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               PA2518               
Key to diagram


View in GBrowse: 11 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name xylX  Alternate Gene Names
Locus tag PA2518 (View 5 updates) Submit an Update GenBank Identifier (GI) 15597714 (Precomputed BLAST results at BLINK )
Genomic location 2836054 - 2834687 (-) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name toluate 1,2-dioxygenase alpha subunit (Class 2  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA02518
Systomonas PA2518
RefSeq NP_251208.1
GI 15597714
KEGG PA2518
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
xylL cis-1,2-dihydroxycyclohexa-3,4-diene carboxylate dehydrogenase
xylZ toluate 1,2-dioxygenase electron transfer component
xylY toluate 1,2-dioxygenase beta subunit
xylX toluate 1,2-dioxygenase alpha subunit
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13632
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Carbon compound catabolism
GO Accession GO Term Evidence
Molecular Function
Biological Process
GO:0044248 cellular catabolic process ISS
GO:0015978 carbon utilization by utilization of organic compounds ISS
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG4638 HcaE, Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit [Inorganic ion transport and metabolism / General function prediction only]. Inorganic ion transport and metabolism / General function prediction only
TIGRFAM predictionExplanation of TIGRFAM predictions
Accession Name Function Significance Role Sub Role Release No.
TIGR03229 benzo_1_2_benA benzoate 1,2-dioxygenase, large subunit 0 Energy metabolism Other 8.0
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Aromatic compound catabolism PseudoCAP
Benzoate degradation via hydroxylation KEGG pae00362 Computationally Generated
Fluorobenzoate degradation KEGG pae00364 Computationally Generated
Toluene and xylene degradation KEGG pae00622 Computationally Generated
Benzoate degradation via CoA ligation KEGG pae00632 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 1.14.12.10
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 83% similar to toluate 1,2-dioxygenase alpha subunit [Plasmid pWW0]
Structure Features
Structure Features Explanation of Structure Features Field PD02042: IRON-SULFUR ELECTRON TRANSPORT AROMATIC HYDROCARBONS CATABOLISM FERREDOXIN SYSTEM COMPONENT SUBUNIT
PS00570: Bacterial ring hydroxylating dioxygenases alpha-subunit signature
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00355 Rieske, Rieske [2Fe-2S] domain. 45 134 3e-20 23.0
PF00848 Ring_hydroxyl_A, Ring hydroxylating alpha subunit (catalytic domain). 168 382 1e-30 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region AGCAAGCTGCTGACCCTGCTGAAGACCAACGTCAGCCGCGAGGACCCCGGCGATGCGCAGGTCGTCTTCGCCCGCCTCGATGCCTACATCCGCCGCCATC TGCAGAGCGAGATCGAGGTCGCGGCGCTGGCCGAGCAGGCGCACATGAGCACCCGTTCGCTGTACGCGCTGTTCGAGCGCCAGCTTGGCGAGAGCCCTCG GCAATACATTCGCCGGCTGCGCCTGGAGCGCATCCGCGCCTGCCTGGAAGACCCCGGCTGCCGGGTACGCAACCTCACCGAACTGGCCCTGGACTTCGGT TTCGCCCACCTCGGCCGCTTCGCCGAGCAATATCGCCGGCAGTTCGGCGAGCTGCCTTCGGAAACCCTGCGCCGGCGCGCCTAGCGCCGACCGCCACGGC GTGCAGGAATCGGCCAGCGATCCGCAGGCAACGGATATCCCCAGCAGCACGCGCTACCTAACCTGACCGGGCCTGAACAATAACAACGGAGGCCCCGGCC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGTCCCTGGGTAGCGATTACTTGCGTTCTCTGCTCGAGGAAGACCCCGAGCGCGGCGTCTACCGCTGCAAGCGGGAGATGTTCACCGATCCACGCCTGT TCGAACTGGAGATGAAGCACATCTTCGAGGGCAACTGGGTCTATCTCGCCCACGAAAGCCAGGTTGCCGGAGTCAACGACTACCTGACCACCCAGATCGG CCGCCAGTCGATCGTCATCGCGCGCAACCGCGACGGCCAGCTCAACGCCTTCATCAACGCCTGCAGCCACCGTGGCGCGATGCTCTGCCGGCACAAGAGC GGCAATCGCAGCAGCTATACCTGTCCGTTCCACGGCTGGACCTTCAACAACTCCGGCAAGCTGCTCAAGGTCAAGGACCCGGCCGAGGCCGGCTACCCGC AGGGCTTCAACTGCGAGGGCTCGCACGACCTGACCCGGGTGGCGCGCTTCGAGTCCTACCGTGGCTTCCTGTTCGGCAGCCTCAACCCCGATGTCAGGCC GCTGGCCGAGCACCTGGGCGAGTCGGCGAAGATCATCGACATGATCGTCGACCAGTCGCCGGAAGGCCTCGAGGTGCTGCGCGGCTCTTCCAGCTATGTC TACGAAGGCAACTGGAAGCTCACCGCCGAGAACGGCGCCGACGGCTATCACGTCAGTTCGGTGCACTGGAACTACGCGGCGACCCAGAGCCAGCGCCAGC AGCGCGACGCCGCGGACCCGCTGCGGACCATGAGCGCCGCGGGCTGGGCCCGGCAGGGCGGCGGCTTCTACTCGTTCGAGCATGGCCACATGCTGCTCTG GAGTCGCTGGGCCAACCCCGAGGACCGGCCGGCCTTCGAGCGCCGCGCCGAGCTTGCCCGCGACTTCGGCGAGGCCCGCGCCGACTGGATGATCGAGAAC TCGCGCAACCTCTGCCTGTACCCCAACGTGTACCTGATGGACCAGTTCAGCTCGCAGATCCGCATCGCCCGGCCGCTGTCGGTGGACCGCACGGAAATCA CCATCTACTGCATCGCGCCGAAAGGCGAGAGCGCCGAGGCGCGCGCCCGGCGCATCCGCCAGTACGAGGACTTCTTCAACGTCAGCGGCATGGCCACCCC GGACGACCTGGAGGAGTTCCGTTCCTGCCAGCAGGGCTACCAGGGCAGCGTGGTCGGCTGGAACGACCTGTCGCGCGGCGCCGAACACTGGATAGAGGGC GCCGACGCGGCGGCCGCGGCCATCGACCTGCGGCCGCGCCTGAGCGGCGTGCGCACCGAGGACGAAGGGCTGTTCGTATTGCAGCATCGCTACTGGCAGG AGGCGCTGCTCGCCGCCCTGGAGCGCGAACGGACGCAGCGCGTGGCGCTGCGGGAGGTGGCGTCATGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MSLGSDYLRSLLEEDPERGVYRCKREMFTDPRLFELEMKHIFEGNWVYLAHESQVAGVNDYLTTQIGRQSIVIARNRDGQLNAFINACSHRGAMLCRHKS GNRSSYTCPFHGWTFNNSGKLLKVKDPAEAGYPQGFNCEGSHDLTRVARFESYRGFLFGSLNPDVRPLAEHLGESAKIIDMIVDQSPEGLEVLRGSSSYV YEGNWKLTAENGADGYHVSSVHWNYAATQSQRQQRDAADPLRTMSAAGWARQGGGFYSFEHGHMLLWSRWANPEDRPAFERRAELARDFGEARADWMIEN SRNLCLYPNVYLMDQFSSQIRIARPLSVDRTEITIYCIAPKGESAEARARRIRQYEDFFNVSGMATPDDLEEFRSCQQGYQGSVVGWNDLSRGAEHWIEG ADAAAAAIDLRPRLSGVRTEDEGLFVLQHRYWQEALLAALERERTQRVALREVAS

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA2518 gene of Pseudomonas aeruginosa PAO1, Chromosome (2834687 to 2836054 bp)


Displaying the first five SNPs out of a total of 105 found in all isolate strains ( View all 105 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA7 Transition g.2834722T>C p.Thr445Ala
 Missense
ACG(Thr) -> GCG(Ala) 		SNP-415152 View
Pseudomonas aeruginosa PA7 Transition g.2834724C>T p.Arg444Gln
 Missense
CGG(Arg) -> CAG(Gln) 		SNP-415153 View
Pseudomonas aeruginosa PA7 Transition g.2834726T>C
Silent
GAA(Glu) -> GAG(Glu) 		SNP-415154 View
Pseudomonas aeruginosa PA7 Transversion g.2834738G>C
Silent
GCC(Ala) -> GCG(Ala) 		SNP-415155 View
Pseudomonas aeruginosa PA7 Transition g.2834768A>G
Silent
CAT(His) -> CAC(His) 		SNP-415156 View
Select to view SNPs in a specific strain
SNP Map
PubMed Genetic analysis of a relaxed substrate specificity aromatic ring dioxygenase, toluate 1,2-dioxygenase, encoded by TOL plasmid pWW0 of Pseudomonas putida.
Harayama S, Rekik M, Timmis KN.
Mol Gen Genet. 1986 Feb;202(2):226-34.
PMID: 3010045
The meta cleavage operon of TOL degradative plasmid pWW0 comprises 13 genes.
Harayama S, Rekik M.
Mol Gen Genet. 1990 Mar;221(1):113-20.
PMID: 2183008
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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