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               PA0500               
Key to diagram


View in GBrowse: 16 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name bioB  Alternate Gene Names
Locus tag PA0500 (View 8 updates) Submit an Update GenBank Identifier (GI) 15595697 (Precomputed BLAST results at BLINK )
Genomic location 559644 - 560702 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name biotin synthase (Class 2  Explanation of Product Name Confidence)
Alternate Product Names biotin synthetase
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA00501
Systomonas PA0500
RefSeq NP_249191.1
GI 15595697
KEGG PA0500
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
bioB biotin synthase
bioF 8-amino-7-oxononanoate synthase
PA0502 probable biotin biosynthesis protein bioH
PA0503 probable biotin synthesis protein BioC
bioD dethiobiotin synthase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13212
Genomic Context
Genomic context Explanation of Genomic Context Field - Upstream of the bioFHCD genes
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Biosynthesis of cofactors, prosthetic groups and carriers
GO Accession GO Term Evidence
Molecular Function
GO:0004076 biotin synthase activity IEA
Biological Process
GO:0051188 cofactor biosynthetic process ISS
GO:0009102 biotin biosynthetic process IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0502 BioB, Biotin synthase and related enzymes [Coenzyme metabolism]. Coenzyme metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Biotin metabolism PseudoCAP
biotin biosynthesis II PseudoCyc BIOTIN-SYNTHESIS-II-PWY
Biotin metabolism KEGG pae00780 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 2.8.1.6
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 82% similar to BIOTIN SYNTHASE [Escherichia coli]
Structure Features
Structure Features Explanation of Structure Features Field DM01585: SYNTHETASE BIOTIN
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF04055 Radical_SAM, Radical SAM superfamily. 54 212 2e-19 23.0
PF06968 BATS, Biotin and Thiamin Synthesis associated domain. 226 318 4e-32 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region ACCCGTATCGTGTTTTGGAAAGTGGTTGGTCGGAAGGGTCGAATATATTTTTCGTTATACTTCCGCTGCGCTTTGAATGGCTATATTTAGGGTGGTTGGT TGCGTTCATGGTTCAAGTCTGGATAACTTGTTTTCGTTGCGCACCGCTAGGGTGGCAAGAGATTCAATGTTTCTCCATGTTAGAAACCTGCCAGGGAATA TATAAATAAGATATTTCCTATTTCTTGTAGGAAATATCCTAAGCATTACTTGTAGTTTTCCGAGGTAGTTGCGCCGTTTCTTCTAGGGAGCCGAATGCCA CATAGAGGACGAGGCCGATTGGAGCAGTCGATGACTACTGGCTTCTACATGAATGATCAAATATTGATCAGATGTAGTCCTGTGTCCTGGGTTGGGTTGA CAGCGTCATACAGCCACTTATGATGCCCTTGAGCCTGACCCCCGGCGTGCCGGGGCTCCCCGACAAAAGGCGCCATACAAGCGTCGAAGGAATCTTCCCC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGAGTGCAACCGCTTCCGTCGCCACCCGTCACGACTGGTCCCTCGCCGAAGTCCGTGCCCTGTTCGAGCAACCGTTCAACGACCTGCTGTTCCAGGCGC AGACGGTGCATCGCGCGCACTTCGACCCGAACCGCGTGCAGGTCTCGACGCTGCTGTCGATCAAGACCGGCGCCTGCCCCGAGGACTGCAAGTACTGCCC GCAGTCCGGCCACTACAACACCGGCCTGGACAAGGAGAAGCTGATGGAGGTGCAGAAGGTCCTCGAGGCGGCGGCCGAGGCCAAGGCCATCGGTTCGACC CGCTTCTGCATGGGCGCTGCCTGGAAGCACCCGTCGGCCAAGGACATGCCCTACGTCCTGGAGATGGTCAAGGGCGTGAAGAAGCTCGGCCTGGAGACCT GCATGACCCTCGGTCGCCTGACCCAGGAACAGACCCAGGCGCTGGCGGACGCCGGCCTCGACTACTACAACCACAACCTGGATACCTCGCCGGAGTTCTA CGGCAACATCATCACCACCCGCACCTACAGCGAGCGCCTGCAGACCCTGGCCTACGTGCGCGAGGCGGGGATGAAGATCTGCTCCGGCGGCATCCTCGGC ATGGGCGAGTCGGTGGACGACCGCGCCGGCCTGCTGATCCAACTGGCGAACCTGCCGGAGCACCCGGAGTCGGTGCCGATCAACATGCTGGTGAAGGTCA AGGGCACGCCCCTGGCGGAAGAGAAGGACGTCGATCCCTTCGACTTCATCCGCACCCTGGCGGTGGCCCGGATCATGATGCCGAAGTCCCACGTGCGCCT GTCCGCCGGCCGCGAGCAGATGAACGAGCAGATGCAGGCGCTGGCCTTCATGGCCGGGGCCAACTCGATCTTCTACGGCGAAAAGCTGCTGACCACGAAG AACCCGCAGGCCGAAAAGGACATGCAGTTGTTCGCCCGTCTCGGCATCAAGCCGGAAGAGCGCGAAGAGCACGCCGACGAAGTGCACCAGGCCGCCATCG AGCAGGCGTTGGTGGAACAACGCGAATCGAAGCTGTTCTATAACGCCGCTTCCGCCTGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MSATASVATRHDWSLAEVRALFEQPFNDLLFQAQTVHRAHFDPNRVQVSTLLSIKTGACPEDCKYCPQSGHYNTGLDKEKLMEVQKVLEAAAEAKAIGST RFCMGAAWKHPSAKDMPYVLEMVKGVKKLGLETCMTLGRLTQEQTQALADAGLDYYNHNLDTSPEFYGNIITTRTYSERLQTLAYVREAGMKICSGGILG MGESVDDRAGLLIQLANLPEHPESVPINMLVKVKGTPLAEEKDVDPFDFIRTLAVARIMMPKSHVRLSAGREQMNEQMQALAFMAGANSIFYGEKLLTTK NPQAEKDMQLFARLGIKPEEREEHADEVHQAAIEQALVEQRESKLFYNAASA

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0500 gene of Pseudomonas aeruginosa PAO1, Chromosome (559644 to 560702 bp)


Displaying the first five SNPs out of a total of 61 found in all isolate strains ( View all 61 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA7 Transition g.559649T>C
Silent
AGT(Ser) -> AGC(Ser) 		SNP-191457 View
Pseudomonas aeruginosa PA7 Transversion g.559653A>T p.Thr4Ser
 Missense
ACC(Thr) -> TCC(Ser) 		SNP-191458 View
Pseudomonas aeruginosa PA7 Transversion g.559658T>A
Silent
GCT(Ala) -> GCA(Ala) 		SNP-191459 View
Pseudomonas aeruginosa PA7 Transversion g.559659T>G p.Ser6Ala
 Missense
TCC(Ser) -> GCC(Ala) 		SNP-191460 View
Pseudomonas aeruginosa PA7 Transition g.559673T>C
Silent
CGT(Arg) -> CGC(Arg) 		SNP-191461 View
Select to view SNPs in a specific strain
SNP Map
PubMed Biotin synthase: purification, characterization as a [2Fe-2S]cluster protein, and in vitro activity of the Escherichia coli bioB gene product.
Sanyal I, Cohen G, Flint DH.
Biochemistry. 1994 Mar 29;33(12):3625-31.
PMID: 8142361
Biotin synthase from Escherichia coli: isolation of an enzyme-generated intermediate and stoichiometry of S-adenosylmethionine use.
Shaw NM, Birch OM, Tinschert A, Venetz V, Dietrich R, Savoy LA.
Biochem J. 1998 Mar 15;330 ( Pt 3):1079-85.
PMID: 9494071
Transfer of sulfur to biotin from biotin synthase (BioB protein).
Gibson KJ, Pelletier DA, Turner IM Sr.
Biochem Biophys Res Commun. 1999 Jan 27;254(3):632-5.
PMID: 9920791
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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