAACGCTTTCGTCCCGTCGGCGACGGCGTCCGGTTTACAAAAGTAACCGTTCGGTACATTTTCCCGGCATCGATGACATCATTGCTGTGGAGGCTGCGCC

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGACGCGCACGGTACTGGTCCTGAACGGCCCCAACCTGAATCTGCTCGGCACCCGCGAGCCACAGACCTACGGCCGGCGGACCCTGGGCGAAATCGCCG ACGAATGCGCGGCCTTCGCCGAGACGCGCGGCTTCGCCGTGGACTTCCGCCAGACCAACCAGGAAGGCCAACTGCTCGACTGGATCCACCAGGCCCGCGG CCGGGTCGCCGGCATCGTCATCAACCCCGCCGCCTGGACCCACACCTCGGTGGCGCTGCGCGACGCCCTGGCCGCGGTGGAACTGCCGGTGGTCGAAGTG CACCTGTCCAACGTCCACCAGCGCGAAGCGTTCCGCCACCACTCCTACGTCTCGCCGGTGGCGCTGGGAGTGATCTGCGGATTCGGCAGCCTCGGCTACC GCCTGGCCCTGGAACATTTCGCCGAGCGTTTCGAGGCGGCCGCATGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MTRTVLVLNGPNLNLLGTREPQTYGRRTLGEIADECAAFAETRGFAVDFRQTNQEGQLLDWIHQARGRVAGIVINPAAWTHTSVALRDALAAVELPVVEV HLSNVHQREAFRHHSYVSPVALGVICGFGSLGYRLALEHFAERFEAAA

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0245 gene of Pseudomonas aeruginosa PAO1, Chromosome (277331 to 277777 bp)

Displaying the first five SNPs out of a total of 58 found in all isolate strains ( View all 58 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa PA7	Transversion	g.277340C>G		Silent GCG(Ala) -> GCC(Ala)	SNP-408490	View
Pseudomonas aeruginosa PA7	Indel	g.277348_277349dupC		1 BP Duplication (Frameshift)	SNP-408702	View
Pseudomonas aeruginosa PA7	Transversion	g.277349A>T	p.Arg143Gln	Missense CGT(Arg) -> CAG(Gln)	SNP-408491	View
Pseudomonas aeruginosa PA7	Transversion	g.277350C>G	p.Arg143Gln	Missense CGT(Arg) -> CAG(Gln)	SNP-408492	View
Pseudomonas aeruginosa PA7	Indel	g.277354delC		1 BP Deletion (Frameshift)	SNP-408703	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Characterization of a 3-dehydroquinase gene from Actinobacillus pleuropneumoniae with homology to the eukaryotic genes qa-2 and QUTE.
Lalonde G, O'Hanley PD, Stocker BA, Denich KT.
Mol Microbiol. 1994 Jan;11(2):273-80.
PMID: 8170389