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               PA0298               
Key to diagram


View in GBrowse: 24 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name spuB  Alternate Gene Names
Locus tag PA0298 (View 6 updates) Submit an Update GenBank Identifier (GI) 15595495 (Precomputed BLAST results at BLINK )
Genomic location 335527 - 336885 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
POG000295 
Orthology uncertain because more than one top BLASTp hit was identified.
Product Information
Product Name probable glutamine synthetase (Class 1  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Cytoplasmic (Class 2Explanation of Localization Confidence)
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA00299
Systomonas PA0298
RefSeq NP_248989.1
GI 15595495
KEGG PA0298
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
spuA probable glutamine amidotransferase
spuB probable glutamine synthetase
spuC putrescine aminotransferase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13162
Genomic Context
Genomic context Explanation of Genomic Context Field - One of eight genes in the spuABCDEFGH operon
- Functions in spermidine and putrescine utilization, and probable regulation of the spu operon by the CbrAB two-component system.
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Putative enzymes
GO Accession GO Term Evidence
Molecular Function
GO:0003824 catalytic activity IDA
Biological Process
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0174 GlnA, Glutamine synthetase [Amino acid transport and metabolism]. Amino acid transport and metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
glutamine - glutamate pathway II PseudoCyc GLNSYN-PWY
ammonia assimilation cycle PseudoCyc AMMASSIM-PWY
Alanine, aspartate and glutamate metabolism KEGG pae00250 Computationally Generated
Arginine and proline metabolism KEGG pae00330 Computationally Generated
Nitrogen metabolism KEGG pae00910 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Two-component system KEGG pae02020 Computationally Generated
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 50% similar to putative glutamine synthetase YcjK [Escherichia coli]
Most Similar Human Homolog
ENSP00000359691, Percent Identity: 26.16%, E-Value: 6e-27, Ensembl Release: 56.
Description: Lengsin
Structure Features
Structure Features Explanation of Structure Features Field BL00180: Glutamine synthetase signature 1
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00120 Gln-synt_C, Glutamine synthetase, catalytic domain. 114 374 4e-88 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region GGTCCGCGCCGCCATCGACGCCGGCATTCCGGTCCTCGGCATCTGCCGCGGTTTCCAGGAAATGAACGTGGCTTTCGGCGGCAGCCTGCACCAGAAGGTG CACGAGGTCGGAACCTTCATGGACCACCGCGAGCCGGCCGACCAGCCGCTCGAGGTTCAGTACGCGCCGCGCCATGCCATGCATGTGCAGCCGGGCGGAG TGCTCGCCGGTATCGGCTTGCCGAGCGAGTTCCAGGTCAATTCCATCCATGGCCAGGGCGTTGATCGTCTGGCGCCGGGCCTTCGAGTAGAAGCACTGGC GCCTGACGGGTTGGTCGAAGCCATCTCCGTAGAAGGTGCAAAAGCCTTCGCTCTTGGTGTGCAATGGCACCCGGAGTGGCAGGTTCTGACGAACCCCAAC TATCTCGCTATCTTCCAGGCTTTCGGTAAGGCCTGCAGCAAGAGGGCGGGGCAACGTTGAGTCCGGCTGTTGGGGCCGGACTCTCAACCCTGAGGTCTTT

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGACTACCAAGTTAGACCAGCTGACCAGCTGGCTGAAGGAACGCAAGATCACCGAAGTGGAATGCCTGATTTCCGATCTGACAGGCATCGCCCGCGGCA AGATCTCGCCGACCAACAAATTCATCGCGGAAAAGGGCATGCGCCTGCCGGAGAGCGTTCTGCTGCAGACCGTCACCGGCGACTACGTGGAAGACGACAT TTACTACGACCTGCTCGACCCGGCCGACATCGATATGGTCTGCCGCCCGGACGAGAATGCCGTGTTCCTCGTCCCCTGGGCCATCGAGCCCACCGCGATG GTGATCCACGACACCTTCGACAAGCTCGGCAATCCCATCGAGCTGTCGCCGCGCAACATCCTCAAGCGCGTGTTGAAGATGTATGCCGACAAGGGCTGGC GGCCGATCGTGGCGCCAGAGATGGAGTTCTACCTGACCAAGCGCAGCGACGACCCCGACTATCCGTTGCAGGCCCCGGTGGGCCGCTCCGGACGCCAGGA AACCGGGCGTCAGTCGTTCTCCATCGACGCCGCCAACGAGTTCGACCCGCTGTTCGAGGACATGTACGACTGGTGCGAGGCCCAGGGCCTTGACCTGGAT ACGCTGATCCACGAGGAAGGCACCGCGCAGATGGAGATCAACTTCCGTCATGGCGACGCCCTCGACCTGGCCGACCAGATCCTGGTGTTCAAGCGCACCA TGCGCGAGGCCGCGCTCAAGCACAACGTCGCCGCGACCTTCATGGCCAAGCCGATGACCGGCGAGCCGGGCAGCGCGATGCACCTGCACCAGAGCATCGT CGACGTGAAGACCGGCAAGAACATCTTCTCCAATGCCGACGGCACCATGAGCGAGCTGTTCCTGCACCATATCGGCGGCCTGCAGAAGTTCATCCCGGAA GTGCTGCCGCTGTTCGCGCCCAACGTGAACTCGTTCCGCCGCTTCCTGCCCGATACCTCGGCGCCGGTGAACGTCGAGTGGGGCGAAGAGAACCGTACCG TCGGCCTGCGCGTGCCGGACTCCAGCCCGGAGAACCGCCGGGTCGAGAACCGTCTCGCCGGCGCCGACGCCAACCCTTACCTGGCGCTGGCTGCCAGCCT GCTGTGCGGCTACATCGGCATGGTCGAAGGCATCAAGCCGAGCGCCCAGGTCAAAGGTCGCGGCTATGAACGCCGCAACCTGCGCCTGCCGCTGACGATC GAAGCGGCGCTGGAGCGCATGGAGAACTGCAAGCCCCTCGAGCAGTATCTCGGCAGCAAGTTCATCAGCGGCTACGTCGCGGTGAAGCGTGCGGAGCACG AGAATTTCAAGCGGGTAATCAGCTCCTGGGAGCGGGAATTCCTCCTGCTTTCCGTTTGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MTTKLDQLTSWLKERKITEVECLISDLTGIARGKISPTNKFIAEKGMRLPESVLLQTVTGDYVEDDIYYDLLDPADIDMVCRPDENAVFLVPWAIEPTAM VIHDTFDKLGNPIELSPRNILKRVLKMYADKGWRPIVAPEMEFYLTKRSDDPDYPLQAPVGRSGRQETGRQSFSIDAANEFDPLFEDMYDWCEAQGLDLD TLIHEEGTAQMEINFRHGDALDLADQILVFKRTMREAALKHNVAATFMAKPMTGEPGSAMHLHQSIVDVKTGKNIFSNADGTMSELFLHHIGGLQKFIPE VLPLFAPNVNSFRRFLPDTSAPVNVEWGEENRTVGLRVPDSSPENRRVENRLAGADANPYLALAASLLCGYIGMVEGIKPSAQVKGRGYERRNLRLPLTI EAALERMENCKPLEQYLGSKFISGYVAVKRAEHENFKRVISSWEREFLLLSV

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0298 gene of Pseudomonas aeruginosa PAO1, Chromosome (335527 to 336885 bp)


Displaying the first five SNPs out of a total of 29 found in all isolate strains ( View all 29 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA7 Transition g.335604T>C
Silent
GAT(Asp) -> GAC(Asp) 		SNP-495310 View
Pseudomonas aeruginosa PA7 Transition g.335646A>G
Silent
AAA(Lys) -> AAG(Lys) 		SNP-495311 View
Pseudomonas aeruginosa PA7 Transition g.335838C>T
Silent
GAC(Asp) -> GAT(Asp) 		SNP-495312 View
Pseudomonas aeruginosa PA7 Transition g.335859T>C
Silent
AAT(Asn) -> AAC(Asn) 		SNP-495313 View
Pseudomonas aeruginosa PA7 Transition g.335868G>A
Silent
GAG(Glu) -> GAA(Glu) 		SNP-495314 View
Select to view SNPs in a specific strain
SNP Map
PubMed Functional analysis and regulation of the divergent spuABCDEFGH-spuI operons for polyamine uptake and utilization in Pseudomonas aeruginosa PAO1.
Lu CD, Itoh Y, Nakada Y, Jiang Y.
J Bacteriol. 2002 Jul;184(14):3765-73.
PMID: 12081945
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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