Pseudomonas Genome Database - Annotation for PA2517

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PA2517

Key to diagram

View in GBrowse:

11 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Operons/Regulons
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name	xylY	Alternate Gene Names
Locus tag	PA2517 (View 5 updates) Submit an Update	GenBank Identifier (GI)	15597713 (Precomputed BLAST results at BLINK )
Genomic location	2834690 - 2834202 (-)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG003047
Product Information
Product Name	toluate 1,2-dioxygenase beta subunit (Class 2 )
Alternate Product Names
Subcellular localization	Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA02517
Systomonas	PA2517
RefSeq	NP_251207.1
GI	15597713
KEGG	PA2517
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Operons/Regulons

Type

operon

Genes

Gene	Product
xylL	cis-1,2-dihydroxycyclohexa-3,4-diene carboxylate dehydrogenase
xylZ	toluate 1,2-dioxygenase electron transfer component
xylY	toluate 1,2-dioxygenase beta subunit
xylX	toluate 1,2-dioxygenase alpha subunit

Evidence

Computationally Predicted

PubMed

18988623

Cross-Reference

DOOR-13632

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Carbon compound catabolism

GO Accession

GO Term

Evidence

Molecular Function

Biological Process

GO:0044248	cellular catabolic process	ISS
GO:0015978	carbon utilization by utilization of organic compounds	ISS
GO:0019439	aromatic compound catabolic process	ISS

Cellular Component

COG Prediction

Accession	Description	Category
COG5517	COG5517, Small subunit of phenylpropionate dioxygenase [Secondary metabolites biosynthesis, transport, and catabolism].	Secondary metabolites biosynthesis, transport, and catabolism

TIGRFAM prediction

Accession	Name	Function	Significance	Role	Sub Role	Release No.
TIGR03232	benzo_1_2_benB	benzoate 1,2-dioxygenase, small subunit	9.4e-101	No Data	No Data	8.0

Pathways

Name	Database	Xref
Aromatic compound catabolism	PseudoCAP
Benzoate degradation via hydroxylation	KEGG	pae00362	Computationally Generated
Fluorobenzoate degradation	KEGG	pae00364	Computationally Generated
Toluene and xylene degradation	KEGG	pae00622	Computationally Generated
Benzoate degradation via CoA ligation	KEGG	pae00632	Computationally Generated
Metabolic pathways	KEGG	pae01100	Computationally Generated

Reactions

EC Number

1.4.12.-

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

89% similar to toluate 1,2-dioxygenase beta subunit [Plasmid pWW0]

Structure Features

PF00866: Ring hydroxylating beta subunit

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF00866	Ring_hydroxyl_B, Ring hydroxylating beta subunit.	14	155	4e-44	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

TTCGGCGAGGCCCGCGCCGACTGGATGATCGAGAACTCGCGCAACCTCTGCCTGTACCCCAACGTGTACCTGATGGACCAGTTCAGCTCGCAGATCCGCA TCGCCCGGCCGCTGTCGGTGGACCGCACGGAAATCACCATCTACTGCATCGCGCCGAAAGGCGAGAGCGCCGAGGCGCGCGCCCGGCGCATCCGCCAGTA CGAGGACTTCTTCAACGTCAGCGGCATGGCCACCCCGGACGACCTGGAGGAGTTCCGTTCCTGCCAGCAGGGCTACCAGGGCAGCGTGGTCGGCTGGAAC GACCTGTCGCGCGGCGCCGAACACTGGATAGAGGGCGCCGACGCGGCGGCCGCGGCCATCGACCTGCGGCCGCGCCTGAGCGGCGTGCGCACCGAGGACG AAGGGCTGTTCGTATTGCAGCATCGCTACTGGCAGGAGGCGCTGCTCGCCGCCCTGGAGCGCGAACGGACGCAGCGCGTGGCGCTGCGGGAGGTGGCGTC

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGAGCCTGGGCTACGACGCCCTGCGCGATTTCCTCTACCGCGAGGCGCGCTATCTCGACGACAAGGATTGGGACGCCTGGCTGGCGTTGTACGCCGCGG ACGCCAGTTTCTGGATGCCGTCCTGGGACGACCGCGACCAGCTCACCGAGGACCCGCAGCGGGAAATCTCGCTGATCTGGTACGGCAACCGCGGCGGCCT GGAGGACCGGGTATTCCGCATCCGCACCGAGCGCTCCAGCGCGACCCTGCCGGACACCCGCACCTCGCACAACCTGAGCAACATCGAACTGCTCGGCGAG GACGACGGCATCTGCCGCGTGCGCTGCAACTGGCACACCCTGAGCTATCGCTACAAGACCGTGGACAGCTACTTCGGCACCACCTTCTACGACCTCGACG TACGCGGCGAGAGTCCGCTGATCAAGGCCAAGAAAGTGATCCTGAAGAACGACTACGTTCGCCAGCTGATCGACGTCTACCACGTCTGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MSLGYDALRDFLYREARYLDDKDWDAWLALYAADASFWMPSWDDRDQLTEDPQREISLIWYGNRGGLEDRVFRIRTERSSATLPDTRTSHNLSNIELLGE DDGICRVRCNWHTLSYRYKTVDSYFGTTFYDLDVRGESPLIKAKKVILKNDYVRQLIDVYHV

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA2517 gene of Pseudomonas aeruginosa PAO1, Chromosome (2834202 to 2834690 bp)

Displaying the first five SNPs out of a total of 38 found in all isolate strains ( View all 38 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa PA7	Transition	g.2834216C>T	p.Val159Ile	Missense GTC(Val) -> ATC(Ile)	SNP-415124	View
Pseudomonas aeruginosa PA7	Transition	g.2834225G>A		Silent CTG(Leu) -> TTG(Leu)	SNP-415125	View
Pseudomonas aeruginosa PA7	Transversion	g.2834232A>T		Silent GTT(Val) -> GTA(Val)	SNP-415126	View
Pseudomonas aeruginosa PA7	Transition	g.2834241G>A		Silent AAC(Asn) -> AAT(Asn)	SNP-415127	View
Pseudomonas aeruginosa C3719	Transition	g.2834275G>A	p.Pro139Leu	Missense CCG(Pro) -> CTG(Leu)	SNP-22757	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Molecular and functional analysis of the TOL plasmid pWWO from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway.
Franklin FC, Bagdasarian M, Bagdasarian MM, Timmis KN.
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7458-62.
PMID: 6950388