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               PA0887               
Key to diagram


View in GBrowse: (?) 22 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name acsA  Alternate Gene Names
Locus tag PA0887 (View 6 updates) GenBank Identifier (GI) 15596084 (Precomputed BLAST results at BLINK )
Genomic location 969670 - 971625 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
POG000854 
Possible inparalog involved.
Orthology uncertain because more than one top BLASTp hit was identified.
Product Information
Product Name acetyl-coenzyme A synthetase (Class 2  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Carbon compound catabolism
Central intermediary metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0004823 leucine-tRNA ligase activity ISS
GO:0003987 acetate-CoA ligase activity RCA
GO:0003987 acetate-CoA ligase activity IEA
Biological Process
GO:0044248 cellular catabolic process ISS
GO:0015978 carbon utilization by utilization of organic compounds ISS
GO:0008152 metabolic process ISS
GO:0006090 pyruvate metabolic process ISS
GO:0019541 propionate metabolic process ISS
GO:0019643 reductive tricarboxylic acid cycle ISS
GO:0019427 acetyl-CoA biosynthetic process from acetate RCA
GO:0019427 acetyl-CoA biosynthetic process from acetate IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0365 Acs, Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases [Lipid metabolism]. Lipid metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Reductive carboxylate cycle (CO2 fixation) PseudoCAP
Pyruvate metabolism PseudoCAP
Propanoate metabolism PseudoCAP
acetate degradation PseudoCyc ACETATEUTIL-PWY
Glycolysis / Gluconeogenesis KEGG pae00010 Computationally Generated
Pyruvate metabolism KEGG pae00620 Computationally Generated
Propanoate metabolism KEGG pae00640 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 6.2.1.1
Enzyme Assays
PDF file(s) provided by Sigma-Aldrich:
 S-Acetyl Coenzyme A Synthetase (6.2.1.1)
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 80% similar to acetyl-CoA sythetase [Escherichia coli]
Most Similar Human Homolog
ENSP00000353804, Percent Identity: 53.98%, E-Value: 0.0, Ensembl Release: 56.
Description: Acetyl-coenzyme A synthetase, cytoplasmic
Structure Features
Structure Features Explanation of Structure Features Field PF00501: AMP-binding enzyme
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00501 AMP-binding, AMP-binding enzyme. 109 547 1e-102 23.0
 
Microarray Expression Data
NCBI GEO Link
Sequence data
Upstream 500 BP Region GCTGCTGGTCGCCGGCAGCTTCATGGAGCCCTCGGCGATCGTGCTGATCCTGGCGCCGATCTTCTTCCCCATCGCCATGAAGCTGGGCATCGACCCGATC CACCTGGGCATCGTCATGGTGGTGAACATGGAGATCGGCCTGGTACACCCGCCGGTCGGCCTCAACCTGTTCGTCACTTCGGCGGTCACCGGCATGCCGC TGGGGGCGACCATCCGCGCGGCGCTGCCGTGGCTGATGATCCTGCTGCTGTTCCTGATCCTGGTGACCTACGTGCCGTTGATCTCGCTGTGGCTGCCGGG CCTGCTGGGCATGAGCTGAGGTCGCGGCGGCGGGGCGCGGGACGCGCTCCGCCGCTGGCGCAAGGCTACGATCGGCGCGGCTTATAGCTACTATCGGCGC TGTCGCGCAGTGGTAAGACCATGGTCGTATGGTTCGTGGGGTGTGCGCTCGCTACAACTGGGCACAACAAAAACAACACTCACGTACCGAGGTTAAAGCC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGTCTGCGGCATCCCTGTACCCCGTGCACCCGGAAGCGGTGGCACGGACCTTCACCGACGAAGCCACCTACAAGACGATGTACCAGCAGTCCGTCGTCA ACCCCGACGGCTTCTGGCGCGAGCAGGCCCAGCGGATCGACTGGATCAAGCCGTTCGAGAAGGTCAAGCAGACCTCCTTCGACGACCATCACGTGGACAT CAAGTGGTTCGCCGACGGCACCCTCAACGTGTCGCACAACTGCCTCGACCGTCACCTCGCCGAACGCGGCGACCAGGTGGCGATCATCTGGGAAGGCGAC GATCCCGCCGACCACCAGGAAATCACCTACCGCCAGCTCCACGAGCAGGTCTGCAAGTTCGCCAACGCCCTGCGCGGGCAGGACGTGCACCGCGGCGACG TAGTGACCATCTACATGCCGATGATCCCCGAGGCCGTGGTCGCCATGCTCGCCTGCACCCGCATCGGCGCGATCCACTCGGTGGTCTTCGGCGGCTTCTC CCCCGAGGCCCTGGCAGGACGCATCATCGATTGCAAGTCGAAGGTGGTGATCACCGCCGACGAAGGCGTGCGCGGCGGCAAGCGCACTCCGCTGAAGGCC AATGTCGACGACGCCCTGACCAACCCGGAAACCTCCAGCGTGCAGAAGATCATCGTCTGCAAGCGTACCGGTGCGGAGATCAAGTGGAACCAGCACCGCG ACGTCTGGTACGACGACCTGATGAAGGTTGCCGGCAGCACTTGCGCACCCAAGGAAATGGGCGCCGAGGACCCGCTGTTCATCCTCTACACCTCCGGCTC CACCGGCAAGCCGAAGGGCGTGCTGCATACCACCGGCGGCTACCTGGTGTACGCCTCGCTGACCCACGAGCGGGTCTTCGACTACCGTCCGGGCGAAGTC TACTGGTGCACCGCCGACATCGGCTGGGTCACCGGCCACACCTACATCGTCTATGGCCCGTTGGCCAACGGCGCCACCACCATTCTGTTCGAGGGCGTGC CGAACTACCCCGACGTGACCCGCGTGGCGAAAATCATCGACAAGCACAAGGTCAACATCCTCTACACCGCGCCGACCGCGATCCGCGCGATGATGGCCGA AGGCAAGGCGGCGGTGGCCGGTGCCGACGGTTCCAGCCTGCGTCTGCTCGGTTCGGTGGGCGAGCCGATCAACCCGGAAGCCTGGCAGTGGTACTACGAG ACCGTCGGCCAGTCGCGCTGCCCGATCGTCGACACCTGGTGGCAGACCGAGACCGGCGCCTGCCTGATGACCCCGCTGCCGGGCGCCCACGCGATGAAGC CGGGCTCTGCAGCCAAGCCGTTCTTCGGCGTGGTACCGGCGCTGGTGGACAACCTCGGCAACCTGATCGAGGGCGCCGCCGAGGGCAACCTGGTGATCCT CGACTCCTGGCCGGGCCAGGCGCGGACCCTGTTCGGCGACCATGACCGCTTCGTCGACACCTACTTCAAGACCTTCAAGGGCATGTACTTCACCGGCGAC GGCGCGCGCCGCGACGAGGACGGCTACTACTGGATCACCGGGCGGGTCGACGACGTGCTCAACGTCTCCGGCCACCGCATGGGCACCGCCGAGGTGGAAA GCGCGATGGTCGCCCACCCGAAGGTCGCCGAGGCGGCGGTGGTCGGCATGCAGCACGACATCAAGGGGCAGGGCATCTATGTCTACGTCACCCTCAACTC CGGGGTCGAGCCGAGCGAGGCGCTGCGCCAGGAGCTGAAGCAATGGGTTCGCCGCGAGATCGGTCCGATCGCCACGCCGGATGTGATCCAGTGGGCGCCG GGACTGCCGAAGACCCGTTCGGGCAAGATCATGCGGCGCATCCTGCGCAAGATCGCCGCGGCCGAGTACGACACCCTCGGCGACATCTCCACCCTTGCCG ACCCGGGCGTGGTCCAGCACCTGATCGAGACCCATCGCTCGATGCAGGCCGCCTGA

BLAST search against this genome or other genomes in database


Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence MSAASLYPVHPEAVARTFTDEATYKTMYQQSVVNPDGFWREQAQRIDWIKPFEKVKQTSFDDHHVDIKWFADGTLNVSHNCLDRHLAERGDQVAIIWEGD DPADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADEGVRGGKRTPLKA NVDDALTNPETSSVQKIIVCKRTGAEIKWNQHRDVWYDDLMKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEV YWCTADIGWVTGHTYIVYGPLANGATTILFEGVPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYE TVGQSRCPIVDTWWQTETGACLMTPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDSWPGQARTLFGDHDRFVDTYFKTFKGMYFTGD GARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEALRQELKQWVRREIGPIATPDVIQWAP GLPKTRSGKIMRRILRKIAAAEYDTLGDISTLADPGVVQHLIETHRSMQAA

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
References
PubMed Cloning, characterization, and functional expression of acs, the gene which encodes acetyl coenzyme A synthetase in Escherichia coli.
Kumari S, Tishel R, Eisenbach M, Wolfe AJ.
J Bacteriol. 1995 May;177(10):2878-86.
PMID: 7751300
 
Alternate Annotations and Cross-References
TIGR NTL03PA00888
Systomonas PA0887
RefSeq NP_249578.1
GI 15596084
KEGG PA0887
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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