Pseudomonas Genome Database - Annotation for PA0523

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PA0523

Key to diagram

View in GBrowse:

4 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Operons/Regulons
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name	norC	Alternate Gene Names
Locus tag	PA0523 (View 7 updates) Submit an Update	GenBank Identifier (GI)	15595720 (Precomputed BLAST results at BLINK )
Genomic location	582015 - 582455 (+)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG000507
Product Information
Product Name	nitric-oxide reductase subunit C (Class 1 )
Alternate Product Names	cytochrome c subunit of nitric oxide reductase
Subcellular localization	Cytoplasmic Membrane (Class 1) Cytoplasmic Membrane (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA00524
Systomonas	PA0523
RefSeq	NP_249214.1
GI	15595720
KEGG	PA0523
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Operons/Regulons

Type

operon

Genes

Gene	Product
norC	nitric-oxide reductase subunit C
norB	nitric-oxide reductase subunit B
PA0525	probable dinitrification protein NorD

Evidence

Computationally Predicted

PubMed

18988623

Cross-Reference

DOOR-13215

Genomic Context

Genomic context

- norCB gene cluster

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Energy metabolism

GO Accession

GO Term

Evidence

Molecular Function

Biological Process

GO:0006091

generation of precursor metabolites and energy

ISS

Cellular Component

Pathways

Name	Database	Xref
Denitrification	PseudoCAP
denitrification pathway	PseudoCyc	DENITRIFICATION-PWY
Nitrogen metabolism	KEGG	pae00910	Computationally Generated

Reactions

EC Number

1.7.99.7

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

100% identical to cytochrome c subunit of nitric oxide reductase [Pseudomonas aeruginosa]

Structure Features

1 predicted transmembrane helices (TMHMM v.2.0)
At least one transmembrane helix predicted by Phobius

PFAM predictions

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

TGATCCTCGGCTGGCTGGCGCAGGCCTGCTGGCGCCGGTGTTATACGCCGCAGGCTTGCTCGGGACTGGAGTCGGGAGTGCTCTACTGGCACATGGTCGA CCTGGTCTGGGTGCTGCTGTTCCCGCTGGTCTACGTCCTGCAATGAGGTGCCGATGCGAACCCTGACCCTCTGCTGGCTGGCGTTGCTGGCGCTGGCCGT GACCGGTGTGCTGCTGGGTGGCGCCGGCGACTCTCCCTGGCTGCTGGCGGCGGTGCTGGCCTGCGCGGTGGCCAAGGGTTGGCTGATCGGCGAGCGCTTC ATGGAGCTGGCCCATGCCCCGGCGCTATGGCGCCGCCTGCTGCTGGCCTGGCCGCTGCTGATGGCCCTGGCGGTGGGCGCTGCGCTGTACCTGGCGCGGA TGAATAACTGAGTAAATCGTTCGGGAATCTTGATTGCCATCAAGCGGGTTCGCCGCCGCCGCTTCCTAGAATCGCCGCACCACCCATTCCAGGAGGCCGC

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGTCCGAGACCTTTACCAAAGGCATGGCCAGGAACATCTATTTCGGCGGGAGTGTGTTCTTCATCCTGCTGTTCCTGGCCCTGACCTACCACACCGAGA AGACCCTGCCCGAGCGCACCAACGAGGCCGCGATGAGCGCCGCGGTGGTGCGCGGCAAGCTGGTCTGGGAACAGAACAACTGCGTCGGCTGCCACACCCT GCTCGGCGAGGGCGCCTACTTCGCGCCGGAGCTGGGCAACGTGGTCGGACGCCGTGGCGGCGAGGAGGGCTTCAACACCTTCCTCCAGGCCTGGATGAAT ATCCAGCCGCTGAACGTGCCGGGCCGCCGCGCCATGCCGCAGTTCCATCTCAGCGAGGGCCAGGTCGACGACCTGGCCGAGTTCCTCAAGTGGAGCTCGA AGATCGATACCAACCAATGGCCGCCGAACAAGGAGGGTTGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MSETFTKGMARNIYFGGSVFFILLFLALTYHTEKTLPERTNEAAMSAAVVRGKLVWEQNNCVGCHTLLGEGAYFAPELGNVVGRRGGEEGFNTFLQAWMN IQPLNVPGRRAMPQFHLSEGQVDDLAEFLKWSSKIDTNQWPPNKEG

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0523 gene of Pseudomonas aeruginosa PAO1, Chromosome (582015 to 582455 bp)

Displaying the first five SNPs out of a total of 18 found in all isolate strains ( View all 18 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa C3719	Indel	g.582057_582058dupT		1 BP Duplication (Frameshift)	SNP-7359	View
Pseudomonas aeruginosa PA7	Transition	g.582084C>T		Silent CTG(Leu) -> TTG(Leu)	SNP-192223	View
Pseudomonas aeruginosa PA7	Transversion	g.582154C>A	p.Ala47Glu	Missense GCC(Ala) -> GAA(Glu)	SNP-192224	View
Pseudomonas aeruginosa PA7	Transversion	g.582155C>A	p.Ala47Glu	Missense GCC(Ala) -> GAA(Glu)	SNP-192225	View
Pseudomonas aeruginosa LESB58	Transition	g.582167C>T		Silent CGC(Arg) -> CGT(Arg)	SNP-73448	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE.
Genome Res. 2005 Feb;15(2):321-9.
PMID: 15687295
Purification and characterization of nitrous oxide reductase from Pseudomonas aeruginosa strain P2.
SooHoo CK, Hollocher TC.
J Biol Chem. 1991 Feb 5;266(4):2203-9.
PMID: 1899237
Extended x-ray absorption fine structure and electron paramagnetic resonance of nitrous oxide reductase from Pseudomonas aeruginosa strain P2.
SooHoo CK, Hollocher TC, Kolodziej AF, Orme-Johnson WH, Bunker G.
J Biol Chem. 1991 Feb 5;266(4):2210-8.
PMID: 1846618
The structural genes for nitric oxide reductase from Pseudomonas aeruginosa.
Arai H, Igarashi Y, Kodama T.
Biochim Biophys Acta. 1995 Apr 4;1261(2):279-84.
PMID: 7711073