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               PA0441               
Key to diagram


View in GBrowse: 10 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name dht  Alternate Gene Names hyuA
Locus tag PA0441 (View 7 updates) Submit an Update GenBank Identifier (GI) 15595638 (Precomputed BLAST results at BLINK )
Genomic location 496255 - 494816 (-) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name dihydropyrimidinase (Class 2  Explanation of Product Name Confidence)
Alternate Product Names D-hydantoinase, 5,6-dihydropyrimidine amidohydrolase, (DHPase)
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA00442
Systomonas PA0441
RefSeq NP_249132.1
GI 15595638
KEGG PA0441
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Nucleotide biosynthesis and metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0004157 dihydropyrimidinase activity IEA
Biological Process
GO:0009117 nucleotide metabolic process ISS
GO:0008152 metabolic process IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0044 PyrC, Dihydroorotase and related cyclic amidohydrolases [Nucleotide transport and metabolism]. Nucleotide transport and metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Pyrimidine metabolism KEGG pae00240 Computationally Generated
beta-Alanine metabolism KEGG pae00410 Computationally Generated
Pantothenate and CoA biosynthesis KEGG pae00770 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 3.5.2.2
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 95% similar to D-hydantoinase [Pseudomonas putida]
Most Similar Human Homolog
ENSP00000276651, Percent Identity: 51.24%, E-Value: 2e-129, Ensembl Release: 56.
Description: Dihydropyrimidinase
Structure Features
Structure Features Explanation of Structure Features Field PF00744: Dihydooratase-like
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF01979 Amidohydro_1, Amidohydrolase family. 50 397 2e-14 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region ATAAGCGTGCCGAAAACCCCGCGACGTCACTGGGCAGGCCGGTCTACTGGAGGTAACCGACTGATCTGAAACACTTCATCGACAACGTCGGTGACACGCC GACGGGCAGTCCGGCGTGTCACCGGGGGAAATGCGCGAGCCTGTCGCCCCGGTCAGGAACCAAAGGAGTGCAGGCGAACTTTTTTCATCTCCCCACAACC GTTCGAACACGGCTTTCAAGCGGCTGATTTTGCATGGGAAATCTCGACCTTTTTGAATTCTTGTCAAGCGCGTCAAAATGGTGAGAACCCTCACCATTTT GGTGATATTTATTTTTAATTATTTAAAATCAATAAGTTACAAACTCAAACAACCATTTGAAAACAATCTTGCTCAATCGATGATCAGCATCTAGATTCGA ATCCTGTCAGCGCTGACAGGATTACAAAAGTCCTCGGCGCATGGCGAAAAAACAATTTCAAGAACCGGCAACGACCGGTCAGCCTGCGAGGAAAGACGGC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACTCACGAAGAGAGTTATCGAGCCGATGTGCTCTGTGCCAACGGCCTCATCCAGGCCATCGGCGAGA ACCTCGAAACCCCCTCCGGCTGCGACGTCCTCGACGGCGGTGGCCAGTACCTGATGCCGGGCGGCATCGACCCCCATACCCACATGCAGTTGCCCTTCAT GGGCACGGTGGCCAGCGAGGACTTCTTCAGCGGCACCGCGGCCGGGCTGGCCGGAGGGACCACCTCGATCATCGACTTCGTCATCCCCAACCCGCGACAG TCGCTGCTGGAGGCCTTCCACACCTGGCGCGGCTGGGCGCAGAAATCCGCTGCGGACTACGGCTTCCACGTCGCCATCACCTGGTGGAGCGACGAGGTCG CCCGGGAAATGGGCGAGCTGGTGGCACAGCACGGGGTGAACAGCTTCAAGCACTTCATGGCCTACAAGAACGCCATCATGGCCGCCGACGATACCCTGGT GGCCAGCTTCGAGCGCTGCCTGGAGCTGGGCGCGGTGCCGACGGTGCACGCGGAGAACGGCGAACTGGTCTTCCACCTGCAGCAGAAACTCCTCGCCCAG GGCCTCACCGGCCCCGAGGCGCATCCTCTGTCGCGTCCGCCGCAAGTCGAGGGCGAGGCCGCCAGCCGCGCCATCCGCATAGCCGAGACGCTGGGTACGC CGCTGTACCTGGTACATATTTCCAGCCGCGAGGCGCTGGACGAGATCGCCTATGCCCGCGCCAAGGGCCAGCCGGTCTACGGCGAGGTGCTGGCCGGGCA CCTGCTGCTCGACGACAGCGTCTACCGCCACCCGGACTGGGCCACCGCGGCCGGCTACGTGATGAGCCCGCCGTTCCGTCCCGTCGAACACCAGGAAGCG CTGTGGCGCGGCCTGCAGTCCGGCAACCTGCATACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAGGCCATGGGCCGCGACGACTTCAGCA AGATTCCCAATGGCACGGCCGGCATCGAGGACCGCATGGCGCTGCTGTGGGACGCCGGGGTCAACAGCGGGCGCCTGTCGATGCACGAGTTCGTCGCGCT GACCTCCACCAACACCGCGAAGATCTTCAACCTGTTCCCGCGCAAGGGCGCGATCCGCGTCGGCGCGGATGCCGACCTGGTGCTCTGGGACCCGCAGGGC AGCCGCACCCTCTCGGCCGCCACCCACCACCAGCGGGTCGATTTCAACATCTTCGAAGGTCGCACCGTGCGCGGCATTCCCAGCCACACCATCAGCCAGG GCAAGCTGCTCTGGGCCGCCGGCGACCTGCGCGCCGAACCCGGCGCCGGACGCTACGTGGAGCGCCCGGCCTACCCGTCGGTGTACGAAGTGCTCGGCCG CCGCGCCGAACGCCAGCGCCCGGTCGCCGTCGAGCGCTGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MSLLIRGATVVTHEESYRADVLCANGLIQAIGENLETPSGCDVLDGGGQYLMPGGIDPHTHMQLPFMGTVASEDFFSGTAAGLAGGTTSIIDFVIPNPRQ SLLEAFHTWRGWAQKSAADYGFHVAITWWSDEVAREMGELVAQHGVNSFKHFMAYKNAIMAADDTLVASFERCLELGAVPTVHAENGELVFHLQQKLLAQ GLTGPEAHPLSRPPQVEGEAASRAIRIAETLGTPLYLVHISSREALDEIAYARAKGQPVYGEVLAGHLLLDDSVYRHPDWATAAGYVMSPPFRPVEHQEA LWRGLQSGNLHTTATDHCCFCAEQKAMGRDDFSKIPNGTAGIEDRMALLWDAGVNSGRLSMHEFVALTSTNTAKIFNLFPRKGAIRVGADADLVLWDPQG SRTLSAATHHQRVDFNIFEGRTVRGIPSHTISQGKLLWAAGDLRAEPGAGRYVERPAYPSVYEVLGRRAERQRPVAVER

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0441 gene of Pseudomonas aeruginosa PAO1, Chromosome (494816 to 496255 bp)


Displaying the first five SNPs out of a total of 77 found in all isolate strains ( View all 77 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa C3719 Transversion g.494936G>T
Silent
GCC(Ala) -> GCA(Ala) 		SNP-18543 View
Pseudomonas aeruginosa 2192 Transversion g.494936G>T
Silent
GCC(Ala) -> GCA(Ala) 		SNP-67832 View
Pseudomonas aeruginosa PACS2 Transversion g.494936G>T
Silent
GCC(Ala) -> GCA(Ala) 		SNP-104820 View
Pseudomonas aeruginosa C3719 Transversion g.494978A>T
Silent
ATT(Ile) -> ATA(Ile) 		SNP-18544 View
Pseudomonas aeruginosa 2192 Transversion g.494978A>T
Silent
ATT(Ile) -> ATA(Ile) 		SNP-67833 View
Select to view SNPs in a specific strain
SNP Map
PubMed Pyrimidine catabolism in Pseudomonas aeruginosa.
Kim S, West TP.
FEMS Microbiol Lett. 1991 Jan 15;61(2-3):175-9.
PMID: 1903745
Cloning, sequencing, and expression in Escherichia coli of the D-hydantoinase gene from Pseudomonas putida and distribution of homologous genes in other microorganisms.
LaPointe G, Viau S, LeBlanc D, Robert N, Morin A.
Appl Environ Microbiol. 1994 Mar;60(3):888-95.
PMID: 8161181
Identification of the open reading frame for the Pseudomonas putida D-hydantoinase gene and expression of the gene in Escherichia coli.
Chien HR, Jih YL, Yang WY, Hsu WH.
Biochim Biophys Acta. 1998 Jan 7;1395(1):68-77.
PMID: 9434154
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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