Pseudomonas Genome Database - Annotation for PA2237

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PA2237

Key to diagram

View in GBrowse: (?)

10 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Gene Information

Strain

Pseudomonas aeruginosa PAO1 (Tax ID: 208964)

Replicon

Chromosome(5651 genes, 6264404 bp) [ RefSeq ]

Gene name

pslG

Alternate Gene Names

Locus tag

PA2237 (View 8 updates)

GenBank Identifier (GI)

15597433 (Precomputed BLAST results at BLINK )

Genomic location

2461460 - 2462788 (+)

Feature type

CDS

Transposon Mutant Available?

Yes (View in GBrowse)

Pseudomonas Ortholog Group

POG003249

Product Information

Product Name

probable glycosyl hydrolase (Class 3

)

Alternate Product Names

Subcellular localization

Cytoplasmic Membrane (Class 3

)

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Putative enzymes

Pathways

Name	Database	Xref
Starch and sucrose metabolism	KEGG	pae00500	Computationally Generated
Amino sugar and nucleotide sugar metabolism	KEGG	pae00520	Computationally Generated
Metabolic pathways	KEGG	pae01100	Computationally Generated

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

43% similar to putative GlcNAc transferase [Bordetella]

Structure Features

PS01027: Glycosyl hydrolases family 39 active site
One predicted transmembrane domain
type I export signal computationally predicted by LipoP v.1.0 (cleavage after residue 30)
type I export signal computationally predicted by Phobius (cleavage after residue 30)
type I export signal computationally predicted by SignalP v.3.0 Hidden Markov Models (cleavage after residue 30)

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF01229	Glyco_hydro_39, Glycosyl hydrolases family 39.	59	198	5e-05	23.0

Genomic Context

Genomic context

- Part of pslABCDEFGHIJKLMNO cluster involved in biofilm development.

Operons/Regulons

Type

operon

Genes

Gene	Product
pslA	PslA
pslB	probable phosphomannose isomerase/GDP-mannose pyrophosphorylase
pslC	probable glycosyl transferase
pslD	PslD
pslE	hypothetical protein
pslF	hypothetical protein
pslG	probable glycosyl hydrolase
pslH	hypothetical protein
pslI	probable transferase
pslJ	hypothetical protein
pslK	hypothetical protein
pslL	hypothetical protein

Evidence

Computationally Predicted

PubMed

18988623

Cross-Reference

DOOR-13575

Comments

psl cluster plays a role in cell-cell and/or cell-surface interaction in biofilm formation.

Microarray Expression Data

NCBI GEO

Link

Sequence data

Upstream 500 BP Region

CCTGACCCTGGCCGGCGGCACCGCCGCGGAAATGGCCTTCGGCGCCGGCGGCAACTACCTCTACCAGTTGAAGGCACAGATCGCCGAGCTTGGCCTGGCC GACGCCATCGACTGGAACCTGAACCTGGCCGCCGCGGACATCCCGCGTACCATCCAGGCCCACCACGTGATGGTCCTGCCGTACCGCGAGTCGAAGAAAC TCGGTCTGCTCGGCCAGCAGCGCGGCACCAGCGGCGCCCTGTCCTGGGCGGCAGCCTGCGGGCGCGGCGCGATCACCTCGGACGCTCGCGCCTTCGCCGA GGAAGTCGCCAGCGGCAACGGCGCCATCTACCCTCAGGGAGACGTGGCAGCCCTCTCCGAACAGTTGTTGAACCTCGCTCGCGAACCCCGCCTGGCACAG GCCTGGGCCGAGCGCGCCGGAGCGATCGGCCATGCGCGGACCTGGCCGCGCACCGCGCTGCGCTTCCAGGCACTGTTCAACGACATGGTGGGAACCGTTC

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGGCACGTAAGGGACTCTATCTGGGCGGCAGCGCGCTGCTGCTCGCCGTGGTACTGCTGCTGGTGTTCTGGGGGCGTCCCGCCGACGCCGAGATCCAGG TACTGAAGGCGCCTCGCGCGGTGGTCTGGAAAGACTTCCTCGGGGTCAACGCGCAGTTCCTCTGGTTCAGCCCGGAGCGTTACAACAAGCAGATCGACCG CCTGCAGGACCTGGGGCTGGAGTGGGTGCGCCTGGACCTGCACTGGGACCGCCTGGAAACCGCCGAGGACCAGTACCAGCTGGCCTCCCTCGACCAGTTG GTCAAAGATCTCGAGGCGCGCCAGCTGAAGTCGGTGTTCTACCTGGTCGGCTCGGCCCGCTTCATCACCACCGCGCCGTTCTACTCGCCCTTCCAGGACC AGTATCCGCCGCGCGACCCGGAAGTCTTCGCCCGGCGCATGGCGATGCTCTCGCAGCGCTACCCGAGCGTGGCCGCCTGGCAGGTATGGAACGAGCCCAA CCTGATCGGCTTCTGGCGGCCCAAGGCCGACCCGGAAGGCTACGCCAAGCTGCTCCAGGCCAGCACCATCGCCCTGCGCATGGTCGACCCGGAGAAGCCG GTGGTTTCCGCCGGCATGGCCTTCTTCAGCGAGATGCCCGACGGCCGCACCATGTTCGACGCCCTCGGCCACCTGGGCGTGGAGAGCCTCGGCACCATCG CCACCTACCACCCCTATACCCAGTTGCCGGAAGGCAACTACCCGTGGAACCTGGACTTCGTCTCCCACGCCAACCAGATCAACCGCGCCCTGCGCAACGC CGGCGTGCCGGCGATCTGGAGCACCGAGTGGGGCTGGTCGGCCTACAAGGGGCCGAAGGAGTTGCAGGACATCATTGGCGTCGAAGGCCAGGCCGACTAC GTGCTGCGTCGCCTGGCGCTGATGAGTGCGCTGGACTACGACCGGATCTTCCTCTTCACCCTCAGCGATCTCGACCAGCGCGCCAGCGTGCGCGACCGCG ACTACGGCCTGCTCGACCTGGACGCCAACCCCAAGCCGGTCTACCTGGCCCTGCAACGCTTCCTCAAGGTCACCGGGCCGAAGCTGCGCCCGGCCGACCC GCCGGTCACCGAGGACCTGCCCGACGGTTCCTTCAGCATCGGCTGGACCCGCGAGGACGGTCGCAACGTCTGGCTGTTCTGGTCGGCCCGCGGCGGCAAC GTGCGCCTGCCGAAGCTCAAGGAGGCCACCCTGCACGATCCGCTCAGCGGCAAGGTCACGCCCTTGAGCGGCAGCGACGGCCTGGAAGTCCCGGTGAAGT CCAGCCTGCAGATGCTGGTCTGGGAGTGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MARKGLYLGGSALLLAVVLLLVFWGRPADAEIQVLKAPRAVVWKDFLGVNAQFLWFSPERYNKQIDRLQDLGLEWVRLDLHWDRLETAEDQYQLASLDQL VKDLEARQLKSVFYLVGSARFITTAPFYSPFQDQYPPRDPEVFARRMAMLSQRYPSVAAWQVWNEPNLIGFWRPKADPEGYAKLLQASTIALRMVDPEKP VVSAGMAFFSEMPDGRTMFDALGHLGVESLGTIATYHPYTQLPEGNYPWNLDFVSHANQINRALRNAGVPAIWSTEWGWSAYKGPKELQDIIGVEGQADY VLRRLALMSALDYDRIFLFTLSDLDQRASVRDRDYGLLDLDANPKPVYLALQRFLKVTGPKLRPADPPVTEDLPDGSFSIGWTREDGRNVWLFWSARGGN VRLPKLKEATLHDPLSGKVTPLSGSDGLEVPVKSSLQMLVWE

References

PubMed

Putative exopolysaccharide synthesis genes influence Pseudomonas aeruginosa biofilm development.
Matsukawa M, Greenberg EP.
J Bacteriol. 2004 Jul;186(14):4449-56.
PMID: 15231776
Identification of psl, a locus encoding a potential exopolysaccharide that is essential for Pseudomonas aeruginosa PAO1 biofilm formation.
Jackson KD, Starkey M, Kremer S, Parsek MR, Wozniak DJ.
J Bacteriol. 2004 Jul;186(14):4466-75.
PMID: 15231778
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE.
Genome Res. 2005 Feb;15(2):321-9.
PMID: 15687295

Alternate Annotations and Cross-References

TIGR

NTL03PA02237

Systomonas

PA2237

RefSeq

NP_250927.1

15597433

KEGG

PA2237

GBrowse

Click here for a GBrowse View of this and other annotations.

PseudoCyc

Click here to view metabolic pathways in PseudoCyc.