Pseudomonas Genome Database - Annotation for PA0747

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PA0747

Key to diagram

View in GBrowse:

15 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Operons/Regulons
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name		Alternate Gene Names
Locus tag	PA0747 (View 7 updates) Submit an Update	GenBank Identifier (GI)	15595944 (Precomputed BLAST results at BLINK )
Genomic location	816012 - 814504 (-)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG000721 Possible inparalog involved. Orthology uncertain because more than one top BLASTp hit was identified.
Product Information
Product Name	probable aldehyde dehydrogenase (Class 3 )
Alternate Product Names
Subcellular localization	Cytoplasmic (Class 1) Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA00748
Systomonas	PA0747
RefSeq	NP_249438.1
GI	15595944
KEGG	PA0747
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Operons/Regulons

Type

operon

Genes

Gene	Product
PA0746	probable acyl-CoA dehydrogenase
PA0747	probable aldehyde dehydrogenase

Evidence

Computationally Predicted

PubMed

18988623

Cross-Reference

DOOR-13270

Genomic Context

Genomic context

- The two downstream genes encode a probable acyl-CoA dehydrogenase and a probable enoyl-CoA hydratase.

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Putative enzymes

GO Accession

GO Term

Evidence

Molecular Function

GO:0004491

methylmalonate-semialdehyde dehydrogenase (acylating) activity

IEA

Biological Process

GO:0006574

valine catabolic process

IEA

Cellular Component

COG Prediction

Accession	Description	Category
COG1012	PutA, NAD-dependent aldehyde dehydrogenases [Energy production and conversion].	Energy production and conversion

Pathways

Name	Database	Xref
Valine, leucine and isoleucine degradation	KEGG	pae00280	Computationally Generated
Inositol phosphate metabolism	KEGG	pae00562	Computationally Generated
Propanoate metabolism	KEGG	pae00640	Computationally Generated
Metabolic pathways	KEGG	pae01100	Computationally Generated

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

80% similar to methylmalonate semialdehyde dehydrogenase MmsA [Pseudomonas aeruginosa]

Most Similar Human Homolog

ENSP00000342564, Percent Identity: 54.70%, E-Value: 6e-155, Ensembl Release: 56.
Description: Methylmalonate-semialdehyde dehydrogenase

Structure Features

PS00687: Aldehyde dehydrogenases glutamic acid active site

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF00171	Aldedh, Aldehyde dehydrogenase family.	13	477	1e-138	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

AGGAAATCCTCCGCCAGTCCGCCTTCGAAATGTACCCAGTAGATGCTCCAGGGCCGCTGCGAGTCGGCGCCGTAGGCATGTTCCCGGCCCTTGGGCAGGA CCAGCAGGTCGCCGCCGCCGACCTCGACGCGCTCGCCGGCACACTCCAGCCAGCCGCGTCCGGCCCGGCAGTAGATCAACAGGTTGTCGTCCGGCGCGCG CCGCTGCATGTGGTGGCCATGCGCCTCCGGATAGTAACCGAGGGCCAGCGGGTAGCAGGCCTGGCCGAGCGGATGGCGGGCCAGGGCACGACGCAGGCGG GGCGGCGTGATGAAACGCACCCCCTGGTTGGGCAGGGGCCAGGCGGAGGTCTGTACGGAACGCTTCACGGCGACGCGACTCGGTAGTCTGAATCCCAAGA TCGTCCATCCCTTGCGCAAGATCGTCAATCCACAGGCGGCTTGCGGCCGGCTATAACGACTCAAAACAACATCCCGGTCCACCGGGAAGCGGAGGCGACG

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGGCCAAGGCAATCCCCCAGCTGATCGACGGCGAATGGCGCGGCAGCCGTTCCCGCGAGCTGGTCGAAGTCACCGATCCGGCGACCCAGGACGTCCTCG CGCTGGCGCCGAAGGCCACCGCCGACGAGATCGAGCAGGCCATCGCCAGCGCCCAGCGCGCCTTCGAGACCTGGCGCGAAGTCCCGGCTCCCGAGCGAGC GCGGTTGATGCTGCGCTACCAGCACCTGCTCAAGGAACACCACGACGAACTCGGCGAACTGCTGGCCCGGGAGACCGGCAAGAACCTCGCCGATGCCAAG GGCGACGTGTGGCGCGGCATCGAGGTGGTCGAACACGCCGCCAACGTGGCCAGTCTGATGATGGGCGAGACGGTGGAAAACGTCGCCCGCGAGATCGACA CCGCCAGTTGGATCCAGCCCCTCGGTGTGTGCGCCGGGATCACCCCGTTCAATTTCCCGGCGATGATCCCGCTGTGGATGTTCCCGCTGGCCATCGCCTG CGGCAACACCTTCGTCCTCAAGCCCTCCGAACAGGATCCGCTGACACCCAACCGCCTCGCCGAGCTGTTCCTCGAGGCCGGCGCGCCGAAGGGCGTGCTG CAGGTGATTCATGGCGGCCGCGAGCAGGTCGATGCCCTCCTTACCCACCCGGACATCCGGGCGATTTCCTTCGTCGGCTCGGTCGCCGTCGGCCAGCACG TGTACCGCACCGGCACCGCGCACCTGAAGCGGGTGCAGGCCTTTGCCGGGGCGAAGAACCACATGGTGATCCTCCCCGACGCGAACAAGGCCCAGGTTCT CGGCAACCTGGTCGGCGCCAGTTGCGGCGCCGCCGGCCAGCGCTGCATGGCGATCAGCGCGGCGGTGTTCGTCGGCGCGGCGCGCGAGTGGATTCCCGAA CTGGCCGAGCGGATGGCCGTCCTGCGCCCCGGCCACTGGCAGGACCCGGACGCCGCCTATGGTCCGCTGATCAGCCCGCAGGCGCGCCAGCGCGTGCTGC GCCTGATCGCCGAAGGCAAGGCGGAAGGCGCCGAGTGCCTGCTCGACGGCTCGCAGTGCCAGGTCGAAGGCTATCCCAACGGCAACTGGCTCGGTCCCAC CCTGTTCCGCGCAGTGACGCCGAAGATGGGCCTGTACCGCGAGGAAATCTTCGGCCCGGTGCTGGCCTGCCTGGAGGTGGAAAGCCTGGAAGAGGCCATC GCCCTGGTCAACGCCAACCCCTATGGCAATGGAACAAGCCTGTTCACCCGCTCCGGCGGCGCCGCTCGGCGCTACCAGCACGCGGTGCAGGTCGGCCAGG TGGGTATCAACGTGCCGATCCCGGTACCGCTGCCGTTCTTCTCCTTCACCGGCTGGAAGGGTTCCTTCTACGGCGACCTGCACGCCTACGGCAAGCAGGC GGTGCGCTTCTATACCGAGACCAAGACCGTCACCAGCCGCTGGTTCGACGATGAACCGACGACCGACGAATCGTCGGCCGGCCCGAACATGACCATCCAT CTCAAGTGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MAKAIPQLIDGEWRGSRSRELVEVTDPATQDVLALAPKATADEIEQAIASAQRAFETWREVPAPERARLMLRYQHLLKEHHDELGELLARETGKNLADAK GDVWRGIEVVEHAANVASLMMGETVENVAREIDTASWIQPLGVCAGITPFNFPAMIPLWMFPLAIACGNTFVLKPSEQDPLTPNRLAELFLEAGAPKGVL QVIHGGREQVDALLTHPDIRAISFVGSVAVGQHVYRTGTAHLKRVQAFAGAKNHMVILPDANKAQVLGNLVGASCGAAGQRCMAISAAVFVGAAREWIPE LAERMAVLRPGHWQDPDAAYGPLISPQARQRVLRLIAEGKAEGAECLLDGSQCQVEGYPNGNWLGPTLFRAVTPKMGLYREEIFGPVLACLEVESLEEAI ALVNANPYGNGTSLFTRSGGAARRYQHAVQVGQVGINVPIPVPLPFFSFTGWKGSFYGDLHAYGKQAVRFYTETKTVTSRWFDDEPTTDESSAGPNMTIH LK

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0747 gene of Pseudomonas aeruginosa PAO1, Chromosome (814504 to 816012 bp)

Displaying the first five SNPs out of a total of 47 found in all isolate strains ( View all 47 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa 2192	Transversion	g.814515G>C		Not determined CAT(His) -> CGA()	SNP-66056	View
Pseudomonas aeruginosa PA7	Transition	g.814551T>C	p.Thr488Ala	Missense ACC(Thr) -> GCC(Ala)	SNP-264519	View
Pseudomonas aeruginosa C3719	Transversion	g.814612C>A		Silent GCG(Ala) -> GCT(Ala)	SNP-21887	View
Pseudomonas aeruginosa 2192	Transversion	g.814612C>A		Not determined GCG(Ala) -> TGT()	SNP-66057	View
Pseudomonas aeruginosa PA14	Transition	g.814651A>G		Silent GGT(Gly) -> GGC(Gly)	SNP-160711	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Characterization of the mmsAB operon of Pseudomonas aeruginosa PAO encoding methylmalonate-semialdehyde dehydrogenase and 3-hydroxyisobutyrate dehydrogenase.
Steele MI, Lorenz D, Hatter K, Park A, Sokatch JR.
J Biol Chem. 1992 Jul 5;267(19):13585-92.
PMID: 1339433
PSORTb 3.0: Improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes.
Yu NY, Wagner JR, Laird MR, Melli G, Rey S, Lo R, Dao P, Sahinalp SC, Ester M, Foster LJ, Brinkman FS.
Bioinformatics. 2010 May 13; [Epub ahead of print]
PMID: 20472543