Pseudomonas Genome Database - Annotation for PA0944

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PA0944

Key to diagram

View in GBrowse:

16 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Operons/Regulons
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name	purN	Alternate Gene Names
Locus tag	PA0944 (View 5 updates) Submit an Update	GenBank Identifier (GI)	15596141 (Precomputed BLAST results at BLINK )
Genomic location	1032763 - 1032095 (-)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG000910
Product Information
Product Name	phosphoribosylaminoimidazole synthetase (Class 2 )
Alternate Product Names	phosphoribosylformylglycinamidine cyclo-ligase
Subcellular localization	Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA00945
Systomonas	PA0944
RefSeq	NP_249635.1
GI	15596141
KEGG	PA0944
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Operons/Regulons

Type

operon

Genes

Gene	Product
PA0943	hypothetical protein
purN	phosphoribosylaminoimidazole synthetase
purM	phosphoribosylaminoimidazole synthetase

Evidence

Computationally Predicted

PubMed

18988623

Cross-Reference

DOOR-13306

Genomic Context

Genomic context

- PurM and PurN are encoded by adjacent genes, as in E. coli.

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Nucleotide biosynthesis and metabolism

GO Accession

GO Term

Evidence

Molecular Function

GO:0000104	succinate dehydrogenase activity	ISS
GO:0004644	phosphoribosylglycinamide formyltransferase activity	IEA

Biological Process

GO:0009117	nucleotide metabolic process	ISS
GO:0009152	purine ribonucleotide biosynthetic process	IEA

Cellular Component

COG Prediction

Accession	Description	Category
COG0299	PurN, Folate-dependent phosphoribosylglycinamide formyltransferase PurN [Nucleotide transport and metabolism].	Nucleotide transport and metabolism

Pathways

Name	Database	Xref
One carbon pool by folate	PseudoCAP
Purine metabolism	PseudoCAP
de novo biosynthesis of purine nucleotides	PseudoCyc	DENOVOPURINE2-PWY
his+purine+pyrimidine biosynthesis	PseudoCyc	PRPP-PWY
Purine metabolism	KEGG	pae00230	Computationally Generated
One carbon pool by folate	KEGG	pae00670	Computationally Generated
Metabolic pathways	KEGG	pae01100	Computationally Generated

Reactions

EC Number

2.1.2.2

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

73% similar to E. coli purN gene product.

Most Similar Human Homolog

ENSP00000371253, Percent Identity: 48.74%, E-Value: 6e-49, Ensembl Release: 56.
Description: Trifunctional purine biosynthetic protein adenosine-3

Structure Features

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF00551	Formyl_trans_N, Formyl transferase.	6	184	1e-59	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

TCCGGCCCGCACTCCAACGGCTACTCCCTGATCCGCAAGATCATCGAGGTTTCCGGCGCCGACATCGCGCAGGTCCAACTCGACGGCAAGCCGCTGGCCG ACCTGCTGATGGCGCCGACCCGCATCTACGTCAAGCCGCTGCTGCAACTGATCAAGCAGACCGGCGCGGTCAAGGCCATGGCTCACATTACCGGCGGCGG CCTGCTGGACAACATCCCGCGCGTCCTGCCGGACAACGCCCAGGCCGTGATCGATGTCGCCAGCTGGAACCGTCCGGCGGTATTCGACTGGCTGCAGGAA CAGGGCAACGTCGACGAGACCGAGATGCATCGCGTACTCAACTGCGGCGTCGGCATGGTCATCTGCGTGGCCCAGAGCGACGCCGAGAAAGCCCTGGAAG TCCTGCGTGCCGCCGGCGAGCAACCCTGGCAGATCGGTCGCATCGAAACCTGCGGCGCGGACGCCGAGCGCGTGGTCCTGAACAATCTGAAAAACCACTG

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGCCGAAACTCTGCAATGTCGTGGTGCTGATTTCCGGCTCCGGCAGCAACCTGCAGGCGCTGATCGACAGCCTCCGGGACGGCGCCACGCCGGCCCGGA TCCGCGCGGTGATCTCCAACCGCGCGGACGCCTATGGCCTGGAACGCGCACGCCAGGCCGGCATCCAGACCGAGGTGCTCGATCACAAGGCCTATGCCGA TCGCGAAAGCTTCGACGAGGCCCTGGCACAACGGATCGATGCCCATGAACCGCACCTGGTGATCCTTGCCGGGTTCATGCGCATCCTCAGCGCCGATTTC GTTCGCCATTACCAGGGGCGTCTGCTGAATATCCATCCCTCGTTGCTGCCGCGCCACAAAGGGCTGCATACCCATCAACGCGCCCTCGAAGCCGGCGACC GCGAGCACGGTTGCAGCGTGCACTTCGTGACGGAGGAACTCGATGGGGGGCCACTGGTCGTACAGGCAGTAATCCCGGTAGAGTCGCAAGATACGCCGGA ACGCCTGGCCAGACGGGTTCACGAGGAAGAACACCGGATCTACCCGCTGGCCATGCGCTGGTTCGCCGAGGGCCGCTTGCGCCTGGGCGAACAGGGCGCC CTGCTGGATGGCCAGGCCCTGCCTGCCACCGGCCATCAGATTCGCACCAAGGAGATTCGCAATGCGTAA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MPKLCNVVVLISGSGSNLQALIDSLRDGATPARIRAVISNRADAYGLERARQAGIQTEVLDHKAYADRESFDEALAQRIDAHEPHLVILAGFMRILSADF VRHYQGRLLNIHPSLLPRHKGLHTHQRALEAGDREHGCSVHFVTEELDGGPLVVQAVIPVESQDTPERLARRVHEEEHRIYPLAMRWFAEGRLRLGEQGA LLDGQALPATGHQIRTKEIRNA

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0944 gene of Pseudomonas aeruginosa PAO1, Chromosome (1032095 to 1032763 bp)

Displaying the first five SNPs out of a total of 60 found in all isolate strains ( View all 60 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa PA7	Transition	g.1032194C>T		Silent GAG(Glu) -> GAA(Glu)	SNP-240190	View
Pseudomonas aeruginosa PA7	Transition	g.1032221G>A		Silent TAC(Tyr) -> TAT(Tyr)	SNP-240191	View
Pseudomonas aeruginosa PA7	Transition	g.1032314C>T		Silent GGG(Gly) -> GGA(Gly)	SNP-240192	View
Pseudomonas aeruginosa PA7	Transition	g.1032320A>G		Silent GAT(Asp) -> GAC(Asp)	SNP-240193	View
Pseudomonas aeruginosa PA7	Transition	g.1032353A>G		Silent GGT(Gly) -> GGC(Gly)	SNP-240194	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Structures of apo and complexed Escherichia coli glycinamide ribonucleotide transformylase.
Almassy RJ, Janson CA, Kan CC, Hostomska Z.
Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6114-8.
PMID: 1631098