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               PA0945               
Key to diagram


View in GBrowse: 16 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name purM  Alternate Gene Names
Locus tag PA0945 (View 7 updates) Submit an Update GenBank Identifier (GI) 15596142 (Precomputed BLAST results at BLINK )
Genomic location 1033824 - 1032763 (-) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name phosphoribosylaminoimidazole synthetase (Class 2  Explanation of Product Name Confidence)
Alternate Product Names phosphoribosylformylglycinamidine cyclo-ligase
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA00946
Systomonas PA0945
RefSeq NP_249636.1
GI 15596142
KEGG PA0945
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Nucleotide biosynthesis and metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0008716 D-alanine-D-alanine ligase activity ISS
GO:0004641 phosphoribosylformylglycinamidine cyclo-ligase activity IEA
Biological Process
GO:0009117 nucleotide metabolic process ISS
GO:0006189 'de novo' IMP biosynthetic process IEA
GO:0009152 purine ribonucleotide biosynthetic process IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0150 PurM, Phosphoribosylaminoimidazole (AIR) synthetase [Nucleotide transport and metabolism]. Nucleotide transport and metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Purine metabolism PseudoCAP
de novo biosynthesis of purine nucleotides PseudoCyc DENOVOPURINE2-PWY
his+purine+pyrimidine biosynthesis PseudoCyc PRPP-PWY
Purine metabolism KEGG pae00230 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 6.3.3.1
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 82% similar to E. coli purM gene product.
Most Similar Human Homolog
ENSP00000371253, Percent Identity: 50.14%, E-Value: 2e-92, Ensembl Release: 56.
Description: Trifunctional purine biosynthetic protein adenosine-3
Structure Features
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00586 AIRS, AIR synthase related protein, N-terminal domain. 44 143 3e-21 23.0
PF02769 AIRS_C, AIR synthase related protein, C-terminal domain. 177 336 4e-27 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region AAATCGACCACCAGCGCCAGCGGCGGACCATTTTCGAAGGCGTACTGGCTGATCAGTTGCTGCGGATCCTTCAGGTACCCCGCCAATGCCGGGTTCTGCG GCGCCTGCGGATTGCCGGTCAGGCGCACCAGCAGGGTCTGCAGGGCTCGCACCAGTCCGGCATCGCGCTCCCCGGGCTGCTGCGACGAAACCGGCTCGTG GACCTGGTAGAGATTGGGGACCGTTGCCGCGAAGACGGGCAAGCCTAGCAGGGAAAAACAGAGAACGAAGAGTCGGGCTATGCGCATGGAGGGCTCTCGA CGAACGGATGGGAACGGCGGCGACGAGCTGTGAAGGCCGCGCAAGTGGACCTATACCTTAAACAGCAGTCCGTCCAGCGGCAACCGAGATGGCCGCTGCG TGGCAAGCCTGATAAAATCGCGCGCCTTCAAAACCGGCAAACAGCGTTGCACGCCTGGGCGGCCGGCCGATCTCCGAATTACCCCTATAGGCCTGGATTT

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGAGCAGCAAGCAACCCTCGTTGAGCTACAAGGACGCTGGTGTGGACATCGACGCCGGCGAAGCCCTGGTGGAACGCATCAAAGGCGTGGCCAAGCGCA CCGCACGCCCGGAAGTGATGGGCGGCCTCGGCGGCTTCGGCGCCCTCTGCGAAATCCCGGCCGGCTACAAGCAGCCGGTGCTGGTATCCGGCACCGACGG GGTGGGCACCAAGCTGCGCCTGGCGCTCAACCTGAACAAGCACGACAGCATCGGCCAGGACCTGGTGGCGATGTGCGTCAACGACCTGGTGGTCTGCGGC GCCGAGCCGCTGTTCTTTCTCGACTACTACGCCACCGGCAAGCTCAACGTCGACGTCGCCGCCACCGTAGTCACCGGCATCGGTGCCGGTTGCGAACTGG CGGGCTGTTCCCTGGTCGGCGGCGAGACCGCCGAAATGCCCGGCATGTACGAAGGCGAAGACTATGACCTGGCCGGCTTCTGCGTCGGCGTCGTGGAAAA GGCCGAGATCATCGACGGCTCCAGGGTCCAGGCCGGCGACGCGCTTATCGCCCTGCCCTCCTCCGGCCCGCACTCCAACGGCTACTCCCTGATCCGCAAG ATCATCGAGGTTTCCGGCGCCGACATCGCGCAGGTCCAACTCGACGGCAAGCCGCTGGCCGACCTGCTGATGGCGCCGACCCGCATCTACGTCAAGCCGC TGCTGCAACTGATCAAGCAGACCGGCGCGGTCAAGGCCATGGCTCACATTACCGGCGGCGGCCTGCTGGACAACATCCCGCGCGTCCTGCCGGACAACGC CCAGGCCGTGATCGATGTCGCCAGCTGGAACCGTCCGGCGGTATTCGACTGGCTGCAGGAACAGGGCAACGTCGACGAGACCGAGATGCATCGCGTACTC AACTGCGGCGTCGGCATGGTCATCTGCGTGGCCCAGAGCGACGCCGAGAAAGCCCTGGAAGTCCTGCGTGCCGCCGGCGAGCAACCCTGGCAGATCGGTC GCATCGAAACCTGCGGCGCGGACGCCGAGCGCGTGGTCCTGAACAATCTGAAAAACCACTGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MSSKQPSLSYKDAGVDIDAGEALVERIKGVAKRTARPEVMGGLGGFGALCEIPAGYKQPVLVSGTDGVGTKLRLALNLNKHDSIGQDLVAMCVNDLVVCG AEPLFFLDYYATGKLNVDVAATVVTGIGAGCELAGCSLVGGETAEMPGMYEGEDYDLAGFCVGVVEKAEIIDGSRVQAGDALIALPSSGPHSNGYSLIRK IIEVSGADIAQVQLDGKPLADLLMAPTRIYVKPLLQLIKQTGAVKAMAHITGGGLLDNIPRVLPDNAQAVIDVASWNRPAVFDWLQEQGNVDETEMHRVL NCGVGMVICVAQSDAEKALEVLRAAGEQPWQIGRIETCGADAERVVLNNLKNH

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA0945 gene of Pseudomonas aeruginosa PAO1, Chromosome (1032763 to 1033824 bp)


Displaying the first five SNPs out of a total of 72 found in all isolate strains ( View all 72 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA7 Transition g.1032770T>C p.Asn352Ser
 Missense
AAC(Asn) -> AGC(Ser) 		SNP-240240 View
Pseudomonas aeruginosa PA7 Transition g.1032778A>G
Silent
AAT(Asn) -> AAC(Asn) 		SNP-240241 View
Pseudomonas aeruginosa PACS2 Transition g.1032794C>T p.Arg344His
 Missense
CGC(Arg) -> CAC(His) 		SNP-129178 View
Pseudomonas aeruginosa PA7 Transition g.1032802G>A
Silent
GAC(Asp) -> GAT(Asp) 		SNP-240242 View
Pseudomonas aeruginosa PA7 Transition g.1032823G>A
Silent
CGC(Arg) -> CGT(Arg) 		SNP-240243 View
Select to view SNPs in a specific strain
SNP Map
Operons/Regulons
Type operon
Genes
GeneProduct
PA0943 hypothetical protein
purN phosphoribosylaminoimidazole synthetase
purM phosphoribosylaminoimidazole synthetase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13306
Genomic Context
Genomic context Explanation of Genomic Context Field - PurM and PurN are encoded by adjacent genes, as in E. coli.
PubMed Nucleotide sequence of the purM gene encoding 5'-phosphoribosyl-5-aminoimidazole synthetase of Escherichia coli K12.
Smith JM, Daum HA 3rd.
J Biol Chem. 1986 Aug 15;261(23):10632-6.
PMID: 3015935
Identification and nucleotide sequence of a gene encoding 5'-phosphoribosylglycinamide transformylase in Escherichia coli K12.
Smith JM, Daum HA 3rd.
J Biol Chem. 1987 Aug 5;262(22):10565-9.
PMID: 3301838
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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