contact us| site map | view my clipboard
Add this annotation to your clipboard
               PA0871               
Key to diagram


View in GBrowse: (?) 16 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name phhB  Alternate Gene Names
Locus tag PA0871 (View 5 updates) GenBank Identifier (GI) 15596068 (Precomputed BLAST results at BLINK )
Genomic location 952514 - 952158 (-) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name pterin-4-alpha-carbinolamine dehydratase (Class 1  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Amino acid biosynthesis and metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0008124 4-alpha-hydroxytetrahydrobiopterin dehydratase activity RCA
Biological Process
GO:0006520 amino acid metabolic process IDA
GO:0006558 L-phenylalanine metabolic process IDA
GO:0006729 tetrahydrobiopterin biosynthetic process RCA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG2154 COG2154, Pterin-4a-carbinolamine dehydratase [Coenzyme metabolism]. Coenzyme metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Phenylalanine metabolism PseudoCAP
Reactions
EC Number 4.2.1.96
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 100% identical to pterin-4-alpha-carbinolamine dehydratase [Pseudomonas aeruginosa]
Most Similar Human Homolog
ENSP00000299299, Percent Identity: 33.33%, E-Value: 4e-05, Ensembl Release: 56.
Description: Pterin-4-alpha-carbinolamine dehydratase
Structure Features
Structure Features Explanation of Structure Features Field PF01329: Pterin 4 alpha carbinolamine dhydratase
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF01329 Pterin_4a, Pterin 4 alpha carbinolamine dehydratase. 13 112 9e-39 23.0
 
Genomic Context
Genomic context Explanation of Genomic Context Field - The third gene of a 4-gene cluster encoding phenylalanine hydroxylase (PhhA), carbinolamine dehydratase (PhhB), and aromatic aminotransferase (PhhC)
- and in the flanking upstream region, a divergently transcribed gene (phhR) encoding an activator protein.
Operons/Regulons
Type operon
Genes
GeneProduct
phhC aromatic amino acid aminotransferase
phhB pterin-4-alpha-carbinolamine dehydratase
phhA phenylalanine-4-hydroxylase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13293
Microarray Expression Data
NCBI GEO Link
Sequence data
Upstream 500 BP Region AACCCCTGGTTCGCCGAGTTCACCCATACCTACGGCAAGCTCGGCCTCAAGGCGAGCAAGGAGGAACGCGTGTTCCTCGCCCGCCTGTACTGGATGACCA TCGAGTTCGGCCTGGTCGAGACCGACCAGGGCAAGCGCATCTACGGCGGCGGCATCCTCTCCTCGCCGAAGGAGACCGTCTACAGCCTCTCCGACGAGCC GCTGCACCAGGCCTTCAATCCGCTGGAGGCGATGCGCACGCCCTACCGCATCGACATCCTGCAACCGCTCTATTTCGTCCTGCCCGACCTCAAGCGCCTG TTCCAACTGGCCCAGGAAGACATCATGGCGCTGGTCCACGAGGCCATGCGCCTGGGCCTGCACGCGCCGCTGTTCCCGCCCAAGCAGGCGGCCTGACGAG AAACGCCGCCGCCGCGGCTGACCCCGCGGCGGCCTTGTACTATGGTGGACGGGCACGGCCCGTCCGCCGCTCCCACATGCGACAACCAAGGACTCCCACC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGACCGCACTCACCCAAGCCCATTGCGAAGCCTGCCGCGCAGACGCCCCGCACGTCAGCGACGAAGAACTGCCCGTGCTGCTGCGGCAAATCCCGGATT GGAACATCGAAGTCCGCGACGGCATCATGCAGCTAGAGAAGGTCTACCTGTTCAAGAACTTCAAGCATGCCCTGGCCTTCACCAATGCCGTCGGCGAGAT ATCCGAGGCCGAAGGCCACCATCCGGGCCTGCTGACCGAGTGGGGCAAAGTCACCGTGACCTGGTGGAGCCACTCGATCAAGGGCCTGCACCGCAACGAT TTCATCATGGCGGCGCGCACCGATGAGGTAGCGAAAACCGCCGAGGGGCGCAAATGA

BLAST search against this genome or other genomes in database


Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence MTALTQAHCEACRADAPHVSDEELPVLLRQIPDWNIEVRDGIMQLEKVYLFKNFKHALAFTNAVGEISEAEGHHPGLLTEWGKVTVTWWSHSIKGLHRND FIMAARTDEVAKTAEGRK

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
References
PubMed Pseudomonas aeruginosa possesses homologues of mammalian phenylalanine hydroxylase and 4 alpha-carbinolamine dehydratase/DCoH as part of a three-component gene cluster.
Zhao G, Xia T, Song J, Jensen RA.
Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1366-70.
PMID: 8108417
 
Alternate Annotations and Cross-References
TIGR NTL03PA00872
Systomonas PA0871
RefSeq NP_249562.1
GI 15596068
KEGG PA0871
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

| What's New | FAQs | Cystic Fibrosis | Pseudomonas aeruginosa | Who is Involved? | Annotation Approach | Get Involved | Supplemental Data | Links to More Analyses | Site Map | Acknowledgements | Contact Us |