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               PA0854               
Key to diagram


View in GBrowse: 16 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name fumC2  Alternate Gene Names
Locus tag PA0854 (View 7 updates) Submit an Update GenBank Identifier (GI) 15596051 (Precomputed BLAST results at BLINK )
Genomic location 934112 - 932718 (-) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name fumarate hydratase (Class 2  Explanation of Product Name Confidence)
Alternate Product Names fumarase
Subcellular localization
Cytoplasmic (Class 2Explanation of Localization Confidence)
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA00855
Systomonas PA0854
RefSeq NP_249545.1
GI 15596051
KEGG PA0854
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
PA0853 probable oxidoreductase
fumC2 fumarate hydratase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13289
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Energy metabolism
Carbon compound catabolism
GO Accession GO Term Evidence
Molecular Function
GO:0004333 fumarate hydratase activity IEA
Biological Process
GO:0044248 cellular catabolic process ISS
GO:0015978 carbon utilization by utilization of organic compounds ISS
GO:0006091 generation of precursor metabolites and energy ISS
GO:0006099 tricarboxylic acid cycle IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0114 FumC, Fumarase [Energy production and conversion]. Energy production and conversion
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Citrate cycle (TCA cycle) PseudoCAP
phenylalanine degradation I PseudoCyc PHENYLALANINE-DEG1-PWY
fermentation PseudoCyc FERMENTATION-PWY
glycolysis+TCA+glyoxylate bypass PseudoCyc GLYCOLYSIS-TCA-GLYOX-BYPASS
acetyl-CoA assimilation PseudoCyc P22-PWY
anaerobic respiration PseudoCyc ANARESP1-PWY
TCA cycle -- aerobic respiration PseudoCyc TCA
glyoxylate bypass+TCA PseudoCyc TCA-GLYOX-BYPASS
Citrate cycle (TCA cycle) KEGG pae00020 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 4.2.1.2
Enzyme Assays
PDF file(s) provided by Sigma-Aldrich:
 Fumarase (4.2.1.2)
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 75% similar to fumC gene product [E. coli]
Most Similar Human Homolog
ENSP00000355518, Percent Identity: 59.17%, E-Value: 3e-140, Ensembl Release: 56.
Description: Fumarate hydratase, mitochondrial Precursor
Structure Features
Structure Features Explanation of Structure Features Field PS00163: Fumarate lyases signature
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00206 Lyase_1, Lyase. 10 340 9e-94 23.0
PF10415 FumaraseC_C, Fumarase C C-terminus. 406 459 1e-22 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region TGGACCCGCGCCGCCTTCGCCGCCCCCGGCGGCATCGCCGGCAACTTCGGCACCGGCTGGACCGAAGCCAGCGACCTGCGCGAGAACGCCCTGTGGTTCG GCGGCAAGCTCAGCCGCGTGCTCGACGACGTGCACATCCGCGAGCCTCGCGACCCGCTGGCCGAATGGCGCCTGGACAGCGCCTGCGGTCGCGTCGAGCT GCTCTTCCGTCCCGAACAGCTGCACCAGGCGCGGCCCAGCGTCGGCCTGTTCTATGCCAATACCCGCCAGTGGTTCGGCCGTTTCAACGGCACCCTGCGC CACGACGACGGCGACTGCGTGCCGGTGGACGGCGCCCTCGGCTGGATCGGTTCGACCCGCGCGCGCTGGTGAGGCCGGCGGCGGGCAATCTATTTCCGAC CCGCAGCGCGGCGAAAAATGCCCACGAGGAAGCGACGGCAGCGGAACCCAAGGCGGGAGCGAAGGCCTAAACTGCCGGATACGCACCCTGGAGAACACTC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGAGCCGGACCGAAACCGATAGCCTGGGCACCATCGAGGTGCCCGACGACGCCTACTGGGGCGCCCAGACCCAGCGTTCGCTGGAGAACTTCGCCATCG GCGGCCAGCGCATGCCGCTCGCCGTGATCCACGCCCTGGCGCTGATCAAGAAAGCCGCCGCGCGGGTCAACGACCACCTCGGCGAACTGCCGCCGGAAGT CGCCCGGCTGATCGAACAGGCCGCCGACGAAGTCCTCGCAGGCCGCCACGACGAACACTTCCCGCTGGTGGTCTGGCAGACCGGCAGCGGCACCCAGACC AACATGAACGTCAACGAGGTGATCGCTGGCCGCGCCAACGAACTGGCCGGCAATCCGCGTGGCGGCAAGTCGCCGGTGCATCCCAACGACCACGTCAACC GCGCGCAGAGTTCCAACGACAGCTTCCCCACCGCCATGCACATCGCCGCCGCCAAGGCCGTGCACGAACAATTGCTGCCGGCCATCGCCGAGCTTTCCGG CGGTCTCGCCGAACAGTCGGCGCGGCACGCCAGCCTGGTCAAGACCGGGCGCACCCACCTGATGGACGCCACGCCGATCACCTTCGGCCAGGAACTCTCG GCCTTCGTCGCCCAGCTCGACTACGCCGAGCGCGCCATCCGCGCGGCGCTGCCGGCGGTCTACCAGTTGGCCCAGGGCGGCACCGCGGTGGGCACCGGGC TCAACGCGCCGAAGGGCTTCGCCGACGCCATCGCCGCGGAAATCGCCGCCGAATCGGGCCTGCCCTTCGTCGCCGCGCCGAACAAGTTCGCCGCCCTGGC CGGCCACGAGCCGCTGGTCATCCTCTCCGGCGCCCTGAAGAGCCTGGCGGTGGCCCTGATGAAGATCGCCAACGACCTGCGCCTGCTCGGTTCCGGCCCA CGCGCCGGCTTCGCCGAGGTGAAGCTGCCGGCCAACGAGCCGGGCAGCTCGATCATGCCCGGCAAGGTCAACCCGACCCAGTGCGAGGCGCTGTCGATGC TGGCCTGCCAGGTCATGGGCAACGACAGCACGATTTCCTTCGCCGCCAGCCAGGGCCATCTGCAACTCAACGTGTTCAAGCCGGTGATCGTCTACAACCT GCTGGAGTCCATCCGCCTGCTCGCCGACGGCTGCCGCAACTTCAACAAGCATTGCGTCGCCGGCCTGGAGCCGGACGCCCAGCGCATGGCCGACCTGCTG GAGCGCGGCCTGATGCTGGTCACCGCACTGAACCCGCACATCGGCTACGACAAGGCCGCCGAAATCGCCAAGAAGGCCTACGCCGAAGGCACCACCCTGC GCGCCGCGGCGCTGCAGCTGGGCTACCTGGACGAAGCGCAGTTCGACGAATGGGTACGGCCGGAGCAGATGCTGGAGGCCGGCCACCATGGTTGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MSRTETDSLGTIEVPDDAYWGAQTQRSLENFAIGGQRMPLAVIHALALIKKAAARVNDHLGELPPEVARLIEQAADEVLAGRHDEHFPLVVWQTGSGTQT NMNVNEVIAGRANELAGNPRGGKSPVHPNDHVNRAQSSNDSFPTAMHIAAAKAVHEQLLPAIAELSGGLAEQSARHASLVKTGRTHLMDATPITFGQELS AFVAQLDYAERAIRAALPAVYQLAQGGTAVGTGLNAPKGFADAIAAEIAAESGLPFVAAPNKFAALAGHEPLVILSGALKSLAVALMKIANDLRLLGSGP RAGFAEVKLPANEPGSSIMPGKVNPTQCEALSMLACQVMGNDSTISFAASQGHLQLNVFKPVIVYNLLESIRLLADGCRNFNKHCVAGLEPDAQRMADLL ERGLMLVTALNPHIGYDKAAEIAKKAYAEGTTLRAAALQLGYLDEAQFDEWVRPEQMLEAGHHG

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0854 gene of Pseudomonas aeruginosa PAO1, Chromosome (932718 to 934112 bp)


Displaying the first five SNPs out of a total of 61 found in all isolate strains ( View all 61 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA14 Transition g.932727G>A
Silent
CAC(His) -> CAT(His) 		SNP-181157 View
Pseudomonas aeruginosa PA7 Transversion g.932746T>A p.Gln456Leu
 Missense
CAG(Gln) -> CTG(Leu) 		SNP-258570 View
Pseudomonas aeruginosa PA7 Transition g.932748C>T
Silent
GAG(Glu) -> GAA(Glu) 		SNP-258571 View
Pseudomonas aeruginosa PA7 Transversion g.932754C>A
Silent
CGG(Arg) -> CGT(Arg) 		SNP-258572 View
Pseudomonas aeruginosa PA7 Transition g.932757T>C
Silent
GTA(Val) -> GTG(Val) 		SNP-258573 View
Select to view SNPs in a specific strain
SNP Map
PubMed Two biochemically distinct classes of fumarase in Escherichia coli.
Woods SA, Schwartzbach SD, Guest JR.
Biochim Biophys Acta. 1988 Apr 28;954(1):14-26.
PMID: 3282546
Crystallographic studies of the catalytic and a second site in fumarase C from Escherichia coli.
Weaver T, Banaszak L.
Biochemistry. 1996 Nov 5;35(44):13955-65.
PMID: 8909293
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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