Pseudomonas Genome Database - Annotation for PA0296

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PA0296

Key to diagram

View in GBrowse:

24 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name	spuI	Alternate Gene Names
Locus tag	PA0296 (View 7 updates) Submit an Update	GenBank Identifier (GI)	15595493 (Precomputed BLAST results at BLINK )
Genomic location	334455 - 333079 (-)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG000293 Possible inparalog involved. Orthology uncertain because more than one top BLASTp hit was identified.
Product Information
Product Name	probable glutamine synthetase (Class 3 )
Alternate Product Names
Subcellular localization	Cytoplasmic (Class 1) Cytoplasmic (Class 2) Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA00297
Systomonas	PA0296
RefSeq	NP_248987.1
GI	15595493
KEGG	PA0296
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Putative enzymes

Pathways

Name	Database	Xref
glutamine - glutamate pathway II	PseudoCyc	GLNSYN-PWY
ammonia assimilation cycle	PseudoCyc	AMMASSIM-PWY
Alanine, aspartate and glutamate metabolism	KEGG	pae00250	Computationally Generated
Arginine and proline metabolism	KEGG	pae00330	Computationally Generated
Nitrogen metabolism	KEGG	pae00910	Computationally Generated
Metabolic pathways	KEGG	pae01100	Computationally Generated
Two-component system	KEGG	pae02020	Computationally Generated

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

63% similar to putative glutamine synthetase YcjK [Escherichia coli]

Most Similar Human Homolog

ENSP00000359691, Percent Identity: 24.25%, E-Value: 8e-21, Ensembl Release: 56.
Description: Lengsin

Structure Features

BL00180: Glutamine synthetase signature 1

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF00120	Gln-synt_C, Glutamine synthetase, catalytic domain.	121	380	9e-78	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

ACGGGGCTCGACGTTCGACGGCGAGCCAGTGAAGAGCAAGCCGTCGACGCTGTCCAGCAGGGCTGCCTGGTCGATCGACTCGCCCAGCGCAGGAATGATC AGCGGGAGACCGCCAGCGCCGTTGACGACGGCACGCAGATATTTGTCGCCGGCAATATGGTAGGGATGAAGACCGATCTGCTTGGTGCAGGCGGTGACGC CGATTAACGGCAGGCGAGACATGAGACACCCGTTGTTTGTAGATGTTATGGGGTTTGGCCGAGCTTAGCGTTGTTCATTTTTTTACACAATAGGCCTGTA AAAAATTGAACACGCACTCCTGAACACCCTTCGACGCGAGGTTTTTTTCGGCTTTTCGTCCGGCGAAAATGCCCTGAAAACGGCCTCAACGCCCCACAAG AGGGCGTTTTTGGCGGCTATTGACATCCCACAGGCTTTCGGATTGACTCATAGCCTACCCCCCGCATGATTGAAATTTTTAACAAAAAAGGTGTTGCATC

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGTCGGTACCCCAGCGTGCCGTTCAGCTCACTGAACCGAGCGAGTTCCTCAAGGAACATCCCGAGGTCCAGTTCGTCGACCTCCTTATTGCAGACATGA ATGGCGTGGTTCGCGGCAAGCGGATCGAACGCAACAGTCTGAACAAAGTCTTCGAAAAAGGTATCAACCTTCCCGCTTCCCTGTTCGCCCTCGATATCAC CGGCTCCACCGTAGAGAGTACCGGCCTGGGTCTCGACATCGGCGACGCCGACCGGATCTGCTACCCGATCCCCGGCACCCTCTCCATGGAACCCTGGCAG AAGCGCCCGACCGCGCAACTGCTGATGACCATGCACGAACTGGAAGGCGAGCCGTTCTTCGCCGACCCCCGCGAAGTCCTGCGCCAGGTGGTGGCCAGGT TCACCGAGATGGAACTGACCATCGTCGCCGCCTTCGAGCTGGAGTTCTACCTGATCGACCAGGAGAACGTGAACGGCCGGCCGCAACCGCCGCGCTCGCC GATCTCCGGCAAACGTCCGCAGTCGGTGCAGGTGTACTCCATCGACGACCTCGACGAATACGTCGAGTGCCTCCAGGACATCATCGACGGCGCCCGCGCC CAGGGCATCCCGGCCGACGCCATCGTCGCCGAATCCGCACCGGCGCAGTTCGAGGTCAACCTCAACCACGTCAACGACGCCCTCAAGGCCTGCGACCACG CGGTGCTGCTCAAGCGCCTGGTCAAGAACATCGCCTACGACCACGAGATGGACACCACCTTCATGGCCAAGCCCTATCCGGGCCAGGCCGGGAACGGACT GCACGTGCATATCTCGCTGCTCGACAAGCATGGCAACAACATCTTCACCAGCGAGGATCCCGAGCAGAACGCCGCATTGCGCCATGCGATCGGCGGTGTG CTCGAGACCCTGCCGGCCTCCATGGCCTTCCTCTGCCCGAACGTCAACTCCTACCGCCGCTTCGGTTCGCAGTTCTACGTGCCGAACGCGCCGAGCTGGG GCCTGGACAACCGCACCGTGGCCCTGCGCGTGCCCACCGGCAGCCCGGACGCGGTACGCCTGGAACACCGCGTGGCCGGCGCCGACGCCAACCCCTACCT GCTACTGGCAGCGGTGCTGGCAGGCGTTCATCACGGGCTGACCAACAAGGTCGAGCCGGGCGCGCCGATCGAAGGCAACTCCTACGAGCAGATGGAGCCG AGCCTGCCGAACAACCTGCGCGACGCCCTGCGCGAGCTGGACGAAAGCGAGATCATGGCGAAGTACATCGACCCGAAGTACATCGACATCTTCGTCGCCT GCAAGGAAAGCGAGCTGGAGGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTCTGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MSVPQRAVQLTEPSEFLKEHPEVQFVDLLIADMNGVVRGKRIERNSLNKVFEKGINLPASLFALDITGSTVESTGLGLDIGDADRICYPIPGTLSMEPWQ KRPTAQLLMTMHELEGEPFFADPREVLRQVVARFTEMELTIVAAFELEFYLIDQENVNGRPQPPRSPISGKRPQSVQVYSIDDLDEYVECLQDIIDGARA QGIPADAIVAESAPAQFEVNLNHVNDALKACDHAVLLKRLVKNIAYDHEMDTTFMAKPYPGQAGNGLHVHISLLDKHGNNIFTSEDPEQNAALRHAIGGV LETLPASMAFLCPNVNSYRRFGSQFYVPNAPSWGLDNRTVALRVPTGSPDAVRLEHRVAGADANPYLLLAAVLAGVHHGLTNKVEPGAPIEGNSYEQMEP SLPNNLRDALRELDESEIMAKYIDPKYIDIFVACKESELEEFEHSISDLEYNWYLHTV

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

PubMed

Functional analysis and regulation of the divergent spuABCDEFGH-spuI operons for polyamine uptake and utilization in Pseudomonas aeruginosa PAO1.
Lu CD, Itoh Y, Nakada Y, Jiang Y.
J Bacteriol. 2002 Jul;184(14):3765-73.
PMID: 12081945
PSORTb 3.0: Improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes.
Yu NY, Wagner JR, Laird MR, Melli G, Rey S, Lo R, Dao P, Sahinalp SC, Ester M, Foster LJ, Brinkman FS.
Bioinformatics. 2010 May 13; [Epub ahead of print]
PMID: 20472543