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               PA0440               
Key to diagram


View in GBrowse: 11 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name   Alternate Gene Names dypA
Locus tag PA0440 (View 6 updates) Submit an Update GenBank Identifier (GI) 15595637 (Precomputed BLAST results at BLINK )
Genomic location 494721 - 493354 (-) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name probable oxidoreductase (Class 3  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA00441
Systomonas PA0440
RefSeq NP_249131.1
GI 15595637
KEGG PA0440
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
PA0439 probable oxidoreductase
PA0440 probable oxidoreductase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13196
Genomic Context
Genomic context Explanation of Genomic Context Field - With the adjacent gene, may encode a two-component oxidoreductase.
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Putative enzymes
GO Accession GO Term Evidence
Molecular Function
GO:0004355 glutamate synthase (NADPH) activity IEA
Biological Process
GO:0006537 glutamate biosynthetic process IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0493 GltD, NADPH-dependent glutamate synthase beta chain and related oxidoreductases [Amino acid transport and metabolism / General function prediction only]. Amino acid transport and metabolism / General function prediction only
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Alanine, aspartate and glutamate metabolism KEGG pae00250 Computationally Generated
Nitrogen metabolism KEGG pae00910 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 63% similar to NADPH dependent glutamate synthase small subunit homolog [Rhodobacter capsulatus]
Most Similar Human Homolog
ENSP00000377715, Percent Identity: 34.28%, E-Value: 1e-51, Ensembl Release: 56.
Description: Dihydropyrimidine dehydrogenase
Structure Features
Structure Features Explanation of Structure Features Field PR00419: Adrenodoxin reductase family signature
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF03486 HI0933_like, HI0933-like protein. 141 176 1e-05 23.0
PF00070 Pyr_redox, Pyridine nucleotide-disulphide oxidoreductase. 142 235 8e-05 23.0
PF01266 DAO, FAD dependent oxidoreductase. 142 247 9e-07 23.0
PF07992 Pyr_redox_2, Pyridine nucleotide-disulphide oxidoreductase. 142 424 9e-29 23.0
PF01593 Amino_oxidase, Flavin containing amine oxidoreductase. 151 177 5e-05 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region CATGGCGCTGCTGTGGGACGCCGGGGTCAACAGCGGGCGCCTGTCGATGCACGAGTTCGTCGCGCTGACCTCCACCAACACCGCGAAGATCTTCAACCTG TTCCCGCGCAAGGGCGCGATCCGCGTCGGCGCGGATGCCGACCTGGTGCTCTGGGACCCGCAGGGCAGCCGCACCCTCTCGGCCGCCACCCACCACCAGC GGGTCGATTTCAACATCTTCGAAGGTCGCACCGTGCGCGGCATTCCCAGCCACACCATCAGCCAGGGCAAGCTGCTCTGGGCCGCCGGCGACCTGCGCGC CGAACCCGGCGCCGGACGCTACGTGGAGCGCCCGGCCTACCCGTCGGTGTACGAAGTGCTCGGCCGCCGCGCCGAACGCCAGCGCCCGGTCGCCGTCGAG CGCTGATCCTTCCCGCAATACCGGACCGGCCCGTAGAGGCCGGACGGTGCCGTATTGCCGTTGACTGCCCCGAGAAGAAACCAGAAGAGAGGCTACAACC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence GTGATCGATTCCCTGAACCACCTCCCCCGTCCGGACGCCGGCGCGGACACGCTCGCCGAGCGCTTCGCCGACCTCGCGCCGCCGCTCAACGCCCGCCAGG CCGCCCTGGAAAGCGCCCGCTGCCTGTACTGCTACGACGCGCCCTGCGTCAACGCCTGCCCGACCGAGATCGACATCCCCGGGTTCATCCGCAGCATCGC CCAGGAGAATGTCCAGGGCGCGGCGCAGAAGATCCTCTCGGCGAACATCCTCGGCGGCAGCTGCGCCCGCGTCTGCCCCACGGAAATCCTCTGCCAGCGG GCCTGCGTACGCAACAACGCCCAGGAATGCGCACCGGTGCTGATCGGCCAGTTGCAACGCTACGCCCTCGACCACGCCGGCTTCGACGAACATCCGTTCA AGCGCTCGCCGGCCACCGGCAAGCGCATCGCCGTGGTCGGCGCCGGGCCGGCCGGGCTGTCCTGCGCCCACCGCCTGGCCATGCACGGCCACGACGTGGT GCTCTTCGAGGCCCGCACCAAGGCCGGCGGCCTCAACGAGTACGGCATCGCCCGCTACAAGCTGGTGGACGACTTCGCCCGGCGCGAAGTGGAATTCCTC CTGCAGATCGGCGGTATCGAACTGCGCCACGGCCAGCGTCTCGGCGACAACCTGGCGCTCGGCGCCCTGCACGACGAGTACGACGCGGTGTTCCTCGGCA TCGGCCTGGCCGCCAGCAAGCGCCTCGGCCTGGAAGACGAGGACGCCCCCGGCCTGCTCGCCGCCACCGACTACATCCGCGAACTGCGGCAGAGCGACGA CCTGCTCGACCTGCCCGTCGCCGACCGTTGCATCGTCATCGGCGCCGGCAACACCGCCATCGACATGGCGGTGCAGATGAGCCGGCTCGGCGCCCGCGAC GTCAACCTGGTCTACCGCCGTGGCTTCGAGGAAATGGGCGCCACCGGCCATGAGCAGGAGATCGCCAAGGCCAGCCAGGTGCGCCTGCTGACCTGGGCCC GACCGGAACGGGTACTGCTCGACGAGCACGGCCGGGTGCGCGGCATGCGCTTCGCCCGCACGCGCCTGGAGAACGGTCGCCTGGGCGACACCGGCGAGCA CTTCGAACTGGCCGCCGACGCCATCTTCAAGGCCATCGGCCAGGCCTTCGAAGGCGCCAGCCTGGGCGATCCGCTGGCAGCGCAACTGCGGCGCGACGGC GAGCGCATCCAGGTCGACGGACAGATGCGCACCAGCATCCCCGGCATCTACGCCGGCGGCGACTGCGTGGCGCTCGGCGAGGACCTCACCGTGCAGGCGG TGCAGCACGGCAAACTGGCGGCGGAAGCGATCCACGCCCAACTCATGCTCAACGTGGAGGCTGCGTGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MIDSLNHLPRPDAGADTLAERFADLAPPLNARQAALESARCLYCYDAPCVNACPTEIDIPGFIRSIAQENVQGAAQKILSANILGGSCARVCPTEILCQR ACVRNNAQECAPVLIGQLQRYALDHAGFDEHPFKRSPATGKRIAVVGAGPAGLSCAHRLAMHGHDVVLFEARTKAGGLNEYGIARYKLVDDFARREVEFL LQIGGIELRHGQRLGDNLALGALHDEYDAVFLGIGLAASKRLGLEDEDAPGLLAATDYIRELRQSDDLLDLPVADRCIVIGAGNTAIDMAVQMSRLGARD VNLVYRRGFEEMGATGHEQEIAKASQVRLLTWARPERVLLDEHGRVRGMRFARTRLENGRLGDTGEHFELAADAIFKAIGQAFEGASLGDPLAAQLRRDG ERIQVDGQMRTSIPGIYAGGDCVALGEDLTVQAVQHGKLAAEAIHAQLMLNVEAA

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0440 gene of Pseudomonas aeruginosa PAO1, Chromosome (493354 to 494721 bp)


Displaying the first five SNPs out of a total of 74 found in all isolate strains ( View all 74 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA7 Transversion g.493432G>C
Silent
ACC(Thr) -> ACG(Thr) 		SNP-486332 View
Pseudomonas aeruginosa PA7 Transversion g.493480G>C
Silent
CCC(Pro) -> CCG(Pro) 		SNP-486333 View
Pseudomonas aeruginosa PA7 Transition g.493501T>C
Silent
GGA(Gly) -> GGG(Gly) 		SNP-486334 View
Pseudomonas aeruginosa PACS2 Transversion g.493507G>C
Silent
GTC(Val) -> GTG(Val) 		SNP-104814 View
Pseudomonas aeruginosa PA7 Transversion g.493519C>G p.Glu401Gly
 Missense
GAG(Glu) -> GGC(Gly) 		SNP-486335 View
Select to view SNPs in a specific strain
SNP Map
PubMed Gene cloning, sequencing and enzymatic properties of glutamate synthase from the hyperthermophilic archaeon Pyrococcus sp. KOD1.
Jongsareejit B, Rahman RN, Fujiwara S, Imanaka T.
Mol Gen Genet. 1997 May;254(6):635-42.
PMID: 9202379
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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