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               PA2023               
Key to diagram


View in GBrowse: (?) 16 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name galU  Alternate Gene Names gtaB, hasC
Locus tag PA2023 (View 7 updates) GenBank Identifier (GI) 15597219 (Precomputed BLAST results at BLINK )
Genomic location 2215102 - 2215941 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name UTP--glucose-1-phosphate uridylyltransferase (Class 2  Explanation of Product Name Confidence)
Alternate Product Names glucose-1-phosphate uridylyltransferase, UDP-glucose pyrophosphorylase
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Central intermediary metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0008662 1-phosphofructokinase activity ISS
GO:0003983 UTP:glucose-1-phosphate uridylyltransferase activity IEA
Biological Process
GO:0008152 metabolic process ISS
GO:0009225 nucleotide-sugar metabolic process IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG1210 GalU, UDP-glucose pyrophosphorylase [Cell envelope biogenesis, outer membrane]. Cell envelope biogenesis, outer membrane
TIGRFAM predictionExplanation of TIGRFAM predictions
Accession Name Function Significance Role Sub Role Release No.
TIGR01099 galU UTP-glucose-1-phosphate uridylyltransferase 3.4e-188 Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 8.0
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Starch and sucrose metabolism PseudoCAP
Pentose and glucuronate interconversions PseudoCAP
Galactose metabolism PseudoCAP
Nucleotide sugars metabolism PseudoCAP
galactose metabolism PseudoCyc GALACTMETAB-PWY
UDP-glucose conversion PseudoCyc P61-PWY
colanic acid building blocks biosynthesis PseudoCyc COLANSYN-PWY
glucose 1-phosphate metabolism PseudoCyc GLUCOSE1PMETAB-PWY
Pentose and glucuronate interconversions KEGG pae00040 Computationally Generated
Galactose metabolism KEGG pae00052 Computationally Generated
Starch and sucrose metabolism KEGG pae00500 Computationally Generated
Amino sugar and nucleotide sugar metabolism KEGG pae00520 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 2.7.7.9
Enzyme Assays
PDF file(s) provided by Sigma-Aldrich:
 Uridine-5'-Diphosphoglucose Pyrophosphorylase (2.7.7.9)
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 69% similar to hasC gene product of [Streptococcus pyogenes]
Most Similar Human Homolog
ENSP00000311130, Percent Identity: 24.72%, E-Value: 2e-10, Ensembl Release: 56.
Description: Mannose-1-phosphate guanyltransferase beta
Structure Features
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00483 NTP_transferase, Nucleotidyl transferase. 9 267 5e-25 23.0
 
Microarray Expression Data
NCBI GEO Link
Sequence data
Upstream 500 BP Region GAGACCCTCGGCGAGCTGTTCCAGGGACGCTGGCAAGGGCGCACGGTGGCGCTCTGGGGGCTGGCCTTCAAGCCGGGTACCGACGACCTGCGCGAGGCGC CGAGCCTGGTCCTGCTGGAGGCGCTGCTGCGCCACGGGGTGCGGGTCAGGGCCCATGATCCGGTGGCCAACGCCGGGGTCGCGGCACGCTATCCGGAAGC TGTGGCCTGCGCCCGATTGACGCTCCACGACTCGCCCTACGCGGCCGTCGAGGGCGCCGATGCCCTGGTGCTGGTGACCGAGTGGAAACAGTTCCGCCAG CCGGACTTCCAGAAAATCCGTGGATCCATGCGAACTCCCCTGTTGGTCGACGGTCGGAATCTCTACGCACCGGCGCGGATGGCGGAACTGGGCTTCATCT ACCAGGGCATAGGACGTCCAAGGGCCGGGCATTGTAAGGCGAGCGCAGCCTGATTAGACTGCGCGCCATCCGCATACTTCGTCCATGTCGAAGGATTCTC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGATCAAGAAATGTCTTTTCCCGGCCGCCGGTTACGGCACCCGTTTCCTCCCCGCCACCAAGGCCATGCCCAAGGAAATGCTGCCGGTGGTGAACAAGC CGCTGATCCAGTACGCGGTGGAGGAGGCGCTGGAAGCCGGCCTTTCCGAGATCGGCATCGTCACCGGCCGCGGCAAGCGTTCGCTGGAAGACCACTTCGA CATCAGCTACGAGCTGGAACACCAGATCCGCAACACCGACAAGGAAAAGTACCTGGTCGGCATCCGTCGGCTGATCGACGAGTGCACCTTCGCCTACACC CGCCAGGTGGAGATGAAGGGCCTCGGCCACGCCATCCTCACCGGTCGTCCGCTGATCGGCGACGAGCCGTTCGCCGTGGTCCTGGCCGACGACCTGTGCC TGAACCTCGAAGGCGACAGCGTGCTGAAGCAGATGGTCAAGCTGTACAACCAGTTCCGCTGCTCCATCGTGGCGATCCAGGAAGTGCCGCCGGAAGAGAC CAACAAGTACGGCGTGATCGCCGGCGAGATGATCCGCGACGATATCTTCCGGGTGAACACCATGGTCGAGAAGCCGAAGCCGGAAGAGGCGCCGTCGAAC CTGGCGATCATCGGCCGCTACATCCTGACCCCGGACATCTTCGACCTGATCGAGCAGACTGAACCGGGCAAGGGCGGCGAGATCCAGATCACCGATGCCC TGATGAAGCAGGCCCAGGACGGCTGCGTGCTGGCCTACAAGTTCAAGGGCAAGCGTTTCGACTGCGGCAGCGCCGAGGGTTACATCGAGGCGACCAACTT CTGCTACGAAAACCTCTACAAGACCGGCAAGGCTCACTGA

BLAST search against this genome or other genomes in database


Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence MIKKCLFPAAGYGTRFLPATKAMPKEMLPVVNKPLIQYAVEEALEAGLSEIGIVTGRGKRSLEDHFDISYELEHQIRNTDKEKYLVGIRRLIDECTFAYT RQVEMKGLGHAILTGRPLIGDEPFAVVLADDLCLNLEGDSVLKQMVKLYNQFRCSIVAIQEVPPEETNKYGVIAGEMIRDDIFRVNTMVEKPKPEEAPSN LAIIGRYILTPDIFDLIEQTEPGKGGEIQITDALMKQAQDGCVLAYKFKGKRFDCGSAEGYIEATNFCYENLYKTGKAH

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
References
PubMed Molecular characterization of hasC from an operon required for hyaluronic acid synthesis in group A streptococci. Demonstration of UDP-glucose pyrophosphorylase activity.
Crater DL, Dougherty BA, van de Rijn I.
J Biol Chem. 1995 Dec 1;270(48):28676-80.
PMID: 7499387
The gene encoding UDP-glucose pyrophosphorylase is required for the synthesis of xanthan gum in Xanthomonas campestris.
Wei CL, Lin NT, Weng SF, Tseng YH.
Biochem Biophys Res Commun. 1996 Sep 24;226(3):607-12.
PMID: 8831665
 
Alternate Annotations and Cross-References
TIGR NTL03PA02023
Systomonas PA2023
RefSeq NP_250713.1
GI 15597219
KEGG PA2023
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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