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               PA1155               
Key to diagram


View in GBrowse: 17 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name nrdB  Alternate Gene Names
Locus tag PA1155 (View 8 updates) Submit an Update GenBank Identifier (GI) 15596352 (Precomputed BLAST results at BLINK )
Genomic location 1251154 - 1249907 (-) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help No transposon mutant available yet Pseudomonas Ortholog Group Explanation of Orthologous Groups
POG001114 
Orthology uncertain because more than one top BLASTp hit was identified.
Product Information
Product Name NrdB, tyrosyl radical-harboring component of class Ia ribonucleotide reductase (Class 1  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA01156
Systomonas PA1155
RefSeq NP_249846.1
GI 15596352
KEGG PA1155
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Nucleotide biosynthesis and metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0004748 ribonucleoside-diphosphate reductase activity RCA
Biological Process
GO:0009117 nucleotide metabolic process ISS
GO:0006163 purine nucleotide metabolic process ISS
GO:0006220 pyrimidine nucleotide metabolic process ISS
GO:0009265 2'-deoxyribonucleotide biosynthetic process RCA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0208 NrdF, Ribonucleotide reductase, beta subunit [Nucleotide transport and metabolism]. Nucleotide transport and metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Pyrimidine metabolism PseudoCAP
Purine metabolism PseudoCAP
Purine metabolism KEGG pae00230 Computationally Generated
Pyrimidine metabolism KEGG pae00240 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 52% similar to ribonucleoside diphosphate reductase, beta subunit (nrdB) [Helicobacter pylori]
74% similar to Ribonucleoside Reductase, Small Chain [Chlamydia trachomatis]
Most Similar Human Homolog
ENSP00000302955, Percent Identity: 28.82%, E-Value: 4e-34, Ensembl Release: 56.
Description: Ribonucleoside-diphosphate reductase subunit M2
Structure Features
Structure Features Explanation of Structure Features Field PS00368: Ribonucleotide reductase small subunit signature
1 predicted transmembrane helices (TMHMM v.2.0)
At least one transmembrane helix predicted by Phobius
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00268 Ribonuc_red_sm, Ribonucleotide reductase, small chain. 99 387 2e-35 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region AAGAAGCTCGACGTGACCTACCGCATGGCCTGGTTCCGCGGCCTGAAGACCACCTACTACCTCCGTGCCCTGGCCGCCACCAGCACCGAGAAGTCCACCA TCAACACCGGCAAGCTCAACGCCGTGTCGGCCGGTGGCAACGACGGCCTGCAAGCCGCCCCGGCCGCCGAGCCGAAGCCGGCGCCGGTGCCGCAGGCGTG CAGCATCGATAACCCCGACTGCGAAGCCTGCCAGTGATCGCGCGTCGCTGAACGGCGTCCGTGTCGCGGCCGGGCAAGCCGGACTCCTCGCGTGGGTTCG GCCTGCCCGGCGCCTGACGGTCGCTCGTCCAGCGCCACGCTGGCAGCGCAAGGCCCCTCGCCAGGGCCAGGAACAATCCCCTTCTTCCCTCCGGGGGAGA AGAACGGCCCGGCCAGACCGTGCCGGACCACCGCTTTCGCGTGCACCTCACGCCCCATATCCAGGCCGCCGAGACGGCCGCCAGACCAGGAGAGAGCAAG

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGCTGAGCTGGGACGAATTCGACAAAGAAGACCCGACCGAAGCCAAGGCCGCGCCCGCCGCCGCCCAGGCCGTTGCCCAGGGCCATGACAAGCTGGACG ACGAAGCCGCCGGCTCGGTGGAAGAAGCGCGCGCCGTTTCCGCCGACGACTCCGACGCTGTCGCCCGCGCCAAGAAGGCCCTGAACGACCTCGACATCCA GGAAGGCTTGGACGACCTCGAAGGCTCCGCCGCGCGCGTGCAGGTCGGCGACAAGCAGATGATCAACGCCCGTGCCGACCTCAACCAGCTCGTTCCCTTC AAGTACGACTGGGCCTGGCAGAAGTATCTGGATGGTTGCGCCAACCACTGGATGCCGCAGGAAGTCAACATGAACGCCGACATCGCCCTGTGGAAGAGCA AGGACGGCCTCAGCGAAGACGAGCGGCGCATCGTCATGCGCAACCTCGGCTTCTTCTCCACCGCCGACTCGCTGGTCGCCAACAACCTGGTGCTGGCCGT GTACCGCCTGATCACCAACCCCGAGTGCCGCCAGTACATCCTGCGCCAGGCCTTCGAAGAGGCGATCCACACCCACGCCTACCAGTACTGCATCGAGTCG CTGGGCATGGACGAGGGCGAGATCTTCAACATGTACCACGAGATCCCCAGCGTGGCGAAGAAGGCCTCCTGGGGCCTGAAGTACACCCGCTCGATCTCCG ACCCGATGTTCCAGACCGGCACCCCGGAAACCGACCGCCAGTTCCTGCGCAACCTGATCGCCTACTACTGCGTGCTGGAAGGCATCTTCTTCTACTGCGG CTTCACCCAGATCCTCTCCATGGGCCGCCGCAACAAGATGACCGGCACCGCCGAGCAGTTCCAGTACATCCTCCGCGACGAGTCGATGCACCTGAACTTC GGTATCGACGTGATCAACCAGATCAAGATCGAGAACCCGCACCTGTGGGACGCCCAGATGAAGGACGAGGCGACCCAGATGATCCTCCAGGGCACCCAGC TGGAGATCGAATACGCGCGCGACACCATGCCGCGCGGGGTGCTGGGCATGAACGCGGCGATGATGGAGGACTACCTGAAGTTCATCGCCAACCGGCGCCT GACCCAGATCGGCCTGAAGGAAGAGTATCCGGGGACCACCAACCCGTTCCCGTGGATGTCGGAGATCATGGACCTGAAGAAGGAGAAGAACTTCTTCGAG ACGCGGGTTATCGAGTACCAGACGGGCGGAGCACTGAGCTGGGATTGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MLSWDEFDKEDPTEAKAAPAAAQAVAQGHDKLDDEAAGSVEEARAVSADDSDAVARAKKALNDLDIQEGLDDLEGSAARVQVGDKQMINARADLNQLVPF KYDWAWQKYLDGCANHWMPQEVNMNADIALWKSKDGLSEDERRIVMRNLGFFSTADSLVANNLVLAVYRLITNPECRQYILRQAFEEAIHTHAYQYCIES LGMDEGEIFNMYHEIPSVAKKASWGLKYTRSISDPMFQTGTPETDRQFLRNLIAYYCVLEGIFFYCGFTQILSMGRRNKMTGTAEQFQYILRDESMHLNF GIDVINQIKIENPHLWDAQMKDEATQMILQGTQLEIEYARDTMPRGVLGMNAAMMEDYLKFIANRRLTQIGLKEEYPGTTNPFPWMSEIMDLKKEKNFFE TRVIEYQTGGALSWD

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA1155 gene of Pseudomonas aeruginosa PAO1, Chromosome (1249907 to 1251154 bp)


Displaying the first five SNPs out of a total of 57 found in all isolate strains ( View all 57 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA14 Transition g.1249921G>A
Silent
CTG(Leu) -> TTG(Leu) 		SNP-131996 View
Pseudomonas aeruginosa PA14 Transversion g.1249922T>G
Silent
GCA(Ala) -> GCC(Ala) 		SNP-131997 View
Pseudomonas aeruginosa PA14 Transversion g.1249925T>A
Silent
GGA(Gly) -> GGT(Gly) 		SNP-131998 View
Pseudomonas aeruginosa LESB58 Transversion g.1250108G>C
Silent
GGC(Gly) -> GGG(Gly) 		SNP-72676 View
Pseudomonas aeruginosa PACS2 Transversion g.1250108G>C
Silent
GGC(Gly) -> GGG(Gly) 		SNP-127593 View
Select to view SNPs in a specific strain
SNP Map
PubMed Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE.
Genome Res. 2005 Feb;15(2):321-9.
PMID: 15687295
Ribonucleotide reductase modularity: Atypical duplication of the ATP-cone domain in Pseudomonas aeruginosa.
Torrents E, Westman M, Sahlin M, Sjoberg BM.
J Biol Chem. 2006 Sep 1;281(35):25287-96. Epub 2006 Jul 7.
PMID: 16829681
Identification and isolation of the gene encoding the small subunit of ribonucleotide reductase from Saccharomyces cerevisiae: DNA damage-inducible gene required for mitotic viability.
Elledge SJ, Davis RW.
Mol Cell Biol. 1987 Aug;7(8):2783-93.
PMID: 3313004
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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