Pseudomonas Genome Database - Annotation for PA0266

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PA0266

Key to diagram

View in GBrowse:

16 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name	gabT	Alternate Gene Names
Locus tag	PA0266 (View 7 updates) Submit an Update	GenBank Identifier (GI)	15595463 (Precomputed BLAST results at BLINK )
Genomic location	301218 - 302498 (+)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG000263
Product Information
Product Name	4-aminobutyrate aminotransferase (Class 2 )
Alternate Product Names	gabA transaminase
Subcellular localization	Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA00267
Systomonas	PA0266
RefSeq	NP_248957.1
GI	15595463
KEGG	PA0266
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Central intermediary metabolism
Amino acid biosynthesis and metabolism
Carbon compound catabolism

GO Accession

GO Term

Evidence

Molecular Function

GO:0003867

4-aminobutyrate transaminase activity

IEA

Biological Process

GO:0006520	amino acid metabolic process	ISS
GO:0044248	cellular catabolic process	ISS
GO:0015978	carbon utilization by utilization of organic compounds	ISS
GO:0008152	metabolic process	ISS
GO:0009450	gamma-aminobutyric acid catabolic process	IEA

Cellular Component

COG Prediction

Accession	Description	Category
COG0160	GabT, 4-aminobutyrate aminotransferase and related aminotransferases [Amino acid transport and metabolism].	Amino acid transport and metabolism

Pathways

Name	Database	Xref
Beta-Alanine metabolism	PseudoCAP
Propanoate metabolism	PseudoCAP
Glutamate metabolism	PseudoCAP
Alanine and Aspartate metabolism	PseudoCAP
Butanoate metabolism	PseudoCAP
ornithine degradation	PseudoCyc	ORNDEG-PWY
4-aminobutyrate degradation	PseudoCyc	4AMINOBUTMETAB-PWY
putrescine degradation	PseudoCyc	PUTDEG-PWY
arginine degradation II	PseudoCyc	ARGDEG-II-PWY
arginine degradation IV	PseudoCyc	ARGDEG-IV-PWY
arginine degradation VII	PseudoCyc	ARGDEG-PWY
Alanine, aspartate and glutamate metabolism	KEGG	pae00250	Computationally Generated
Valine, leucine and isoleucine degradation	KEGG	pae00280	Computationally Generated
beta-Alanine metabolism	KEGG	pae00410	Computationally Generated
Propanoate metabolism	KEGG	pae00640	Computationally Generated
Butanoate metabolism	KEGG	pae00650	Computationally Generated
Metabolic pathways	KEGG	pae01100	Computationally Generated

Reactions

EC Number

2.6.1.19

Enzyme Assays

PDF file(s) provided by Sigma-Aldrich:
GABASE (2.6.1.19)

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

87% similar to GabT gene product of E. coli

Most Similar Human Homolog

ENSP00000231420, Percent Identity: 34.19%, E-Value: 4e-36, Ensembl Release: 56.
Description: Alanine--glyoxylate aminotransferase 2, mitochondrial Precursor

Structure Features

PROSITE: PDOC00519Aminotransferases class-III pyridoxal-phosphate attachment site

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF00202	Aminotran_3, Aminotransferase class-III.	27	362	1e-115	23.0
PF00155	Aminotran_1_2, Aminotransferase class I and II.	61	418	8e-05	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

CGAGGCCGAGGTCATCGCCATGTCCAACGACACCGAGTTCGGCCTGGCGTCCTACTTCTACGCCCGCGACCTGGCCCGCGTGTTCCGCGTCGCCGAGCAA CTGGAGTACGGCATGGTCGGCATCAACACCGGCCTGATCTCCAACGAAGTGGCGCCGTTCGGCGGCATCAAGGCCTCGGGCCTGGGCCGCGAAGGTTCCA AGTACGGGATCGAGGACTACCTCGAGATCAAGTACCTCTGCCTGGGCGGTATCTGAGCCCGGCGAGGAACGCATCCGGAGGGGCCGGACGGCAGGCAAGA GCTGCGCCGTCCGGTGTCGACGTTGCCGGTGGTGGCACCGGACGAACCGGGAGACGCGGTCGCCGATCGCGAAGCGGCTCCGAGCCCTGGCGGGCCTGCA AGTCCCGAGGCAGTCGATCATCGCATGCTGCCTTGGGGAGCTTCCTTCCCGCCTTCATCCTTTGACCCGGCCGACCCGATGAGCGGCCACTGAGGAAGCC

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGAGCAAGACCAACGAATCCCTGCTGAAGCGTCGCCAGGCCGCGGTACCCCGCGGCGTGGGCCAGATCCACCCGGTCGTCGCCGAGCGCGCCGAGAACT CCACCGTCTGGGACGTGGAAGGCCGCGAGTACATCGACTTCGCCGGCGGCATCGCCGTGCTGAACACCGGCCACCTGCATCCGAAGGTGATCGCCGCGGT CCAAGAGCAACTGGGCAAGCTGTCCCACACCTGCTTCCAGGTGCTGGCCTACGAACCCTACATCGAGCTGGCCGAGGAAATCGCCAAGCGCGTGCCGGGC GACTTCCCGAAGAAGACCCTGCTGGTCACCTCCGGCTCCGAAGCCGTGGAGAACGCCGTCAAGATCGCCCGCGCCGCCACCGGCCGCGCTGGCGTGATCG CCTTCACCGGCGCGTACCACGGCCGCACCATGATGACCCTGGGCCTGACCGGCAAGGTGGTCCCGTACTCCGCCGGCATGGGCCTGATGCCGGGCGGCAT CTTCCGCGCCCTGGCGCCCTGCGAGCTGCACGGCGTGAGCGAGGACGACTCCATCGCCAGCATCGAGCGCATCTTCAAGAACGATGCCCAGCCCCAGGAC ATCGCCGCGATCATCATCGAGCCGGTGCAGGGCGAGGGTGGCTTCTACGTCAACTCCAAGTCCTTCATGCAGCGCCTGCGCGCCCTGTGCGACCAGCACG GCATCCTGCTGATCGCCGACGAAGTGCAGACCGGCGCCGGCCGTACCGGCACCTTCTTCGCTACCGAGCAACTGGGCATCGTTCCTGACCTGACCACCTT CGCCAAGTCGGTCGGCGGCGGCTTCCCGATCTCCGGCGTGGCCGGCAAGGCCGAGATCATGGACGCCATCGCGCCCGGCGGCCTGGGCGGCACCTATGCC GGCAGCCCGATCGCCTGCGCCGCGGCACTGGCCGTGCTGAAGGTGTTCGAGGAAGAGAAGCTGTTGGAACGTTCGCAAGCCGTCGGCGAGCGCCTCAAGG CCGGCCTGCGCGAGATCCAGGCCAAGCACAAGGTGATCGGCGACGTTCGTGGCCTGGGTTCGATGGTGGCCATCGAGCTGTTCGAGGGCGGCGATACCCA CAAGCCGGCCGCCGAGCTGGTCAGCAAGATCGTCGTCCGTGCGCGCGAGAAGGGCCTGATCCTGCTCTCCTGCGGCACCTACTACAACGTCATCCGCTTC CTCATGCCCGTCACCATCCCCGACGCGCAACTGGAGAAGGGCCTGGCCATCCTGGCCGAGTGCTTCGACGAACTCGCTTAA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MSKTNESLLKRRQAAVPRGVGQIHPVVAERAENSTVWDVEGREYIDFAGGIAVLNTGHLHPKVIAAVQEQLGKLSHTCFQVLAYEPYIELAEEIAKRVPG DFPKKTLLVTSGSEAVENAVKIARAATGRAGVIAFTGAYHGRTMMTLGLTGKVVPYSAGMGLMPGGIFRALAPCELHGVSEDDSIASIERIFKNDAQPQD IAAIIIEPVQGEGGFYVNSKSFMQRLRALCDQHGILLIADEVQTGAGRTGTFFATEQLGIVPDLTTFAKSVGGGFPISGVAGKAEIMDAIAPGGLGGTYA GSPIACAAALAVLKVFEEEKLLERSQAVGERLKAGLREIQAKHKVIGDVRGLGSMVAIELFEGGDTHKPAAELVSKIVVRAREKGLILLSCGTYYNVIRF LMPVTIPDAQLEKGLAILAECFDELA

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0266 gene of Pseudomonas aeruginosa PAO1, Chromosome (301218 to 302498 bp)

Displaying the first five SNPs out of a total of 39 found in all isolate strains ( View all 39 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa PA7	Transition	g.301346G>A		Silent GAG(Glu) -> GAA(Glu)	SNP-483155	View
Pseudomonas aeruginosa PA7	Transition	g.301376G>A		Silent GTG(Val) -> GTA(Val)	SNP-483156	View
Pseudomonas aeruginosa C3719	Transition	g.301421A>G		Silent CAA(Gln) -> CAG(Gln)	SNP-34318	View
Pseudomonas aeruginosa 2192	Transition	g.301421A>G		Silent CAA(Gln) -> CAG(Gln)	SNP-46711	View
Pseudomonas aeruginosa PA14	Transition	g.301421A>G		Silent CAA(Gln) -> CAG(Gln)	SNP-151824	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Stereospecific production of the herbicide phosphinothricin (glufosinate) by transamination: isolation and characterization of a phosphinothricin-specific transaminase from Escherichia coli.
Schulz A, Taggeselle P, Tripier D, Bartsch K.
Appl Environ Microbiol. 1990 Jan;56(1):1-6.
PMID: 2178550
Molecular analysis of two genes of the Escherichia coli gab cluster: nucleotide sequence of the glutamate:succinic semialdehyde transaminase gene (gabT) and characterization of the succinic semialdehyde dehydrogenase gene (gabD).
Bartsch K, von Johnn-Marteville A, Schulz A.
J Bacteriol. 1990 Dec;172(12):7035-42.
PMID: 2254272