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               PA0399               
Key to diagram


View in GBrowse: 5 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name   Alternate Gene Names
Locus tag PA0399 (View 7 updates) Submit an Update GenBank Identifier (GI) 15595596 (Precomputed BLAST results at BLINK )
Genomic location 440638 - 442011 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name cystathionine beta-synthase (Class 2  Explanation of Product Name Confidence)
Alternate Product Names methylcysteine synthase, beta-thionase, serine sulfhydrase
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA00400
Systomonas PA0399
RefSeq NP_249090.1
GI 15595596
KEGG PA0399
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
PA0399 cystathionine beta-synthase
PA0400 probable cystathionine gamma-lyase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13185
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Amino acid biosynthesis and metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0004122 cystathionine beta-synthase activity IEA
Biological Process
GO:0006520 amino acid metabolic process ISS
GO:0019344 cysteine biosynthetic process IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0031 CysK, Cysteine synthase [Amino acid transport and metabolism]. Amino acid transport and metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Cysteine metabolism PseudoCAP
Glycine, serine and threonine metabolism PseudoCAP
homocysteine degradation I PseudoCyc HOMOCYSDEGR-PWY
Glycine, serine and threonine metabolism KEGG pae00260 Computationally Generated
Cysteine and methionine metabolism KEGG pae00270 Computationally Generated
Selenoamino acid metabolism KEGG pae00450 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 4.2.1.22
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 53% similar to cystathionine beta-synthase [Homo sapiens]
53% similar to cysM2 putative cystathionine beta-synthase [Mycobacterium tuberculosis]
Most Similar Human Homolog
ENSP00000352643, Percent Identity: 34.62%, E-Value: 3e-62, Ensembl Release: 56.
Description: Cystathionine beta-synthase
Structure Features
Structure Features Explanation of Structure Features Field PF00291/PS00901: Cysteine synthase/cystathionine beta-synthase P-phosphate attachment site
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00291 PALP, Pyridoxal-phosphate dependent enzyme. 9 303 8e-63 23.0
PF00571 CBS, CBS domain pair. 344 453 9e-14 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region ATCATCGACCTGTTCCTCATCCCGTCGATGGACCGCGAGGCCGACCGGCGCTTCCAGGGCGGCAACCTGGACTACAACGTGGCCTGGATCCTGCTGACCT TCCTCGGCGTGCTCGGCGTGCACAGGATGTACATGGGCAAGTGGATCACCGGGATCATCTACCTGTTCACCGGCGGCCTGTTCTTCCTCGGCGTGCTCTA CGACTTCTGGACCCTCAACGGACAGGTGTCCGAGCGCAACGGGCCGCAGCGCATCTGAGGCTCGTCGCCAGGTGCGAGAAGCCCGCCATCCGGCGGGCTT TTTCATGGGTGGCGCCACGGTCGGCAGGGAATTCCTCCGGCGGGACTTAAGGAACCAAAGGTTCTAACGTTCTACCTGTAGGTTATTGGCGCCCGCGCGC GCCAGCCCCTATGGTCTGCAAGCCCCAGAACGGATAGGTGCAATGGCTCACGAGGCCGCGCACCGTATCGCTCCGTGCGGAAACACCGAAAGGAGAAAAG

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGACTTCCGACTCACGTCCCGCCGTGCTCGACCTGATCGGCAACACCCCGCTGGTTCGCGTGACGCGCTTCGATACCGGCCCCTGCACCCTGTACCTCA AGCTCGAATCGCAGAACCCCGGCGGCTCCATCAAGGATCGCATCGGTGTCGCCATGATCGAGGCCGCCGAGCGTGACGGCCGCCTGCGGCCCGGTGGCAC CATCGTCGAGGCCACCGCCGGCAACACCGGTCTCGGCCTGGCCCTGGTCGGCCGCGCGAAGGGCTACCGGGTGGTGCTGGTGGTGCCGGACAAGATGTCC ACCGAGAAGGTCCTGCACCTGCGCGCCATGGGCGCCGAGGTGCACATCACCCGATCGGACGTCGGCAAGGGCCATCCGGAGTACTACCAGGACGTTGCCG CGCGCCTGGCGCAGGATATTCCCGGCGCCTTCTTCGCCGACCAGTTCAACAACCCGGCCAACCCGCTGGCCCACGAGTGCGGCACCGGCCCGGAGCTCTG GGCGCAGACCGGCCACGATCTCGACGCCATCGTCGTCGGCGTCGGCTCCTCCGGTACCCTGACCGGCCTTACCCGGTTCTTCCAGAAGGTCCAGCCGGAG CTGGAGATGGTGCTCGCCGATCCCGAGGGCTCGATCATGGCCGAGTACTCCCGCTCCGGCACCCTGGGTACGCCGGGTTCCTGGGCGGTGGAGGGCATCG GCGAGGACTTCGTACCGGCCATCGCCGACCTCTCCAGCGTGCGCCATGCCTATTCCATCAGCGACGAGGAAAGCTTCGCCATGGCCCGCGAACTGTTGCG CGTCGAGGGCATTCCCGGCGGCTCCTCCACCGGCACCCTGCTGGCCGCGGCGCTGCGCTTCTGTCGCGAGCAGAAGGAGCCGAAGCGGGTGGTCAGCTTC GTCTGCGACACCGGCACCCGCTACCTGTCGAAGATCTACAACGACCAGTGGATGACCGACCAGGGCCTGCTGCAACGCAAGCACTACGGCGACCTGCGCG ACATCATCGCGCGGCGCTTCGAGGAGGGCCGGGTGATCAGCGTCGGTCCGGACGACACCCTGCTCACCGCCTTCCAGCGCATGCGCCTGGCCGACGTTTC GCAATTGCCGGTGCTGGACGATGGAAAACTGGTCGGTGTGATCGACGAGTCGGATATCCTGCTCGGTGTCCACGCCGACGCGCCACGCTTCCGCGATGCG GTAAGCAGCGCGATGAACGCCGCGCCGGAGACCCTCGCGCCCGGCGCCAGCCTGGCCCAGTTGCAGGCGGTCCTGGACCGCGGCCTGGTGGCGATCGTTG CCGATGCCAGCGGCTTCCACGGCCTGATCACCCGTTTCGACCTGTTGAACCATTTGCGGAGAAAGCTCGCATGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MTSDSRPAVLDLIGNTPLVRVTRFDTGPCTLYLKLESQNPGGSIKDRIGVAMIEAAERDGRLRPGGTIVEATAGNTGLGLALVGRAKGYRVVLVVPDKMS TEKVLHLRAMGAEVHITRSDVGKGHPEYYQDVAARLAQDIPGAFFADQFNNPANPLAHECGTGPELWAQTGHDLDAIVVGVGSSGTLTGLTRFFQKVQPE LEMVLADPEGSIMAEYSRSGTLGTPGSWAVEGIGEDFVPAIADLSSVRHAYSISDEESFAMARELLRVEGIPGGSSTGTLLAAALRFCREQKEPKRVVSF VCDTGTRYLSKIYNDQWMTDQGLLQRKHYGDLRDIIARRFEEGRVISVGPDDTLLTAFQRMRLADVSQLPVLDDGKLVGVIDESDILLGVHADAPRFRDA VSSAMNAAPETLAPGASLAQLQAVLDRGLVAIVADASGFHGLITRFDLLNHLRRKLA

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0399 gene of Pseudomonas aeruginosa PAO1, Chromosome (440638 to 442011 bp)


Displaying the first five SNPs out of a total of 115 found in all isolate strains ( View all 115 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa C3719 Transition g.440655T>C
Silent
CGT(Arg) -> CGC(Arg) 		SNP-18337 View
Pseudomonas aeruginosa PA14 Transition g.440655T>C
Silent
CGT(Arg) -> CGC(Arg) 		SNP-184529 View
Pseudomonas aeruginosa PA7 Transition g.440655T>C
Silent
CGT(Arg) -> CGC(Arg) 		SNP-484294 View
Pseudomonas aeruginosa PA7 Transition g.440682C>T
Silent
AAC(Asn) -> AAT(Asn) 		SNP-484295 View
Pseudomonas aeruginosa C3719 Transversion g.440732A>T p.Tyr32Phe
 Missense
TAC(Tyr) -> TTC(Phe) 		SNP-18338 View
Select to view SNPs in a specific strain
SNP Map
PubMed Human cystathionine beta-synthase cDNA: sequence, alternative splicing and expression in cultured cells.
Kraus JP, Le K, Swaroop M, Ohura T, Tahara T, Rosenberg LE, Roper MD, Kozich V.
Hum Mol Genet. 1993 Oct;2(10):1633-8.
PMID: 7903580
An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae.
Philipp WJ, Poulet S, Eiglmeier K, Pascopella L, Balasubramanian V, Heym B, Bergh S, Bloom BR, Jacobs WR Jr, Cole ST.
Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):3132-7.
PMID: 8610181
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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