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               PA2237               
Key to diagram


View in GBrowse: 10 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name pslG  Alternate Gene Names
Locus tag PA2237 (View 9 updates) Submit an Update GenBank Identifier (GI) 15597433 (Precomputed BLAST results at BLINK )
Genomic location 2461460 - 2462788 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name PslG (Class 1  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Alternate Annotations and Cross-References
TIGR NTL03PA02237
Systomonas PA2237
RefSeq NP_250927.1
GI 15597433
KEGG PA2237
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Putative enzymes
Cell wall / LPS / capsule
GO Accession GO Term Evidence
Molecular Function
Biological Process
GO:0042710 biofilm formation IDA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG3664 XynB, Beta-xylosidase [Carbohydrate transport and metabolism]. Carbohydrate transport and metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Psl exopolysaccharide biosynthesis PseudoCAP
Starch and sucrose metabolism KEGG pae00500 Computationally Generated
Amino sugar and nucleotide sugar metabolism KEGG pae00520 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 43% similar to putative GlcNAc transferase [Bordetella]
Structure Features
Structure Features Explanation of Structure Features Field PS01027: Glycosyl hydrolases family 39 active site
One predicted transmembrane domain
type I export signal computationally predicted by LipoP v.1.0 (cleavage after residue 30)
type I export signal computationally predicted by Phobius (cleavage after residue 30)
type I export signal computationally predicted by SignalP v.3.0 Hidden Markov Models (cleavage after residue 30)
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF01229 Glyco_hydro_39, Glycosyl hydrolases family 39. 59 198 5e-05 23.0
 
Comments
Comments psl cluster plays a role in cell-cell and/or cell-surface interaction in biofilm formation.
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region CCTGACCCTGGCCGGCGGCACCGCCGCGGAAATGGCCTTCGGCGCCGGCGGCAACTACCTCTACCAGTTGAAGGCACAGATCGCCGAGCTTGGCCTGGCC GACGCCATCGACTGGAACCTGAACCTGGCCGCCGCGGACATCCCGCGTACCATCCAGGCCCACCACGTGATGGTCCTGCCGTACCGCGAGTCGAAGAAAC TCGGTCTGCTCGGCCAGCAGCGCGGCACCAGCGGCGCCCTGTCCTGGGCGGCAGCCTGCGGGCGCGGCGCGATCACCTCGGACGCTCGCGCCTTCGCCGA GGAAGTCGCCAGCGGCAACGGCGCCATCTACCCTCAGGGAGACGTGGCAGCCCTCTCCGAACAGTTGTTGAACCTCGCTCGCGAACCCCGCCTGGCACAG GCCTGGGCCGAGCGCGCCGGAGCGATCGGCCATGCGCGGACCTGGCCGCGCACCGCGCTGCGCTTCCAGGCACTGTTCAACGACATGGTGGGAACCGTTC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGGCACGTAAGGGACTCTATCTGGGCGGCAGCGCGCTGCTGCTCGCCGTGGTACTGCTGCTGGTGTTCTGGGGGCGTCCCGCCGACGCCGAGATCCAGG TACTGAAGGCGCCTCGCGCGGTGGTCTGGAAAGACTTCCTCGGGGTCAACGCGCAGTTCCTCTGGTTCAGCCCGGAGCGTTACAACAAGCAGATCGACCG CCTGCAGGACCTGGGGCTGGAGTGGGTGCGCCTGGACCTGCACTGGGACCGCCTGGAAACCGCCGAGGACCAGTACCAGCTGGCCTCCCTCGACCAGTTG GTCAAAGATCTCGAGGCGCGCCAGCTGAAGTCGGTGTTCTACCTGGTCGGCTCGGCCCGCTTCATCACCACCGCGCCGTTCTACTCGCCCTTCCAGGACC AGTATCCGCCGCGCGACCCGGAAGTCTTCGCCCGGCGCATGGCGATGCTCTCGCAGCGCTACCCGAGCGTGGCCGCCTGGCAGGTATGGAACGAGCCCAA CCTGATCGGCTTCTGGCGGCCCAAGGCCGACCCGGAAGGCTACGCCAAGCTGCTCCAGGCCAGCACCATCGCCCTGCGCATGGTCGACCCGGAGAAGCCG GTGGTTTCCGCCGGCATGGCCTTCTTCAGCGAGATGCCCGACGGCCGCACCATGTTCGACGCCCTCGGCCACCTGGGCGTGGAGAGCCTCGGCACCATCG CCACCTACCACCCCTATACCCAGTTGCCGGAAGGCAACTACCCGTGGAACCTGGACTTCGTCTCCCACGCCAACCAGATCAACCGCGCCCTGCGCAACGC CGGCGTGCCGGCGATCTGGAGCACCGAGTGGGGCTGGTCGGCCTACAAGGGGCCGAAGGAGTTGCAGGACATCATTGGCGTCGAAGGCCAGGCCGACTAC GTGCTGCGTCGCCTGGCGCTGATGAGTGCGCTGGACTACGACCGGATCTTCCTCTTCACCCTCAGCGATCTCGACCAGCGCGCCAGCGTGCGCGACCGCG ACTACGGCCTGCTCGACCTGGACGCCAACCCCAAGCCGGTCTACCTGGCCCTGCAACGCTTCCTCAAGGTCACCGGGCCGAAGCTGCGCCCGGCCGACCC GCCGGTCACCGAGGACCTGCCCGACGGTTCCTTCAGCATCGGCTGGACCCGCGAGGACGGTCGCAACGTCTGGCTGTTCTGGTCGGCCCGCGGCGGCAAC GTGCGCCTGCCGAAGCTCAAGGAGGCCACCCTGCACGATCCGCTCAGCGGCAAGGTCACGCCCTTGAGCGGCAGCGACGGCCTGGAAGTCCCGGTGAAGT CCAGCCTGCAGATGCTGGTCTGGGAGTGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MARKGLYLGGSALLLAVVLLLVFWGRPADAEIQVLKAPRAVVWKDFLGVNAQFLWFSPERYNKQIDRLQDLGLEWVRLDLHWDRLETAEDQYQLASLDQL VKDLEARQLKSVFYLVGSARFITTAPFYSPFQDQYPPRDPEVFARRMAMLSQRYPSVAAWQVWNEPNLIGFWRPKADPEGYAKLLQASTIALRMVDPEKP VVSAGMAFFSEMPDGRTMFDALGHLGVESLGTIATYHPYTQLPEGNYPWNLDFVSHANQINRALRNAGVPAIWSTEWGWSAYKGPKELQDIIGVEGQADY VLRRLALMSALDYDRIFLFTLSDLDQRASVRDRDYGLLDLDANPKPVYLALQRFLKVTGPKLRPADPPVTEDLPDGSFSIGWTREDGRNVWLFWSARGGN VRLPKLKEATLHDPLSGKVTPLSGSDGLEVPVKSSLQMLVWE

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA2237 gene of Pseudomonas aeruginosa PAO1, Chromosome (2461460 to 2462788 bp)


Displaying the first five SNPs out of a total of 97 found in all isolate strains ( View all 97 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA7 Transversion g.2461475C>A p.Leu6Ile
 Missense
CTC(Leu) -> ATC(Ile) 		SNP-337797 View
Pseudomonas aeruginosa PA14 Transversion g.2461543C>G
Silent
GCC(Ala) -> GCG(Ala) 		SNP-140300 View
Pseudomonas aeruginosa PA7 Transversion g.2461543C>G
Silent
GCC(Ala) -> GCG(Ala) 		SNP-337798 View
Pseudomonas aeruginosa PA7 Transition g.2461561A>G
Silent
GTA(Val) -> GTG(Val) 		SNP-337799 View
Pseudomonas aeruginosa PA7 Transition g.2461570G>A
Silent
GCG(Ala) -> GCA(Ala) 		SNP-337800 View
Select to view SNPs in a specific strain
SNP Map
Operons/Regulons
Type operon
Genes
GeneProduct
pslA PslA
pslB PslB
pslC PslC
pslD PslD
pslE PslE
pslF PslF
pslG PslG
pslH PslH
pslI PslI
pslJ PslJ
pslK PslL
pslL hypothetical protein
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13575
Genomic Context
Genomic context Explanation of Genomic Context Field - Part of pslABCDEFGHIJKLMNO cluster involved in biofilm development.
PubMed Putative exopolysaccharide synthesis genes influence Pseudomonas aeruginosa biofilm development.
Matsukawa M, Greenberg EP.
J Bacteriol. 2004 Jul;186(14):4449-56.
PMID: 15231776
Identification of psl, a locus encoding a potential exopolysaccharide that is essential for Pseudomonas aeruginosa PAO1 biofilm formation.
Jackson KD, Starkey M, Kremer S, Parsek MR, Wozniak DJ.
J Bacteriol. 2004 Jul;186(14):4466-75.
PMID: 15231778
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE.
Genome Res. 2005 Feb;15(2):321-9.
PMID: 15687295
Assembly and development of the Pseudomonas aeruginosa biofilm matrix.
Ma L, Conover M, Lu H, Parsek MR, Bayles K, Wozniak DJ.
PLoS Pathog. 2009 Mar;5(3):e1000354. Epub 2009 Mar 27.
PMID: 19325879
Genetic and biochemical analyses of the Pseudomonas aeruginosa Psl exopolysaccharide reveal overlapping roles for polysaccharide synthesis enzymes in Psl and LPS production.
Byrd MS, Sadovskaya I, Vinogradov E, Lu H, Sprinkle AB, Richardson SH, Ma L, Ralston B, Parsek MR, Anderson EM, Lam JS, Wozniak DJ.
Mol Microbiol. 2009 Aug;73(4):622-38. Epub 2009 Jul 29.
PMID: 19659934
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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