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               PA2247               
Key to diagram


View in GBrowse: (?) 11 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name bkdA1  Alternate Gene Names
Locus tag PA2247 (View 6 updates) GenBank Identifier (GI) 15597443 (Precomputed BLAST results at BLINK )
Genomic location 2473213 - 2474445 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name 2-oxoisovalerate dehydrogenase (alpha subunit) (Class 2  Explanation of Product Name Confidence)
Alternate Product Names branched-chain alpha-keto acid decarboxylase, 3-methyl-2-oxobutanoate dehydrogenase (lipoamide)
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Amino acid biosynthesis and metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0004739 pyruvate dehydrogenase (acetyl-transferring) activity IEA
Biological Process
GO:0006520 amino acid metabolic process ISS
GO:0006086 acetyl-CoA biosynthetic process from pyruvate IEA
Cellular Component
GO:0045250 cytosolic pyruvate dehydrogenase complex IEA
COG PredictionExplanation of COG predictions
Accession Description Category
COG1071 AcoA, Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit [Energy production and conversion]. Energy production and conversion
TIGRFAM predictionExplanation of TIGRFAM predictions
Accession Name Function Significance Role Sub Role Release No.
TIGR03181 PDH_E1_alph_x pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit 1.6e-98 Energy metabolism Pyruvate dehydrogenase 8.0
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Valine, leucine and isoleucine degradation PseudoCAP
valine degradation I PseudoCyc VALDEG-PWY
Valine, leucine and isoleucine degradation KEGG pae00280 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 1.2.4.4
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 88% similar to bkdA1 gene product of [P. putida]
Most Similar Human Homolog
ENSP00000269980, Percent Identity: 39.22%, E-Value: 2e-55, Ensembl Release: 56.
Description: Transmembrane protein 91
Structure Features
Structure Features Explanation of Structure Features Field PF00676: Dehydrogenase E1 component signature
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00676 E1_dh, Dehydrogenase E1 component. 81 378 3e-94 23.0
 
Genomic Context
Genomic context Explanation of Genomic Context Field - First of 4 ORF gene cluster.
Operons/Regulons
Type operon
Genes
GeneProduct
bkdA1 2-oxoisovalerate dehydrogenase (alpha subunit)
bkdA2 2-oxoisovalerate dehydrogenase (beta subunit)
bkdB branched-chain alpha-keto acid dehydrogenase (lipoamide component)
lpdV lipoamide dehydrogenase-Val
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13577
Microarray Expression Data
NCBI GEO Link
Sequence data
Upstream 500 BP Region CCGGACAGGCGCTCCTCGTCGAGCCGGGCGAAGTAGCCCTGGATGTAGTGTTCCTCTTCCAGGCGGCGTACCCGGCGCAGCGTCGGCGTCAGCGACAGGC CGACCTTCTGGGCGAGATCGCGCCAGCTCAGGCGGCCGTCGTCGGCCAGGGCACGGAGGATTTTCAGGTCGATGCGGTCGAGTTCAGCCATGGTGCGTTG TTTCTCTTGTTAGCGATTCTTGTAGTTCAAACAACCCAATACTGCCGGAATACCTTAGTCAATTTGGAGCAAAAAACTAGCACCCGAAGCCTATACTGAA ATTACAAAAACAAAACCCGAGGGCCTCACCATGAGTGACGTCACCCAGTACGACCGCGCCTGCCGTACCCCGGCATGCCTGCAACCCGCCCCCACCCACA GGACGGGAACCCGCCATCCGCCCAGTCCGCCGCTTCGAGCGCCGTTCGCCCGTTTCTGATCTCTCCAACCGTTTGGCCCGGACCGGGCCGGAGGCCTGTC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGAGTGATTACGAGCCGTTGCGTCTGCATGTCCCGGAGCCCACCGGGCGTCCTGGCTGCAAGACCGACTTTTCCTATCTGCACCTGTCCCCCGCCGGCG AGGTACGCAAGCCGCCGGTGGATGTCGAGCCCGCCGAGACCAGCGACCTGGCCTACAGCCTGGTACGTGTGCTCGACGACGACGGCCACGCCGTCGGTCC CTGGAATCCGCAGCTCAGCAACGAACAACTGCTGCGCGGCATGCGGGCGATGCTCAAGACCCGCCTGTTCGACGCGCGCATGCTCACCGCGCAACGGCAG AAAAAGCTTTCCTTCTATATGCAATGCCTCGGCGAGGAAGCCATCGCCACCGCCCACACCCTGGCCCTGCGCGACGGCGACATGTGCTTTCCGACCTATC GCCAGCAAGGCATCCTGATCACCCGCGAATACCCGCTGGTGGACATGATCTGCCAGCTTCTCTCCAACGAGGCCGACCCGCTCAAGGGCCGCCAGCTGCC GATCATGTACTCGAGCAAGGAGGCAGGTTTCTTCTCCATCTCCGGCAACCTCGCCACCCAGTTCATCCAGGCGGTCGGCTGGGGCATGGCCTCGGCGATC AAGGGCGACACGCGCATCGCCTCGGCCTGGATCGGCGACGGCGCCACCGCCGAGTCGGACTTCCACACCGCCCTCACCTTCGCCCATGTCTACCGCGCGC CGGTAATCCTCAACGTGGTCAACAACCAGTGGGCGATCTCCACCTTCCAGGCCATCGCCGGCGGCGAAGGCACCACCTTCGCCAACCGTGGCGTGGGCTG CGGGATCGCCTCGCTGCGGGTCGACGGCAATGACTTCCTGGCGGTCTACGCCGCCTCCGAGTGGGCCGCCGAGCGCGCCCGGCGCAACCTCGGGCCGAGC CTGATCGAATGGGTCACCTACCGCGCCGGCCCGCACTCGACTTCGGACGACCCGTCCAAGTACCGCCCCGCCGACGACTGGACCAACTTCCCGCTGGGCG ACCCGATCGCCCGCCTGAAGCGGCACATGATCGGCCTCGGCATCTGGTCGGAGGAACAGCACGAAGCCACCCACAAGGCCCTCGAAGCCGAAGTACTGGC CGCGCAGAAACAGGCGGAGAGCCATGGCACCCTGATCGACGGCCGGGTGCCGAGCGCCGCCAGCATGTTCGAGGACGTCTATGCAGAACTGCCGGAGCAC CTGCGCCGGCAACGCCAGGAGCTCGGGGTATGA

BLAST search against this genome or other genomes in database


Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence MSDYEPLRLHVPEPTGRPGCKTDFSYLHLSPAGEVRKPPVDVEPAETSDLAYSLVRVLDDDGHAVGPWNPQLSNEQLLRGMRAMLKTRLFDARMLTAQRQ KKLSFYMQCLGEEAIATAHTLALRDGDMCFPTYRQQGILITREYPLVDMICQLLSNEADPLKGRQLPIMYSSKEAGFFSISGNLATQFIQAVGWGMASAI KGDTRIASAWIGDGATAESDFHTALTFAHVYRAPVILNVVNNQWAISTFQAIAGGEGTTFANRGVGCGIASLRVDGNDFLAVYAASEWAAERARRNLGPS LIEWVTYRAGPHSTSDDPSKYRPADDWTNFPLGDPIARLKRHMIGLGIWSEEQHEATHKALEAEVLAAQKQAESHGTLIDGRVPSAASMFEDVYAELPEH LRRQRQELGV

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
References
PubMed Molecular cloning of genes encoding branched-chain keto acid dehydrogenase of Pseudomonas putida.
Sykes PJ, Burns G, Menard J, Hatter K, Sokatch JR.
J Bacteriol. 1987 Apr;169(4):1619-25.
PMID: 3549697
Similarity of the E1 subunits of branched-chain-oxoacid dehydrogenase from Pseudomonas putida to the corresponding subunits of mammalian branched-chain-oxoacid and pyruvate dehydrogenases.
Burns G, Brown T, Hatter K, Idriss JM, Sokatch JR.
Eur J Biochem. 1988 Sep 15;176(2):311-7.
PMID: 3416875
Transcriptional analysis of the promoter region of the Pseudomonas putida branched-chain keto acid dehydrogenase operon.
Madhusudhan KT, Huang G, Burns G, Sokatch JR.
J Bacteriol. 1990 Oct;172(10):5655-63.
PMID: 2211503
 
Alternate Annotations and Cross-References
TIGR NTL03PA02247
Systomonas PA2247
RefSeq NP_250937.1
GI 15597443
KEGG PA2247
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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