Pseudomonas Genome Database - Annotation for PA0552

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PA0552

Key to diagram

View in GBrowse:

16 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Operons/Regulons
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name	pgk	Alternate Gene Names
Locus tag	PA0552 (View 6 updates) Submit an Update	GenBank Identifier (GI)	15595749 (Precomputed BLAST results at BLINK )
Genomic location	611281 - 612444 (+)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG000536
Product Information
Product Name	phosphoglycerate kinase (Class 2 )
Alternate Product Names
Subcellular localization	Cytoplasmic (Class 2) Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA00553
Systomonas	PA0552
RefSeq	NP_249243.1
GI	15595749
KEGG	PA0552
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Operons/Regulons

Type

operon

Genes

Gene	Product
epd	D-erythrose 4-phosphate dehydrogenase
pgk	phosphoglycerate kinase
PA0553	hypothetical protein

Evidence

Computationally Predicted

PubMed

18988623

Cross-Reference

DOOR-13223

Genomic Context

Genomic context

- Adjacent to epd ORF, as in E. coli

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Carbon compound catabolism
Energy metabolism

GO Accession

GO Term

Evidence

Molecular Function

GO:0004618

phosphoglycerate kinase activity

RCA

Biological Process

GO:0044248	cellular catabolic process	ISS
GO:0015978	carbon utilization by utilization of organic compounds	ISS
GO:0006091	generation of precursor metabolites and energy	ISS
GO:0006096	glycolysis	RCA

Cellular Component

COG Prediction

Accession	Description	Category
COG0126	Pgk, 3-phosphoglycerate kinase [Carbohydrate transport and metabolism].	Carbohydrate transport and metabolism

Pathways

Name	Database	Xref
Glycolysis / Gluconeogenesis	PseudoCAP
Carbon fixation	PseudoCAP
gluconeogenesis	PseudoCyc	GLUCONEO-PWY
aerobic glycerol degradation I	PseudoCyc	AERO-GLYCEROL-FERM-PWY
2-dehydro-D-gluconate pyruvate degradation	PseudoCyc	DHGLUCONATE-PYR-CAT-PWY
sorbitol metabolism	PseudoCyc	P461-PWY
glycolysis+Entner-Doudoroff	PseudoCyc	GLYCOLYSIS-E-D
heterofermentative lactate fermentation	PseudoCyc	P122-PWY
anaerobic glycolysis	PseudoCyc	ANAGLYCOLYSIS-PWY
glycolysis	PseudoCyc	GLYCOLYSIS
glycolysis+TCA+glyoxylate bypass	PseudoCyc	GLYCOLYSIS-TCA-GLYOX-BYPASS
glyceraldehyde 3-phosphate degradation	PseudoCyc	P81-PWY
	PseudoCyc	GLYCOLYSIS+CITRIC-ACID-PWY
Glycolysis / Gluconeogenesis	KEGG	pae00010	Computationally Generated
Metabolic pathways	KEGG	pae01100	Computationally Generated

Reactions

EC Number

2.7.2.3

Enzyme Assays

PDF file(s) provided by Sigma-Aldrich:
3-Phosphoglyceric Phosphokinase (EC 2.7.2.3) (2.7.2.3)

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

82% similar to pgk gene product of E. coli

Most Similar Human Homolog

ENSP00000362413, Percent Identity: 39.42%, E-Value: 4e-60, Ensembl Release: 56.
Description: Phosphoglycerate kinase 1

Structure Features

PF00162: Phosphoglycerate kinases

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF00162	PGK, Phosphoglycerate kinase.	8	381	1e-111	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

ATGAACGACCAGCCGGTGATCGACGCCTACCACCATGAGGACCTGCGCCGTACCCGTTCGGCGTTCCAGTCGGTGATCCCGGTGTCCACCGGTCTGGCGC GCGGCATCGAACGCCTGTTGCCGGAACTTGCCGGGCGCATCCAGGCCAAAGCCATACGCGTGCCCACGGTGAACGTCTCGGCGCTGGACATCACCCTGCA GACTTCCCGCGACACCTCCGCCGCCGAGATCAACCGGGTGCTTCGCGAGGCTGCCGAAAGCGGCCCGCTGCGCGGACTGCTGGCTTATACCGAGCTGCCG CACGCCAGTTGCGATTTCAACCACGACCCCCATTCGGCCATCGTCGATGGCAGCCAGACCCGCGTTTCCGGGCCTCGCCTGGTCAACTTGCTGGCCTGGT TCGACAACGAATGGGGATTCGCCAACCGTATGCTCGACGTCGCCGGACACTACCTGCGCGCCGCCGTGCCTGATCAACAAGTACCCAAGGACTGATTGTC

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGACCGTGTTGAAGATGACTGACCTCGACCTCAAAGGTAAGCGCGTGCTGATCCGCGAGGACCTCAACGTGCCCGTGAAGGACGGCCAGGTGCAGAGCG ATGCACGGATCAAGGCCGCCCTGCCGACCCTCAAGCTGGCCCTGGAGAAGGGCGCGGCGGTGATGGTCTGCTCGCACCTGGGCCGTCCGACCGAGGGCGA GTTCTCCGCCGAGAACAGCCTGAAGCCGGTGGCCGAGTACCTGTCCAAGGCCCTGGGCCGCGAAGTGCCGCTGCTGGCCGACTACCTCGACGGCGTCGAG GTCAAGGCGGGCGACCTGGTGCTGTTCGAGAACGTGCGCTTCAACAAGGGCGAGAAGAAGAACGCCGACGAGCTGGCGCAGAAGTACGCCGCGCTGTGCG ACGTGTTCGTGATGGATGCCTTCGGTACCGCCCACCGCGCCGAGGGGTCGACCCACGGCGTGGCCAGGTTCGCCAAGGTCGCCGCCGCCGGCCCGCTGCT GGCCGCCGAACTGGACGCGCTGGGCAAGGCGCTGGGCAACCCGGCGCGGCCGATGGCGGCGATCGTCGCCGGCTCCAAGGTTTCCACCAAGCTCGACGTG CTGAACAGCCTGGCCGGCATCTGCGACCAACTGATCGTCGGCGGCGGCATCGCCAACACCTTCCTCGCCGCGGCCGGACACAAGGTCGGCAAGTCACTGT ACGAGGCCGACCTGGTCGAGACCGCCAAGGCCATCGCCGCCAAGGTCAAGGTGCCGCTGCCGGTGGACGTGGTGGTGGCCAAGGAATTCGCCGAGAGCGC CGTGGCGACCGTCAAGGCCATCGCCGAGGTCGCCGACGACGACATGATCCTCGACATCGGCCCGCAGACCGCCGCGCAGTTCGCCGAGCTGCTGAAGACC TCGAAGACCATCCTGTGGAACGGTCCGGTCGGGGTGTTCGAGTTCGACCAGTTCGGCGAGGGCACCCGTACCCTGGCCAATGCCATCGCCGATAGCGCGG CGTTCTCCATCGCCGGCGGCGGCGATACCCTGGCGGCGATCGACAAGTACGGCATCGCCGAGCGGATCTCCTATATTTCCACCGGTGGCGGCGCGTTCCT CGAGTTCGTCGAGGGCAAGGTCCTGCCGGCCGTGGAAATCCTGGAGCAGCGCGCCAAGGGCTGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MTVLKMTDLDLKGKRVLIREDLNVPVKDGQVQSDARIKAALPTLKLALEKGAAVMVCSHLGRPTEGEFSAENSLKPVAEYLSKALGREVPLLADYLDGVE VKAGDLVLFENVRFNKGEKKNADELAQKYAALCDVFVMDAFGTAHRAEGSTHGVARFAKVAAAGPLLAAELDALGKALGNPARPMAAIVAGSKVSTKLDV LNSLAGICDQLIVGGGIANTFLAAAGHKVGKSLYEADLVETAKAIAAKVKVPLPVDVVVAKEFAESAVATVKAIAEVADDDMILDIGPQTAAQFAELLKT SKTILWNGPVGVFEFDQFGEGTRTLANAIADSAAFSIAGGGDTLAAIDKYGIAERISYISTGGGAFLEFVEGKVLPAVEILEQRAKG

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0552 gene of Pseudomonas aeruginosa PAO1, Chromosome (611281 to 612444 bp)

Displaying the first five SNPs out of a total of 43 found in all isolate strains ( View all 43 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa PA7	Transversion	g.611352G>T		Silent GTG(Val) -> GTT(Val)	SNP-193728	View
Pseudomonas aeruginosa PA7	Transversion	g.611355C>G		Silent CCC(Pro) -> CCG(Pro)	SNP-193729	View
Pseudomonas aeruginosa PA7	Transversion	g.611410C>A	p.Leu44Ile	Missense CTC(Leu) -> ATC(Ile)	SNP-193730	View
Pseudomonas aeruginosa PA7	Transition	g.611414A>G	p.Lys45Arg	Missense AAG(Lys) -> AGG(Arg)	SNP-193731	View
Pseudomonas aeruginosa 2192	Transversion	g.611466T>A		Silent CGT(Arg) -> CGA(Arg)	SNP-69669	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Cloning and characterization of the Escherichia coli phosphoglycerate kinase (pgk) gene.
Nellemann LJ, Holm F, Atlung T, Hansen FG.
Gene. 1989 Apr 15;77(1):185-91.
PMID: 2545537
Identification, molecular cloning and sequence analysis of a gene cluster encoding the class II fructose 1,6-bisphosphate aldolase, 3-phosphoglycerate kinase and a putative second glyceraldehyde 3-phosphate dehydrogenase of Escherichia coli.
Alefounder PR, Perham RN.
Mol Microbiol. 1989 Jun;3(6):723-32.
PMID: 2546007
Crystallographic analysis of phosphoglycerate kinase from the hyperthermophilic bacterium Thermotoga maritima.
Auerbach G, Jacob U, Grattinger M, Schurig H, Jaenicke R.
Biol Chem. 1997 Mar-Apr;378(3-4):327-9.
PMID: 9165089