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               PA0744               
Key to diagram


View in GBrowse: 16 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name   Alternate Gene Names
Locus tag PA0744 (View 4 updates) Submit an Update GenBank Identifier (GI) 15595941 (Precomputed BLAST results at BLINK )
Genomic location 812386 - 811283 (-) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name probable enoyl-CoA hydratase/isomerase (Class 3  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA00745
Systomonas PA0744
RefSeq NP_249435.1
GI 15595941
KEGG PA0744
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
PA0744 probable enoyl-CoA hydratase/isomerase
PA0745 probable enoyl-CoA hydratase/isomerase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13269
Genomic Context
Genomic context Explanation of Genomic Context Field - In cluster of genes that appears similar to pathways involved in degradation of carbon compounds, including genes for beta-oxidation.
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Putative enzymes
GO Accession GO Term Evidence
Molecular Function
GO:0004300 enoyl-CoA hydratase activity IEA
Biological Process
GO:0010124 phenylacetate catabolic process IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG1024 CaiD, Enoyl-CoA hydratase/carnithine racemase [Lipid metabolism]. Lipid metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Fatty acid metabolism KEGG pae00071 Computationally Generated
Valine, leucine and isoleucine degradation KEGG pae00280 Computationally Generated
Geraniol degradation KEGG pae00281 Computationally Generated
Lysine degradation KEGG pae00310 Computationally Generated
Tryptophan metabolism KEGG pae00380 Computationally Generated
beta-Alanine metabolism KEGG pae00410 Computationally Generated
Benzoate degradation via CoA ligation KEGG pae00632 Computationally Generated
Propanoate metabolism KEGG pae00640 Computationally Generated
Butanoate metabolism KEGG pae00650 Computationally Generated
Limonene and pinene degradation KEGG pae00903 Computationally Generated
Caprolactam degradation KEGG pae00930 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 52% similarity to putative enoyl-coenzyme A hydratase [Pseudomonas putida]
Most Similar Human Homolog
ENSP00000387247, Percent Identity: 39.29%, E-Value: 2e-18, Ensembl Release: 56.
Description: 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial Precursor
Structure Features
Structure Features Explanation of Structure Features Field PS00166: Enoyl-CoA hydratase/isomerase signature
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00378 ECH, Enoyl-CoA hydratase/isomerase family. 20 191 3e-18 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region GTGCGCCCTCGCCTGCGACATCCGCATCGCCGAGCGCCAGGCGCAGATGGCCCTGCCGGAGGCCGCGGTGGGCCTGCTGCCCTGCGCCGGCGGGACCCAG GCGCTGCCCTGGCTGGTGGGCGAAGGCTGGGCCAAGCGGATGATCCTCTGCAACGAGCGGGTGGATGCGGAAACCGCCCTGCGCATCGGCCTGGTCGAAC AGGTGGTGGACAGCGGCGAGGCGCGCGGCGCCGCCCTGCTGCTGGCGGCCAAGGTGGCACGCCAGAGCCCGGTGGCGATCCGCACCATCAAGCCGCTGAT CCAGGGTGCCCGCGAACGCGCGCCGAACACTTGGCTGCCGGAGGAGCGCGAGCGCTTCGTCGATCTGTTCGACGCCCAGGACACCCGCGAAGGGGTCAAC GCCTTCCTCGAGAAGCGCGATCCCAAGTGGCGCAACTGCTGAGCCTTCCCATCGAACCCGGCGGGCTGCGCCACAGCCCGCCCGAGCCGAGAGCCCCGAC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGAACGTGCTTTTCGAAGAACGCCCGAGCCTGCATGGCTTCCGCATCGGCATTGCCACCCTGGATGCGGAAAAGAGCCTGAACGCCCTGTCCCTGCCGA TGATCGAGGCCCTGGCGGCGAAACTCGACGCCTGGGCCGAGGACGCCGGCATCGCCTGCGTGCTGTTGCGCGGCAACGGCGCCAAGGCCTTTTGCGCCGG CGGCGACGTGCGCAAGCTGGTGGACGCCTGCCGTGAGCAACCCGGCGAGGTACCGGCCCTGGCGCGACGCTTCTTCGCCGATGAATACCGCCTCGACTAC CGCATCCACACCTACCCGAAACCCTTCATCTGCTGGGCCCACGGCTATGTGATGGGCGGCGGCATGGGGCTGATGCAGGGTGCCGGGATTCGCATCGTCA CCCCGTCCAGCCGCCTGGCGATGCCGGAGATCGGCATCGGCCTGTACCCAGACGTCGGCGCCAGCTGGTTCCTCGCCCGCCTGCCCGGCAGGCTCGGCCT GTTCCTCGGCCTGAGCGCGGCGCAGATGAACGCCCGCGACGCTCTCGACCTCGACCTGGCCGACCGCTTCCTTCTCGACGACCAGCAGGACGCCCTGCTC GCCGGCCTGGTGCAGATGAACTGGAACGAGTCGCCCCAGGTGCAACTTCACAGCCTGCTTCGCGCCCTCGAACACGAGGCCCGCGGCGAACTGCCGGAGG CCCAGTTGCTCCCGCGCCGGCCGCGCCTGGACGCACTGCTCGACCAGCCGGACCTGGCGAGCGCCTGGCAGGCGCTGGTGGCGCTGCGCGACGACGCCGA CCCGCTGCTTGCGCGTGGCGCGAAGACCCTGGCCGAGGGCTGCCCGATGACCGCGCATCTGGTCTGGCAGCAGATCGAGCGTGCGCGCTACCTGTCGCTG GCCGAGGTGTTCCGCCTGGAATACGCCATGAGCCTGAACTGCACGCGCCATCCGGACTTCGCCGAGGGGGTACGCGCAAGGCTGATCGATCGCGACAACG CACCGAACTGGCATTGGCCGCAGGTGGAATCGATCCCGCAGGCGGTGATCGAGGCGCACTTCGAGCCGACCTGGGAGGGCGAGCATCCGCTGGCGGGGCT CTGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MNVLFEERPSLHGFRIGIATLDAEKSLNALSLPMIEALAAKLDAWAEDAGIACVLLRGNGAKAFCAGGDVRKLVDACREQPGEVPALARRFFADEYRLDY RIHTYPKPFICWAHGYVMGGGMGLMQGAGIRIVTPSSRLAMPEIGIGLYPDVGASWFLARLPGRLGLFLGLSAAQMNARDALDLDLADRFLLDDQQDALL AGLVQMNWNESPQVQLHSLLRALEHEARGELPEAQLLPRRPRLDALLDQPDLASAWQALVALRDDADPLLARGAKTLAEGCPMTAHLVWQQIERARYLSL AEVFRLEYAMSLNCTRHPDFAEGVRARLIDRDNAPNWHWPQVESIPQAVIEAHFEPTWEGEHPLAGL

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0744 gene of Pseudomonas aeruginosa PAO1, Chromosome (811283 to 812386 bp)


Displaying the first five SNPs out of a total of 65 found in all isolate strains ( View all 65 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA7 Transition g.811290C>T p.Gly366Glu
 Missense
GGG(Gly) -> GAG(Glu) 		SNP-264446 View
Pseudomonas aeruginosa PA7 Transition g.811301A>G
Silent
CAT(His) -> CAC(His) 		SNP-264447 View
Pseudomonas aeruginosa PA7 Transition g.811304C>T
Silent
GAG(Glu) -> GAA(Glu) 		SNP-264448 View
Pseudomonas aeruginosa PA7 Transition g.811310C>T
Silent
GAG(Glu) -> GAA(Glu) 		SNP-264449 View
Pseudomonas aeruginosa PA14 Transition g.811345C>T p.Ala348Thr
 Missense
GCG(Ala) -> ACG(Thr) 		SNP-160699 View
Select to view SNPs in a specific strain
SNP Map
PubMed  


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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