Pseudomonas Genome Database - Annotation for PA1001

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PA1001

Key to diagram

View in GBrowse:

3 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Operons/Regulons
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name	phnA	Alternate Gene Names
Locus tag	PA1001 (View 7 updates) Submit an Update	GenBank Identifier (GI)	15596198 (Precomputed BLAST results at BLINK )
Genomic location	1083972 - 1085564 (+)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG000964
Product Information
Product Name	anthranilate synthase component I (Class 1 )
Alternate Product Names	phenazine biosynthesis protein PhnA
Subcellular localization	Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA01002
Systomonas	PA1001
RefSeq	NP_249692.1
GI	15596198
KEGG	PA1001
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Operons/Regulons

Type

operon

Genes

Gene	Product
phnA	anthranilate synthase component I
phnB	anthranilate synthase component II

Evidence

Computationally Predicted

PubMed

18988623

Cross-Reference

DOOR-13318

Genomic Context

Genomic context

- PhnB is downstream and has been reported to be cotranscribed.

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Adaptation, Protection

GO Accession

GO Term

Evidence

Molecular Function

GO:0004049

anthranilate synthase activity

IEA

Biological Process

GO:0050896	response to stimulus	IDA
GO:0000162	tryptophan biosynthetic process	IEA

Cellular Component

COG Prediction

Accession	Description	Category
COG0147	TrpE, Anthranilate/para-aminobenzoate synthases component I [Amino acid transport and metabolism / Coenzyme metabolism].	Amino acid transport and metabolism / Coenzyme metabolism

Pathways

Name	Database	Xref
Ubiquinone biosynthesis	PseudoCAP
Phenylalanine, tyrosine and tryptophan biosynthesis	PseudoCAP
phenylalanine, tyrosine and tryptophan biosynthesis, complete	PseudoCyc	COMPLETE-ARO-PWY
tryptophan biosynthesis	PseudoCyc	TRPSYN-PWY
pathways of chorismate	PseudoCyc	ALL-CHORISMATE-PWY
Phenylalanine, tyrosine and tryptophan biosynthesis	KEGG	pae00400	Computationally Generated
Metabolic pathways	KEGG	pae01100	Computationally Generated
Two-component system	KEGG	pae02020	Computationally Generated

Reactions

EC Number

4.1.3.27

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

64% similar to anthranilate synthase component I TrpE [Escherichia coli]
100% similar to phenazine biosynthesis protein PhnA [Pseudomonas aeruginosa]

Structure Features

TETRAMER OF TWO COMPONENTS I AND TWO COMPONENTS II. COMPONENT I CATALYZES THE FORMATION OF ANTHRANILATE USING AMMONIA RATHER THAN GLUTAMINE, WHEREAS COMPONENT II PROVIDES LUTAMINE AMIDOTRANSFERASE ACTIVITY

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF04715	Anth_synt_I_N, Anthranilate synthase component I, N terminal region.	99	195	1e-14	23.0
PF00425	Chorismate_bind, chorismate binding enzyme.	244	513	1e-109	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

TCTGCGGCGATGCCCTGGGCGAGTTCGACGAGGCAGAGGGGGTGTGGCGGCCGCTGGTGTTCGACGACATGGAGGCTTACCTGGAGTCCCTGGAACGTCT GCAGCGTCTGCCGACCCTGCTGCAACTGATCCCGGGACACGGCGGCCTGCTGCGGGGGCGGCTGGCCGCGGATGGGGCCGAGTCGGCCTATACCGAGTGT CTGCGCCTGTGCCGGCGGTTGCTCTGGCGCCAGTCCATGGGCGAATCCCTCGACGAACTGAGCGAGGAGCTGCACCGCGCCTGGGGTGGGCAGAGCGTCG ACTTCCTGCCCGGCGAACTGCACCTGGGGAGCATGCGCCGGATGCTGGAGATTCTCTCCCGCCAGGCGCTGCCTCTGGACTGAGACGGGACATCCATTGC GGCGATCGCCGCCGACGGCAGCGGTCGCAATTGGGGGAAAAGGGGGGTTACCGATGATGAACATGCCGTTGCGCGCTAGCGTCGCGCAGGCCAGTCGCCC

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGGGCGCGAGGAGGTGGCTCGTGAGCGGGGTTGGCTATCGACTGGAAGAAAGTCTGGAGTACCGCACGCTGGTGCCGGAGGCGCTGTCGATCTGGCGCA TGGCCGGCGCCAACCGGATGCTGTTCGACTGCTTCGACGTGGACAGCAAGGCTGCGCGGCGTAGCGTGGCGATCCTTTCCAGCTGCCTGCGCATCGAGTG CTGGGGGCGCGATGTGGTGCTGCGGGCGTTGAACTCCAACGGACGCGCCTTGCTGGCGCCGTTGAGCGAGGACTGTCCGGCCCAGGTCACCTGCTTGCGT GACGGCGACACCCTGCACTGGCGCTTCCCCCAGGAAGAGTCGCATGCGGACGAGTGGCGACGCCTGCATGGCCTGTCCAGCCTGGAGGCGCTGCGCCGCG TGCTCGGAACGCTGGGCGACGCGGAGGGGCCCGTGCTGCTGGGCGGCCTGTTCAGCTTCGACCTGGCCGAGCAGTTCGAACCCTTGCCGGCGCCGGCCGA ACCTGCGCGGCATTGCCCGGACTACCTGTTCCTGGTGCCGGAGTTGCTGCTGGACATCGATCACCTGGCGCGCCGGACTTCGCTGCAGGCGTTCGTCCAC GATCCGGCCGGGCACGACCGGTTGGCCGCCAGCCTGCGCCAGTGTGCCGACGAATTCCATGGCGCCGTGGAGGAGGCTTCCGAGTCGCCGGTGGCAGGCG TACGGGCCGGCAACTACCAGGTCGACCTGGACGATGCGAGCTTTGCCCGCCAGGTAGAGCGCCTGCAGGCCCACGTGAGGGCCGGCGACGTGTTCCAGAT CGTACCGTCGCGCAGCTTCAGCATGCCGTGTGCGGACCCCTGGCGGGCCTATCGCCAGTTGTGCCTGCGCAACCCCAGCCCGTACCGCTTCTTCCTCGAT GCGGGGGACTTCTGCCTGTTCGGCGCTTCGCCGGAGTCGGCATTGAAGTACGACGCGGAGAGTCGCGAGGTGGAACTCTATCCCATCGCCGGCACCCGCC CGCGCGGACGCGATGCCCGGGGCGCCATCGATGCGGAACTGGACAATCGCCTGGAAGCGGAGTTGCGCCTGGACGCCAAGGAGATCGCCGAGCACATGAT GCTGGTCGACCTGGCGCGCAACGATCTGGCGCGGGTCTGCCGCAGCGGTACGCGGCAGGTGCGCGACATGCTCAAGGTCGATCGCTACAGCCACGTGATG CACCTGGTCTCGCGCGTGGCTGGCGAACTGCATGGCGAACTGGATGCGCTGCATGCCTACCGTGCCTGCCTGAACATGGGCACCTTGGTCGGTGCGCCGA AGGTCCGTGCCATGCAGTTGCTGCGGCAGTACGAAGATGGCTATCGCGGCAGCTACGGTGGCGCGATCGGCATTCTCGACAGCGCCGGCAACCTCGATAC CAGCATCGTCATCCGCTCCGCCGAGGTCCGCGAAGGTATCGCCCGGGTCCGGGCAGGCGCCGGCGTGGTGCTGGATTCGGATCCACGGCTGGAGGCCGAG GAAACCCGCAACAAGGCGCTGGCGGTGCTGACCGCCGTGGCCGCTGCCGAACGCGAAAGGGGAGAGCGCGATGCGCATCACGCTGTTGGATAA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MGARRWLVSGVGYRLEESLEYRTLVPEALSIWRMAGANRMLFDCFDVDSKAARRSVAILSSCLRIECWGRDVVLRALNSNGRALLAPLSEDCPAQVTCLR DGDTLHWRFPQEESHADEWRRLHGLSSLEALRRVLGTLGDAEGPVLLGGLFSFDLAEQFEPLPAPAEPARHCPDYLFLVPELLLDIDHLARRTSLQAFVH DPAGHDRLAASLRQCADEFHGAVEEASESPVAGVRAGNYQVDLDDASFARQVERLQAHVRAGDVFQIVPSRSFSMPCADPWRAYRQLCLRNPSPYRFFLD AGDFCLFGASPESALKYDAESREVELYPIAGTRPRGRDARGAIDAELDNRLEAELRLDAKEIAEHMMLVDLARNDLARVCRSGTRQVRDMLKVDRYSHVM HLVSRVAGELHGELDALHAYRACLNMGTLVGAPKVRAMQLLRQYEDGYRGSYGGAIGILDSAGNLDTSIVIRSAEVREGIARVRAGAGVVLDSDPRLEAE ETRNKALAVLTAVAAAERERGERDAHHAVG

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA1001 gene of Pseudomonas aeruginosa PAO1, Chromosome (1083972 to 1085564 bp)

Displaying the first five SNPs out of a total of 262 found in all isolate strains ( View all 262 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa PA7	Transition	g.1083972A>G		Not determined ATG(Met) -> ()	SNP-479202	View
Pseudomonas aeruginosa PA7	Transversion	g.1083980G>C		Not determined GCG(Ala) -> ()	SNP-479203	View
Pseudomonas aeruginosa PA14	Transition	g.1083981A>G	p.Arg4Gly	Missense AGG(Arg) -> GGG(Gly)	SNP-136680	View
Pseudomonas aeruginosa PA7	Transition	g.1083981A>G		Not determined AGG(Arg) -> ()	SNP-479204	View
Pseudomonas aeruginosa PA7	Transition	g.1083984A>G		Not determined AGG(Arg) -> ()	SNP-479205	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Structure and regulation of the anthranilate synthase genes in Pseudomonas aeruginosa: I. Sequence of trpG encoding the glutamine amidotransferase subunit.
Crawford IP, Eberly L.
Mol Biol Evol. 1986 Sep;3(5):436-48.
PMID: 3127654
Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications.
Essar DW, Eberly L, Hadero A, Crawford IP.
J Bacteriol. 1990 Feb;172(2):884-900.
PMID: 2153661