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               PA2416               
Key to diagram


View in GBrowse: 4 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name treA  Alternate Gene Names
Locus tag PA2416 (View 7 updates) Submit an Update GenBank Identifier (GI) 15597612 (Precomputed BLAST results at BLINK )
Genomic location 2698526 - 2700163 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name periplasmic trehalase precursor (Class 2  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Periplasmic (Class 2Explanation of Localization Confidence)
Periplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA02416
Systomonas PA2416
RefSeq NP_251106.1
GI 15597612
KEGG PA2416
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Carbon compound catabolism
GO Accession GO Term Evidence
Molecular Function
Biological Process
GO:0044248 cellular catabolic process ISS
GO:0015978 carbon utilization by utilization of organic compounds ISS
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG1626 TreA, Neutral trehalase [Carbohydrate transport and metabolism]. Carbohydrate transport and metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Starch and sucrose metabolism PseudoCAP
Starch and sucrose metabolism KEGG pae00500 Computationally Generated
Reactions
EC Number 3.2.1.28
Enzyme Assays
PDF file(s) provided by Sigma-Aldrich:
 Trehalase (3.2.1.28)
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 67% similar to periplasmic trehalase precursor [Escherichia coli]
Most Similar Human Homolog
ENSP00000264029, Percent Identity: 34.22%, E-Value: 6e-66, Ensembl Release: 56.
Description: Trehalase Precursor
Structure Features
Structure Features Explanation of Structure Features Field PS00927: Trehalase signature 1
Type II (lipoprotein) export signal predicted by LipoP (cleavage after residue 22)
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF01204 Trehalase, Trehalase. 63 540 0.0 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region CCGCGCTCTTGCCCCACATCACCTCCGCCGTGGCGAAATAGGTGGCATCGAAGACCAGGGCGGCGACGAAGAAACCGAAGGGAACCGGGTTGAGGACGTT GTAGAAGGCGTCCAGCGGTCTGAAGCGTAGTGCGGTGGCCATTGCGAAACACTCCTCTCGGGAAACGCAGGCTTCCCGACGTGAAGGGACGGGCAGGAGA TTCGAACCGCCGAGGCCCTGCGCAAGGCGCGGGCAGCGGGGAACGGCACGGCGAAACGGCGACTGGGGGGCTCGCGCCCCTTCTGCGACGGACCTGCGCG AAGCGCTGGAGTTCATCCGCAAGGACCTGTCGGAAGCGATAGGTACGCTGGCGATCCTTCCGATGGAACTTCGTGCGCCGCGACTCACCGAAACAGGGGT AGCGCCCTTCCCGGCCCTCCCGCCGGGTTGCCGCGCCGCCCCAGGCGCACCCCGCCGGCGACCGATCCGCGCCCGGCGTTTGCCGAAGAGGAGACAGCCG

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGCCTGATCGAACCGCTCTACCCCGCGCCATGCTCGCGGCCTGGGTCCTGCTCCTGCTGGCGGCCTGCAGCCAGGGCCCGGCGCCTACCCCGCCCGCGT CCTGGGGTTGGCAGGACGCCAGCGGCGAACGCGCGATCGCCCCCGACGAAGCCTATCCCGAGCTGTTCCAGGCGGTGCAGGAAAACCGGCTGTTCAGCGA CCAGAAGCACTTCGTCGATGCGCTCCCGCTGCGCGAGCCTGCACGGATCCGCGCCGACTACCTGCGCGAGAGGGAGCGGCCCGGCTTCGACCTGCGCGCC TTCGTCGGCCGCAACTTCGAGGAGTCCGGCAGCGTCGAGACCGCCCCGCCGGAAGCGGGTGCCGATCTCGCCAGCCACATCAGCGACCTGTGGCCGGCGT TGACCCGCCACTACGAGCAGGTGCCGGCCCACAGCAGCCTGCTGCCGCTGCCGAAACCCTACGTGGTCCCCGGCGGACGCTTCCGCGAGGTGTACTACTG GGACTCCTACTTCACCATGCTCGGCCTTGCGGAGAGCGGGCAGCACCAGCGGGTGCGCGACATGCTCGACAACTTCGCCTACCTGATCGATACCTACGGG CACATTCCCAACGGTAACCGCAGCTACTACCTGAGCCGTTCGCAGCCGCCGTTCTTCGCCTACATGGTGGATCTCCAGGCAAGACGCGAAGGCGACGCCG CCTACCGGCGCTACCTGCCGCAGCTACAGAAGGAATACGCCTACTGGATGGAAGGCTCCGCCGGGCTGCGCCCCAACGAGGCGCGCCTGCACGTGGTGAA ACTGGCCGACGGAAGCCTGCTGAACCGCTACTGGGACAACCGTGACACACCGCGCCAGGAGTCCTTCCTGGAGGACCGGGCGACCGCCGCGCGCGCCCCG CAGCGCCCCGCGGGCGAGGTCTATCGCGACCTGCGGGCCGGCGCGGAAAGCGGCTGGGACTTCTCCTCCCGCTGGCTGGACGACGGCCGCGAACTGGCCA GCATCCGCACCACCGCCATCGTCCCGGTGGACCTCAACGCCCTGCTCTACCATCTCGAACGAATCATCGCCAAGGCCTGCGCCAGCAGCGCGCTGAAGGC CTGCGAGCAAGGCTACGGCGCCCGCGCGGAGAAGCGACGACAGGCCATCGAAGACCACCTCTGGCACCCGGCGGGCTACTACGCCGACTACGACTGGCAA CGCCGGCGGCCCATCGAGCGGATCAACGCCGCCAGCCTCTTCCCGCTATTCACCGGGCTGGCCTCCGCCGAACGCGCCGGTCGTACCGCCGACAGCGTCG CCGCCCAACTGCTGCGCCCGGGCGGACTGGCCACCACCACCCGTGCCAGCGGCCAGCAATGGGACGAGCCGAACGGCTGGGCACCCTTGCAGTGGGTCGC CGTACAGGGCCTGCGCGCCTACGGCCGGGACGCCCTGGCGGAAGACATCGGCCGGCGATTCCTCGCCCAGGTGCAGCAGGTCTACGACCGCGAAGGAAAA CTGGTGGAGAAATACGACATCAGCGGCAACCAGGGCGGCGGCGGCGGTGGCGAGTACCCGTTGCAGGACGGCTTCGGCTGGTCCAACGGCGTGACCCTGC AACTGCTCCGACTCTACGGCCCGGGCGCCGGTCGCTGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MPDRTALPRAMLAAWVLLLLAACSQGPAPTPPASWGWQDASGERAIAPDEAYPELFQAVQENRLFSDQKHFVDALPLREPARIRADYLRERERPGFDLRA FVGRNFEESGSVETAPPEAGADLASHISDLWPALTRHYEQVPAHSSLLPLPKPYVVPGGRFREVYYWDSYFTMLGLAESGQHQRVRDMLDNFAYLIDTYG HIPNGNRSYYLSRSQPPFFAYMVDLQARREGDAAYRRYLPQLQKEYAYWMEGSAGLRPNEARLHVVKLADGSLLNRYWDNRDTPRQESFLEDRATAARAP QRPAGEVYRDLRAGAESGWDFSSRWLDDGRELASIRTTAIVPVDLNALLYHLERIIAKACASSALKACEQGYGARAEKRRQAIEDHLWHPAGYYADYDWQ RRRPIERINAASLFPLFTGLASAERAGRTADSVAAQLLRPGGLATTTRASGQQWDEPNGWAPLQWVAVQGLRAYGRDALAEDIGRRFLAQVQQVYDREGK LVEKYDISGNQGGGGGGEYPLQDGFGWSNGVTLQLLRLYGPGAGR

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA2416 gene of Pseudomonas aeruginosa PAO1, Chromosome (2698526 to 2700163 bp)


Displaying the first five SNPs out of a total of 181 found in all isolate strains ( View all 181 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA7 Transition g.2698531T>C
Silent
CCT(Pro) -> CCC(Pro) 		SNP-281632 View
Pseudomonas aeruginosa PA7 Transversion g.2698533A>C p.Asp3Ala
 Missense
GAT(Asp) -> GCT(Ala) 		SNP-281633 View
Pseudomonas aeruginosa PA7 Transition g.2698541G>A p.Ala6Thr
 Missense
GCT(Ala) -> ACT(Thr) 		SNP-281634 View
Pseudomonas aeruginosa PACS2 Transition g.2698543T>C
Silent
GCT(Ala) -> GCC(Ala) 		SNP-125952 View
Pseudomonas aeruginosa C3719 Transition g.2698546A>G
Not determined
CTA(Leu) -> CTG() 		SNP-31703 View
Select to view SNPs in a specific strain
SNP Map
PubMed Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE.
Genome Res. 2005 Feb;15(2):321-9.
PMID: 15687295
Trehalase of Escherichia coli. Mapping and cloning of its structural gene and identification of the enzyme as a periplasmic protein induced under high osmolarity growth conditions.
Boos W, Ehmann U, Bremer E, Middendorf A, Postma P.
J Biol Chem. 1987 Sep 25;262(27):13212-8.
PMID: 2820965
Osmotic induction of the periplasmic trehalase in Escherichia coli K12: characterization of the treA gene promoter.
Repoila F, Gutierrez C.
Mol Microbiol. 1991 Mar;5(3):747-55.
PMID: 1710760
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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