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               PA2248               
Key to diagram


View in GBrowse: (?) 11 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name bkdA2  Alternate Gene Names
Locus tag PA2248 (View 5 updates) GenBank Identifier (GI) 15597444 (Precomputed BLAST results at BLINK )
Genomic location 2474442 - 2475494 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name 2-oxoisovalerate dehydrogenase (beta subunit) (Class 2  Explanation of Product Name Confidence)
Alternate Product Names branched-chain alpha-keto acid decarboxylase, 3-methyl-2-oxobutanoate dehydrogenase
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Amino acid biosynthesis and metabolism
GO Accession GO Term Evidence
Molecular Function
Biological Process
GO:0006520 amino acid metabolic process ISS
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0022 AcoB, Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit [Energy production and conversion]. Energy production and conversion
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Valine, leucine and isoleucine degradation PseudoCAP
valine degradation I PseudoCyc VALDEG-PWY
Valine, leucine and isoleucine degradation KEGG pae00280 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 1.2.4.4
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 94% similar to bkdA2 gene product of [P. putida]
Most Similar Human Homolog
ENSP00000348880, Percent Identity: 41.83%, E-Value: 1e-67, Ensembl Release: 56.
Description: 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial Precursor
Structure Features
Structure Features Explanation of Structure Features Field DM00893: dehydrogenase signature?
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF02779 Transket_pyr, Transketolase, pyrimidine binding domain. 15 192 3e-43 23.0
PF02780 Transketolase_C, Transketolase, C-terminal domain. 224 339 4e-37 23.0
 
Genomic Context
Genomic context Explanation of Genomic Context Field - Second of 4 ORF gene cluster.
Operons/Regulons
Type operon
Genes
GeneProduct
bkdA1 2-oxoisovalerate dehydrogenase (alpha subunit)
bkdA2 2-oxoisovalerate dehydrogenase (beta subunit)
bkdB branched-chain alpha-keto acid dehydrogenase (lipoamide component)
lpdV lipoamide dehydrogenase-Val
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13577
Microarray Expression Data
NCBI GEO Link
Sequence data
Upstream 500 BP Region TGGGCGATCTCCACCTTCCAGGCCATCGCCGGCGGCGAAGGCACCACCTTCGCCAACCGTGGCGTGGGCTGCGGGATCGCCTCGCTGCGGGTCGACGGCA ATGACTTCCTGGCGGTCTACGCCGCCTCCGAGTGGGCCGCCGAGCGCGCCCGGCGCAACCTCGGGCCGAGCCTGATCGAATGGGTCACCTACCGCGCCGG CCCGCACTCGACTTCGGACGACCCGTCCAAGTACCGCCCCGCCGACGACTGGACCAACTTCCCGCTGGGCGACCCGATCGCCCGCCTGAAGCGGCACATG ATCGGCCTCGGCATCTGGTCGGAGGAACAGCACGAAGCCACCCACAAGGCCCTCGAAGCCGAAGTACTGGCCGCGCAGAAACAGGCGGAGAGCCATGGCA CCCTGATCGACGGCCGGGTGCCGAGCGCCGCCAGCATGTTCGAGGACGTCTATGCAGAACTGCCGGAGCACCTGCGCCGGCAACGCCAGGAGCTCGGGGT

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGAATGCCATGAACCCGCAACACGAGAACGCCCAGACGGTCACCAGCATGACCATGATCCAGGCGCTGCGCTCGGCGATGGACATCATGCTCGAGCGCG ACGACGACGTGGTGGTATTCGGCCAGGACGTCGGCTACTTCGGCGGCGTGTTCCGCTGCACCGAAGGCCTGCAGAAGAAATACGGCACCTCGCGGGTGTT CGATGCGCCGATCTCCGAGAGCGGCATCATCGGCGCCGCGGTCGGCATGGGTGCCTACGGCCTGCGCCCGGTGGTGGAGATCCAGTTCGCCGACTACGTC TACCCGGCCTCCGACCAGTTGATCTCCGAGGCGGCGCGCCTGCGCTATCGCTCGGCCGGCGACTTCATCGTGCCGATGACCGTACGCATGCCCTGTGGCG GCGGCATCTACGGCGGGCAAACGCACAGCCAGAGCCCGGAGGCGATGTTCACCCAGGTCTGCGGCCTGCGCACGGTGATGCCGTCCAACCCCTACGACGC CAAGGGCCTGCTGATCGCCTGCATCGAGAACGACGACCCGGTGATCTTCCTCGAGCCCAAGCGCCTCTACAACGGCCCGTTCGATGGCCACCACGACCGC CCGGTGACGCCCTGGTCCAAGCATCCGGCCAGCCAGGTGCCGGACGGCTACTACAAGGTGCCGCTGGACAAGGCGGCGATCGTCCGCCCCGGCGCGGCGC TGACCGTGCTGACCTACGGCACCATGGTCTACGTGGCCCAGGCCGCGGCCGACGAGACCGGCCTGGACGCCGAGATCATCGACCTGCGCAGCCTCTGGCC GCTGGACCTGGAAACCATCGTCGCCTCGGTGAAGAAGACCGGCCGCTGCGTCATCGCCCACGAGGCGACCCGCACCTGCGGGTTCGGCGCCGAGCTGATG TCGCTGGTGCAGGAGCACTGCTTCCACCACCTGGAGGCGCCGATCGAGCGCGTCACCGGTTGGGACACCCCCTACCCGCATGCCCAGGAGTGGGCGTATT TCCCCGGCCCCGCGCGCGTCGGCGCGGCATTCAAGCGTGTGATGGAGGTCTGA

BLAST search against this genome or other genomes in database


Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence MNAMNPQHENAQTVTSMTMIQALRSAMDIMLERDDDVVVFGQDVGYFGGVFRCTEGLQKKYGTSRVFDAPISESGIIGAAVGMGAYGLRPVVEIQFADYV YPASDQLISEAARLRYRSAGDFIVPMTVRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSNPYDAKGLLIACIENDDPVIFLEPKRLYNGPFDGHHDR PVTPWSKHPASQVPDGYYKVPLDKAAIVRPGAALTVLTYGTMVYVAQAAADETGLDAEIIDLRSLWPLDLETIVASVKKTGRCVIAHEATRTCGFGAELM SLVQEHCFHHLEAPIERVTGWDTPYPHAQEWAYFPGPARVGAAFKRVMEV

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
References
PubMed Molecular cloning of genes encoding branched-chain keto acid dehydrogenase of Pseudomonas putida.
Sykes PJ, Burns G, Menard J, Hatter K, Sokatch JR.
J Bacteriol. 1987 Apr;169(4):1619-25.
PMID: 3549697
Similarity of the E1 subunits of branched-chain-oxoacid dehydrogenase from Pseudomonas putida to the corresponding subunits of mammalian branched-chain-oxoacid and pyruvate dehydrogenases.
Burns G, Brown T, Hatter K, Idriss JM, Sokatch JR.
Eur J Biochem. 1988 Sep 15;176(2):311-7.
PMID: 3416875
 
Alternate Annotations and Cross-References
TIGR NTL03PA02248
Systomonas PA2248
RefSeq NP_250938.1
GI 15597444
KEGG PA2248
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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