Pseudomonas Genome Database - Annotation for PA0835

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PA0835

Key to diagram

View in GBrowse:

16 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Operons/Regulons
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name	pta	Alternate Gene Names
Locus tag	PA0835 (View 7 updates) Submit an Update	GenBank Identifier (GI)	15596032 (Precomputed BLAST results at BLINK )
Genomic location	911532 - 909418 (-)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG000803
Product Information
Product Name	phosphate acetyltransferase (Class 1 )
Alternate Product Names	phosphotransacetylase
Subcellular localization	Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA00836
Systomonas	PA0835
RefSeq	NP_249526.1
GI	15596032
KEGG	PA0835
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Operons/Regulons

Type

operon

Genes

Gene	Product
pta	phosphate acetyltransferase
ackA	acetate kinase

Evidence

Computationally Predicted

PubMed

18988623

Cross-Reference

DOOR-13285

Genomic Context

Genomic context

- like E. coli
- Adjacent to probable ackA gene

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Carbon compound catabolism

GO Accession

GO Term

Evidence

Molecular Function

GO:0008137	NADH dehydrogenase (ubiquinone) activity	ISS
GO:0008959	phosphate acetyltransferase activity	IEA

Biological Process

GO:0044248	cellular catabolic process	ISS
GO:0015978	carbon utilization by utilization of organic compounds	ISS
GO:0006090	pyruvate metabolic process	ISS
GO:0006086	acetyl-CoA biosynthetic process from pyruvate	IEA

Cellular Component

COG Prediction

Accession	Description	Category
COG0280	Pta, Phosphotransacetylase [Energy production and conversion].	Energy production and conversion

Pathways

Name	Database	Xref
Pyruvate metabolism	PseudoCAP
Taurine and hypotaurine metabolism	KEGG	pae00430	Computationally Generated
Pyruvate metabolism	KEGG	pae00620	Computationally Generated
Propanoate metabolism	KEGG	pae00640	Computationally Generated

Reactions

EC Number

2.3.1.8

Enzyme Assays

PDF file(s) provided by Sigma-Aldrich:
Phosphotransacetylase (2.3.1.8)

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

71% similar to pta gene product from [E. coli]

Structure Features

PD02355: Acyltransferase signature

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF07085	DRTGG, DRTGG domain.	223	334	2e-32	23.0
PF01515	PTA_PTB, Phosphate acetyl/butaryl transferase.	380	695	1e-111	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

GCGCGGACCCTGGGCTGGAGCCTGGAGCGCATCGACTCGATGCTGAACAACGAAAGCGGCCTGCTCGGCCTCTCCGACCTGTCCAACGACATGCGCACCC TGGAGCAGGAGCGCGAGCAGGGCCACCCCGGCGCGGCCCTGGCGATCGAGGTGTTCTGCTACCGCCTGGCCAAGTCCCTGGCGGCGATGAGCTGCGCCCT GCCGCAACTGGACGGGGTGATCTTCACCGGTGGCATCGGCGAGAACTCGCCGCTGGTGCGCGCCAAGACCGCCGCCCACCTGCGGCTGTTCGACCTGCGC CTCGACCAGGAGGCCAACGCCCGCTGCGTGCGCGGCGTCGCCGGGCCGATCCAGGCCGCGGGACATCCGCGGGTACTGGTGATCCCGACCAACGAAGAGC GGCAGATCGCCCTCGACACGCTGGCCCTGCTCGACTGAACTGGATCGACGCCCCCGCCCAACCAGGCGGTGAGGCCAAACGCACGGCTTAAGGAGCCCAG

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGCATACCTTCTTCATCGCCCCGACCGGTTTCGGCGTCGGCCTCACCTCGATCAGCCTCGGCCTGCTTCGCGCCCTGGAACGCGCCGGGCTGAAGGTCG GCTTCTTCAAACCCATCGCCCAACTGCATCCCGGCGACCTCGGCCCGGAGCGCTCCAGCGAACTGGTGGCGCGCACCCACGGCCTGGATACGCCGAAGCC GCTGCCGCTGGCCCAGGTCGAGCGCATGCTCGGCGACGGCCAACTGGACGAACTGCTCGAAGAGATCATCAGCCTCTACCAGCGCGCCGCCGCCGACAAG GACGTGGTGATCGTCGAAGGCATGGTACCGACCCGTCACGCCAGCTACGCCGCGCGGGTCAACTTCCACCTGGCAAAGAGTCTCGACGCCGAGGTGATCC TGGTCTCCGCGCCGGAAAACGAGACGCTCACCGAACTGACCGACCGTATCGAGATCCAGGCCCAGCTGTTCGGCGGCCCGCGCGATCCGAAGGTGCTCGG GGTGATCCTCAACAAGGTCCGCGGCGAGGCCGACGCGGCCAACGCCGAGGACGGCGTGGCGGACTTCGCCCGGCGCCTCACCGAACACTCGCCGCTGCTG CGCGACGACTTCCGCCTGATCGGCTGCATTCCCTGGCAGGACGAACTCAACGCCGCGCGCACCCGCGACATCGCCGACCTGCTCAGCGCACGGGTGATCA ACGCCGGCGACTATGAGCAGCGCCGGGTGCAGAAGATCGTGCTATGCGCCCGCGCCGTGCCCAACACCGTGCAACTGCTCAAGCCCGGCGTGCTGGTGGT GACCCCCGGCGACCGCGACGACATCATCCTCGCCGCCAGCCTGGCCGCGATGAACGGCGTACCCTTGGCCGGCCTGCTGCTGTGCAGCGACTTCCCGCCC GACCCGCGGATCATGGAACTCTGCCGCGGCGCCCTGCAGGGCGGCCTGCCGGTGCTGTCGGTGGCCACCGGCTCCTACGACACCGCGACCAACCTGAACC GGATGAACAAGGAAATCCCGGTGGACGACCGCGAGCGCGCCGAGCGGGTCACCGAGTTCGTCGCCGGGCACATCGATTTCGAATGGCTCAAGCAGCGCTG CGGCACGCCGCGCGAGCTGCGCCTGTCGCCGCCGGCGTTCCGCTACCAGGTGGTGCAACGCGCGCAGAAGGCCGGCAAGCGCATCGTCCTGCCCGAGGGC AGCGAGCCGCGCACCGTACAGGCGGCGGCGATCTGCCAGGCGCGCGGCATCGCCCGCTGCGTGCTGCTGGCCAAGCCGGAAGAGGTCCAGGCGGTGGCCC AGGCCCAGGGCATCGTGCTACCGGAAGGCCTGGAGATCATCGACCCTGACCTGGTGCGGCAGCGCTACGTCGAACCGATGGTCGAGCTGCGCAAGGGCAA GGGGCTGAACGCGCCGATGGCGGAGCAGCAACTGGAGGACAGCGTGGTCCTGGCGACCATGATGCTTGCCCTGGACGAGGTCGACGGCCTGGTCTCCGGC GCCATCCACACCACCGCCAGCACCATCCGCCCGGCGCTGCAACTGATCAAGACCGCGCCCGGCTACAACCTGGTGTCGTCAGTGTTTTTCATGTTGTTAC CGGACCAGGTGCTGGTCTACGGCGATTGCGCGGTGAATCCCGATCCTTCGGCCAGTGACCTGGCCGAGATCGCCGTGCAGAGTGCCGCGTCGGCGCAGGC TTTCGGCATCCCGGCACGGGTCGCGATGATCAGCTACTCCACCGGCGACTCCGGTTCCGGGGTGGATGTCGACAAGGTTCGCGAGGCCACCCGCCTGGCG CGCGAGCAGCGCCCCGACCTGTTGATCGACGGACCCTTGCAGTATGATGCCGCCGCCATCGCCAGCGTCGGCCGCCAGAAGGCGCCGAACAGCCCGGTGG CCGGGCAGGCCACGGTGTTCATCTTCCCCGACCTGAACACCGGCAACACCACCTACAAGGCGGTGCAGCGCAGCGCCGATTGCGTCAGCGTCGGGCCGAT GCTGCAAGGCCTGCGCAAACCGGTGAACGACCTGTCGCGCGGCGCGCTGGTGGAAGACATCGTCTACACCATCGCCCTCACCGCCATCCAGGCGGACGCC CAGGCCCCGGCCTGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MHTFFIAPTGFGVGLTSISLGLLRALERAGLKVGFFKPIAQLHPGDLGPERSSELVARTHGLDTPKPLPLAQVERMLGDGQLDELLEEIISLYQRAAADK DVVIVEGMVPTRHASYAARVNFHLAKSLDAEVILVSAPENETLTELTDRIEIQAQLFGGPRDPKVLGVILNKVRGEADAANAEDGVADFARRLTEHSPLL RDDFRLIGCIPWQDELNAARTRDIADLLSARVINAGDYEQRRVQKIVLCARAVPNTVQLLKPGVLVVTPGDRDDIILAASLAAMNGVPLAGLLLCSDFPP DPRIMELCRGALQGGLPVLSVATGSYDTATNLNRMNKEIPVDDRERAERVTEFVAGHIDFEWLKQRCGTPRELRLSPPAFRYQVVQRAQKAGKRIVLPEG SEPRTVQAAAICQARGIARCVLLAKPEEVQAVAQAQGIVLPEGLEIIDPDLVRQRYVEPMVELRKGKGLNAPMAEQQLEDSVVLATMMLALDEVDGLVSG AIHTTASTIRPALQLIKTAPGYNLVSSVFFMLLPDQVLVYGDCAVNPDPSASDLAEIAVQSAASAQAFGIPARVAMISYSTGDSGSGVDVDKVREATRLA REQRPDLLIDGPLQYDAAAIASVGRQKAPNSPVAGQATVFIFPDLNTGNTTYKAVQRSADCVSVGPMLQGLRKPVNDLSRGALVEDIVYTIALTAIQADA QAPA

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0835 gene of Pseudomonas aeruginosa PAO1, Chromosome (909418 to 911532 bp)

Displaying the first five SNPs out of a total of 90 found in all isolate strains ( View all 90 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa PA7	Transversion	g.909427G>C		Silent GCC(Ala) -> GCG(Ala)	SNP-257084	View
Pseudomonas aeruginosa PA7	Transition	g.909571C>T		Silent GCG(Ala) -> GCA(Ala)	SNP-257085	View
Pseudomonas aeruginosa PA7	Transversion	g.909583G>C		Silent ACC(Thr) -> ACG(Thr)	SNP-257086	View
Pseudomonas aeruginosa PA7	Transversion	g.909721G>T		Silent CGC(Arg) -> CGA(Arg)	SNP-257087	View
Pseudomonas aeruginosa PA7	Transition	g.909754A>G		Silent GTT(Val) -> GTC(Val)	SNP-257088	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Long-term anaerobic survival of the opportunistic pathogen Pseudomonas aeruginosa via pyruvate fermentation.
Eschbach M, Schreiber K, Trunk K, Buer J, Jahn D, Schobert M.
J Bacteriol. 2004 Jul;186(14):4596-604.
PMID: 15231792
Identification and characterization of the ackA (acetate kinase A)-pta (phosphotransacetylase) operon and complementation analysis of acetate utilization by an ackA-pta deletion mutant of Escherichia coli.
Kakuda H, Hosono K, Shiroishi K, Ichihara S.
J Biochem. 1994 Oct;116(4):916-22.
PMID: 7883769