Pseudomonas Genome Database - Annotation for PA1156

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PA1156

Key to diagram

View in GBrowse:

16 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name	nrdA	Alternate Gene Names
Locus tag	PA1156 (View 6 updates) Submit an Update	GenBank Identifier (GI)	15596353 (Precomputed BLAST results at BLINK )
Genomic location	1254309 - 1251418 (-)	Feature type	CDS
Transposon Mutant Available?	No transposon mutant available yet	Pseudomonas Ortholog Group	POG001115
Product Information
Product Name	NrdA, catalytic component of class Ia ribonucleotide reductase (Class 1 )
Alternate Product Names
Subcellular localization	Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA01157
Systomonas	PA1156
RefSeq	NP_249847.1
GI	15596353
KEGG	PA1156
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Nucleotide biosynthesis and metabolism

GO Accession

GO Term

Evidence

Molecular Function

GO:0004748	ribonucleoside-diphosphate reductase activity	RCA
GO:0016959	class I ribonucleotide reductase activity	IEA

Biological Process

GO:0009117	nucleotide metabolic process	ISS
GO:0009265	2'-deoxyribonucleotide biosynthetic process	RCA
GO:0009265	2'-deoxyribonucleotide biosynthetic process	IEA

Cellular Component

GO:0005971

ribonucleoside-diphosphate reductase complex

IEA

COG Prediction

Accession	Description	Category
COG0209	NrdA, Ribonucleotide reductase, alpha subunit [Nucleotide transport and metabolism].	Nucleotide transport and metabolism

Pathways

Name	Database	Xref
Pyrimidine metabolism	PseudoCAP
Purine metabolism	PseudoCAP
Purine metabolism	KEGG	pae00230	Computationally Generated
Pyrimidine metabolism	KEGG	pae00240	Computationally Generated
Metabolic pathways	KEGG	pae01100	Computationally Generated

Reactions

EC Number

1.17.4.1

Homology

BLINK

Precomputed BLAST results at BLINK

Most Similar Human Homolog

ENSP00000300738, Percent Identity: 40.69%, E-Value: 2e-152, Ensembl Release: 56.
Description: Ribonucleoside-diphosphate reductase large subunit

Structure Features

Ribonucleotide reductase large subunit signature BL00089

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF03477	ATP-cone, ATP cone domain.	32	129	1e-15	23.0
PF00317	Ribonuc_red_lgN, Ribonucleotide reductase, all-alpha domain.	293	368	7e-21	23.0
PF02867	Ribonuc_red_lgC, Ribonucleotide reductase, barrel domain.	370	907	1e-155	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

ACTCACGCACAGAAGACCCACACTTTATCCACAGGTTTCCCCCAGACTGTCACCTTGCAAACACCCCATTAGCGCATTATCTTGTATCCCCATCGTCGCC ACCCCCTATATCTTGGGTTTCGAGCGCACGAACGGCTACAACGGAACCAACCGCGCACGGACCACTCTGATGGCTCCGGCGGCTCCGCAAAAAAGCATTT TCCAGACGTACTGAAAGCATCGACGGCGCTCATCGCAAGGAGCCCTTCATGACGTTGGAAAAGCGGTACGGGTGTTGCAACGGACGCTGTAAACAGACAG TCAACACCACTAGGTATTGTGTTCGACGGCAAAACGCCACGAACCCGCCAGGGCCAGGGAGGCACTCACAGTCCCCACCCTCAGCATTCGAATGAAGCAG CCGCCGCGAGGCCGGCTGCCCGGCTCTTTATCGATTCAAGGGAATGGGCGCCCCGGCGCCCGCTAGCAAAACCATTACGAGAACGTGGAGATACCCACCC

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGCATACCGACACCACACGCGAGAACCCGCAGGCCAGCGCGCCGCAGGCCGCCGATTCGTCGCAGGATCTGGCCGCCACCGCGCCGGGCCAGCTACGCG TGATCAAGCGCAACGGCACCGTAGTCCCCTACACCGATGACAAGATCACCGTCGCCATCACCAAGGCGTTCCTCGCCGTGGAAGGCGGCACCGCCGCCGC CTCGTCGCGTATCCATGACACCGTGCGCCGCCTGACCGAGCAGGTCACCGCCACCTTCAAGCGCCGCATGCCGTCCGGCGGCACCATCCACATCGAAGAG ATCCAGGACCAGGTCGAACTGGCCCTGATGCGCGCCGGCGAGCAGAAGGTCGCCCGCGACTACGTGATCTACCGCGAAGCCCGCGCCGCCGAGCGCAAGA ACGCCGGCGCCGCCAGCGACGTCGCCCAGCCGCACCCGAGCATCCGCATCACCCGCGCCGACGGCAGCCTGTCGCCGCTGGACATGGGCCGCCTGAACAC CATCATCAGCGAAGCCTGCGAAGGCCTGGCCGAGGTGGACGGCGCGCTGATCGAGCGCGAGACCCTGAAGAACCTCTACGACGGCGTCGCCGAGAAGGAC GTCAACACCGCCCTGGTGATGACCGCGCGCACCCTGGTCGAGCGCGAGCCGAACTACTCCTACGTCACCGCCCGCCTGCTGATGGACACCCTGCGCGCCG AAGCCCTGGGCTTCCTCGGCGTGGCCGAGAGCGCCACCCATCACGAGATGGCCGAGCTGTACGCCAAGGCCCTGCCGGCCTACATCGAGAAAGGCGCCGA GTTCGAGCTGGTCGATGCCAAGCTGAAGGAATTCGACCTGGAGAAACTGGGCAAGGCCATCGATCACGAGCGCGACCAGCAGTTCACCTACCTCGGCCTG CAGACCCTGTACGACCGCTACTTCATCCACAAGGACGGCATCCGCTTCGAACTGCCGCAGATCTTCTTCATGCGCGTGGCCATGGGCCTGGCCATCGAGG AGAAGGACCGCGAGGCACGTGCCATCGAGTTCTACAACCTGCTGTCGTCCTTCGACTACATGAGTTCCACCCCCACCCTGTTCAACGCCGGCACCCTGCG TCCGCAGCTCTCCAGCTGCTACCTGACCACCGTGCCGGACGACCTGTCGGGCATCTACGGCGCCATCCACGACAACGCCATGCTGTCGAAATTCGCCGGC GGCCTGGGCAACGACTGGACCCCGGTGCGCGCGCTGGGCTCCTACATCAAGGGCACCAACGGCAAGTCCCAGGGCGTCGTGCCCTTCCTGAAAGTGGTCA ATGACACCGCGGTCGCGGTCAACCAGGGCGGCAAGCGCAAGGGCGCGGTCTGCGCCTACCTGGAAACCTGGCACCTGGACATCGAGGAATTCCTCGAGCT GCGCAAGAACACCGGTGACGACCGTCGTCGTACCCACGACATGAACACCGCCAACTGGATCCCCGACCTGTTCATGAAGCGCGTCTTCGACGACGGCTCC TGGACCCTCTTCTCGCCGTCCGACGTCCCCGACCTGCACGACCTCTACGGCAAGGCCTTCGAGGAGCGCTACGAGTACTACGAGGCCCTGGCCAGCTACG GCAAGCTGAAGCTGCACAAGGTCGTCCAGGCCAAGGACCTGTGGCGCAAGATGCTCTCCATGCTGTTCGAGACCGGCCACCCATGGCTGACCTTCAAGGA CCCGTGCAACCTGCGCAGCCCGCAGCAGCACGTGGGCGTGGTGCACAGCTCGAACCTCTGCACCGAGATCACCCTGAACACCAACAAGGACGAGATCGCC GTCTGCAACCTGGGCTCGATCAACCTGGTCAACCACATCGTCGACGGCAAGCTGGACACCGCCAAGCTGGAGAAGACCGTCAAGACCGCGGTACGCATGC TCGATAACGTCATCGACATCAACTACTACTCGGTCCCGCAGGCGCAGAACTCCAACTTCAAGCACCGTCCGGTCGGCCTGGGCATCATGGGCTTCCAGGA CGCCCTGTACCTGCAGCACATCCCCTACGGTTCGGACGCGGCCATCGCCTTCGCCGACCAGTCCATGGAAGCCATCAGCTACTACGCGATCCAGGCTTCC TGCGACCTGGCCGACGAGCGCGGCGCCTACCAAACCTTCCAGGGCTCGCTGTGGTCGCAAGGCATCCTGCCGATCGACTCGGAGAAGAAGCTGATCGAAG AGCGCGGCGCCAAGTACATCGAAGTCGACCTGTCGGAAACCCTCGACTGGGCGCCGCTGCGCGAGCGCGTACAGAAAGGCATCCGCAACTCGAACATCAT GGCCATCGCGCCGACCGCGACCATCGCCAACATCACCGGCGTCTCGCAGTCGATCGAGCCGACCTACCAGAACCTGTACGTGAAGTCGAACCTCTCCGGC GAGTTCACCGTGATCAACCCCTACCTGGTGCGCGACCTCAAGGCCCGCGGCCTGTGGGACCCGGTCATGGTCAACGACCTGAAGTACTACGACGGTTCCG TGCAGCAGATCGAGCGCATCCCGCAGGACCTCAAGGACCTCTACGCCACCGCGTTCGAAGTCGAGACCCGCTGGATCGTCGAAGCCGCCAGCCGTCGCCA GAAGTGGATCGACCAGGCCCAGTCGCTGAACCTGTACATCGCCGGCGCCTCGGGCAAGAAGCTCGACGTGACCTACCGCATGGCCTGGTTCCGCGGCCTG AAGACCACCTACTACCTCCGTGCCCTGGCCGCCACCAGCACCGAGAAGTCCACCATCAACACCGGCAAGCTCAACGCCGTGTCGGCCGGTGGCAACGACG GCCTGCAAGCCGCCCCGGCCGCCGAGCCGAAGCCGGCGCCGGTGCCGCAGGCGTGCAGCATCGATAACCCCGACTGCGAAGCCTGCCAGTGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MHTDTTRENPQASAPQAADSSQDLAATAPGQLRVIKRNGTVVPYTDDKITVAITKAFLAVEGGTAAASSRIHDTVRRLTEQVTATFKRRMPSGGTIHIEE IQDQVELALMRAGEQKVARDYVIYREARAAERKNAGAASDVAQPHPSIRITRADGSLSPLDMGRLNTIISEACEGLAEVDGALIERETLKNLYDGVAEKD VNTALVMTARTLVEREPNYSYVTARLLMDTLRAEALGFLGVAESATHHEMAELYAKALPAYIEKGAEFELVDAKLKEFDLEKLGKAIDHERDQQFTYLGL QTLYDRYFIHKDGIRFELPQIFFMRVAMGLAIEEKDREARAIEFYNLLSSFDYMSSTPTLFNAGTLRPQLSSCYLTTVPDDLSGIYGAIHDNAMLSKFAG GLGNDWTPVRALGSYIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVCAYLETWHLDIEEFLELRKNTGDDRRRTHDMNTANWIPDLFMKRVFDDGS WTLFSPSDVPDLHDLYGKAFEERYEYYEALASYGKLKLHKVVQAKDLWRKMLSMLFETGHPWLTFKDPCNLRSPQQHVGVVHSSNLCTEITLNTNKDEIA VCNLGSINLVNHIVDGKLDTAKLEKTVKTAVRMLDNVIDINYYSVPQAQNSNFKHRPVGLGIMGFQDALYLQHIPYGSDAAIAFADQSMEAISYYAIQAS CDLADERGAYQTFQGSLWSQGILPIDSEKKLIEERGAKYIEVDLSETLDWAPLRERVQKGIRNSNIMAIAPTATIANITGVSQSIEPTYQNLYVKSNLSG EFTVINPYLVRDLKARGLWDPVMVNDLKYYDGSVQQIERIPQDLKDLYATAFEVETRWIVEAASRRQKWIDQAQSLNLYIAGASGKKLDVTYRMAWFRGL KTTYYLRALAATSTEKSTINTGKLNAVSAGGNDGLQAAPAAEPKPAPVPQACSIDNPDCEACQ

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA1156 gene of Pseudomonas aeruginosa PAO1, Chromosome (1251418 to 1254309 bp)

Displaying the first five SNPs out of a total of 83 found in all isolate strains ( View all 83 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa C3719	Transition	g.1251445A>G		Silent GAT(Asp) -> GAC(Asp)	SNP-37219	View
Pseudomonas aeruginosa 2192	Transition	g.1251445A>G		Silent GAT(Asp) -> GAC(Asp)	SNP-40610	View
Pseudomonas aeruginosa LESB58	Transition	g.1251445A>G		Silent GAT(Asp) -> GAC(Asp)	SNP-72686	View
Pseudomonas aeruginosa PACS2	Transition	g.1251445A>G		Silent GAT(Asp) -> GAC(Asp)	SNP-127603	View
Pseudomonas aeruginosa PA7	Transition	g.1251445A>G		Silent GAT(Asp) -> GAC(Asp)	SNP-255383	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Ribonucleotide reductase modularity: Atypical duplication of the ATP-cone domain in Pseudomonas aeruginosa.
Torrents E, Westman M, Sahlin M, Sjoberg BM.
J Biol Chem. 2006 Sep 1;281(35):25287-96. Epub 2006 Jul 7.
PMID: 16829681
Promoter identification and expression analysis of Salmonella typhimurium and Escherichia coli nrdEF operons encoding one of two class I ribonucleotide reductases present in both bacteria.
Jordan A, Aragall E, Gibert I, Barbe J.
Mol Microbiol. 1996 Feb;19(4):777-90.
PMID: 8820648
Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis.
Stephens RS, Kalman S, Lammel C, Fan J, Marathe R, Aravind L, Mitchell W, Olinger L, Tatusov RL, Zhao Q, Koonin EV, Davis RW.
Science. 1998 Oct 23;282(5389):754-9.
PMID: 9784136