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               PA0402               
Key to diagram


View in GBrowse: 16 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name pyrB  Alternate Gene Names
Locus tag PA0402 (View 6 updates) Submit an Update GenBank Identifier (GI) 15595599 (Precomputed BLAST results at BLINK )
Genomic location 445691 - 444687 (-) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name aspartate carbamoyltransferase (Class 2  Explanation of Product Name Confidence)
Alternate Product Names aspartate transcarbamoylase
Subcellular localization
Cytoplasmic (Class 2Explanation of Localization Confidence)
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA00403
Systomonas PA0402
RefSeq NP_249093.1
GI 15595599
KEGG PA0402
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
PA0401 noncatalytic dihydroorotase-like protein
pyrB aspartate carbamoyltransferase
pyrR transcriptional regulator PyrR
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13186
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Amino acid biosynthesis and metabolism
Nucleotide biosynthesis and metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0004355 glutamate synthase (NADPH) activity ISS
GO:0004070 aspartate carbamoyltransferase activity IEA
Biological Process
GO:0006520 amino acid metabolic process ISS
GO:0009117 nucleotide metabolic process ISS
GO:0006522 alanine metabolic process ISS
GO:0006531 aspartate metabolic process ISS
GO:0006220 pyrimidine nucleotide metabolic process ISS
GO:0009220 pyrimidine ribonucleotide biosynthetic process IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0540 PyrB, Aspartate carbamoyltransferase, catalytic chain [Nucleotide transport and metabolism]. Nucleotide transport and metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Pyrimidine metabolism PseudoCAP
Alanine and Aspartate metabolism PseudoCAP
de novo biosynthesis of pyrimidine ribonucleotides PseudoCyc PWY0-162
his+purine+pyrimidine biosynthesis PseudoCyc PRPP-PWY
Pyrimidine metabolism KEGG pae00240 Computationally Generated
Alanine, aspartate and glutamate metabolism KEGG pae00250 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 2.1.3.2
Enzyme Assays
PDF file(s) provided by Sigma-Aldrich:
 Aspartate Transcarbamylase (2.1.3.2)
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 94% similar to P. putida aspartate carbamoyltransferase.
Most Similar Human Homolog
ENSP00000384510, Percent Identity: 32.14%, E-Value: 3e-34, Ensembl Release: 56.
Description: CAD protein
Structure Features
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF02729 OTCace_N, Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain. 18 164 2e-31 23.0
PF00185 OTCace, Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain. 168 318 3e-25 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region ATGGCCAGCGACCTGCGCGCCCATCTCGCCGAGCGCGGCATCGAGCGGCCGCGCTTCGTCGGCATCCATACCGGCGGCATCTGGGTCGCCGAGGCGCTGC TGAGGGAACTGGGCAACCAAGAGCCGCTGGGCACCCTCGACGTCTCCTTCTACCGCGACGACTTCACCCAGAACGGCCTGCATCCGCAGGTCCGCCCGTC GGCGCTGCCGTTCGAGATCGACGGCCAGCACCTGGTGCTGGTGGACGACGTGCTGATGAGCGGCCGTACCATCCGCGCCGCACTCAACGAACTGTTCGAC TACGGCCGTCCGGCCAGCGTGACCCTGGTCTGCCTGCTCGACCTGAACGCCCGGGAGTTGCCTATCCGCCCCGACGTGGTCGGCCAGACCCTGTCCCTGG GTCGCGATGAACGGGTAAAATTGGTCGGTCCCGCACCGCTCGCCCTCGAGCGCAAGGTCCTTTCCTCCGCTTCCTGATCCTCTTCACTAGGGCCTGCCCC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGCCGACAGACGCCAAGCGCCCGCTGCAGCTCAACGACCAGGGCCAGCTGCGCCACTTCATCTCGCTCGACGGATTGCCCCGCGAGCTGCTCACCGAAA TCCTCGATACCGCCGATTCCTTCCTGGAGGTCGGCGCCCGCGCGGTGAAAAAGGTCCCGCTGCTGCGCGGCAAGACCGTCTGCAACGTGTTCTTCGAGAA CTCCACGCGCACCCGCACCACCTTCGAGCTGGCCGCCCAGCGGCTGTCGGCCGACGTGATCAGCCTGAACGTATCCACCTCTTCCACCAGCAAGGGCGAG ACGCTCACCGACACCCTGCGCAACCTGGAGGCGATGGCCGCCGACATGTTCGTGGTGCGCCACAGCGACTCGGGCGCGGCGCACTTCATCGCCGAGCACG TCAGCCCCAACGTGGCGGTGATCAACGGCGGCGACGGACGCCACGCGCACCCCACCCAGGGGATGCTCGACATGCTGACCATCCGCCGGCACAAAGGGAA CTTCGAGCAGCTATCGGTGGCGATCGTCGGCGATATCCTGCATTCGCGAGTAGCCCGTTCGAACATGCTGGCGCTCAAGACCCTGGGTTGCCCGGACATC CGCGTGATCGCCCCGCGCACCCTCTTGCCGATCGGCCTGGAAGAGCAGTACGGCGTGCGCGTGTTCACCAACGCCGACGAAGGCCTGAAGGACGTCGACG TGGTGATCATGCTGCGCCTGCAACGCGAGCGCATGCAGGGTGGCCTGCTACCCAGCGAGGGCGAGTTCTTCAAGCTCTACGGCCTGACCGAGAAGCGCCT GAAGCTGGCCAAGCCGGATGCCATCGTCATGCACCCTGGCCCGATCAACCGTGGCGTGGAGATCGAGTCGGCGGTGGCCGACGGGGCCCAGTCGGTGATC CTCAACCAGGTCACCTACGGCATCGCCATCCGCATGGCGGTGCTGTCCATGGCCATGAGCGGCCAGAACACCCAACGCCAGCTGGAACAGGAGGACGCCG AATGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MPTDAKRPLQLNDQGQLRHFISLDGLPRELLTEILDTADSFLEVGARAVKKVPLLRGKTVCNVFFENSTRTRTTFELAAQRLSADVISLNVSTSSTSKGE TLTDTLRNLEAMAADMFVVRHSDSGAAHFIAEHVSPNVAVINGGDGRHAHPTQGMLDMLTIRRHKGNFEQLSVAIVGDILHSRVARSNMLALKTLGCPDI RVIAPRTLLPIGLEEQYGVRVFTNADEGLKDVDVVIMLRLQRERMQGGLLPSEGEFFKLYGLTEKRLKLAKPDAIVMHPGPINRGVEIESAVADGAQSVI LNQVTYGIAIRMAVLSMAMSGQNTQRQLEQEDAE

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0402 gene of Pseudomonas aeruginosa PAO1, Chromosome (444687 to 445691 bp)


Displaying the first five SNPs out of a total of 41 found in all isolate strains ( View all 41 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA7 Transition g.444774G>A
Silent
TAC(Tyr) -> TAT(Tyr) 		SNP-484468 View
Pseudomonas aeruginosa C3719 Transversion g.444778G>C p.Thr305Ser
 Missense
ACC(Thr) -> AGC(Ser) 		SNP-18368 View
Pseudomonas aeruginosa LESB58 Transversion g.444807C>A
Silent
GGG(Gly) -> GGT(Gly) 		SNP-96438 View
Pseudomonas aeruginosa PA14 Transversion g.444807C>A
Silent
GGG(Gly) -> GGT(Gly) 		SNP-184575 View
Pseudomonas aeruginosa PA7 Transition g.444825C>T
Silent
GAG(Glu) -> GAA(Glu) 		SNP-484469 View
Select to view SNPs in a specific strain
SNP Map
PubMed Aspartate transcarbamoylase genes of Pseudomonas putida: requirement for an inactive dihydroorotase for assembly into the dodecameric holoenzyme.
Schurr MJ, Vickrey JF, Kumar AP, Campbell AL, Cunin R, Benjamin RC, Shanley MS, O'Donovan GA.
J Bacteriol. 1995 Apr;177(7):1751-9.
PMID: 7896697
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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