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               PA0872               
Key to diagram


View in GBrowse: 16 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name phhA  Alternate Gene Names
Locus tag PA0872 (View 6 updates) Submit an Update GenBank Identifier (GI) 15596069 (Precomputed BLAST results at BLINK )
Genomic location 953407 - 952619 (-) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name phenylalanine-4-hydroxylase (Class 1  Explanation of Product Name Confidence)
Alternate Product Names phenylalanine 4-monooxygenase
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA00873
Systomonas PA0872
RefSeq NP_249563.1
GI 15596069
KEGG PA0872
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
phhC aromatic amino acid aminotransferase
phhB pterin-4-alpha-carbinolamine dehydratase
phhA phenylalanine-4-hydroxylase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13293
Genomic Context
Genomic context Explanation of Genomic Context Field - and in the flanking upstream region, a divergently transcribed gene (phhR) encoding an activator protein.
- The second gene of a 4-gene cluster encoding phenylalanine hydroxylase (PhhA), carbinolamine dehydratase (PhhB), and aromatic aminotransferase (PhhC)
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Amino acid biosynthesis and metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0016775 phosphotransferase activity, nitrogenous group as acceptor IDA
GO:0004505 phenylalanine 4-monooxygenase activity IEA
Biological Process
GO:0006520 amino acid metabolic process IDA
GO:0009094 L-phenylalanine biosynthetic process IDA
GO:0006571 tyrosine biosynthetic process IDA
GO:0000162 tryptophan biosynthetic process IDA
GO:0006559 L-phenylalanine catabolic process IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG3186 COG3186, Phenylalanine-4-hydroxylase [Amino acid transport and metabolism]. Amino acid transport and metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Phenylalanine, tyrosine and tryptophan biosynthesis PseudoCAP
tyrosine biosynthesis II PseudoCyc TYRSYN2
phenylalanine degradation I PseudoCyc PHENYLALANINE-DEG1-PWY
Phenylalanine metabolism KEGG pae00360 Computationally Generated
Phenylalanine, tyrosine and tryptophan biosynthesis KEGG pae00400 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 1.14.16.1
Enzyme Assays
PDF file(s) provided by Sigma-Aldrich:
 L-Phenylalanine Hydroxylase (1.14.16.1)
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 99% identical to phenylalanine-4-hydroxylase [Pseudomonas aeruginosa]
Most Similar Human Homolog
ENSP00000370571, Percent Identity: 34.91%, E-Value: 6e-30, Ensembl Release: 56.
Description: Tyrosine 3-monooxygenase
Structure Features
Structure Features Explanation of Structure Features Field PS00367: Biopterin-dependent aromatic amino acid hydroxylases signature
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00351 Biopterin_H, Biopterin-dependent aromatic amino acid hydroxylase. 16 224 3e-37 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region CGACGCGCTTGACCCCGAAGACCCCCGGCACCGCCTCCAGCTTGGGCCGCAACGACTGCAACTGGAGGTTGATCATGTTCGGACAGAGCAGGTAGATGGC GTTGCCCTGGTCGCCGCCGACTTCGCCGCGATTGACGTTGATGCCGTAGTCGACCAGCAGGTTGAGGATGTCGCGGAGGATGCCTACACGGTTCTGGCAG TGCACTTTGATACGCATGGCGTGGCCCGGTGTTGTCGTTCTGATGGGCTCGCGGCCGGTTTTTTAACTCCCGGCCGGAAATTTCCGTCAAGAATATGTGA CAGTTAGACTCACCAGCAACAATGCCTGACCCAATGAACAGGCGACCCAGGCCAATTCGTAAGGAAAACTTTACGAAAACGCCGATTCCTCCCCGTTGCC CGGTCCCTATCCGGGGCTGCACGGACCGTCCCGACGCGGTATCGATAAGGCAACGACAACAACAAAGACCCCGCGGCCACAAGCCGCTCATGGAGTCCGT

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGAAAACGACGCAGTACGTGGCCCGCCAGCCCGACGACAACGGTTTCATCCACTATCCGGAAACCGAGCACCAGGTCTGGAATACCCTGATCACCCGGC AACTGAAGGTGATCGAAGGCCGCGCCTGTCAGGAATACCTCGACGGCATCGAACAGCTCGGCCTGCCCCACGAGCGGATCCCCCAGCTCGACGAGATCAA CAGGGTTCTCCAGGCCACCACCGGCTGGCGCGTGGCGCGGGTTCCGGCGCTGATTCCGTTCCAGACCTTCTTCGAACTGCTGGCCAGCCAGCAATTCCCC GTCGCCACCTTCATCCGCACCCCGGAAGAACTGGACTACCTGCAGGAGCCGGACATCTTCCACGAGATCTTCGGCCACTGCCCACTGCTGACCAACCCCT GGTTCGCCGAGTTCACCCATACCTACGGCAAGCTCGGCCTCAAGGCGAGCAAGGAGGAACGCGTGTTCCTCGCCCGCCTGTACTGGATGACCATCGAGTT CGGCCTGGTCGAGACCGACCAGGGCAAGCGCATCTACGGCGGCGGCATCCTCTCCTCGCCGAAGGAGACCGTCTACAGCCTCTCCGACGAGCCGCTGCAC CAGGCCTTCAATCCGCTGGAGGCGATGCGCACGCCCTACCGCATCGACATCCTGCAACCGCTCTATTTCGTCCTGCCCGACCTCAAGCGCCTGTTCCAAC TGGCCCAGGAAGACATCATGGCGCTGGTCCACGAGGCCATGCGCCTGGGCCTGCACGCGCCGCTGTTCCCGCCCAAGCAGGCGGCCTGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MKTTQYVARQPDDNGFIHYPETEHQVWNTLITRQLKVIEGRACQEYLDGIEQLGLPHERIPQLDEINRVLQATTGWRVARVPALIPFQTFFELLASQQFP VATFIRTPEELDYLQEPDIFHEIFGHCPLLTNPWFAEFTHTYGKLGLKASKEERVFLARLYWMTIEFGLVETDQGKRIYGGGILSSPKETVYSLSDEPLH QAFNPLEAMRTPYRIDILQPLYFVLPDLKRLFQLAQEDIMALVHEAMRLGLHAPLFPPKQAA

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0872 gene of Pseudomonas aeruginosa PAO1, Chromosome (952619 to 953407 bp)


Displaying the first five SNPs out of a total of 25 found in all isolate strains ( View all 25 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA7 Transition g.952622G>A
Silent
GCC(Ala) -> GCT(Ala) 		SNP-259671 View
Pseudomonas aeruginosa PA7 Transition g.952632T>C p.Lys259Arg
 Missense
AAG(Lys) -> AGG(Arg) 		SNP-259672 View
Pseudomonas aeruginosa PA14 Transition g.952685C>T
Silent
GCG(Ala) -> GCA(Ala) 		SNP-181320 View
Pseudomonas aeruginosa PA7 Transition g.952709T>C
Silent
CAA(Gln) -> CAG(Gln) 		SNP-259673 View
Pseudomonas aeruginosa PA7 Transition g.952742A>G
Silent
TAT(Tyr) -> TAC(Tyr) 		SNP-259674 View
Select to view SNPs in a specific strain
SNP Map
PubMed Pseudomonas aeruginosa possesses homologues of mammalian phenylalanine hydroxylase and 4 alpha-carbinolamine dehydratase/DCoH as part of a three-component gene cluster.
Zhao G, Xia T, Song J, Jensen RA.
Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1366-70.
PMID: 8108417
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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