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               PA0524               
Key to diagram


View in GBrowse: 4 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name norB  Alternate Gene Names
Locus tag PA0524 (View 10 updates) Submit an Update GenBank Identifier (GI) 15595721 (Precomputed BLAST results at BLINK )
Genomic location 582455 - 583855 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name nitric-oxide reductase subunit B (Class 1  Explanation of Product Name Confidence)
Alternate Product Names cytochrome B subunit of nitric oxide reductase
Subcellular localization
Alternate Annotations and Cross-References
TIGR NTL03PA00525
Systomonas PA0524
RefSeq NP_249215.1
GI 15595721
KEGG PA0524
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
norC nitric-oxide reductase subunit C
norB nitric-oxide reductase subunit B
PA0525 probable dinitrification protein NorD
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13215
Genomic Context
Genomic context Explanation of Genomic Context Field - norCB gene cluster
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Energy metabolism
GO Accession GO Term Evidence
Molecular Function
Biological Process
GO:0006091 generation of precursor metabolites and energy ISS
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG3256 NorB, Nitric oxide reductase large subunit [Inorganic ion transport and metabolism]. Inorganic ion transport and metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Denitrification PseudoCAP
denitrification pathway PseudoCyc DENITRIFICATION-PWY
Nitrogen metabolism KEGG pae00910 Computationally Generated
Reactions
EC Number 1.7.99.7
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 100% identical to cytochrome b subunit of nitric oxide reductase [Pseudomonas aeruginosa]
Structure Features
Structure Features Explanation of Structure Features Field PS00077: Heme-copper oxidase catalytic subunit, copper B binding region signature
12 predicted transmembrane helices (TMHMM v.2.0)
At least one transmembrane helix predicted by Phobius
Export across inner membrane confirmed by PhoA fusion screen
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00115 COX1, Cytochrome C and Quinol oxidase polypeptide I. 20 411 2e-05 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region CAAGCGGGTTCGCCGCCGCCGCTTCCTAGAATCGCCGCACCACCCATTCCAGGAGGCCGCATGTCCGAGACCTTTACCAAAGGCATGGCCAGGAACATCT ATTTCGGCGGGAGTGTGTTCTTCATCCTGCTGTTCCTGGCCCTGACCTACCACACCGAGAAGACCCTGCCCGAGCGCACCAACGAGGCCGCGATGAGCGC CGCGGTGGTGCGCGGCAAGCTGGTCTGGGAACAGAACAACTGCGTCGGCTGCCACACCCTGCTCGGCGAGGGCGCCTACTTCGCGCCGGAGCTGGGCAAC GTGGTCGGACGCCGTGGCGGCGAGGAGGGCTTCAACACCTTCCTCCAGGCCTGGATGAATATCCAGCCGCTGAACGTGCCGGGCCGCCGCGCCATGCCGC AGTTCCATCTCAGCGAGGGCCAGGTCGACGACCTGGCCGAGTTCCTCAAGTGGAGCTCGAAGATCGATACCAACCAATGGCCGCCGAACAAGGAGGGTTG

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGATGTCGCCCAATGGCTCCCTGAAATTCGCCTCGCAGGCGGTCGCCAAACCCTACTTCGTGTTCGCCCTGATCCTCTTCGTCGGGCAGATCCTGTTCG GCCTGATCATGGGCCTGCAGTACGTGGTCGGCGACTTCCTGTTCCCGGCGATCCCCTTCAACGTCGCGCGCATGGTGCACACCAACCTGCTGATCGTCTG GCTGCTGTTCGGCTTCATGGGCGCTGCCTACTACCTGGTGCCGGAGGAGAGCGACTGCGAGCTGTACAGCCCGAAGCTGGCCTGGATCCTGTTCTGGGTA TTCGCCGCCGCCGGCGTGCTGACCATCCTCGGCTACCTGCTGGTGCCCTACGCCGGCCTGGCCAGGCTCACCGGCAACGAACTGTGGCCGACCATGGGCC GCGAGTTCCTCGAACAGCCGACCATCAGCAAGGCGGGCATCGTGATCGTCGCCCTGGGCTTCCTGTTCAACGTCGGCATGACCGTGCTGCGCGGGCGCAA GACCGCCATCAGCATGGTCCTGATGACCGGCCTGATCGGCCTCGCCCTGCTGTTCCTGTTCTCCTTCTACAACCCGGAAAACCTCACCCGCGACAAGTTC TACTGGTGGTGGGTGGTGCACCTGTGGGTGGAAGGCGTGTGGGAACTGATCATGGGTGCGATCCTCGCCTTCGTGCTGGTGAAGATCACCGGGGTCGACC GCGAGGTGATCGAGAAGTGGCTCTACGTGATCATCGCCATGGCGCTGATCAGCGGCATCATCGGCACCGGCCACCACTACTTCTGGATCGGCGTGCCCGG CTACTGGCTGTGGCTCGGCTCGGTATTCTCGGCGCTGGAGCCGCTGCCGTTCTTCGCCATGGTGCTGTTCGCCTTCAACACCATCAACCGCCGCCGCCGC CGCGACTATCCGAACCGCGCGGTCGCGCTGTGGGCCATGGGCACCACAGTGATGGCCTTCCTCGGCGCGGGAGTGTGGGGCTTCATGCATACCCTGGCGC CGGTCAACTACTACACCCACGGCACCCAGCTCACCGCCGCCCACGGCCACATGGCCTTCTATGGCGCCTACGCGATGATCGTGATGACCATCATCTCCTA CGCCATGCCGCGCCTGCGCGGGATCGGCGAGGCGATGGACAACCGCTCGCAGGTCCTGGAGATGTGGGGCTTCTGGCTGATGACCGTGGCGATGGTGTTC ATCACCCTGTTCCTCTCCGCGGCCGGGGTGCTGCAGGTCTGGCTGCAACGGATGCCGGCGGACGGCGCGGCGATGACCTTCATGGCCACCCAGGACCAGC TGGCGATCTTCTACTGGCTGCGCGAAGGCGCCGGGGTGGTGTTCCTCATCGGCCTGGTCGCCTACCTGCTGAGCTTCCGGCGCGGCAAGGCGGCCGCCTG A

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MMSPNGSLKFASQAVAKPYFVFALILFVGQILFGLIMGLQYVVGDFLFPAIPFNVARMVHTNLLIVWLLFGFMGAAYYLVPEESDCELYSPKLAWILFWV FAAAGVLTILGYLLVPYAGLARLTGNELWPTMGREFLEQPTISKAGIVIVALGFLFNVGMTVLRGRKTAISMVLMTGLIGLALLFLFSFYNPENLTRDKF YWWWVVHLWVEGVWELIMGAILAFVLVKITGVDREVIEKWLYVIIAMALISGIIGTGHHYFWIGVPGYWLWLGSVFSALEPLPFFAMVLFAFNTINRRRR RDYPNRAVALWAMGTTVMAFLGAGVWGFMHTLAPVNYYTHGTQLTAAHGHMAFYGAYAMIVMTIISYAMPRLRGIGEAMDNRSQVLEMWGFWLMTVAMVF ITLFLSAAGVLQVWLQRMPADGAAMTFMATQDQLAIFYWLREGAGVVFLIGLVAYLLSFRRGKAAA

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0524 gene of Pseudomonas aeruginosa PAO1, Chromosome (582455 to 583855 bp)


Displaying the first five SNPs out of a total of 32 found in all isolate strains ( View all 32 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA7 Transition g.582527A>G p.Ile25Val
 Missense
ATC(Ile) -> GTC(Val) 		SNP-192232 View
Pseudomonas aeruginosa PA7 Transition g.582589C>T
Silent
GAC(Asp) -> GAT(Asp) 		SNP-192233 View
Pseudomonas aeruginosa PA7 Transition g.582712C>T
Silent
TGC(Cys) -> TGT(Cys) 		SNP-192234 View
Pseudomonas aeruginosa PA7 Transversion g.582727G>C
Silent
CCG(Pro) -> CCC(Pro) 		SNP-192235 View
Pseudomonas aeruginosa PA7 Transition g.582729A>G p.Lys92Arg
 Missense
AAG(Lys) -> AGG(Arg) 		SNP-192236 View
Select to view SNPs in a specific strain
SNP Map
PubMed Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE.
Genome Res. 2005 Feb;15(2):321-9.
PMID: 15687295
Purification and characterization of nitrous oxide reductase from Pseudomonas aeruginosa strain P2.
SooHoo CK, Hollocher TC.
J Biol Chem. 1991 Feb 5;266(4):2203-9.
PMID: 1899237
Extended x-ray absorption fine structure and electron paramagnetic resonance of nitrous oxide reductase from Pseudomonas aeruginosa strain P2.
SooHoo CK, Hollocher TC, Kolodziej AF, Orme-Johnson WH, Bunker G.
J Biol Chem. 1991 Feb 5;266(4):2210-8.
PMID: 1846618
The structural genes for nitric oxide reductase from Pseudomonas aeruginosa.
Arai H, Igarashi Y, Kodama T.
Biochim Biophys Acta. 1995 Apr 4;1261(2):279-84.
PMID: 7711073
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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