Pseudomonas Genome Database - Annotation for PA0745

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PA0745

Key to diagram

View in GBrowse:

5 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Operons/Regulons
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name		Alternate Gene Names
Locus tag	PA0745 (View 4 updates) Submit an Update	GenBank Identifier (GI)	15595942 (Precomputed BLAST results at BLINK )
Genomic location	813263 - 812445 (-)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG000719
Product Information
Product Name	probable enoyl-CoA hydratase/isomerase (Class 3 )
Alternate Product Names
Subcellular localization	Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA00746
Systomonas	PA0745
RefSeq	NP_249436.1
GI	15595942
KEGG	PA0745
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Operons/Regulons

Type

operon

Genes

Gene	Product
PA0744	probable enoyl-CoA hydratase/isomerase
PA0745	probable enoyl-CoA hydratase/isomerase

Evidence

Computationally Predicted

PubMed

18988623

Cross-Reference

DOOR-13269

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Putative enzymes

GO Accession

GO Term

Evidence

Molecular Function

GO:0004300

enoyl-CoA hydratase activity

IEA

Biological Process

GO:0010124

phenylacetate catabolic process

IEA

Cellular Component

COG Prediction

Accession	Description	Category
COG1024	CaiD, Enoyl-CoA hydratase/carnithine racemase [Lipid metabolism].	Lipid metabolism

Pathways

Name	Database	Xref
Fatty acid metabolism	KEGG	pae00071	Computationally Generated
Valine, leucine and isoleucine degradation	KEGG	pae00280	Computationally Generated
Geraniol degradation	KEGG	pae00281	Computationally Generated
Lysine degradation	KEGG	pae00310	Computationally Generated
Tryptophan metabolism	KEGG	pae00380	Computationally Generated
beta-Alanine metabolism	KEGG	pae00410	Computationally Generated
Benzoate degradation via CoA ligation	KEGG	pae00632	Computationally Generated
Propanoate metabolism	KEGG	pae00640	Computationally Generated
Butanoate metabolism	KEGG	pae00650	Computationally Generated
Limonene and pinene degradation	KEGG	pae00903	Computationally Generated
Caprolactam degradation	KEGG	pae00930	Computationally Generated
Metabolic pathways	KEGG	pae01100	Computationally Generated

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

55% similar to crotonase [Clostridium acetobutylicum]

Most Similar Human Homolog

ENSP00000357535, Percent Identity: 33.60%, E-Value: 5e-28, Ensembl Release: 56.
Description: Enoyl-CoA hydratase, mitochondrial Precursor

Structure Features

PS00166: Enoyl-CoA hydratase/isomerase signature

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF00378	ECH, Enoyl-CoA hydratase/isomerase family.	28	195	4e-34	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

ATCGCCAGTTGTTCCCTGGGCGCCGCCCAGGCGGCGCTGGAGCAGTCGATGCGCTACGTCGAGGAGCGCGAGCAGTTCGGCAAGCCGCTGGCGACCTTCC AGGCCTTGCAGTTCAAGCTCGCCGACATGCTCACCGAACTCACCGCCAGCCGCCAGATGGTCCGCCTCGGCGCCCATCGGCTGGACCGCGGCGACGCCGA GGCGACCCTGTACTGCGCAATGGCCAAGCGCTTCGCCACCGACCGCTGCTTCGATGTCTGCAACGAGGCCTTGCAACTGCACGGCGGCTACGGCTATCTC AACGATTATCCGCTGGAGCGCTGGGTACGCGACACCCGCGTGCACCAGATCCTCGAAGGCACCAACGAAATCATGCGGGTGATCGTCGCCCGCCGCCTGC TGGAGCAGGGCGGCATGCTCGATCGCCTGCTGTGAACGCCGCGAACCATCGCATCCGATAGTGCCGGGACGCCCCGGCCCATGATCCAGTGAGGGACTTC

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGAACACTGCCGTCGAACCCTACAAGGCTTCCTCCTTCGACCTGACCCACAAGCTCACCGTGGAAAAGCACGGGCACACCGCGCTGATCACCATCAACC ACCCGCCGGCCAACACCTGGGACCGCGACTCGCTGATCGGCCTGCGCCAACTGATCGAGCACCTCAACCGCGACGACGATATCTACGCCCTGGTAGTGAC CGGCCAGGGGCCGAAGTTCTTCTCCGCCGGCGCCGACCTGAACATGTTCGCCGACGGCGACAAGGCCCGCGCTCGCGAGATGGCCCGCCGCTTCGGCGAA GCCTTCGAGGCGCTGCGCGATTTCCGCGGGGTGTCGATCGCGGCGATCAACGGCTACGCCATGGGCGGCGGCCTGGAGTGCGCCCTCGCCTGCGACATCC GCATCGCCGAGCGCCAGGCGCAGATGGCCCTGCCGGAGGCCGCGGTGGGCCTGCTGCCCTGCGCCGGCGGGACCCAGGCGCTGCCCTGGCTGGTGGGCGA AGGCTGGGCCAAGCGGATGATCCTCTGCAACGAGCGGGTGGATGCGGAAACCGCCCTGCGCATCGGCCTGGTCGAACAGGTGGTGGACAGCGGCGAGGCG CGCGGCGCCGCCCTGCTGCTGGCGGCCAAGGTGGCACGCCAGAGCCCGGTGGCGATCCGCACCATCAAGCCGCTGATCCAGGGTGCCCGCGAACGCGCGC CGAACACTTGGCTGCCGGAGGAGCGCGAGCGCTTCGTCGATCTGTTCGACGCCCAGGACACCCGCGAAGGGGTCAACGCCTTCCTCGAGAAGCGCGATCC CAAGTGGCGCAACTGCTGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MNTAVEPYKASSFDLTHKLTVEKHGHTALITINHPPANTWDRDSLIGLRQLIEHLNRDDDIYALVVTGQGPKFFSAGADLNMFADGDKARAREMARRFGE AFEALRDFRGVSIAAINGYAMGGGLECALACDIRIAERQAQMALPEAAVGLLPCAGGTQALPWLVGEGWAKRMILCNERVDAETALRIGLVEQVVDSGEA RGAALLLAAKVARQSPVAIRTIKPLIQGARERAPNTWLPEERERFVDLFDAQDTREGVNAFLEKRDPKWRNC

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0745 gene of Pseudomonas aeruginosa PAO1, Chromosome (812445 to 813263 bp)

Displaying the first five SNPs out of a total of 29 found in all isolate strains ( View all 29 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa PA7	Transition	g.812523A>G		Silent GAT(Asp) -> GAC(Asp)	SNP-264484	View
Pseudomonas aeruginosa C3719	Transition	g.812556A>G		Silent ACT(Thr) -> ACC(Thr)	SNP-21881	View
Pseudomonas aeruginosa 2192	Transition	g.812556A>G		Silent ACT(Thr) -> ACC(Thr)	SNP-66048	View
Pseudomonas aeruginosa LESB58	Transition	g.812556A>G		Silent ACT(Thr) -> ACC(Thr)	SNP-92549	View
Pseudomonas aeruginosa PACS2	Transition	g.812556A>G		Silent ACT(Thr) -> ACC(Thr)	SNP-100701	View

Select to view SNPs in a specific strain

SNP Map

PubMed