Pseudomonas Genome Database - Annotation for PA0743

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PA0743

Key to diagram

View in GBrowse:

15 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name		Alternate Gene Names
Locus tag	PA0743 (View 6 updates) Submit an Update	GenBank Identifier (GI)	15595940 (Precomputed BLAST results at BLINK )
Genomic location	811171 - 810275 (-)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG000717
Product Information
Product Name	probable 3-hydroxyisobutyrate dehydrogenase (Class 3 )
Alternate Product Names
Subcellular localization	Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA00744
Systomonas	PA0743
RefSeq	NP_249434.1
GI	15595940
KEGG	PA0743
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Carbon compound catabolism

GO Accession

GO Term

Evidence

Molecular Function

GO:0008442

3-hydroxyisobutyrate dehydrogenase activity

IEA

Biological Process

GO:0006574

valine catabolic process

IEA

Cellular Component

COG Prediction

Accession	Description	Category
COG2084	MmsB, 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases [Lipid metabolism].	Lipid metabolism

Pathways

Name	Database	Xref
valine degradation I	PseudoCyc	VALDEG-PWY
Valine, leucine and isoleucine degradation	KEGG	pae00280	Computationally Generated
Metabolic pathways	KEGG	pae01100	Computationally Generated

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

75% similarity to MmsB (3-hydroxyisobutyrate dehydrogenase)[Pseudomonas aeruginosa]

Most Similar Human Homolog

ENSP00000265395, Percent Identity: 44.91%, E-Value: 1e-52, Ensembl Release: 56.
Description: 3-hydroxyisobutyrate dehydrogenase, mitochondrial Precursor

Structure Features

PS00895:3-hydroxyisobutyrate dehydrogenase signature

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF03446	NAD_binding_2, NAD binding domain of 6-phosphogluconate dehydrogenase.	1	163	6e-59	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

GCCGGCCGCGCCTGGACGCACTGCTCGACCAGCCGGACCTGGCGAGCGCCTGGCAGGCGCTGGTGGCGCTGCGCGACGACGCCGACCCGCTGCTTGCGCG TGGCGCGAAGACCCTGGCCGAGGGCTGCCCGATGACCGCGCATCTGGTCTGGCAGCAGATCGAGCGTGCGCGCTACCTGTCGCTGGCCGAGGTGTTCCGC CTGGAATACGCCATGAGCCTGAACTGCACGCGCCATCCGGACTTCGCCGAGGGGGTACGCGCAAGGCTGATCGATCGCGACAACGCACCGAACTGGCATT GGCCGCAGGTGGAATCGATCCCGCAGGCGGTGATCGAGGCGCACTTCGAGCCGACCTGGGAGGGCGAGCATCCGCTGGCGGGGCTCTGATCCGCCGCGCC GCCCTCTGCCGAAGGAGGGCGCGGGCCCGCCACGTCCATAGCGTCTAAGCTGTTTCACAGCATCCCCAAGAGGCCTCGCGGCCACCATAGGAGGAGCTCC

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGAAACAGATCGCCTTCATCGGCCTCGGCCACATGGGCGCGCCGATGGCCACCAACCTGCTCAAGGCCGGCTACCTGCTGAACGTCTTCGACCTGGTGC AGAGCGCCGTCGACGGCCTGGTCGCCGCCGGCGCCAGCGCCGCCCGCAGCGCCCGCGACGCGGTGCAGGGCGCCGACGTGGTCATCAGCATGCTGCCGGC CAGCCAGCACGTCGAAGGCCTGTACCTCGACGACGATGGCCTGCTCGCGCACATCGCCCCCGGCACCCTGGTGCTGGAATGCTCGACCATCGCCCCGACC TCGGCGCGCAAGATCCACGCCGCCGCCCGCGAGCGCGGCCTGGCGATGCTCGACGCGCCGGTGTCCGGCGGCACTGCCGGCGCGGCAGCCGGCACCCTGA CCTTCATGGTCGGCGGCGACGCCGAAGCCCTGGAGAAGGCCCGGCCGCTGTTCGAGGCCATGGGTCGCAACATCTTCCATGCCGGCCCCGACGGCGCCGG CCAGGTCGCCAAGGTGTGCAACAACCAACTCCTCGCGGTGCTGATGATCGGTACCGCAGAAGCCATGGCCCTGGGCGTGGCCAACGGCCTCGAGGCGAAG GTGCTGGCCGAGATCATGCGCCGCAGCTCCGGCGGCAACTGGGCGCTGGAAGTCTACAACCCCTGGCCGGGGGTGATGGAGAACGCTCCGGCGTCGCGCG ACTACAGCGGCGGCTTCATGGCCCAGTTGATGGCCAAGGACCTCGGCCTGGCCCAGGAAGCCGCCCAGGCCAGCGCCAGCAGCACGCCGATGGGCAGCCT GGCGCTGAGCCTGTACCGCCTGTTGCTGAAGCAGGGCTATGCGGAGCGGGACTTTTCCGTGGTGCAGAAATTGTTCGATCCGACACAAGGCCAATAG

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MKQIAFIGLGHMGAPMATNLLKAGYLLNVFDLVQSAVDGLVAAGASAARSARDAVQGADVVISMLPASQHVEGLYLDDDGLLAHIAPGTLVLECSTIAPT SARKIHAAARERGLAMLDAPVSGGTAGAAAGTLTFMVGGDAEALEKARPLFEAMGRNIFHAGPDGAGQVAKVCNNQLLAVLMIGTAEAMALGVANGLEAK VLAEIMRRSSGGNWALEVYNPWPGVMENAPASRDYSGGFMAQLMAKDLGLAQEAAQASASSTPMGSLALSLYRLLLKQGYAERDFSVVQKLFDPTQGQ

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0743 gene of Pseudomonas aeruginosa PAO1, Chromosome (810275 to 811171 bp)

Displaying the first five SNPs out of a total of 86 found in all isolate strains ( View all 86 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa C3719	Transversion	g.810287T>G		Silent ACA(Thr) -> ACC(Thr)	SNP-21863	View
Pseudomonas aeruginosa 2192	Transversion	g.810287T>G		Silent ACA(Thr) -> ACC(Thr)	SNP-66029	View
Pseudomonas aeruginosa LESB58	Transversion	g.810287T>G		Silent ACA(Thr) -> ACC(Thr)	SNP-92538	View
Pseudomonas aeruginosa PACS2	Transversion	g.810287T>G		Silent ACA(Thr) -> ACC(Thr)	SNP-100680	View
Pseudomonas aeruginosa PA14	Transversion	g.810287T>G		Silent ACA(Thr) -> ACC(Thr)	SNP-160685	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Characterization of the mmsAB operon of Pseudomonas aeruginosa PAO encoding methylmalonate-semialdehyde dehydrogenase and 3-hydroxyisobutyrate dehydrogenase.
Steele MI, Lorenz D, Hatter K, Park A, Sokatch JR.
J Biol Chem. 1992 Jul 5;267(19):13585-92.
PMID: 1339433