Pseudomonas Genome Database - Annotation for PA0444

Add this annotation to your clipboard

PA0444

Key to diagram

View in GBrowse:

15 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Operons/Regulons
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name		Alternate Gene Names	hyuC
Locus tag	PA0444 (View 6 updates) Submit an Update	GenBank Identifier (GI)	15595641 (Precomputed BLAST results at BLINK )
Genomic location	498420 - 499703 (+)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG000435 Orthology uncertain because more than one top BLASTp hit was identified.
Product Information
Product Name	N-carbamoyl-beta-alanine amidohydrolase (Class 2 )
Alternate Product Names	hydantoinase
Subcellular localization	Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA00445
Systomonas	PA0444
RefSeq	NP_249135.1
GI	15595641
KEGG	PA0444
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Operons/Regulons

Type

operon

Genes

Gene	Product
PA0443	probable transporter
PA0444	N-carbamoyl-beta-alanine amidohydrolase

Evidence

Computationally Predicted

PubMed

18988623

Cross-Reference

DOOR-13197

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Nucleotide biosynthesis and metabolism

GO Accession

GO Term

Evidence

Molecular Function

GO:0008233

peptidase activity

RCA

Biological Process

GO:0009117	nucleotide metabolic process	ISS
GO:0006508	proteolysis	RCA

Cellular Component

COG Prediction

Accession	Description	Category
COG0624	ArgE, Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases [Amino acid transport and metabolism].	Amino acid transport and metabolism

Pathways

Name	Database	Xref
Pyrimidine metabolism	PseudoCAP

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

55% similar to DL-hydantoinase (N-carbamyl-L-amino acid amidohydrolase) [Pseudomonas sp.]

Structure Features

PD00794: HYDROLASE PROTEIN DIPEPTIDASE BIOSYNTHESIS AMIDOHYDROLASE ZINC ACID COBALT CARBOXYPEPTIDASE METALLOPROTEASE

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF01546	Peptidase_M20, Peptidase family M20/M25/M40.	88	415	6e-33	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

ACTCATCGGCCGCACCCGCGCCGTCTGGCTCACCGGTTTCATCGGCCTGGCGCTGATGGCCCACGAGTTGCTGAAGAAGCTCGGCTGGATCGTTTCCGAC CTCAGCCTGGAAAGCGTCTACTCCAACTGGCTGCTCGGCTACTCCAGCCTGCTCGGGCCGATCGCGGGGATCATGGTGGTGGACTACTTCCTGGTGCGCA GGCAACGCCTCGATCTCGCCGGACTCTACCGCGACGACGTCTACCCGGCCTGGAACTGGCCCGGCTTCGCCGCCTTCGCGGTGCCGGTGGCGCTGACCGT CATGGCCCTCGGCAACCGCCAGTTCCACTGGTTCTACGACTACGGCTGGTTCACCGGCTCGCTGCTCGGCGGCGCGCTCTACTACGGCCTCTGCGGCCGC CGTGCGCGGAGCCCGGCGGCATTGGCCAAACCGCTGCCCTGATGGGCATACAGGCGGCCGGCGAGCCGCCGACACCTACTCCAAGCCTGAGGAGACGACA

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGAGCACTGCCCGGAACGTCCTGCAATCCACCCAGCGACACATCGATGGCCAGCGCCTCTGGCAATCGCTGATGGATCTCGCCCGCCTCGGCGCCACCG CCAAGGGTGGGGTCTGCCGCCTGGCCCTGAGCGATCTCGACCGCCAGGCCCGCGATCTCTTCGTGCAATGGTGCGAAGCGGCCGGCTGCACGGTCAGCGT CGATCGGGTCGGCAACATCTTCGCCCGTCGTCCCGGGCGTAACCCGGACTTGCCCCCGGTGATGACCGGTAGCCACATCGACACCCAGCCCACCGGTGGC AAGTTCGACGGCTGCTTCGGGGTGATGGCCGGCCTCGAGGTGATCCGCACCCTCAACGACCTCGGGGTGGAAACCGAGGCGCCGCTGGAGGTGGTGGTGT GGACCAACGAGGAAGGCTCGCGCTTCGCGCCCTGCATGATGGGCTCGGGCGTATTCGCCGGGAAGTTCACCCTGGAGGAGACCCTGGCCAAGCGCGATGC CGACGGTGTCAGCGTAGGCGAGGCGCTGGACGCCATCGGCTACGCGGGAGCGCGCGATTGTCTCGGACATCCGGTGGGCGCCTATTTCGAGGCGCACATC GAACAGGGGCCGATCCTCGAGGACGAGGAGAAGACCATCGGCGTGGTGCTCGGCGCGCTCGGGCAGAAATGGTTCGACCTGTCCCTGCGCGGCGTCGAGG CACACGCCGGGCCAACGCCGATGCACCTGCGCAAGGATGCCCTGGTCGGTGCCGCGGCGGTGGTCGAGGCGGTCAATCGCGCGGCCCTCGGCCATCAGCC GCATGCTTGCGGCACGGTCGGCTGCCTGCACGCCTATCCCGGTTCGCGCAACGTGATACCCGGCGAAGTGAAGATGACCCTGGACTTCCGCCATCTGCAA CCGGAGCGCCTGGACTCGATGATCGCCGAGGTCCGCCAGGTGATCGCCGCTACCTGCGAGAAGCATGGCTTGCAATACGAGCTGGTGCCGACCGCCGATT TCCCGCCGCTGTACTTCGACCAGGGATGCGTCGGCGCGGTGCGCGAGGCGGCGCAGGCGCTGGGCATGCCGCAGATGGACATCGTCAGCGGCGCCGGCCA CGACGCGATCTTCCTCGCCGAACTCGGTCCGGCGGGGATGATCTTCGTGCCCTGCGAGAACGGCATCAGCCACAACGAGATCGAGAACGCCAGCCCCGAC GACCTGGCCGCCGGCTGCGCGGTGCTGTTGCGGGCCATGCTCAAGGCCTCCGAGGCGATCGCCGGCGGCCGCCTGGCGGCCTGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MSTARNVLQSTQRHIDGQRLWQSLMDLARLGATAKGGVCRLALSDLDRQARDLFVQWCEAAGCTVSVDRVGNIFARRPGRNPDLPPVMTGSHIDTQPTGG KFDGCFGVMAGLEVIRTLNDLGVETEAPLEVVVWTNEEGSRFAPCMMGSGVFAGKFTLEETLAKRDADGVSVGEALDAIGYAGARDCLGHPVGAYFEAHI EQGPILEDEEKTIGVVLGALGQKWFDLSLRGVEAHAGPTPMHLRKDALVGAAAVVEAVNRAALGHQPHACGTVGCLHAYPGSRNVIPGEVKMTLDFRHLQ PERLDSMIAEVRQVIAATCEKHGLQYELVPTADFPPLYFDQGCVGAVREAAQALGMPQMDIVSGAGHDAIFLAELGPAGMIFVPCENGISHNEIENASPD DLAAGCAVLLRAMLKASEAIAGGRLAA

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0444 gene of Pseudomonas aeruginosa PAO1, Chromosome (498420 to 499703 bp)

Displaying the first five SNPs out of a total of 50 found in all isolate strains ( View all 50 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa LESB58	Transition	g.498430C>T	p.Ala4Val	Missense GCC(Ala) -> GTC(Val)	SNP-96615	View
Pseudomonas aeruginosa PA7	Transition	g.498458A>G		Silent CGA(Arg) -> CGG(Arg)	SNP-486489	View
Pseudomonas aeruginosa PA7	Transition	g.498476C>T		Silent CGC(Arg) -> CGT(Arg)	SNP-486490	View
Pseudomonas aeruginosa PA7	Transition	g.498527T>C		Silent GGT(Gly) -> GGC(Gly)	SNP-486491	View
Pseudomonas aeruginosa PA7	Transversion	g.498550G>C	p.Ser44Thr	Missense AGC(Ser) -> ACC(Thr)	SNP-486492	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Pyrimidine catabolism in Pseudomonas aeruginosa.
Kim S, West TP.
FEMS Microbiol Lett. 1991 Jan 15;61(2-3):175-9.
PMID: 1903745
Cloning and sequencing of the genes involved in the conversion of 5-substituted hydantoins to the corresponding L-amino acids from the native plasmid of Pseudomonas sp. strain NS671.
Watabe K, Ishikawa T, Mukohara Y, Nakamura H.
J Bacteriol. 1992 Feb;174(3):962-9.
PMID: 1732229