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               PA0555               
Key to diagram


View in GBrowse: 16 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name fda  Alternate Gene Names cfxB, cbbA, fbaA
Locus tag PA0555 (View 8 updates) Submit an Update GenBank Identifier (GI) 15595752 (Precomputed BLAST results at BLINK )
Genomic location 613338 - 614402 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help No transposon mutant available yet Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name fructose-1,6-bisphosphate aldolase (Class 2  Explanation of Product Name Confidence)
Alternate Product Names Fructose-1,6-bisphosphate triosephosphate-lyase
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA00556
Systomonas PA0555
RefSeq NP_249246.1
GI 15595752
KEGG PA0555
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
fda fructose-1,6-bisphosphate aldolase
PA0556 hypothetical protein
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13224
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Central intermediary metabolism
Carbon compound catabolism
GO Accession GO Term Evidence
Molecular Function
GO:0004332 fructose-bisphosphate aldolase activity RCA
GO:0004332 fructose-bisphosphate aldolase activity IEA
Biological Process
GO:0044248 cellular catabolic process ISS
GO:0015978 carbon utilization by utilization of organic compounds ISS
GO:0008152 metabolic process ISS
GO:0006096 glycolysis RCA
GO:0006096 glycolysis IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0191 Fba, Fructose/tagatose bisphosphate aldolase [Carbohydrate transport and metabolism]. Carbohydrate transport and metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Pentose phosphate cycle PseudoCAP
Glycolysis / Gluconeogenesis PseudoCAP
Fructose and mannose metabolism PseudoCAP
Carbon fixation PseudoCAP
gluconeogenesis PseudoCyc GLUCONEO-PWY
sorbitol metabolism PseudoCyc P461-PWY
glycolysis+Entner-Doudoroff PseudoCyc GLYCOLYSIS-E-D
anaerobic glycolysis PseudoCyc ANAGLYCOLYSIS-PWY
glycolysis PseudoCyc GLYCOLYSIS
glycolysis+TCA+glyoxylate bypass PseudoCyc GLYCOLYSIS-TCA-GLYOX-BYPASS
PseudoCyc GLYCOLYSIS+CITRIC-ACID-PWY
Glycolysis / Gluconeogenesis KEGG pae00010 Computationally Generated
Pentose phosphate pathway KEGG pae00030 Computationally Generated
Fructose and mannose metabolism KEGG pae00051 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 4.1.2.13
Enzyme Assays
PDF file(s) provided by Sigma-Aldrich:
 Aldolase (4.1.2.13)
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 97% similar to fda gene product of [Pseudomonas stutzeri]
82% similar to cbbA gene products [Alcaligenes eutrophus], [Xanthobacter flavus]
Structure Features
Structure Features Explanation of Structure Features Field PS00602: Fructose-bisphosphate aldolase class-II signature 1
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF01116 F_bP_aldolase, Fructose-bisphosphate aldolase class-II. 2 327 1e-104 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region GAGGTGCGGCGGAGCGTTCCGCACGGATGGAGGATGGACATGAACTACTCGCGTATCGCCCTGTTCGGCGGCTTCGCCGCCGCGGCGCTGCTGGCCGGCT GCTCCGCTTCCTCCGGCAAGCCGGGCGGCAAGGACTGGACCCGCTGGACCTGCGACAGCAAGGCGGTGATCGAATGGCGCTATATCGACGGCAGCAAGAA CATCGTCGACCTGCGCCTGAAGGACGAGGACGACGTGGTCCACCACTTGGAGCAGGAACCTGCGGCCGTCGGCGCTTTCTATAGCGACGGACGCCTGGGC TTCCACCTGGAGAACGACGAAGGCCTGGTGTACTGGGTCGAAACCGACGACCTCATCGGTCGCGGTTGCAAGGCGCGCTGAGTCGCGCCGGCAGGGGCGC GGCGCCGCGACCGCTCCTCGCAAGGAACTTGAACCGGGCCTGCCCCTGCGGCAGGCTTCTGAATCACTGAACGACCCCGCGATTGGGAGATACGAGAACC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGGCACTCATCAGCATGCGCCAGATGCTGGATCACGCCGCCGAATTCGGCTACGGCGTGCCGGCCTTCAACGTCAACAACCTCGAGCAGATGCGCGCCA TCATGGAAGCGGCGGACAAGACCGATTCGCCCGTGATCGTCCAGGCTTCCGCCGGTGCTCGCAAATACGCCGGCGCGCCCTTCCTGCGCCACCTGATCCT CGCCGCGATCGAAGAATTCCCGCACATCCCGGTGGTCATGCACCAGGATCACGGCACCAGTCCCGACGTCTGCCAGCGTTCCATCCAGCTGGGCTTCAGC TCGGTGATGATGGACGGCTCCCTGCGTGAAGACGGCAAGACCCCTGCCGACTACGACTACAACGTCCGTGTGACCCAGCAGACCGTGGCGTTCGCCCATG CCTGCGGCGTGTCCGTGGAAGGCGAGCTGGGCTGCCTGGGTTCCCTGGAAACCGGCATGGCCGGCGAGGAAGACGGCGTCGGCGCGGAAGGCGTGCTGGA CCACAGCCAGTTGCTGACCGATCCGGAAGAAGCCGCCGACTTCGTGAAGAAGACCAAGGTCGACGCCCTGGCCATCGCCATCGGCACCAGCCACGGCGCC TACAAGTTCACCAAGCCGCCGACCGGGGACACCCTGTCGATCCAGCGCATCAAGGAAATCCACGCGCGCATCCCGGACACCCACCTGGTGATGCACGGCT CTTCCTCGGTACCGCAGGACTGGCTGGCGATCATCAACGAGTACGGCGGCGAGATCAAAGAGACCTACGGCGTGCCGGTCGAGGAAATCGTCGAGGGCAT CAAGTACGGCGTGCGCAAGGTGAACATCGACACCGACCTGCGCCTGGCCTCCACCGGTGCCATCCGCCGCTTCCTGGCGCAGAACCCGAGCGAGTTCGAC CCGCGCAAGTACTTCTCCAAGACCGTCGAGGCCATGCGCGACATCTGCATCGCTCGCTACGAAGCCTTCGGCACCGCCGGCAACGCTTCCAAGATCAAGC CGATCTCCCTGGAAGGCATGTTCCAGCGCTACGCTCGCGGCGAGCTGGATCCGAAGGTCAACTAA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MALISMRQMLDHAAEFGYGVPAFNVNNLEQMRAIMEAADKTDSPVIVQASAGARKYAGAPFLRHLILAAIEEFPHIPVVMHQDHGTSPDVCQRSIQLGFS SVMMDGSLREDGKTPADYDYNVRVTQQTVAFAHACGVSVEGELGCLGSLETGMAGEEDGVGAEGVLDHSQLLTDPEEAADFVKKTKVDALAIAIGTSHGA YKFTKPPTGDTLSIQRIKEIHARIPDTHLVMHGSSSVPQDWLAIINEYGGEIKETYGVPVEEIVEGIKYGVRKVNIDTDLRLASTGAIRRFLAQNPSEFD PRKYFSKTVEAMRDICIARYEAFGTAGNASKIKPISLEGMFQRYARGELDPKVN

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0555 gene of Pseudomonas aeruginosa PAO1, Chromosome (613338 to 614402 bp)


Displaying the first five SNPs out of a total of 33 found in all isolate strains ( View all 33 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa C3719 Transversion g.613451G>C
Silent
GCG(Ala) -> GCC(Ala) 		SNP-38090 View
Pseudomonas aeruginosa 2192 Transversion g.613451G>C
Silent
GCG(Ala) -> GCC(Ala) 		SNP-69674 View
Pseudomonas aeruginosa LESB58 Transversion g.613451G>C
Silent
GCG(Ala) -> GCC(Ala) 		SNP-73590 View
Pseudomonas aeruginosa PACS2 Transversion g.613451G>C
Silent
GCG(Ala) -> GCC(Ala) 		SNP-113372 View
Pseudomonas aeruginosa PA14 Transversion g.613451G>C
Silent
GCG(Ala) -> GCC(Ala) 		SNP-130614 View
Select to view SNPs in a specific strain
SNP Map
PubMed Cloning, sequence analysis and over-expression of the gene for the class II fructose 1,6-bisphosphate aldolase of Escherichia coli.
Alefounder PR, Baldwin SA, Perham RN, Short NJ.
Biochem J. 1989 Jan 15;257(2):529-34.
PMID: 2649077
Analysis of the genes forming the distal parts of the two cbb CO2 fixation operons from Alcaligenes eutrophus.
Schaferjohann J, Yoo JG, Bowien B.
Arch Microbiol. 1995 Apr;163(4):291-9.
PMID: 7763137
Primary structure and phylogeny of the Calvin cycle enzymes transketolase and fructosebisphosphate aldolase of Xanthobacter flavus.
van den Bergh ER, Baker SC, Raggers RJ, Terpstra P, Woudstra EC, Dijkhuizen L, Meijer WG.
J Bacteriol. 1996 Feb;178(3):888-93.
PMID: 8550527
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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