Pseudomonas Genome Database - Annotation for PA0265

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PA0265

Key to diagram

View in GBrowse:

18 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name	gabD	Alternate Gene Names
Locus tag	PA0265 (View 7 updates) Submit an Update	GenBank Identifier (GI)	15595462 (Precomputed BLAST results at BLINK )
Genomic location	299522 - 300973 (+)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG000262 Orthology uncertain because more than one top BLASTp hit was identified.
Product Information
Product Name	succinate-semialdehyde dehydrogenase (Class 2 )
Alternate Product Names
Subcellular localization	Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA00266
Systomonas	PA0265
RefSeq	NP_248956.1
GI	15595462
KEGG	PA0265
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Central intermediary metabolism
Amino acid biosynthesis and metabolism
Carbon compound catabolism

GO Accession

GO Term

Evidence

Molecular Function

GO:0004777	succinate-semialdehyde dehydrogenase activity	IEA
GO:0009013	succinate-semialdehyde dehydrogenase [NAD(P)+] activity	IEA
GO:0016620	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor	IEA

Biological Process

GO:0006520	amino acid metabolic process	ISS
GO:0044248	cellular catabolic process	ISS
GO:0015978	carbon utilization by utilization of organic compounds	ISS
GO:0008152	metabolic process	ISS
GO:0009450	gamma-aminobutyric acid catabolic process	IEA

Cellular Component

COG Prediction

Accession	Description	Category
COG1012	PutA, NAD-dependent aldehyde dehydrogenases [Energy production and conversion].	Energy production and conversion

Pathways

Name	Database	Xref
Tyrosine metabolism	PseudoCAP
Glutamate metabolism	PseudoCAP
Butanoate metabolism	PseudoCAP
ornithine degradation	PseudoCyc	ORNDEG-PWY
4-aminobutyrate degradation	PseudoCyc	4AMINOBUTMETAB-PWY
putrescine degradation	PseudoCyc	PUTDEG-PWY
arginine degradation VII	PseudoCyc	ARGDEG-PWY
Alanine, aspartate and glutamate metabolism	KEGG	pae00250	Computationally Generated
Tyrosine metabolism	KEGG	pae00350	Computationally Generated
Butanoate metabolism	KEGG	pae00650	Computationally Generated
Metabolic pathways	KEGG	pae01100	Computationally Generated

Reactions

EC Number

1.2.1.16

Enzyme Assays

PDF file(s) provided by Sigma-Aldrich:
Succinic Semialdehyde Dehydrogenase (1.2.1.16)

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

90% similar to GabD gene product of E. coli

Most Similar Human Homolog

ENSP00000350191, Percent Identity: 57.88%, E-Value: 4e-153, Ensembl Release: 56.
Description: Succinate-semialdehyde dehydrogenase, mitochondrial Precursor

Structure Features

PROSITE: PDOC00068Aldehyde dehydrogenases active site

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF00171	Aldedh, Aldehyde dehydrogenase family.	19	476	1e-173	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

AATTTTCCGACTTTCGTTGATTTTATCGAACGGGGTATTGCCCTTTGTCAGTGGTGGCCCTAGGATTCGTTTGAGATTTCAAACGCCCCCAAAGCCACAA CAACAGGAGACCGGGGAATTGGCATCAGTTCAGCCGGCCGCGTGCCCCTGGAATGACCTCTTTCTGACCTGCCCGTCGCTCGTCCGGCCTTTCGCGTTGC CGGGCCGCCGTGGTGCCGGCGTGGCGTTTCGTCCGCAATCCCTTTCCCGCACGAGTCTTTCGTATCCTCCCCGACGCCTGTCGGCGGTCGCGTTCGTCCG CGTCCGCCCGTTGCGCACGGTGGCCGGCCGGTAACGGCGACGCCGCTGGCACAGGGCGCAGGAAATGGAACACCGGGGCGCTGGCCCTTTCGAATAACCA GCAGGCAACACTTTCCGGTTACAACCGAACCAGACAACCAGGCACGCGGGGTAGTCGAGGTAGACCCGCTGCTCACGGCATTCCTCGAAGGAGACAGGTC

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGCAACTCAAAGATGCCAAGCTGTTCCGTCAACAAGCCTACGTCGATGGCGCCTGGGTGGATGCCGACAACGGCCAGACCATCAAGGTGAACAACCCGG CCACCGGCGAGATCATCGGCAGCGTGCCGAAGATGGGCGCCGCCGAGACCCGCCGCGCCATCGAGGCTGCCGACAAGGCCCTGCCGGCCTGGCGTGCGCT GACCGCCAAGGAGCGCGCCAACAAGCTGCGCCGCTGGTTCGACCTGATGATCGAGAACCAGGACGACCTGGCCCGCCTGATGACCATCGAGCAGGGCAAG CCGCTGGCCGAGGCCAAGGGCGAGATCGCCTACGCCGCCTCCTTCCTCGAATGGTTCGGCGAAGAAGCCAAGCGCATCTACGGCGACACCATTCCCGGCC ACCAGCCGGACAAGCGCATCATCGTGATCAAGCAGCCGATCGGCGTGACCGCGGCCATCACGCCGTGGAACTTCCCCTCGGCGATGATCACCCGCAAGGC CGGCCCGGCCCTGGCCGCCGGCTGCACCATGGTGCTCAAGCCTGCTTCGCAGACCCCGTATTCCGCCCTGGCCCTGGCCGAACTGGCAGAGCGCGCCGGC ATTCCGAAGGGCGTGTTCAGCGTGGTCACCGGTAGCGCCGGCGAAGTCGGCGGCGAGCTGACCAGCAACCCGATCGTGCGCAAGCTGACCTTCACCGGTT CCACCGAAATCGGCCGCCAACTGATGGCCGAGTGCGCCCAGGACATCAAGAAGGTGTCCCTGGAGCTGGGCGGCAACGCGCCGTTCATCGTGTTCGACGA CGCCGACCTGGACGCCGCCGTCGAGGGCGCGCTGATTTCCAAGTACCGCAATAACGGCCAGACCTGCGTCTGCGCCAACCGCCTGTACGTGCAGGACGGC GTGTACGACGCCTTCGTCGACAAGCTGAAGGCCGCCGTGGCCAAGCTGAACATCGGCAACGGCCTGGAAGCCGGCGTGACCACCGGTCCGCTGATCGACG CCAAGGCCGTGGCCAAGGTCGAGGAACACATCGCCGACGCGGTCTCCAAGGGCGCCAAGGTGGTTTCCGGCGGCAAGCCGCACGCCCTCGGCGGCACCTT CTTCGAGCCGACCATCCTGGTCGACGTGCCGAAGAACGCCCTGGTGTCCAAGGACGAGACCTTCGGCCCGCTGGCGCCGGTGTTCCGCTTCAAGGACGAG GCCGAGGTCATCGCCATGTCCAACGACACCGAGTTCGGCCTGGCGTCCTACTTCTACGCCCGCGACCTGGCCCGCGTGTTCCGCGTCGCCGAGCAACTGG AGTACGGCATGGTCGGCATCAACACCGGCCTGATCTCCAACGAAGTGGCGCCGTTCGGCGGCATCAAGGCCTCGGGCCTGGGCCGCGAAGGTTCCAAGTA CGGGATCGAGGACTACCTCGAGATCAAGTACCTCTGCCTGGGCGGTATCTGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MQLKDAKLFRQQAYVDGAWVDADNGQTIKVNNPATGEIIGSVPKMGAAETRRAIEAADKALPAWRALTAKERANKLRRWFDLMIENQDDLARLMTIEQGK PLAEAKGEIAYAASFLEWFGEEAKRIYGDTIPGHQPDKRIIVIKQPIGVTAAITPWNFPSAMITRKAGPALAAGCTMVLKPASQTPYSALALAELAERAG IPKGVFSVVTGSAGEVGGELTSNPIVRKLTFTGSTEIGRQLMAECAQDIKKVSLELGGNAPFIVFDDADLDAAVEGALISKYRNNGQTCVCANRLYVQDG VYDAFVDKLKAAVAKLNIGNGLEAGVTTGPLIDAKAVAKVEEHIADAVSKGAKVVSGGKPHALGGTFFEPTILVDVPKNALVSKDETFGPLAPVFRFKDE AEVIAMSNDTEFGLASYFYARDLARVFRVAEQLEYGMVGINTGLISNEVAPFGGIKASGLGREGSKYGIEDYLEIKYLCLGGI

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0265 gene of Pseudomonas aeruginosa PAO1, Chromosome (299522 to 300973 bp)

Displaying the first five SNPs out of a total of 53 found in all isolate strains ( View all 53 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa PA7	Transversion	g.299560C>G		Silent GCC(Ala) -> GCG(Ala)	SNP-483115	View
Pseudomonas aeruginosa PA7	Transition	g.299564G>A	p.Val15Ile	Missense GTC(Val) -> ATC(Ile)	SNP-483116	View
Pseudomonas aeruginosa PA7	Transition	g.299592A>G	p.Asn24Ser	Missense AAC(Asn) -> AGC(Ser)	SNP-483117	View
Pseudomonas aeruginosa PA7	Transition	g.299636A>G	p.Ile39Val	Missense ATC(Ile) -> GTC(Val)	SNP-483118	View
Pseudomonas aeruginosa PA7	Transversion	g.299659C>G		Silent GGC(Gly) -> GGG(Gly)	SNP-483119	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Isolation and properties of Escherichia coli K-12 mutants impaired in the utilization of gamma-aminobutyrate.
Metzer E, Levitz R, Halpern YS.
J Bacteriol. 1979 Mar;137(3):1111-8.
PMID: 374339
Molecular organization of the Escherichia coli gab cluster: nucleotide sequence of the structural genes gabD and gabP and expression of the GABA permease gene.
Niegemann E, Schulz A, Bartsch K.
Arch Microbiol. 1993;160(6):454-60.
PMID: 8297211