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               PA0836               
Key to diagram


View in GBrowse: 6 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name ackA  Alternate Gene Names tdcD
Locus tag PA0836 (View 8 updates) Submit an Update GenBank Identifier (GI) 15596033 (Precomputed BLAST results at BLINK )
Genomic location 912779 - 911595 (-) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name acetate kinase (Class 2  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Cytoplasmic (Class 2Explanation of Localization Confidence)
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA00837
Systomonas PA0836
RefSeq NP_249527.1
GI 15596033
KEGG PA0836
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
pta phosphate acetyltransferase
ackA acetate kinase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13285
Genomic Context
Genomic context Explanation of Genomic Context Field - Adjacent to pta gene, like ackA gene in E. coli
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Putative enzymes
GO Accession GO Term Evidence
Molecular Function
GO:0008776 acetate kinase activity IEA
Biological Process
GO:0006083 acetate metabolic process IEA
GO:0006113 fermentation IEA
Cellular Component
GO:0005737 cytoplasm IEA
COG PredictionExplanation of COG predictions
Accession Description Category
COG0282 ackA, Acetate kinase [Energy production and conversion]. Energy production and conversion
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Pyruvate metabolism PseudoCAP
carbon monoxide dehydrogenase pathway PseudoCyc CODH-PWY
acetate degradation PseudoCyc ACETATEUTIL-PWY
lactate oxidation PseudoCyc LCTACACAT-PWY
sorbitol metabolism PseudoCyc P461-PWY
fermentation PseudoCyc FERMENTATION-PWY
pyruvate metabolism PseudoCyc P41-PWY
pyruvate oxidation pathway PseudoCyc PYRUVOX-PWY
Taurine and hypotaurine metabolism KEGG pae00430 Computationally Generated
Pyruvate metabolism KEGG pae00620 Computationally Generated
Propanoate metabolism KEGG pae00640 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 2.7.2.1
Enzyme Assays
PDF file(s) provided by Sigma-Aldrich:
 Acetate Kinase Bacillus stearothermophilus (2.7.2.1)
PDF file(s) provided by Sigma-Aldrich:
 Acetate Kinase (2.7.2.1)
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 63% similar to both tdcD and ackA gene products from E. coli
Structure Features
Structure Features Explanation of Structure Features Field PS01075: Acetate and butyrate kinases family signature 1
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00871 Acetate_kinase, Acetokinase family. 6 386 1e-124 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region TCCGGCTCGATGGCGACGCTCAGTTCGTCACCGACGTTCTTGCCTTCCAGGGCCTTCTCCAGGCCGACGATGATGTTGCCGGCACCGTGCAGGTAGACCA GCGGCGCGCCACCAGCGGAACTGTCGATGACATCCCCGGCGTCGTTGGTCAGGGTATAGTCGATGGAAACCGCCTTGTTGGCCGCGATCTGCATGGTTCG AAACCTTTTCGATAAAGAAGATGAATGCGTAAGTGTACGCATCAATAGCCCCGAAAGCGACCGCGCCCCTGATGAACGGGGCGCGCGAGCGCCGTCGCGG GTGATTGATTTTCATCAGGACGAGGAAGGCCATTGGGTGGCAGTCTTGTCCTGTGGCCATACTCAACACCTGCGCCACCAACCGCCCTGGCAATCGCGCG CCTGGGTCCTTGACGAAAACGAGCGGCACAGACAGCTCGGCCGCGAATTTCGCTGCGGCTGGTGTGCCCGGGAAGAAGAAGAGCAGACCAAGGAGCAGTG

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGCCCTCACGCAACATACTGGTGATCAACTGCGGCAGTTCGTCGATCAAGTTCGCCCTGGTCAACGAGGCCCACTCCCTGTTTCCCCTGCACGGCCTCG CCGAGCGCCTGGGCAGCCGCGATGCGGTGCTGCGCTGGAAGCGCGGCGGCGACAGCGACAGCCTGATGATTCCCAACGCCGACCACCGCGCCGCCCTCGC CCAGTTGCTGCCGATGGTGCAGAACGCCGCGGGCGGCAAGCTCCACGGCATCGGCCACCGGGTGGTGCATGGCGGCGAGCTGTTCACCCATGCCACGCGC ATCGACGACCGGGTGGTCGAGGCGATCCGGGCCACCGCGCCGCTGGCGCCGCTGCACAACCCGGCCAACCTGCAAGGCATCGAGGCAGCGATGACGCTGT TTCCCAAGCTGCCCCACGTCGCCGTGTTCGACACCGCCTTCCACCAGAGCCTGCCGGAGCACGCCTACCGCTACGCCCTGCCGGAGGCCCTCTACCGTGA GCATGGCGTGCGCCGCTACGGCTTCCACGGCACCAGCCACCGCTACGTCAGCCACCGCGCCGCGGAAATGGCCGGGTTGGCGGTCGGCGACAGCAGTTGG CTCAGCGCCCACCTCGGCAACGGCAGCTCGACCTGCGCCATCGTCAACGGCCAGAGCCTCGACACCAGCATGGGCCTGACCCCGCTGGAAGGCCTGGTAA TGGGCACCCGCAGCGGCGACGTCGACCCCAACCTGCACAGCCACCTGGCGCGGACCCTGGGCTGGAGCCTGGAGCGCATCGACTCGATGCTGAACAACGA AAGCGGCCTGCTCGGCCTCTCCGACCTGTCCAACGACATGCGCACCCTGGAGCAGGAGCGCGAGCAGGGCCACCCCGGCGCGGCCCTGGCGATCGAGGTG TTCTGCTACCGCCTGGCCAAGTCCCTGGCGGCGATGAGCTGCGCCCTGCCGCAACTGGACGGGGTGATCTTCACCGGTGGCATCGGCGAGAACTCGCCGC TGGTGCGCGCCAAGACCGCCGCCCACCTGCGGCTGTTCGACCTGCGCCTCGACCAGGAGGCCAACGCCCGCTGCGTGCGCGGCGTCGCCGGGCCGATCCA GGCCGCGGGACATCCGCGGGTACTGGTGATCCCGACCAACGAAGAGCGGCAGATCGCCCTCGACACGCTGGCCCTGCTCGACTGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MPSRNILVINCGSSSIKFALVNEAHSLFPLHGLAERLGSRDAVLRWKRGGDSDSLMIPNADHRAALAQLLPMVQNAAGGKLHGIGHRVVHGGELFTHATR IDDRVVEAIRATAPLAPLHNPANLQGIEAAMTLFPKLPHVAVFDTAFHQSLPEHAYRYALPEALYREHGVRRYGFHGTSHRYVSHRAAEMAGLAVGDSSW LSAHLGNGSSTCAIVNGQSLDTSMGLTPLEGLVMGTRSGDVDPNLHSHLARTLGWSLERIDSMLNNESGLLGLSDLSNDMRTLEQEREQGHPGAALAIEV FCYRLAKSLAAMSCALPQLDGVIFTGGIGENSPLVRAKTAAHLRLFDLRLDQEANARCVRGVAGPIQAAGHPRVLVIPTNEERQIALDTLALLD

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0836 gene of Pseudomonas aeruginosa PAO1, Chromosome (911595 to 912779 bp)


Displaying the first five SNPs out of a total of 90 found in all isolate strains ( View all 90 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA7 Transversion g.911613C>G
Silent
ACG(Thr) -> ACC(Thr) 		SNP-257155 View
Pseudomonas aeruginosa PA7 Transversion g.911652C>G
Silent
GTG(Val) -> GTC(Val) 		SNP-257156 View
Pseudomonas aeruginosa PA7 Transition g.911658T>C
Silent
GTA(Val) -> GTG(Val) 		SNP-257157 View
Pseudomonas aeruginosa C3719 Transition g.911667A>G
Silent
CAT(His) -> CAC(His) 		SNP-9174 View
Pseudomonas aeruginosa PA7 Transition g.911667A>G
Silent
CAT(His) -> CAC(His) 		SNP-257158 View
Select to view SNPs in a specific strain
SNP Map
PubMed Long-term anaerobic survival of the opportunistic pathogen Pseudomonas aeruginosa via pyruvate fermentation.
Eschbach M, Schreiber K, Trunk K, Buer J, Jahn D, Schobert M.
J Bacteriol. 2004 Jul;186(14):4596-604.
PMID: 15231792
Regulation of the Bacillus subtilis acetate kinase gene by CcpA.
Grundy FJ, Waters DA, Allen SH, Henkin TM.
J Bacteriol. 1993 Nov;175(22):7348-55.
PMID: 8226682
Novel keto acid formate-lyase and propionate kinase enzymes are components of an anaerobic pathway in Escherichia coli that degrades L-threonine to propionate.
Hesslinger C, Fairhurst SA, Sawers G.
Mol Microbiol. 1998 Jan;27(2):477-92.
PMID: 9484901
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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