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               PA2001               
Key to diagram


View in GBrowse: (?) 16 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name atoB  Alternate Gene Names
Locus tag PA2001 (View 6 updates) GenBank Identifier (GI) 15597197 (Precomputed BLAST results at BLINK )
Genomic location 2187065 - 2188246 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name acetyl-CoA acetyltransferase (Class 2  Explanation of Product Name Confidence)
Alternate Product Names acetoacetyl-CoA thiolase
Subcellular localization
Cytoplasmic (Class 2Explanation of Localization Confidence)
Cytoplasmic (Class 3Explanation of Localization Confidence)
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Central intermediary metabolism
Fatty acid and phospholipid metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0008137 NADH dehydrogenase (ubiquinone) activity ISS
GO:0003985 acetyl-CoA C-acetyltransferase activity IEA
Biological Process
GO:0008152 metabolic process ISS
GO:0044255 cellular lipid metabolic process ISS
GO:0006631 fatty acid metabolic process IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0183 PaaJ, Acetyl-CoA acetyltransferase [Lipid metabolism]. Lipid metabolism
TIGRFAM predictionExplanation of TIGRFAM predictions
Accession Name Function Significance Role Sub Role Release No.
TIGR01930 AcCoA-C-Actrans acetyl-CoA acetyltransferases 8.3e-213 Fatty acid and phospholipid metabolism Other 8.0
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Tryptophan metabolism PseudoCAP
Pyruvate metabolism PseudoCAP
Propanoate metabolism PseudoCAP
Lysine degradation PseudoCAP
Butanoate metabolism PseudoCAP
Fatty acid biosynthesis (path 2) PseudoCAP
Fatty acid metabolism PseudoCAP
lysine degradation I PseudoCyc LYSINE-DEG1-PWY
tryptophan degradation III PseudoCyc TRYPTOPHAN-DEGRADATION-1
Fatty acid metabolism KEGG pae00071 Computationally Generated
Synthesis and degradation of ketone bodies KEGG pae00072 Computationally Generated
Valine, leucine and isoleucine degradation KEGG pae00280 Computationally Generated
Lysine degradation KEGG pae00310 Computationally Generated
Tryptophan metabolism KEGG pae00380 Computationally Generated
Pyruvate metabolism KEGG pae00620 Computationally Generated
Benzoate degradation via CoA ligation KEGG pae00632 Computationally Generated
Propanoate metabolism KEGG pae00640 Computationally Generated
Butanoate metabolism KEGG pae00650 Computationally Generated
Terpenoid backbone biosynthesis KEGG pae00900 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Two-component system KEGG pae02020 Computationally Generated
Reactions
EC Number 2.3.1.9
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 74% similar to acetyl-CoA acetyltransferase, atoB gene product [Escherichia coli]
Most Similar Human Homolog
ENSP00000356015, Percent Identity: 52.55%, E-Value: 6e-100, Ensembl Release: 56.
Description: Acetyl-CoA acetyltransferase, cytosolic
Structure Features
Structure Features Explanation of Structure Features Field thiolases acyl-enzyme intermediate signature BL00098, PDOC00092
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00108 Thiolase_N, Thiolase, N-terminal domain. 3 263 1e-113 23.0
PF00109 ketoacyl-synt, Beta-ketoacyl synthase, N-terminal domain. 7 125 2e-05 23.0
PF02803 Thiolase_C, Thiolase, C-terminal domain. 270 392 2e-50 23.0
 
Genomic Context
Genomic context Explanation of Genomic Context Field - clustered with scoAB and atoE
Microarray Expression Data
NCBI GEO Link
Sequence data
Upstream 500 BP Region CGGTGCCTTCGAGGTGGACGTGCGCGGCAACATCGCCTCCTGGATGATCCCCGGCAAGCTGGTCAAGGGCATGGGCGGCGCCATGGACCTGGTGGCCGGC GCCGAGAACATCATCGTCACCATGACCCACGCCTCCAAGGACGGCGAGTCCAAGCTGCTGCCGTACTGCACCCTGCCGCTGACCGGTTGCGGCTGCATTC GCAAGGTGCTCACCGACCTGGCCTACCTGGAGATCGAGGACGGCGCCTTCATCCTCCGCGAGCGCGCCCCCGGGGTCAGCGTCGAGGAGATCGTGGCGAA GACCGCCGGCAAGCTGATCGTCCCCGAGCACGTGCCGGAAATGAGTTTCTGAATCGCGGCCCGGCGGCCGGCGCCTCCACGCAAGGCGCCGGCCGCGCGG ACCGTCCTTTCCAGAGGGCGTAGGATAGGAGCCTGTCGCACCGCCTACGTCCACCCGCAGCGATTCGGCCCGGCCACGGGCGAATCCAGGAGAACCATCC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGCAAGACGTCGTCATCGTCGCCGCTACCCGTACCGCCGTCGGCAGCTTCCAGGGCTCGCTGGCCGGCATTCCCGCCCCCGAGCTGGGCGCCGCGGTAA TCCGCCGCCTGCTCGAACAGACCGGTCTCGATGCCGGCCAGGTCGACGAAGTGATCCTCGGCCAGGTGCTCACCGCCGGGTCCGGGCAGAACCCGGCGCG CCAGGCGGCGATCAAGGCCGGCCTGCCGGTCGGCGTGCCGGCCATGACCCTGAACAAGGTCTGCGGCTCCGGCCTCAAGGCCCTGCACCTGGGCGCCCAG GCGATCCGCTGCGGCGACGCCGAGGTGATCGTCGCCGGCGGCCAGGAGAACATGAGCCTGGCGCCCTACGTCATGCCCGGCGCCCGCACCGGCCTGCGCA TGGGCCATGCGAAGCTGGTCGACAGCATGATCGAGGACGGCCTGTGGGACGCCTTCAACGACTACCACATGGGCATCACCGCCGAGAACCTGGCGGAGAA GTACGGCCTGACCCGCGAGGAGCAGGACGCCTTCGCCGCCGCTTCGCAGCAGAAGGCCATCGCCGCCATCGAGGGCGGGCGCTTCCGCGACGAGATCACG CCGATCCAGGTCCCGCAGCGCAAGGGCGAGCCGCTGGGCTTCGATACCGACGAGCAGCCGCGCGCCGGCACCACCGTCGAGGCCCTGGCCAAGCTCAAGC CGGCCTTCAGGAAGGACGGCAGCGTCACCGCCGGCAACGCCTCCAGCCTCAACGACGGCGCCGCCGCGGTATTGCTGATGAGCGCGGCCAAGGCCAAGGC CCTGGGCCTGCCGGTGCTGGCGCGGATCGCCAGCTACGCCAGCGCCGGGGTCGACCCGGCGATCATGGGCATCGGCCCGGTCTCCGCCACCCGTCGCGCG CTCGACAAGGCCGGCTGGAGCCTGGAGCAACTGGACCTGATCGAGGCCAACGAGGCCTTCGCCGCGCAGTCCCTGGCGGTGGGCCGGGAACTGGGCTGGG ATGCCGCCAGGGTCAACGTCAACGGCGGCGCCATCGCCCTTGGCCACCCGATCGGCGCGTCCGGCTGCCGGGTGCTGGTGACCCTGCTGCACGAGATGAT CCGTCGCGACGCGAAGAAGGGCCTCGCCACCCTGTGCATCGGCGGCGGGCAGGGCGTCGCCCTGACCCTGGCCCGCGACTGA

BLAST search against this genome or other genomes in database


Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence MQDVVIVAATRTAVGSFQGSLAGIPAPELGAAVIRRLLEQTGLDAGQVDEVILGQVLTAGSGQNPARQAAIKAGLPVGVPAMTLNKVCGSGLKALHLGAQ AIRCGDAEVIVAGGQENMSLAPYVMPGARTGLRMGHAKLVDSMIEDGLWDAFNDYHMGITAENLAEKYGLTREEQDAFAAASQQKAIAAIEGGRFRDEIT PIQVPQRKGEPLGFDTDEQPRAGTTVEALAKLKPAFRKDGSVTAGNASSLNDGAAAVLLMSAAKAKALGLPVLARIASYASAGVDPAIMGIGPVSATRRA LDKAGWSLEQLDLIEANEAFAAQSLAVGRELGWDAARVNVNGGAIALGHPIGASGCRVLVTLLHEMIRRDAKKGLATLCIGGGQGVALTLARD

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
References
PubMed Genetic and molecular characterization of the genes involved in short-chain fatty acid degradation in Escherichia coli: the ato system.
Jenkins LS, Nunn WD.
J Bacteriol. 1987 Jan;169(1):42-52.
PMID: 3025185
 
Alternate Annotations and Cross-References
TIGR NTL03PA02001
Systomonas PA2001
RefSeq NP_250691.1
GI 15597197
KEGG PA2001
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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