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               PA0400               
Key to diagram


View in GBrowse: 11 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name   Alternate Gene Names metC, metB
Locus tag PA0400 (View 5 updates) Submit an Update GenBank Identifier (GI) 15595597 (Precomputed BLAST results at BLINK )
Genomic location 442008 - 443192 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name probable cystathionine gamma-lyase (Class 3  Explanation of Product Name Confidence)
Alternate Product Names cystathionase, gamma-cystathionase, cysteine desulfhydrase, cystine desulfhydrase, homoserine dehydratase, homoserine deaminase
Subcellular localization
Cytoplasmic (Class 2Explanation of Localization Confidence)
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA00401
Systomonas PA0400
RefSeq NP_249091.1
GI 15595597
KEGG PA0400
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
PA0399 cystathionine beta-synthase
PA0400 probable cystathionine gamma-lyase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13185
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Amino acid biosynthesis and metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0018826 methionine gamma-lyase activity IEA
Biological Process
GO:0009087 methionine catabolic process IEA
GO:0009093 cysteine catabolic process IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0626 MetC, Cystathionine beta-lyases/cystathionine gamma-synthases [Amino acid transport and metabolism]. Amino acid transport and metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Sulfur metabolism PseudoCAP
Selenoamino acid metabolism PseudoCAP
Nitrogen metabolism PseudoCAP
Methionine metabolism PseudoCAP
Glycine, serine and threonine metabolism PseudoCAP
Cysteine metabolism PseudoCAP
homocysteine degradation I PseudoCyc HOMOCYSDEGR-PWY
Glycine, serine and threonine metabolism KEGG pae00260 Computationally Generated
Cysteine and methionine metabolism KEGG pae00270 Computationally Generated
Selenoamino acid metabolism KEGG pae00450 Computationally Generated
Nitrogen metabolism KEGG pae00910 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 4.4.1.1
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 81% similar to cystathionine gamma-lyase-like protein [Stenotrophomonas maltophilia]
Most Similar Human Homolog
ENSP00000359976, Percent Identity: 51.77%, E-Value: 1e-98, Ensembl Release: 56.
Description: Cystathionine gamma-lyase
Structure Features
Structure Features Explanation of Structure Features Field PF01053: Gamma-family Cys/Met metabolism PLP-dependent enzyme
PS00868: Cys/Met metabolism enzymes pyridoxal-phosphate attachment site
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF01053 Cys_Met_Meta_PP, Cys/Met metabolism PLP-dependent enzyme. 16 392 1e-170 23.0
PF00266 Aminotran_5, Aminotransferase class-V. 76 210 2e-05 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region CAGAAGGAGCCGAAGCGGGTGGTCAGCTTCGTCTGCGACACCGGCACCCGCTACCTGTCGAAGATCTACAACGACCAGTGGATGACCGACCAGGGCCTGC TGCAACGCAAGCACTACGGCGACCTGCGCGACATCATCGCGCGGCGCTTCGAGGAGGGCCGGGTGATCAGCGTCGGTCCGGACGACACCCTGCTCACCGC CTTCCAGCGCATGCGCCTGGCCGACGTTTCGCAATTGCCGGTGCTGGACGATGGAAAACTGGTCGGTGTGATCGACGAGTCGGATATCCTGCTCGGTGTC CACGCCGACGCGCCACGCTTCCGCGATGCGGTAAGCAGCGCGATGAACGCCGCGCCGGAGACCCTCGCGCCCGGCGCCAGCCTGGCCCAGTTGCAGGCGG TCCTGGACCGCGGCCTGGTGGCGATCGTTGCCGATGCCAGCGGCTTCCACGGCCTGATCACCCGTTTCGACCTGTTGAACCATTTGCGGAGAAAGCTCGC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGAGCCAGCACGACCAGCATCCCGACGCCCCGGCCCAGGCCTTTGCGACCCGGGTCATCCACGCCGGGCAGGCCCCCGATCCCTCGACCGGCGCGATCA TGCCGCCGATCTACGCCAACTCCACCTATATCCAGGAAAGCCCCGGGGTACACAAGGGGCTCGACTACGGCCGCTCCCACAACCCGACCCGCTGGGCCCT GGAGCGCTGCGTGGCCGACCTCGAGGGCGGCACCCAGGCCTTCGCCTTCGCCTCCGGCCTGGCGGCGATCTCCTCGGTGCTGGAGCTGCTCGACGCCGGT TCCCACATCGTTTCCGGCAATGACCTCTACGGCGGTACCTTCCGCCTGTTCGAGCGGGTCCGCCGGCGCAGCGCCGGGCATCGCTTCAGCTTCGTCGACC CGACCGACCTGCAGGCCTTCGAGGCCGCGCTGACCCCGGAAACCCGCATGGTCTGGGTGGAAACCCCGAGCAACCCGCTGCTGCGCCTCACCGACCTGCG CGCCATCGCCCAGCTCTGCCGCGCCCGCGGCATCATCAGCGTGGCCGACAACACCTTCGCCAGCCCCTACATCCAGCGCCCCCTGGAGCTGGGCTTCGAC GTGGTGGTGCACTCCACCACCAAGTACCTGAACGGCCATTCCGACGTGATCGGCGGCATCGCCATCGTCGGCGACAACCCGGACCTGCGCGAGCGCCTGG GCTTCCTGCAGAACTCGGTGGGCGCCATCTCCGGGCCGTTCGACGCCTTCCTCACCCTGCGCGGGGTGAAGACCCTGGCGCTGCGCATGGAGCGCCATTG CAGTAACGCCCTGGCCCTGGCCCAGTGGCTGGAGCGCCAGCCGCAAGTGGCGCGGGTCTACTACCCCGGCCTGGCTTCGCACCCGCAGCATGAGCTGGCG AAACGGCAGATGCGTGGCTTCGGCGGGATGATCTCCCTCGACCTGCGTTGCGACCTGGCCGGCGCCCGGCGCTTCCTGGAGAACGTGCGGATCTTCTCCC TGGCGGAGAGCCTGGGCGGGGTGGAAAGCCTGATCGAGCATCCGGCGATCATGACCCACGCCAGCATCCCGGCGGAAACCCGCGCCGACCTGGGCATTGG TGACTCGCTGATCCGCCTGTCGGTGGGCGTCGAGGCGCTGGAGGACCTGCAGGCCGACCTGGCGCAGGCGCTGGCGAAGATCTGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MSQHDQHPDAPAQAFATRVIHAGQAPDPSTGAIMPPIYANSTYIQESPGVHKGLDYGRSHNPTRWALERCVADLEGGTQAFAFASGLAAISSVLELLDAG SHIVSGNDLYGGTFRLFERVRRRSAGHRFSFVDPTDLQAFEAALTPETRMVWVETPSNPLLRLTDLRAIAQLCRARGIISVADNTFASPYIQRPLELGFD VVVHSTTKYLNGHSDVIGGIAIVGDNPDLRERLGFLQNSVGAISGPFDAFLTLRGVKTLALRMERHCSNALALAQWLERQPQVARVYYPGLASHPQHELA KRQMRGFGGMISLDLRCDLAGARRFLENVRIFSLAESLGGVESLIEHPAIMTHASIPAETRADLGIGDSLIRLSVGVEALEDLQADLAQALAKI

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0400 gene of Pseudomonas aeruginosa PAO1, Chromosome (442008 to 443192 bp)


Displaying the first five SNPs out of a total of 98 found in all isolate strains ( View all 98 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA7 Transversion g.442026C>A p.His7Asn
 Missense
CAT(His) -> AAC(Asn) 		SNP-484366 View
Pseudomonas aeruginosa PA7 Transition g.442028T>C p.His7Asn
 Missense
CAT(His) -> AAC(Asn) 		SNP-484367 View
Pseudomonas aeruginosa C3719 Transition g.442052T>C
Silent
TTT(Phe) -> TTC(Phe) 		SNP-18348 View
Pseudomonas aeruginosa PACS2 Transition g.442052T>C
Silent
TTT(Phe) -> TTC(Phe) 		SNP-104633 View
Pseudomonas aeruginosa PA14 Transition g.442052T>C
Silent
TTT(Phe) -> TTC(Phe) 		SNP-184543 View
Select to view SNPs in a specific strain
SNP Map
PubMed  


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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