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               PA0865               
Key to diagram


View in GBrowse: (?) 16 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name hpd  Alternate Gene Names
Locus tag PA0865 (View 6 updates) GenBank Identifier (GI) 15596062 (Precomputed BLAST results at BLINK )
Genomic location 945834 - 946907 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name 4-hydroxyphenylpyruvate dioxygenase (Class 2  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Cytoplasmic (Class 2Explanation of Localization Confidence)
Cytoplasmic (Class 3Explanation of Localization Confidence)
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Amino acid biosynthesis and metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0016763 transferase activity, transferring pentosyl groups IDA
GO:0003868 4-hydroxyphenylpyruvate dioxygenase activity IEA
Biological Process
GO:0006520 amino acid metabolic process ISS
GO:0006559 L-phenylalanine catabolic process IEA
GO:0006572 tyrosine catabolic process IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG3185 COG3185, 4-hydroxyphenylpyruvate dioxygenase and related hemolysins [Amino acid transport and metabolism / General function prediction only]. Amino acid transport and metabolism / General function prediction only
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
phenylalanine degradation I PseudoCyc PHENYLALANINE-DEG1-PWY
Ubiquinone and other terpenoid-quinone biosynthesis KEGG pae00130 Computationally Generated
Tyrosine metabolism KEGG pae00350 Computationally Generated
Phenylalanine metabolism KEGG pae00360 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 1.13.11.27
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 71% similar to 4-hydroxyphenylpyruvate dioxygenase [Pseudomonas sp]
76% similar to reported hemolysin [Vibrio vulnificus]
Most Similar Human Homolog
ENSP00000289004, Percent Identity: 32.54%, E-Value: 1e-29, Ensembl Release: 56.
Description: 4-hydroxyphenylpyruvate dioxygenase
Structure Features
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00903 Glyoxalase, Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily. 166 276 2e-11 23.0
 
Genomic Context
Genomic context Explanation of Genomic Context Field - Region contains several other genes involved in aromatic amino acid metabolism: adjacent gene is aroP (general aromatic amino acid transport), which is separated by three intervening genes from the phh locus (phenylalanine hydroxylase).
Microarray Expression Data
NCBI GEO Link
Sequence data
Upstream 500 BP Region GGCGGGACCGGCAGGGCCTCGTCGGGCGTCTCCAGCCACATCATCCCGGACACCGCATAGAGCACCTGGCCCCAGGCGTGCTCGTGGGCATCGACGCGGT GCCCGGCAGGATAGCTGCGGGCGACGCCGCGCAGGGGCCGGCGGCTGTCGCTCAGGTCGGGTGGTGCGGGTCGTGCCATGGGAATGCCGTCGCGGAGGAA AGACGCAGGTTAACCGCTTGACGCCGATCATGCAGCGCCGGCTTTCAGCGCAGCCGTGACAGCGCTGCTGGCAGCTTTTCGCACCGCACTGTAAAGATAA CTTTACGAAGACCGGGCGTTCGCCATGCATGCCGCCGGCGCGTAAGGAATAGTTGCCAGAAACCCTGCGCCAGGCCCTGTGCCGGGCCCTGCCGACGGCT TGTCCGCCGCGCCGCCACGGGGTGAGATGAAGGCATCTCCCATGTCGTCGGCCGCCTGGACCGGCGGCGGTCCATTCAACAAGAACAACGAGGAGGCCGG

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGAACGCCGTGGCCAAGATCGAACAGCACAATCCCATCGGTACCGACGGATTCGAATTCGTCGAGTTCACCGCCCCCGACGCCAAGGGCATCGAGCAGC TGCGCCAGCTGTTCAACATGATGGGCTTCACCGAAACCGCCAAGCATCGTTCCAAGGAAGTCTTCCTGTTCCAGCAGAACGATATCAACATCGTGCTCAA CGGCAGCCCAACCGGGCATGTCCATGAATTCGCCCTCAAGCACGGCCCGAGCGCCTGCGCCATGGCCTTCCGGGTGAAGAACGCTTCCCAGGCCGCCGCC TACGCCGAATCCCAGGGCGCCAAGCTGGTGGGCAGCCACGCCAACTTCGGCGAGCTGAACATCCCTTCCCTGGAAGGCATCGGCGGTTCGCTGCTGTATC TTGTCGACCGCTACGGCGACCGCAGCATCTATGACGTCGACTTCGAGTTCATCGAAGGCCGCAGCGCCAACGACAACTCGGTCGGCCTGACCTACATCGA CCACCTGACCCACAACGTCAAGCGCGGCCAGATGGACGTCTGGTCCGGTTTCTACGAGCGCATCGCCAACTTCCGCGAGATTCGCTACTTCGACATCGAA GGCAAGCTCACCGGCCTGTTCTCCCGCGCCATGACCGCACCTTGCGGGAAGATCCGCATCCCGATCAACGAGTCGGCCGACGATACCTCGCAGATCGAGG AATTCATCCGCGAATACCATGGCGAAGGCATCCAGCACATCGCCCTGACCACCGACGACATCTATGCCACCGTGCGCAAGCTGCGCGACAACGGCGTGAA GTTCATGTCGACCCCGGACACCTACTACGAGAAGGTCGACACCCGCGTCGCCGGGCATGGCGAGCCGCTCGAGCAACTGCGCGAACTGAACCTGCTGATC GACGGCGCCCCGGGCGACGACGGCATCCTGCTGCAGATCTTCACCGACACGGTGATCGGCCCGATCTTCTTCGAGATCATCCAGCGCAAGGGCAACCAGG GTTTCGGCGAGGGCAATTTCAAGGCCCTGTTCGAGTCCATCGAGGAAGACCAGATTCGCCGCGGCGTGATCTGA

BLAST search against this genome or other genomes in database


Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence MNAVAKIEQHNPIGTDGFEFVEFTAPDAKGIEQLRQLFNMMGFTETAKHRSKEVFLFQQNDINIVLNGSPTGHVHEFALKHGPSACAMAFRVKNASQAAA YAESQGAKLVGSHANFGELNIPSLEGIGGSLLYLVDRYGDRSIYDVDFEFIEGRSANDNSVGLTYIDHLTHNVKRGQMDVWSGFYERIANFREIRYFDIE GKLTGLFSRAMTAPCGKIRIPINESADDTSQIEEFIREYHGEGIQHIALTTDDIYATVRKLRDNGVKFMSTPDTYYEKVDTRVAGHGEPLEQLRELNLLI DGAPGDDGILLQIFTDTVIGPIFFEIIQRKGNQGFGEGNFKALFESIEEDQIRRGVI

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
References
PubMed Characterization of 4-hydroxyphenylpyruvate dioxygenase. Primary structure of the Pseudomonas enzyme.
Ruetschi U, Odelhog B, Lindstedt S, Barros-Soderling J, Persson B, Jornvall H.
Eur J Biochem. 1992 Apr 15;205(2):459-66.
PMID: 1572351
 
Alternate Annotations and Cross-References
TIGR NTL03PA00866
Systomonas PA0865
RefSeq NP_249556.1
GI 15596062
KEGG PA0865
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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