contact us| site map | view my clipboard
Add this annotation to your clipboard
               PA0932               
Key to diagram


View in GBrowse: (?) 16 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name cysM  Alternate Gene Names
Locus tag PA0932 (View 6 updates) GenBank Identifier (GI) 15596129 (Precomputed BLAST results at BLINK )
Genomic location 1020708 - 1021607 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name cysteine synthase B (Class 2  Explanation of Product Name Confidence)
Alternate Product Names O-acetylserine sulfhydrylase B
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Amino acid biosynthesis and metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0004333 fumarate hydratase activity ISS
GO:0004124 cysteine synthase activity IEA
Biological Process
GO:0006520 amino acid metabolic process ISS
GO:0006790 sulfur metabolic process ISS
GO:0006535 cysteine biosynthetic process from serine IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0031 CysK, Cysteine synthase [Amino acid transport and metabolism]. Amino acid transport and metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Sulfur metabolism PseudoCAP
Selenoamino acid metabolism PseudoCAP
Cysteine metabolism PseudoCAP
cysteine biosynthesis I PseudoCyc CYSTSYN-PWY
Cysteine and methionine metabolism KEGG pae00270 Computationally Generated
Selenoamino acid metabolism KEGG pae00450 Computationally Generated
Sulfur metabolism KEGG pae00920 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 4.2.99.8
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 83% similar to E. coli cysteine synthase B.
Most Similar Human Homolog
ENSP00000381234, Percent Identity: 36.86%, E-Value: 2e-47, Ensembl Release: 56.
Description: Cystathionine beta-synthase
Structure Features
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00291 PALP, Pyridoxal-phosphate dependent enzyme. 8 285 5e-67 23.0
 
Operons/Regulons
Type operon
Genes
GeneProduct
cysM cysteine synthase B
ygcA probable RNA methyltransferase
relA GTP pyrophosphokinase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13304
Microarray Expression Data
NCBI GEO Link
Sequence data
Upstream 500 BP Region GCCGCCAGGAGCCGCCGAAGCATGGCACCAGCCGCAACACGCCGGTGGTCTCGCGAAAAGAGGTGGGTACCTATGGCATCTGGGGCGTCAGCGCCGGCTA CACCTTCAGCGAGAACCTGAGCGTACGGGGCGGGGTAAGCAACCTCTTCGACAAGCGCCTGTACCGCCAGGGCAACTCCTTCGACGCCGGCGCGGCAACC TACAACGAGCCGGGTCGCGCCTACTACGTTTCGATGACCACCTCGTTCTGATCGCCGCGGCCAACGCGCTTTCACCGGCAGGCGCAACGCCTGTCGGACC TTTCTTTGGCGTTGGTCAGGCATCGGCTGTCCAACGCCAGGTTTTCTCTTCGGACGCCTCTGGTCATCGCATCGCATGCCGTTTCGGGCGGTCTTTCGGC TGAATCGGCATGCATGACCGGATATTGCGGTGCTAGGGTCCGGCCCCAGTTTCCCGGTATCATGCTCAGCCTTTACCGTCAGTGCCGAACGAGTGCCTCC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGACCGTGCAGTACCCGACTATCGCCGACTGCGTTGGCAATACCCCTCTGGTGCGTCTGCAGCGCCTTCCCGGGGAAACCTCCAATACCCTGCTGGTGA AGCTCGAAGGCAACAACCCGGCGGGATCGGTGAAGGACCGCCCGGCCCTGTCGATGATCACCCGTGCCGAACTGCGCGGCGACATCCGCCCCGGCGATAC GCTGATCGAGGCTACTTCCGGCAACACCGGCATCGCCCTGGCCATGGCGGCGGCGATCAAGGGTTACAAGATGATCCTGATCATGCCGGACAACTCCACG GCCGAACGCAAGGCGGCGATGACCGCCTATGGCGCCGAGCTCATTCTGGTCAGCAAGGAAGAGGGCATGGAGGGCGCGCGCGATCTGGCCGACAAGCTGC AGCGCGAAGGCCGCGGCAAGGTCCTCGACCAGTTCGCCAATGGCGACAACCCGGAAGCCCATTACCACAGCACCGGCCCGGAAATCTGGCAGCAGACCGG CGGCAGCATCACCCATTTCGTCAGCTCCATGGGCACCACCGGGACCATCATGGGCGTCTCGCGCTACCTCAAGGAGCAGAACCCGGCGGTGCAGATCGTC GGCCTGCAACCCATGGAGGGCTCGGCCATCCCGGGAATCCGACGCTGGCCGCAGGAATACCTGCCGAAGATCTATGACGCCAGTCGCGTCGACCGGGTCG TCGACATGCACCAGGACGAAGCCGAGGACATCATGCGCCGGCTGGCCCGCGAGGAAGGCATCTTCTGCGGCGTGTCCTCCGGTGGGGCGGTGGCGGCGAT GCTGCGCCTGTCCCGCGAACTGGAGAATGCCGTGCTGGTGGCGATCATCTGCGACCGCGGCGACCGCTACCTGTCTTCCGGCGTCTATGACCCGCGCTGA

BLAST search against this genome or other genomes in database


Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence MTVQYPTIADCVGNTPLVRLQRLPGETSNTLLVKLEGNNPAGSVKDRPALSMITRAELRGDIRPGDTLIEATSGNTGIALAMAAAIKGYKMILIMPDNST AERKAAMTAYGAELILVSKEEGMEGARDLADKLQREGRGKVLDQFANGDNPEAHYHSTGPEIWQQTGGSITHFVSSMGTTGTIMGVSRYLKEQNPAVQIV GLQPMEGSAIPGIRRWPQEYLPKIYDASRVDRVVDMHQDEAEDIMRRLAREEGIFCGVSSGGAVAAMLRLSRELENAVLVAIICDRGDRYLSSGVYDPR

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
References
PubMed Sulfate and thiosulfate transport in Escherichia coli K-12: nucleotide sequence and expression of the cysTWAM gene cluster.
Sirko A, Hryniewicz M, Hulanicka D, Bock A.
J Bacteriol. 1990 Jun;172(6):3351-7.
PMID: 2188958
 
Alternate Annotations and Cross-References
TIGR NTL03PA00933
Systomonas PA0932
RefSeq NP_249623.1
GI 15596129
KEGG PA0932
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

| What's New | FAQs | Cystic Fibrosis | Pseudomonas aeruginosa | Who is Involved? | Annotation Approach | Get Involved | Supplemental Data | Links to More Analyses | Site Map | Acknowledgements | Contact Us |