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               PA0649               
Key to diagram


View in GBrowse: 16 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name trpG  Alternate Gene Names pabA
Locus tag PA0649 (View 8 updates) Submit an Update GenBank Identifier (GI) 15595846 (Precomputed BLAST results at BLINK )
Genomic location 703477 - 704082 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name anthranilate synthase component II (Class 1  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Cytoplasmic (Class 1Explanation of Localization Confidence)
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA00650
Systomonas PA0649
RefSeq NP_249340.1
GI 15595846
KEGG PA0649
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
trpG anthranilate synthase component II
trpD anthranilate phosphoribosyltransferase
trpC indole-3-glycerol-phosphate synthase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13249
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Energy metabolism
Biosynthesis of cofactors, prosthetic groups and carriers
Amino acid biosynthesis and metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0004049 anthranilate synthase activity RCA
GO:0016884 carbon-nitrogen ligase activity, with glutamine as amido-N-donor IEA
Biological Process
GO:0006520 amino acid metabolic process IDA
GO:0051188 cofactor biosynthetic process IDA
GO:0006091 generation of precursor metabolites and energy IDA
GO:0000162 tryptophan biosynthetic process RCA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0512 PabA, Anthranilate/para-aminobenzoate synthases component II [Amino acid transport and metabolism / Coenzyme metabolism]. Amino acid transport and metabolism / Coenzyme metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Ubiquinone biosynthesis PseudoCAP
Phenylalanine, tyrosine and tryptophan biosynthesis PseudoCAP
Folate biosynthesis PseudoCAP
phenylalanine, tyrosine and tryptophan biosynthesis, complete PseudoCyc COMPLETE-ARO-PWY
tryptophan biosynthesis PseudoCyc TRPSYN-PWY
pathways of chorismate PseudoCyc ALL-CHORISMATE-PWY
Phenylalanine, tyrosine and tryptophan biosynthesis KEGG pae00400 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 4.1.3.27
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 83% similar to p-aminobenzoate synthetase component II PabA [Escherichia coli]
99% similar to anthranilate synthase component II TrpG [Pseudomonas aeruginosa]
Most Similar Human Homolog
ENSP00000384510, Percent Identity: 27.68%, E-Value: 4e-08, Ensembl Release: 56.
Description: CAD protein
Structure Features
Structure Features Explanation of Structure Features Field PS00442: Glutamine amidotransferases class-I active site
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00117 GATase, Glutamine amidotransferase class-I. 3 192 2e-61 23.0
PF07722 Peptidase_C26, Peptidase C26. 62 175 3e-05 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region TGCAGGTGTGGAGACGGATTACAGCGCCTGGCTTGCCAAGGTCGAAGAGATCAAGGCTAGATATCCGCTCCCGCGCATCTGACCTGGCTGATGGACGCAA GCCTGCCTCGTGTAGGCTTCGCCATTTTAGATCGCTACTGTCGGGCCTCTGCCTGGTAAACAATTTCCGCTTCTTAAGCCTCTTTCTTCCGACATCCCGC TGTTGCACCCATCCTCCCCACTGGGCTATGTTCCCCAACATGGCTCCGTGAGACCGCTATCGTGATCCACCTCAGCAGCATCATCACCTCCCTCATCAAG GCATTCGCCCGTTGGCGCTGGCGCGCCTGACTTCTTCTTCGCCGGCCTGGCCGGTCACGTCTCCCTGCGTACCCCGTTTTCCACAGGCATAATGCCGACG ATCCACTCCCCCTTCCTCCGCGAGCGCGCCGGGAAACGGGTGTGGCGAAAGCCGGGTGATGCGATTCGCATAGGAATGAAATCAAGAGGTTACAGCCAGC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGCTGCTGATGATCGATAACTACGACTCCTTCACCTACAACCTGGTGCAGTACTTCGGCGAGCTCAAGGCCGAGGTCAAGGTGGTGCGTAACGACGAAC TGAGCGTGGAACAGATCGAGGCCCTGGCGCCCGAGCGCATCGTCCTTTCCCCCGGTCCCTGCACCCCCAACGAGGCCGGCGTCTCGCTGGCCGTTATCGA GCGCTTCGCCGGCAAGCTGCCGCTGCTCGGTGTCTGCCTTGGCCATCAGAGCATCGGCCAGGCCTTCGGCGGCGAAGTGGTGCGGGCGCGGCAGGTGATG CACGGCAAGACCAGCCCGATCCACCACAAGGACCTCGGCGTGTTCGCCGGCCTGGCCAATCCCCTGACGGTGACGCGCTACCACTCGCTGGTGGTGAAGC GAGAGAGCCTGCCGGAGTGCCTGGAGGTCACCGCCTGGACCCAACATGCCGACGGATCGCTCGACGAGATCATGGGCGTACGCCACAAGACCCTGAATGT CGAGGGCGTGCAGTTCCATCCCGAGTCCATCCTCACCGAGCAGGGCCACGAGTTGCTGGCCAACTTCCTCCGCCAGCAGGGCGGCGTGCGTGGGGAGGGT AACTGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MLLMIDNYDSFTYNLVQYFGELKAEVKVVRNDELSVEQIEALAPERIVLSPGPCTPNEAGVSLAVIERFAGKLPLLGVCLGHQSIGQAFGGEVVRARQVM HGKTSPIHHKDLGVFAGLANPLTVTRYHSLVVKRESLPECLEVTAWTQHADGSLDEIMGVRHKTLNVEGVQFHPESILTEQGHELLANFLRQQGGVRGEG N

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0649 gene of Pseudomonas aeruginosa PAO1, Chromosome (703477 to 704082 bp)


Displaying the first five SNPs out of a total of 68 found in all isolate strains ( View all 68 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PACS2 Transition g.703521G>A
Silent
CTG(Leu) -> CTA(Leu) 		SNP-113604 View
Pseudomonas aeruginosa C3719 Transition g.703539G>A
Silent
GAG(Glu) -> GAA(Glu) 		SNP-16915 View
Pseudomonas aeruginosa LESB58 Transition g.703539G>A
Silent
GAG(Glu) -> GAA(Glu) 		SNP-70992 View
Pseudomonas aeruginosa PA14 Transition g.703539G>A
Silent
GAG(Glu) -> GAA(Glu) 		SNP-145093 View
Pseudomonas aeruginosa PA7 Transition g.703539G>A
Silent
GAG(Glu) -> GAA(Glu) 		SNP-442139 View
Select to view SNPs in a specific strain
SNP Map
PubMed DNA sequences and characterization of four early genes of the tryptophan pathway in Pseudomonas aeruginosa.
Essar DW, Eberly L, Han CY, Crawford IP.
J Bacteriol. 1990 Feb;172(2):853-66.
PMID: 2105306
Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications.
Essar DW, Eberly L, Hadero A, Crawford IP.
J Bacteriol. 1990 Feb;172(2):884-900.
PMID: 2153661
PSORTb 3.0: Improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes.
Yu NY, Wagner JR, Laird MR, Melli G, Rey S, Lo R, Dao P, Sahinalp SC, Ester M, Foster LJ, Brinkman FS.
Bioinformatics. 2010 May 13; [Epub ahead of print]
PMID: 20472543
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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